RESUMO
This study probed in vitro the mechanisms of competition/coexistence between Streptococcus sanguinis (known for being correlated with health in the oral cavity) and Streptococcus mutans (responsible for aciduric oral environment and formation of caries) by means of quantitative Raman spectroscopy and imaging. In situ Raman assessments of live bacterial culture/coculture focusing on biofilm exopolysaccharides supported the hypothesis that both species engaged in antagonistic interactions. Experiments of simultaneous colonization always resulted in coexistence, but they also revealed fundamental alterations of the biofilm with respect to their water-insoluble glucan structure. Raman spectra (collected at fixed time but different bacterial ratios) showed clear changes in chemical bonds in glucans, which pointed to an action by Streptococcus sanguinis to discontinue the impermeability of the biofilm constructed by Streptococcus mutans. The concurrent effects of glycosidic bond cleavage in water-insoluble α - 1,3-glucan and oxidation at various sites in glucans' molecular chains supported the hypothesis that secretion of oxygen radicals was the main "chemical weapon" used by Streptococcus sanguinis in coculture.
Assuntos
Cárie Dentária , Streptococcus sanguis , Humanos , Streptococcus mutans , Biofilmes , Boca/microbiologia , Glucanos/farmacologiaRESUMO
α-1,6-Graft-α-1,3-glucan comprises a main-chain of α-1,6-glucan and side-chains of α-1,3-glucan. It was synthesized by a one-pot in vitro enzymatic polymerization of sucrose and dextran (α-1,6-glucan) of different molecular weights. In the presence of the high-molecular-weightdextran (Mw ≥ 650â¯000), the graft glucan formed a self-standing hydrogel without any cross-linker. It was possible to control the number of α-1,3-glucan side-chains by controlling the molecular weight and concentration of the dextran. Consequently, it was possible to control the compression strength of the obtained gels. Hydrogels of the graft glucan were formed by physically cross-linking the α-1,3-glucan side-chains. These physical gels are potentially useful biomaterials with high biocompatible, because the graft glucan is composed of glucose alone.
Assuntos
Glucanos , Hidrogéis , Peso Molecular , PolimerizaçãoRESUMO
In this study, a new system was developed to carry out simultaneous near-infrared (NIR) and small-angle X-ray scattering (SAXS) measurements. Aged PP was examined with the NIR-SAXS system to demonstrate how it can be utilized to derive pertinent information about polymer structure. Pairs of SAXS profiles and NIR spectra of PP in its initial state and after aging were measured to derive an in-depth understanding of the aging phenomenon. The SAXS profiles of the PP samples showed a clear shift of the SAXS peak to the lower q direction induced by the thermal aging, indicating an increase in the length of the long-period structure. Two-trace two-dimensional (2T2D) asynchronous correlation spectra derived from NIR spectra clearly revealed that the aging treatment leads to the substantial increase in the spectral intensity of the regularity bands representing the longer helix present in a folded lamellar structure. In other words, it suggest that the long helix structure is more abundantly present than the short helix structure in the aged PP than in the initial PP. By combining the information derived from the SAXS profiles and NIR spectra, the details of the aging-induced variation were clearly determined. Namely, aging causes additional crystallization of the PP by developing more helix structures, which involves an increase in lamellar thickness as well as a decrease in the amorphous region. The growth of the rigid crystalline phase restricts the elastic deformation in the amorphous structure, which eventually induces the deterioration of PP by making the polymer hard but brittle. Such observation, in turn, implies that the retarding or accelerating the crystallized structure of PP substantially works to control the progress of the aging.
RESUMO
α-1,3-Glucan is a linear and crystalline polysaccharide which is synthesized by in vitro enzymatic polymerization from sucrose. A previous study reported that regenerated fibers of α-1,3-glucan were prepared using a wet-spinning method. However, the mechanical properties were poorer than cellulose regenerated fibers. Then, in this study, the mechanical properties of the regenerated α-1,3-glucan fiber were improved by the transformation of the crystal structure and stretching. The regenerated fiber stretched in water and dehydrated by heating showed high tensile strength (18 cN/tex) that is comparable with that of viscose rayon. Moreover, the crystal structures of the regenerated fibers were investigated using wide-angle X-ray diffraction (WAXD). To date, four crystal polymorphs of α-1,3-glucan from polymorph I to IV have been reported. This study revealed that the regenerated α-1,3-glucan fibers had two different polymorphs, polymorph II (hydrated form) and polymorph III (anhydrous form), depending on post-treatment methods of stretching and annealing procedures. Furthermore, the obtained distinctive 2D-WAXD patterns suggested that polymorph III is identical to polymorph IV.