Effects of single and dual-stressor elevation of environmental temperature and PCO2 on metabolism and acid-base regulation in the Louisiana red swamp crayfish, Procambarus clarkii.

Elevation of temperature and CO2 levels within the world's aquatic environments is expected to cause numerous physiological challenges to their inhabitants. While effects on marine ecosystems have been well studied, freshwater ecosystems have rarely been examined using a dual-stressor approach leaving our understanding of its inhabitants upon these challenges unclear. We aimed to identify the affects of elevated temperature and hypercapnia in isolation and in combination on the metabolic and acid-base regulatory processes of a freshwater crayfish, Procambarus clarkii. Crayfish were exposed to freshwater conditions that may be prevalent by the year 2100 and metabolic responses were determined after 14-days of exposure. In addition, changes in branchial mRNA expression of acid-base linked transporters were investigated. Interactions between exposure conditions influenced extracellular pH as well as the nitrogen physiology and routine metabolic rate of the crayfish. Crayfish exposed to individual and combined elevations in temperature and/or hypercapnia maintained an extracellular pH similar to that of control crayfish. Dual-stressor exposed crayfish seem to elevate the importance of ammonium as an excretable acid-equivalent based on an overall increase in the branchial mRNA expression of transporters related to ammonia excretion including the Na+/K+-ATPase, Rhesus-protein, and the V-type H+-ATPase. Overall, hypercapnia and dual-stressor conditions caused a metabolic depression that may have long-lasting consequences such as limited locomotion, growth, and reproduction. Future generations of crayfish given the chance to adapt over several generations may ameliorate these consequences.

Impact of climate change on the American lobster (Homarus americanus): Physiological responses to combined exposure of elevated temperature and pCO2.

The physiological consequences of exposing marine organisms to predicted future ocean scenarios, i.e. simultaneous increase in temperature and pCO2, have only recently begun to be investigated. Adult American lobster (Homarus americanus) were exposed to either current (16 °C, 47 Pa pCO2, pH 8.10) or predicted year 2300 (20 °C, 948 Pa pCO2, pH 7.10) ocean parameters for 14-16 days prior to assessing physiological changes in their hemolymph parameters as well as whole animal ammonia excretion and resting metabolic rate. Acclimation of lobster simultaneously to elevated pCO2 and temperature induced a prolonged respiratory acidosis that was only partially compensated for via accumulation of extracellular HCO3- and ammonia. Furthermore, acclimated animals possessed significantly higher ammonia excretion and oxygen consumption rates suggesting that future ocean scenarios may increase basal energetic demands on H. americanus. Enzyme activity related to protein metabolism (glutamine dehydrogenase, alanine aminotransferase, and aspartate aminotransferase) in hepatopancreas and muscle tissue were unaltered in future ocean scenario exposed animals; however, muscular citrate synthase activity was reduced suggesting that, while protein catabolism may be unchanged, the net energetic output of muscle may be compromised in future scenarios. Overall, H. americanus acclimated to ocean conditions predicted for the year 2300 appear to be incapable of fully compensating against climate change-related acid-base challenges and experience an increase in metabolic waste excretion and oxygen consumption. Combining our study with past literature on H. americanus suggests that the whole lifecycle from larvae to adult stages is at risk of severe growth, survival and reproductive consequences due to climate change.