Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.

Arginine (Arg), lysine (Lys), and methionine (Met) can be used to support the health status of turkeys. The present study investigated selected performance, gut integrity, and immunological parameters in turkeys reared in optimal or challenge conditions. The experiment lasted for 28 days, and it had a completely randomized 2 × 3 factorial design with two levels of dietary Arg, Lys and Met (high or low) and challenge with Clostridium perfringens (C. perfringens), Escherichia coli lipopolysaccharide (LPS) or no challenge (placebo). Increased dietary levels of Arg, Lys and Met had a beneficial effect on turkey performance and immunological parameters, and it improved selected indicators responsible for maintaining gut integrity in different challenge conditions. Under optimal conditions (with no challenge), high ArgLysMet diets did not compromise bird performance and they improved selected performance parameters in challenged birds. The immune system of turkeys was not excessively stimulated by high ArgLysMet diets, which did not disrupt the redox balance and had no negative effect on gut integrity. High ArgLysMet diets increased the expression levels of selected genes encoding nutrient transporters and tight junction proteins. However, the influence exerted by different dietary inclusion levels of Arg, Lys and Met on gut integrity was largely determined by the stressor (C. perfringens vs. LPS). Further studies are required to investigate the role of Arg, Lys and Met levels in the diet on the immune response, gut function and performance of turkeys in different challenge conditions.

Cannabidiol and Nano-Selenium Increase Microvascularization and Reduce Degenerative Changes in Superficial Breast Muscle in C. perfringens-Infected Chickens.

Here, we demonstrated the potential of Cannabis-derived cannabidiol (CBD) and nanosized selenium (nano-Se) for the modulation of microvascularization and muscle fiber lesions in superficial breast muscle in C. perfringens-challenged chickens. The administration of CBD resulted in a decreased number of atrophic fibers (3.13 vs. 1.13/1.5 mm2) compared with the control, whereas nano-Se or both substances resulted in a decreased split fiber number (4.13 vs. 1.55/1.5 mm2) and in a lower number of necrotic myofibers (2.38 vs. 0.69/1.5 mm2) in breast muscle than the positive control. There was a significantly higher number of capillary vessels in chickens in the CBD+Nano-Se group than in the control and positive control groups (1.31 vs. 0.97 and 0.98, respectively). Feeding birds experimental diets lowered the activity of DNA damage repair enzymes, including 3,N4-ethenodeoxycytosine (by 39.6%), 1,N6-ethenodeoxyadenosine (by 37.5%), 8-oxo-guanine (by 36.2%), formamidopyrimidine (fapy)-DNA glycosylase (by 56.2%) and human alkyl adenine DNA glycosylase (by 40.2%) in the ileal mucosa, but it did not compromise the blood mitochondrial oxygen consumption rate (-2.67 OD/min on average). These findings indicate a potential link between gut mucosa condition and histopathological changes in superficial pectoral muscle under induced inflammation and show the ameliorative effect of CBD and nano-Se in this cross-talk due to their protection from mucosal DNA damage.

Cannabis-derived cannabidiol and nanoselenium improve gut barrier function and affect bacterial enzyme activity in chickens subjected to C. perfringens challenge.

Revealing the multifocal mechanisms affecting cross-talk between Clostridium perfringens pathogenesis and the host response is an urgent need in the poultry industry. Herein, the activity of Cannabis sativa-derived cannabidiol (CBD) and selenium nanoparticles (Nano-Se) in modulating the host response to Clostridium perfringens challenge was investigated in broiler chickens subjected to a mild infection model. The infected chickens exhibited no clinical manifestations, confirming the potential hazard of pathogen transmission to the food chain in the commercial sector. However, both CBD and Nano-Se affected the responses of chickens to C. perfringens challenge. The beneficial actions of both agents were manifested in the upregulated expression of genes determining gut barrier function. Both CBD and Nano-Se promoted shifts in gut bacterial enzyme activity to increased energy uptake in challenged chickens and upregulated potential collagenase activity. There was no opposite effect of CBD and Nano-Se in mediating the host response to challenge, whereas an additive effect was evidenced on the upregulation of gene determining gut integrity. Collectively, these findings indicate that understanding the action mechanisms of CBD and Nano-Se is of great interest for developing a preventive strategy for C. perfringens infection in broilers.

Effects of faba bean extrusion and phytase supplementation on performance, phosphorus and nitrogen retention, and gut microbiota activity in broilers.

BACKGROUND: Faba bean (FB) can be used in poultry diets as a protein source alternative to soybean meal. This study evaluated the effect of extrusion of low-tannin FB and phytase supplementation on performance, phosphorus (P) and nitrogen (N) retention, and gut microbiota activity in broiler chickens. Raw or extruded FB meal was included in broiler diet at 300 g kg-1 . The experimental treatments additionally differed in nutrient level (adequate, or calcium, P, and protein reduced) and phytase supplementation. RESULTS: Broiler performance was not affected by the dietary treatments. Extrusion and a reduced nutrient content did not affect P and N retention coefficients, but they increased when the reduced diets were supplemented with phytase. In the ileal digesta, FB extrusion increased the activities of bacterial α- and ß-glucosidase, ß-galactosidase, and ß-glucuronidase, but acetate and total short-chain fatty acids (SCFAs) concentrations were higher only in birds fed extruded FB supplemented with phytase. In the cecal digesta, FB extrusion increased the activities of bacterial α- and ß-glucosidase, α-galactosidase and ß-glucuronidase, and phytase supplementation decreased ß-glucosidase and ß-glucuronidase activities, but acetate, butyrate and valerate and total SCFA concentrations were lower in birds fed extruded FB. CONCLUSION: Good performance of broilers could be obtained using diets containing 300 g kg-1 raw FB. The extrusion process does not improve FB nutritive value and may negatively affect the activity of cecal microbiota. Phytase supplementation allows lowering calcium, inorganic P, and protein level in diets with FB, and reduces P and N excreted in droppings, with a positive effect on the environment. © 2020 Society of Chemical Industry.

A review: Pancreatic enzymes in the treatment of chronic pancreatic insufficiency in companion animals.

The purpose of this review was to analyze the scientific literature on exocrine pancreatic insufficiency (EPI) in dogs and cats and our own research on porcine model to compare animal- and microbial-derived enzymes in the treatment of animals with this disease. Clinical signs of EPI occur when more than 85% of the pancreatic parenchyma is non-functional. EPI can be a consequence of various diseases. The insufficient activity or deficiency of pancreatic enzymes leads to impaired digestion and absorption, and consequently, to malnutrition. The primary treatment for enzyme insufficiency is pancreatic enzyme replacement therapy (PERT). PERT in animals with EPI is a lifetime therapy. Most commercially available products are of animal origin (processed pancreata obtained from a slaughter house) and contain lipases, alpha-amylase, and proteases. Enzymes of microbial and plant origin seem to be a promising alternative to animal-derived enzymes, but to date there are no registered preparations containing all enzymes simultaneously for use in clinical practice to treat EPI. Results from some previous studies have highlighted the "extra-digestive" functions of pancreatic enzymes, as well as the actions of pancreatic-like microbial enzymes. For example, trypsin activates protease-activated receptor and provokes maturation of enterocytes and enterostatin inhibits fat absorption. It has been postulated that intrapancreatic amylase is the main component of the acini-islet-acinar axis-the reflex which down regulates insulin release, while gut and blood amylase exhibit anti-incretin actions "per se." Additionally, high but still physiological blood amylase activity coincide with physiological glucose homeostasis and a lack of obesity.

Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning.

The effects of dietary probiotic supplementation with viable Bacillus subtilis and Bacillus amyloliquefaciens spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; n = 48) or a diet supplemented with viable spores (1.1 × 109 CFU/kg of feed) (probiotic; n = 48). At 7 d of age, sucking piglets (n = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (P = 0.077), improved the weaning weight (P = 0.039) and increased both the total creep feed consumption (P = 0.027) and litter gain (P = 0.011). Probiotics also improved the faecal score in the second (P = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (P = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (P = 0.050) and a lower IgG concentration in the ileal mucosa (P = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (P = 0.012) due to the presence of longer villi and larger Peyer's patches (P < 0.001). B. subtilis and B. amyloliquefaciens were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, Bacillus-based probiotic supplementation improves the health indices of sows and their piglets.

Changes in lipids metabolism indices as a result of different form of selenium supplementation in chickens.

Selenium is an essential element that is important for many metabolic processes. Feed components used in chicken nutrition, especially cereals, may be deficient in selenium, hence selenium supplementation is necessary. Taking into account the progress in breeding, and thus the higher demand of birds for this element, it seems obvious to investigate an increased selenium dose in the diet of chickens. The aim of the study was to evaluate the effect of feed enriched with different forms of selenium at an increased dose of 0.5 mg/kg feed on the profile and metabolism of fatty acids in the breast muscle and liver of chickens. The study was conducted on 300 Ross 308 chickens reared for 42 days under standard conditions. The control group received feed supplemented with sodium selenite at a dose of 0.3 mg/kg feed. The research groups received different forms of selenium (sodium selenate, selenised yeast, nano-selenium) at an increased dose of 0.5 mg/kg feed. The study showed that the administration of different forms of selenium in the feed affected its concentration in the breast muscle and liver (p ≤ 0.01). Nano-selenium was found to have a high bioavailability, but also a lower risk of toxicity compared to other forms of selenium. Using different forms of selenium (p ≤ 0.01) at a dose of 0.5 mg/kg feed can significantly modify the fatty acid profile, lipid and enzymatic indices of fatty acid metabolism in breast muscle and liver.

Bacillus-based probiotics affect gut barrier integrity in different ways in chickens subjected to optimal or challenge conditions.

Dietary supplementation with spore-forming Bacillus-based probiotics represents an efficient means to improve gut health while maintaining good broiler performance. This study investigated the potential of two probiotic products in chickens subjected to optimal (Experiment 1) and Clostridium perfringens-challenged (Experiment 2) conditions. The treatments in Experiment 1 were as follows: (i) CON (no probiotic additive), (ii) One-strain Pro (supplemented with Bacillus licheniformis) or (iii) Multi-strain Pro (supplemented with a multistrain Bacillus-based probiotic). The treatment groups in Experiment 2 received the same diets as those in Experiment 1 but were subjected to C. perfringens challenge. Both experiments lasted 35 days. Both products marginally affected broiler performance in the optimal or challenge conditions. In Experiment 1, Multi-strain Pro upregulated the mRNA expression level of 11 out of 15 selected genes, whereas in Experiment 2, this was less evident, and One-strain Pro was more effective. The multistrain probiotic was effective in maintaining gut morphostructure indices and increasing gut wall thickness, which was particularly evident in challenged birds. Neither additive induced bacterial activity (assessed by measuring enzymatic activity and short-chain fatty acid production) in the cecum, and Multi-strain Pro maintained the cecal butyrate concentration in challenged birds as in the challenged CON treatment, in which butyrate concentration was significantly higher than in the One-strain Pro treatment. Our findings indicated that the activity of these single- and multistrain probiotic products varies depending on rearing conditions, and the effect is highly strain- and product-specific. However, the multistrain probiotic apparently had more beneficial effects than the one-strain probiotic in the maintenance of gut functional status under optimal and challenge conditions.

Cannabidiol affects breast meat volatile compounds in chickens subjected to different infection models.

No study has demonstrated the use of dietary Cannabis-derived cannabidiol (CBD) to alter the stress response in chickens or examined its effects on meat volatile compounds (VOCs). Here, we subjected chickens to dysbiosis via C. perfringens infection or Escherichia coli lipopolysaccharide (LPS) treatment and investigated the potential link between meat VOCs and cecal bacterial activity and the ameliorative effect of CBD. The cecal bacterial production of short-chain fatty acids (SCFAs) was closely correlated with meat VOCs. CBD supplementation reduced the formation of breast meat spoilage VOCs, including alcohols, trimethylamine and pentanoic acid, in the challenged birds, partly by decreasing cecal putrefactive SCFA production. Meat VOC/cecal SCFA relationships differed according to the challenge, and CBD attenuated the effects of C. perfringens infection better than the effects of LPS challenge on meat VOCs. These findings provide new insights into the interactions among bioactive agent supplementation, gut microbiota activity and meat properties in birds.

Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs.

Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother's milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets.

Dietary Phytogenic Combination with Hops and a Mixture of a Free Butyrate Acidifier and Gluconic Acid Maintaining the Health Status of the Gut and Performance in Chickens.

Additives with bioactive properties can improve chickens' gut health. This study investigated the physiological status of the gut, including its morphological structure and microbiome activities in chickens fed diets supplemented with phytogenic ingredients with hops (Anta®Phyt) or a mixture of a free butyrate acidifier and gluconic acid (PreAcid). In this study, 1155 broilers were distributed to three dietary treatments with 5 replicate pens per treatment, 77 birds each. Anta®Phyt was added at 400/300/200/200 mg/kg diet whereas PreAcid was added at 3/2/1/1 g/kg starter/grower I/grower II/finisher diet respectively. Dietary treatments did not compromise body weight in different growth periods. In the birds fed PreAcid-supplemented diet, higher gut concentration of butyric acid was observed, particularly in the early stage of growth, while the profile of the short-chain fatty acids was maintained among the treatments. Neither additive significantly affected cecal bacterial enzyme activities. Feeding the birds with Anta®Phyt and PreAcid had beneficial effects on gut morphostructure indices, including intestinal wall thickness, crypt depth and the villus height to crypt depth ratio, in 35- and 42-day old birds. In conclusion, the feeding Anta®Phyt- or PreAcid-supplemented diet exerted beneficial effects on the indices determining the physiological status of the gut and maintained good performance of birds of different ages.