Multielectrode bisensor system for time-resolved monitoring of ion transport across an epithelial cell layer.

An ion-selective multielectrode bisensor system is designed to ensure reliable real-time concentration measurements of sodium, potassium, chloride, and pH in a small volume of biological liquid bathing a living human bronchial epithelial cell monolayer. The bisensor system allows the monitoring of major ions, which are simultaneously transported through the epithelia in both directions.

Ion transporting proteins of human bronchial epithelium.

The electrolyte transport system across human airway epithelium followed by water movement is essential for the normal mucociliary clearance that allows the maintenance of the aseptic condition of the respiratory tract. The function of epithelial cells is to control and regulate ionic composition and volume of fluids in the airways. Various types of proteins taking part in assuring effective ions and water transport in apical and basolateral membranes of the airway epithelium have been found (e.g., CFTR, ENaC, CaCC, ORCC, potassium channels, NaKATPase, aquaporins). The paper reviews the current state of the art in the field of ion channels, transporters, and other signaling proteins identified in the human bronchial epithelium.

Discharge Plasma Treatment as an Efficient Tool for Improved Poly(lactide) Adhesive-Wood Interactions.

Poly(lactide) (PLA) films obtained by thermoforming or solution-casting were modified by diffuse coplanar surface barrier discharge plasma (300 W and 60 s). PLA films were used as hot-melt adhesive in joints in oak wood. It was demonstrated that lap shear strength increased from 3.4 to 8.2 MPa, respectively, for the untreated and plasma-treated series. Pull-off tests performed on particleboard for the untreated and treated PLA films showed 100% cohesive failure. Pull-off strength tests on solid oak demonstrated adhesion enhancement from 3.3 MPa with the adhesion failure mode to 6.6 MPa with the cohesion failure mode for untreated and treated PLA. XPS revealed that carbonyl oxygen content increased by two-to-three-fold, which was confirmed in the Fourier-transform infrared spectroscopy experiments of the treated PLA. The water contact angle decreased from 66.4° for the pristine PLA to 49.8° after treatment. Subsequently, the surface free energy increased from 47.9 to 61.05 mJ/m2. Thus, it was clearly proven that discharge air plasma can be an efficient tool to change surface properties and to strengthen adhesive interactions between PLA and woody substrates.

Wood-Based Panel Industry Wastewater Meets Microbial Fuel Cell Technology.

Although the wood-based panel industry is not considered to be a water-consuming sector, it generates ca. 600 M m3 of wastewater every year on a global scale. The wastewater is usually highly polluted and environmentally toxic even after dilution. Common wastewater treatment techniques require high-energy input or addition of various chemicals to the treated wastewater, which cause secondary pollution and production of toxic sludge. Microbial fuel cells (MFCs) have become an attractive technology, allowing for zero-energy treatment of various types of wastewater with simultaneous production of electric current. Recent investigations have shown that MFCs can also be utilized for sustainable treatment and energy production from the wastewater generated by the wood-based panel industry. This article contains a critical summary of the investigations in this field as well as a discussion of the research needed and perspectives for the future.

Bioelectricity production from wood hydrothermal-treatment wastewater: Enhanced power generation in MFC-fed mixed wastewaters.

Electrogenic microorganisms are the heart of microbial fuel cell (MFC) systems that enable the conversion of waste into bioelectricity. Bacteria able to generate current, found in various natural and anthropogenic environments, need simple substrates such as acetate or glucose. Complex substrates are utilized by bacterial consortia made up of strains that exhibit a wide range of enzymatic and metabolic activity that determines the type of substrate they are able to degrade. The characteristics of the environment that a bacterial consortium develops in strongly affect the consortium's species composition and electrogenic potential. This study presents the first attempt to use industrial raw wastewater from the hydrothermal treatment of wood (WHTW) as a substrate and a source of bacterial consortia for MFC, so that such wastewater could simultaneously be treated and produce bioelectricity. The power generated in MFCs fed with WHTW was enhanced remarkably from 70 to 360mW/m2 when municipal wastewater was introduced into the reactor. An analysis of the bacterial composition of these two types of wastewater revealed that the WHTW was dominated by the genera Thermoanaerobacterium and Paenibacillus while in the biofilm developed in the anode the main genera were Hydrogenophilus and Anaerobaculum. It has been shown for the first time that highly polluted wood industry wastewater may be effectively treated in MFC systems and the use of appropriate bacterial consortium may result in enhancing power generation accompanying wastewater treatment.

Design and characterization of novel all-solid-state potentiometric sensor array dedicated to physiological measurements.

A novel construction of all-solid-state potentiometric sensor array designed for physiological measurements has been presented. The planar construction and elimination of liquid phase creates broad opportunities for the modifications in the sensing part of the sensor. The designed construction is based on all-solid-state ion-selective electrodes integrated with the ionic-liquid based reference electrode. Work parameters of the sensor arrays were characterized. It has been shown that presented sensor design indicates high sensitivity (55.2±1mV/dec, 56.3±2mV/dec, 58.4±1mV/dec and 53.5±1mV/pH for sodium-, potassium-, chloride- and pH-selective electrodes, respectively in 10(-5)-10(-1.5)M range of primary ions), low response time (t95 did not exceed 10s), high potential stability (potential drift in 28-h measurement was ca. ±2mV) and potential repeatability ca. ±1mV. The system was successfully applied to the simultaneous determination of K(+), Cl(-), Na(+) and pH in the model physiological solution and for the ion flux studies in human colon epithelium Caco-2 cell line as well.

Potassium channel in the mitochondria of human keratinocytes.

The activation of mitochondrial potassium channels induces cytoprotection in various cell types. Hence, the identification of ion channels present in the inner mitochondrial membrane of keratinocytes is important in distinguishing possible protective mechanisms in these cells. In this paper, inner membrane mitochondrial ion channels of the human keratinocyte HaCaT cell line were investigated using a patch-clamp technique. We observed potassium-selective channel activity with a conductance of 83 pS at positive voltages. The I-V curve indicates that the observed channel has rectifying properties. Moreover, the channel activity was inhibited by acidic pH and 1 mM lidocaine. Using reverse transcriptase-PCR, we found an mRNA transcript for the TASK-3 (tandem pore domain acid-sensitive K channels) channel. We observed co-localization of the TASK-3 protein and a mitochondrial marker in the mitochondria of HaCaT cells. Additionally, we showed that TASK-3 knockdown HaCaT cells markedly decreased viability after UVB radiation exposure compared with control cells. In summary, the single-channel activity and properties of a mitochondrial potassium channel in a keratinocyte HaCaT cell line have been described.