An in vivo "turning model" reveals new RanBP9 interactions in lung macrophages.

The biological functions of the scaffold protein Ran Binding Protein 9 (RanBP9) remain elusive in macrophages or any other cell type where this protein is expressed together with its CTLH (C-terminal to LisH) complex partners. We have engineered a new mouse model, named RanBP9-TurnX, where RanBP9 fused to three copies of the HA tag (RanBP9-3xHA) can be turned into RanBP9-V5 tagged upon Cre-mediated recombination. We created this model to enable stringent biochemical studies at cell type specific level throughout the entire organism. Here, we have used this tool crossed with LysM-Cre transgenic mice to identify RanBP9 interactions in lung macrophages. We show that RanBP9-V5 and RanBP9-3xHA can be both co-immunoprecipitated with the known members of the CTLH complex from the same whole lung lysates. However, more than ninety percent of the proteins pulled down by RanBP9-V5 differ from those pulled-down by RanBP9-HA. The lung RanBP9-V5 associated proteome includes previously unknown interactions with macrophage-specific proteins as well as with players of the innate immune response, DNA damage response, metabolism, and mitochondrial function. This work provides the first lung specific RanBP9-associated interactome in physiological conditions and reveals that RanBP9 and the CTLH complex could be key regulators of macrophage bioenergetics and immune functions.

The missing spleen - A diagnosis of medical identity fraud in surgery: Case report.

INTRODUCTION AND IMPORTANCE: Medical identity fraud is a growing concern in surgery and can adversely affect patient care. Fraudulent medical information can result in misdiagnoses or inadequate preoperative workup of surgical patients. CASE PRESENTATION: A 63-year-old female presented to hospital with a fishbone-related oesophageal perforation. Unbeknownst to the surgical team, the patient was committing medical identity fraud and impersonating another 57-year-old female. She underwent a gastroscopy with removal of the fishbone while her true identity remained concealed. A progress CT scan performed three days post-gastroscopy revealed an absent spleen, which was present on ultrasound as recent as three weeks prior to her admission. The patient was confronted about this discrepancy and confessed to committing medical identity fraud. CLINICAL DISCUSSION: Detection of medical identity fraud remains a challenging problem in healthcare systems worldwide. Various methods have been proposed to improve detection of medical identity fraud but there has been limited studies on their effectiveness in hospital settings. Given the serious health repercussions that can arise from medical identity fraud, it is imperative that better detection methods become available in the future. CONCLUSION: Medical identity fraud is a complex problem that requires better detection methods to prevent patient harm. Surgeons should be aware of the adverse health consequences that can arise from medical identity fraud.