RESUMO
Squamous cell carcinoma (SCC) of tongue is an aggressive head and neck cancer with high propensity of regional spreading and invasion. Tongue carcinoma cells treated with curcumin, the major curcuminoid of the turmeric, demonstrated reduction in adhesion, migration, and invasion ability. High-throughput microarray analysis indicated that curcumin treatment suppressed matrix metallopeptidase 10 (MMP10) expression. MMP10 is overexpressed in tongue carcinoma tissues in comparison with the normal epithelia. Curcumin treatment on tongue carcinoma cell lines suppressed MMP10 expression at both mRNA and protein levels. Our results suggested that curcumin is a promising inhibitor to tongue cancer cells migration and invasion.
Assuntos
Antineoplásicos/farmacologia , Carcinoma de Células Escamosas/enzimologia , Carcinoma de Células Escamosas/patologia , Metaloproteinase 10 da Matriz/metabolismo , Neoplasias da Língua/enzimologia , Neoplasias da Língua/patologia , Antineoplásicos/uso terapêutico , Carcinoma de Células Escamosas/tratamento farmacológico , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Curcumina/farmacologia , Regulação para Baixo , Humanos , Neoplasias da Língua/tratamento farmacológicoRESUMO
BACKGROUND: Curcumin is a natural polyphenol. It is a potent suppressor of nuclear factor kappa B (NF-kappaB). High NF-kappaB levels have suppressive effect on E-cadherin (molecule related to cell-cell adhesion) in nasopharyngeal carcinoma (NPC) cells. We hypothesized that suppressing NF-kappaB by curcumin could up-regulate E-cadherin expression in NPC cells. MATERIALS AND METHODS: NPC cell lines HK1 and HONE1 were used. Real-time quantitative PCR and Western blotting were used to examine the expression changes of NF-kappaB and E-cadherin. A mouse xenograft model was used to validate the results. RESULTS: With curcumin treatment, NF-kappaB was down-regulated and E-cadherin was up-regulated in NPC cells. The negative correlation of NF-kappaB and E-cadherin was confirmed in the mouse xenograft model. CONCLUSION: Our results suggest that curcumin could be used in preventing NPC migration by suppressing NF-kappaB and activating E-cadherin expression.