RESUMO
Butterfly wing scales containing photonic nanoarchitectures act as chemically selective sensors due to their color change when mixing vapors in the atmosphere. Based on butterfly vision, we built a model for efficient characterization of the spectral changes in different atmospheres. The spectral shift is vapor specific and proportional with the vapor concentration. Results were compared to standard principal component analysis. The modification of the chemical properties of the scale surface by the deposition of 5 nm of Al(2)O(3) significantly alters the character of the optical response. This is proof of the possibility to purposefully tune the selectivity of such sensors.
Assuntos
Borboletas/fisiologia , Gases/análise , Fótons , Asas de Animais/química , Animais , Microscopia Eletrônica de Varredura , PigmentaçãoRESUMO
The effects produced by the condensation of water vapor from the environment in the various intricate nanoarchitectures occurring in the wing scales of several Lepidoptera species were investigated by controlled cooling (from 23° C, room temperature to -5 to -10° C) combined with in situ measurements of changes in the reflectance spectra. It was determined that all photonic nanoarchitectures giving a reflectance maximum in the visible range and having an open nanostructure exhibited alteration of the position of the reflectance maximum associated with the photonic nanoarchitectures. The photonic nanoarchitectures with a closed structure exhibited little to no alteration in color. Similarly, control specimens colored by pigments did not exhibit a color change under the same conditions. Hence, this method can be used to identify species with open photonic nanoarchitectures in their scales. For certain species, an almost complete disappearance of the reflectance maximum was found. All specimens recovered their original colors following warming and drying. Cooling experiments using thin copper wires demonstrated that color alterations could be limited to a width of a millimeter or less. Dried museum specimens did not exhibit color changes when cooled in the absence of a heat sink due to the low heat capacity of the wings.
Assuntos
Cor , Entomologia/métodos , Lepidópteros/química , Óptica e Fotônica/métodos , Asas de Animais/química , Animais , Temperatura Baixa , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de TransmissãoRESUMO
The male Troides magellanus--a birdwing butterfly that lives in a restricted area of the Philippines--concentrates on its hindwings at least two distinct optical processes that contribute to its exceptional visual attraction. The first is the very bright uniform yellow coloration caused by a pigment which generates yellow-green fluorescence, and the other is a blue-green iridescence which results from light diffraction at grazing emergence under a specific illumination. Detailed optical measurements reveal that these optical effects are correlated, the fluorescence being enhanced by illuminations conditions that favor the occurrence of the iridescence. These effects are analyzed, with the conclusion that both of them depend on the same optical device: a one-dimensional microribs grating which appear on the sides of the ridges that run along the yellow scales.
Assuntos
Borboletas/fisiologia , Asas de Animais/fisiologia , Animais , Borboletas/anatomia & histologia , Fluorescência , Masculino , Dispositivos Ópticos , Pigmentação/fisiologia , Refratometria/métodos , Asas de Animais/anatomia & histologiaRESUMO
The tortoise beetle Charidotella egregia is able to modify the structural color of its cuticle reversibly, when disturbed by stressful external events. After field observations, measurements of the optical properties in the two main stable color states and scanning electron microscope and transmission electron microscope investigations, a physical mechanism is proposed to explain the color switching of this insect. It is shown that the gold coloration displayed by animals at rest arises from a chirped multilayer reflector maintained in a perfect coherent state by the presence of humidity in the porous patches within each layer, while the red color displayed by disturbed animals results from the destruction of this reflector by the expulsion of the liquid from the porous patches, turning the multilayer into a translucent slab that leaves an unobstructed view of the deeper-lying, pigmented red substrate. This mechanism not only explains the change of hue but also the change of scattering mode from specular to diffuse. Quantitative modeling is developed in support of this analysis.
Assuntos
Besouros/anatomia & histologia , Pigmentação , Animais , Umidade , Luz , Espectrometria de Massas , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Porosidade , Refratometria , Espalhamento de RadiaçãoRESUMO
Photonic-crystal-type nanostructures occurring in the scales of the butterfly Cyanophrys remus were investigated by optical and electron microscopy (scanning and transmission electron microscopy), reflectance measurements (specular, integrated, and goniometric), by fast Fourier transform analysis of micrographs, by modeling, and by numerical simulation of the measured reflectance data. By evaluating the collected data in a cross-correlated way, we show that the metallic blue dorsal coloration originates from scales which individually are photonic single crystals of 50 x 120 microm2 , while the matt pea-green coloration of the ventral side arises from the cumulative effect of randomly arranged, bright photonic crystallites (blue, green, and yellow) with typical diameters in the 3-10-mum range. Both structures are based on a very moderate refractive index contrast between air and chitin. Using a bleached specimen in which the pigment has decayed with time, we investigated the role of pigment in photonic-crystal material in the process of color generation. The possible biologic utility of the metallic blue (single-crystal) and dull green (polycrystal) textures both achieved with photonic crystals are briefly discussed. Potential applications in the field of colorants, flat panel displays, smart textiles, and smart papers are surveyed.
Assuntos
Borboletas/química , Borboletas/fisiologia , Cristais Líquidos/química , Modelos Biológicos , Modelos Químicos , Asas de Animais/química , Asas de Animais/fisiologia , Animais , Cor , Simulação por Computador , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Refratometria , Propriedades de SuperfícieRESUMO
The optical properties of the inflorescence of the high-altitude Leontopodium nivale subsp. alpinum (edelweiss) is investigated, in relation with its submicrometer structure, as determined by scanning electron microscopy. The filaments forming the hair layer have been found to exhibit an internal structure which may be one of the few examples of a photonic structure found in a plant. Measurements of light transmission through a self-supported layer of hair pads taken from the bracts supports the idea that the wooly layer covering the plant absorbs near-ultraviolet radiation before it reaches the cellular tissue. Calculations based on a photonic-crystal model provide insight on the way radiation can be absorbed by the filamentary threads.
Assuntos
Asteraceae/citologia , Asteraceae/fisiologia , Cabelo/citologia , Cabelo/fisiologia , Modelos Biológicos , Refratometria/métodos , Asteraceae/efeitos da radiação , Simulação por Computador , Cabelo/efeitos da radiação , Luz , Relação Estrutura-AtividadeRESUMO
The photonic nanoarchitectures responsible for the blue colour of the males of nine polyommatine butterfly species living in the same site were investigated structurally by electron microscopy and spectrally by reflectance spectroscopy. Optical characterization was carried out on 110 exemplars. The structural data extracted by dedicated software and the spectral data extracted by standard software were inputted into an artificial neural network software to test the specificity of the structural and optical characteristics. It was found that both the structural and the spectral data allow species identification with an accuracy better than 90 per cent. The reflectance data were further analysed using a colour representation diagram built in a manner analogous to that of the human Commission Internationale de l'Eclairage diagram, but the additional blue visual pigment of lycaenid butterflies was taken into account. It was found that this butterfly-specific colour representation diagram yielded a much clearer distinction of the position of the investigated species compared with previous calculations using the human colour space. The specific colours of the investigated species were correlated with the 285 flight-period data points extracted from museum collections. The species with somewhat similar colours fly in distinct periods of the year such that the blue colours are well tuned for safe mate/competitor recognition. This allows for the creation of an effective pre-zygotic isolation mechanism for closely related synchronic and syntopic species.
Assuntos
Comunicação Animal , Lepidópteros/fisiologia , Lepidópteros/ultraestrutura , Pigmentação/efeitos da radiação , Asas de Animais/fisiologia , Asas de Animais/ultraestrutura , Animais , Masculino , Redes Neurais de Computação , Análise EspectralRESUMO
The self-assembled photonic nanoarchitectures occurring in the wing scales of the blue colored males of nine Lycaenid butterfly species, living in the same habitat, were investigated by reflectance measurements followed by automated data processing. The spectral signatures of the nine species analyzed using an artificial neural network software show that despite the fact that all possess similar "pepper pot" type structure, the spectral signatures exhibit enough characteristic differences to allow the unambiguous identification of conspecific individuals. By cross-correlating the position of the individuals of each species in the CIE chromaticity diagram with their flying period it was possible to show that relatively similarly looking, closely related species fly in distinct periods. The spectral identification method may prove useful in the investigation of museum exemplars which cannot be harmed. As the self-assembled, quasiordered, "pepper pot" type photonic nanoarchitectures of various colors seem to pose milder constraints on the production process as compared with perfect photonic crystals, such nanoarchitectures may find practical applications in a wide range from the textile industry to environmentally friendly colorants.
RESUMO
In order to study local and global order in butterfly wing scales possessing structural colors, we have developed a direct space algorithm, based on averaging the local environment of the repetitive units building up the structure. The method provides the statistical distribution of the local environments, including the histogram of the nearest-neighbor distance and the number of nearest neighbors. We have analyzed how the different kinds of randomness present in the direct space structure influence the reciprocal space structure. It was found that the Fourier method is useful in the case of a structure randomly deviating from an ordered lattice. The direct space averaging method remains applicable even for structures lacking long-range order. Based on the first Born approximation, a link is established between the reciprocal space image and the optical reflectance spectrum. Results calculated within this framework agree well with measured reflectance spectra because of the small width and moderate refractive index contrast of butterfly scales. By the analysis of the wing scales of Cyanophrys remus and Albulina metallica butterflies, we tested the methods for structures having long-range order, medium-range order, and short-range order.