1.
Biotechniques
; 46(3 Suppl): Piii-Pviii, 2009 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19317668
RESUMO
The design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. This system is appropriate for low biohazard-level agents. In this study, two biosafety level 2 agents, Pasturella pneumotropica and Pseudomonas aeruginosa, were tested successfully. This system was also used to immunize mice and guinea pigs in ovalbumin-based models of pulmonary inflammation. This design is appropriate for studies with limited budgets and lower-level biosafety containment.