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1.
J Neurosci ; 34(16): 5447-53, 2014 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-24741035

RESUMO

There are ∼20 types of retinal ganglion cells (RGCs) in mice, each of which has distinct molecular, morphological, and physiological characteristics. Each RGC type sends axon projections to specific brain areas that execute light-dependent behaviors. Here, we show that the T-box transcription factor Tbr2 is required for the development of several RGC types that participate in non-image-forming circuits. These types are molecularly distinct, project to non-image-forming targets, and include intrinsically photosensitive RGCs. Tbr2 mutant mice have reduced retinal projections to non-image-forming nuclei and an attenuated pupillary light reflex. These data demonstrate that Tbr2 acts to execute RGC type choice and/or survival in a set of RGCs that mediates light-induced subconscious behaviors.


Assuntos
Axônios/fisiologia , Regulação da Expressão Gênica/fisiologia , Pupila/fisiologia , Reflexo/fisiologia , Proteínas com Domínio T/metabolismo , Vias Visuais/fisiologia , Fatores Etários , Animais , Animais Recém-Nascidos , Caderinas/genética , Calbindina 2/genética , Calbindina 2/metabolismo , Feminino , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Masculino , Camundongos , Camundongos Transgênicos , Mutação/genética , Receptores de Dopamina D4/genética , Receptores de Dopamina D4/metabolismo , Reflexo/genética , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/fisiologia , Proteínas com Domínio T/genética
2.
Proc Natl Acad Sci U S A ; 108(47): 19060-5, 2011 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-22065784

RESUMO

Topographic maps are the primary means of relaying spatial information in the brain. Understanding the mechanisms by which they form has been a goal of experimental and theoretical neuroscientists for decades. The projection of the retina to the superior colliculus (SC)/tectum has been an important model used to show that graded molecular cues and patterned retinal activity are required for topographic map formation. Additionally, interaxon competition has been suggested to play a role in topographic map formation; however, this view has been recently challenged. Here we present experimental and computational evidence demonstrating that interaxon competition for target space is necessary to establish topography. To test this hypothesis experimentally, we determined the nature of the retinocollicular projection in Math5 (Atoh7) mutant mice, which have severely reduced numbers of retinal ganglion cell inputs into the SC. We find that in these mice, retinal axons project to the anteromedialj portion of the SC where repulsion from ephrin-A ligands is minimized and where their attraction to the midline is maximized. This observation is consistent with the chemoaffinity model that relies on axon-axon competition as a mapping mechanism. We conclude that chemical labels plus neural activity cannot alone specify the retinocollicular projection; instead axon-axon competition is necessary to create a map. Finally, we present a mathematical model for topographic mapping that incorporates molecular labels, neural activity, and axon competition.


Assuntos
Axônios/fisiologia , Modelos Biológicos , Percepção Espacial/fisiologia , Vias Visuais/fisiologia , Percepção Visual/fisiologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fluorescência , Humanos , Camundongos , Camundongos Mutantes , Proteínas do Tecido Nervoso/genética , Retina/fisiologia , Estatísticas não Paramétricas , Colículos Superiores/fisiologia
3.
Proc Natl Acad Sci U S A ; 106(29): 12168-73, 2009 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-19581577

RESUMO

Frontotemporal dementia (FTD) is the most common form of dementia before 60 years of age. Rare pathogenic mutations in CHMP2B, which encodes a component of the endosomal sorting complex required for transport (ESCRT-III), are associated with FTD linked to chromosome 3 (FTD3). Animal models of FTD3 have not yet been reported, and what signaling pathways are misregulated by mutant CHMP2B in vivo is unknown. Here we report the establishment of a Drosophila model of FTD3 and show the genetic interactions between mutant CHMP2B and other components of ESCRT. Through an unbiased genome-wide screen, we identified 29 modifier loci and found that serpin5 (Spn5), a largely uncharacterized serine protease inhibitor, suppresses the melanization phenotype induced by mutant CHMP2B in the fly eye. We also found that Spn5 is a negative regulator of the Toll pathway and functions extracellularly, likely by blocking the proteolytic activation of Spaetzle, the Toll receptor ligand. Moreover, Spn5 inhibited activation of the Toll pathway by mutant CHMP2B. Our findings identify Spn5 as a regulator of the Toll pathway and CHMP2B toxicity and show that the Toll pathway is a major signaling pathway misregulated by mutant CHMP2B in vivo. This fly model will be useful to further dissect genetic pathways that are potentially relevant to the pathogenesis and treatment of FTD.


Assuntos
Demência/genética , Proteínas de Drosophila/toxicidade , Drosophila melanogaster/genética , Testes Genéticos , Serpinas/metabolismo , Transdução de Sinais/efeitos dos fármacos , Receptores Toll-Like/metabolismo , Proteínas de Transporte Vesicular/toxicidade , Animais , Transporte Biológico/efeitos dos fármacos , Demência/patologia , Modelos Animais de Doenças , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/efeitos dos fármacos , Endossomos/efeitos dos fármacos , Endossomos/metabolismo , Espaço Extracelular/efeitos dos fármacos , Espaço Extracelular/metabolismo , Olho/efeitos dos fármacos , Olho/patologia , Genes Dominantes , Genes de Insetos , Íntrons/genética , Proteínas Mutantes/toxicidade , Fenótipo , Supressão Genética/efeitos dos fármacos , Proteínas de Transporte Vesicular/metabolismo
4.
Curr Biol ; 16(10): 1006-11, 2006 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-16713958

RESUMO

The diversity of neuronal cells, especially in the size and shape of their dendritic and axonal arborizations, is a striking feature of the mature nervous system. Dendritic branching is a complex process, and the underlying signaling mechanisms remain to be further defined at the mechanistic level. Here we report the identification of shrub mutations that increased dendritic branching. Single-cell clones of shrub mutant dendritic arborization (DA) sensory neurons in Drosophila larvae showed ectopic dendritic and axonal branching, indicating a cell-autonomous function for shrub in neuronal morphogenesis. shrub encodes an evolutionarily conserved coiled-coil protein homologous to the yeast protein Snf7, a key component in the ESCRT-III (endosomal sorting complex required for transport) complex that is involved in the formation of endosomal compartments known as multivesicular bodies (MVBs). We found that mouse orthologs could substitute for Shrub in mutant Drosophila embryos and that loss of Shrub function caused abnormal distribution of several early or late endosomal markers in DA sensory neurons. Our findings demonstrate that the novel coiled-coil protein Shrub functions in the endosomal pathway and plays an essential role in neuronal morphogenesis.


Assuntos
Dendritos/fisiologia , Proteínas de Drosophila/fisiologia , Drosophila/crescimento & desenvolvimento , Proteínas do Tecido Nervoso/fisiologia , Sistema Nervoso/crescimento & desenvolvimento , Animais , Axônios/fisiologia , Drosophila/embriologia , Drosophila/genética , Proteínas de Drosophila/genética , Desenvolvimento Embrionário , Endossomos/fisiologia , Camundongos , Mutação , Proteínas do Tecido Nervoso/genética
5.
J Comp Neurol ; 527(1): 225-235, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-28078709

RESUMO

Retinal ganglion cells (RGCs) are tasked with transmitting all light information from the eye to the retinal recipient areas of the brain. RGCs can be classified into many different types by morphology, gene expression, axonal projections, and functional responses to different light stimuli. Ultimately, these classification systems should be unified into an all-encompassing taxonomy. Toward that end, we show here that nearly all RGCs express either Islet-2 (Isl2), Tbr2, or a combination of Satb1 and Satb2. We present gene expression data supporting the hypothesis that Satb1 and Satb2 are expressed in ON-OFF direction-selective (DS) RGCs, complementing our previous work demonstrating that RGCs that express Isl2 and Tbr2 are non-DS and non-image-forming, respectively. Expression of these transcription factors emerges at distinct embryonic ages and only in postmitotic cells. Finally, we demonstrate that these transcription factor-defined RGC classes are born throughout RGC genesis.


Assuntos
Células Ganglionares da Retina/classificação , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/metabolismo , Fatores de Transcrição/biossíntese , Animais , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos
6.
Dev Neurobiol ; 75(6): 584-93, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25649160

RESUMO

In the retinocollicular projection, the axons from functionally distinct retinal ganglion cell (RGC) types form synapses in a stereotypical manner along the superficial to deep axis of the superior colliculus (SC). Each lamina contains an orderly topographic map of the visual scene but different laminae receive inputs from distinct sets of RGCs, and inputs to each lamina are aligned with the others to integrate parallel streams of visual information. To determine the relationship between laminar organization and topography of physiologically defined RGC types, we used genetic and anatomical axon tracing techniques in wild type and ephrin-A mutant mice. We find that adjacent RGCs of the same physiological type can send axons to both ectopic and normal topographic locations, supporting a penetrance model for ephrin-A independent mapping cues. While the overall laminar organization in the SC is unaffected in ephrin-A2/A5 double mutant mice, analysis of the laminar locations of ectopic terminations shows that the topographic maps of different RGC types are misaligned. These data lend support to the hypothesis that the retinocollicular projection is a superimposition of a number of individual two-dimensional topographic maps that originate from specific types of RGCs, require ephrin-A signaling, and form independently of the other maps.


Assuntos
Mapeamento Encefálico , Efrinas/metabolismo , Células Ganglionares da Retina/fisiologia , Vias Visuais , Aminoácidos/metabolismo , Animais , Encéfalo/anatomia & histologia , Encéfalo/metabolismo , Efrinas/genética , Camundongos , Camundongos Transgênicos , Mutação/genética , Células Ganglionares da Retina/classificação , Colículos Superiores/metabolismo , Vias Visuais/citologia , Vias Visuais/fisiologia
7.
Neural Dev ; 9: 2, 2014 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-24495295

RESUMO

BACKGROUND: There are numerous functional types of retinal ganglion cells (RGCs), each participating in circuits that encode a specific aspect of the visual scene. This functional specificity is derived from distinct RGC morphologies and selective synapse formation with other retinal cell types; yet, how these properties are established during development remains unclear. Islet2 (Isl2) is a LIM-homeodomain transcription factor expressed in the developing retina, including approximately 40% of all RGCs, and has previously been implicated in the subtype specification of spinal motor neurons. Based on this, we hypothesized that Isl2+ RGCs represent a related subset that share a common function. RESULTS: We morphologically and molecularly characterized Isl2+ RGCs using a transgenic mouse line that expresses GFP in the cell bodies, dendrites and axons of Isl2+ cells (Isl2-GFP). Isl2-GFP RGCs have distinct morphologies and dendritic stratification patterns within the inner plexiform layer and project to selective visual nuclei. Targeted filling of individual cells reveals that the majority of Isl2-GFP RGCs have dendrites that are monostratified in layer S3 of the IPL, suggesting they are not ON-OFF direction-selective ganglion cells. Molecular analysis shows that most alpha-RGCs, indicated by expression of SMI-32, are also Isl2-GFP RGCs. Isl2-GFP RGCs project to most retino-recipient nuclei during early development, but specifically innervate the dorsal lateral geniculate nucleus and superior colliculus (SC) at eye opening. Finally, we show that the segregation of Isl2+ and Isl2- RGC axons in the SC leads to the segregation of functional RGC types. CONCLUSIONS: Taken together, these data suggest that Isl2+ RGCs comprise a distinct class and support a role for Isl2 as an important component of a transcription factor code specifying functional visual circuits. Furthermore, this study describes a novel genetically-labeled mouse line that will be a valuable resource in future investigations of the molecular mechanisms of visual circuit formation.


Assuntos
Axônios/ultraestrutura , Dendritos/ultraestrutura , Proteínas com Homeodomínio LIM/genética , Células Ganglionares da Retina/classificação , Colículos Superiores/citologia , Fatores de Transcrição/genética , Animais , Axônios/metabolismo , Dendritos/metabolismo , Corpos Geniculados/citologia , Camundongos , Camundongos Transgênicos , Vias Neurais/citologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/metabolismo
9.
Dev Biol ; 247(1): 76-88, 2002 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-12074553

RESUMO

To study the roles of intracellular factors in neuronal morphogenesis, we used the mosaic analysis with a repressible cell marker (MARCM) technique to visualize identifiable single multiple dendritic (MD) neurons in living Drosophila larvae. We found that individual neurons in the peripheral nervous system (PNS) developed clear morphological polarity and diverse dendritic branching patterns in larval stages. Each MD neuron in the same dorsal cluster developed a unique dendritic field, suggesting that they have specific physiological functions. Single-neuron analysis revealed that Flamingo did not affect the general dendritic branching patterns in postmitotic neurons. Instead, Flamingo limited the extension of one or more dorsal dendrites without grossly affecting lateral branches. The dendritic overextension phenotype was partially conferred by the precocious initiation of dorsal dendrites in flamingo mutant embryos. In addition, Flamingo is required cell autonomously to promote axonal growth and to prevent premature axonal branching of PNS neurons. Our molecular analysis also indicated that the amino acid sequence near the first EGF motif is important for the proper localization and function of Flamingo. These results demonstrate that Flamingo plays a role in early neuronal differentiation and exerts specific effects on dendrites and axons.


Assuntos
Caderinas/genética , Drosophila/embriologia , Sistema Nervoso/embriologia , Animais , Axônios/fisiologia , Drosophila/genética , Proteínas de Drosophila , Regulação da Expressão Gênica no Desenvolvimento , Larva/citologia , Larva/genética , Morfogênese/genética , Sistema Nervoso/citologia , Neurônios/citologia , Neurônios/fisiologia
10.
J Cell Sci ; 116(Pt 15): 3213-9, 2003 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-12829740

RESUMO

Postsynaptic density protein 95 (PSD-95/SAP-90) is a palmitoylated membrane-associated guanylate kinase that oligomerizes and clusters ion channels and associated signaling machinery at excitatory synapses in brain. However, the mechanism for PSD-95 oligomerization and its relationship to ion channel clustering remain uncertain. Here, we find that multimerization of PSD-95 is determined by only its first 13 amino acids, which also have a remarkable capacity to oligomerize heterologous proteins. Multimerization does not involve a covalent linkage but rather palmitoylation of two cysteine residues in the 13 amino acid motif. This lipid-mediated oligomerization is a specific property of the PSD-95 motif, because it is not observed with other palmitoylated domains. Clustering K+ channel Kv1.4 requires interaction of palmitoylated PSD-95 with tetrameric K+ channel subunits but, surprisingly, does not require multimerization of PSD-95. Finally, disrupting palmitoylation with 2-bromopalmitate disperses PSD-95/K+-channel clusters. These data suggest new models for K+ channel clustering by PSD-95 - a reversible process regulated by protein palmitoylation.


Assuntos
Metabolismo dos Lipídeos , Proteínas do Tecido Nervoso/metabolismo , Animais , Células COS , Células Cultivadas , Chlorocebus aethiops , Clonagem Molecular , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Proteínas do Tecido Nervoso/fisiologia , Ligação Proteica/fisiologia , Estrutura Terciária de Proteína/fisiologia , Subunidades Proteicas/metabolismo , Subunidades Proteicas/fisiologia
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