RESUMO
BACKGROUND: Systematic reviews allow health decisions to be informed by the best available research evidence. However, their number is proliferating quickly, and many skills are required to identify all the relevant reviews for a specific question. METHODS AND FINDINGS: We screen 10 bibliographic databases on a daily or weekly basis, to identify systematic reviews relevant for health decision-making. Using a machine-based approach developed for this project we select reviews, which are then validated by a network of more than 1000 collaborators. After screening over 1,400,000 records we have identified more than 300,000 systematic reviews, which are now stored in a single place and accessible through an easy-to-use search engine. This makes Epistemonikos the largest database of its kind. CONCLUSIONS: Using a systematic approach, recruiting a broad network of collaborators and implementing automated methods, we developed a one-stop shop for systematic reviews relevant for health decision making.
Assuntos
Atenção à Saúde , Ferramenta de Busca , Bases de Dados Bibliográficas , Bases de Dados Factuais , Humanos , Revisões Sistemáticas como AssuntoRESUMO
Age is the main risk factor for the development of neurodegenerative diseases. In the aged brain, axonal degeneration is an early pathological event, preceding neuronal dysfunction, and cognitive disabilities in humans, primates, rodents, and invertebrates. Necroptosis mediates degeneration of injured axons, but whether necroptosis triggers neurodegeneration and cognitive impairment along aging is unknown. Here, we show that the loss of the necroptotic effector Mlkl was sufficient to delay age-associated axonal degeneration and neuroinflammation, protecting against decreased synaptic transmission and memory decline in aged mice. Moreover, short-term pharmacologic inhibition of necroptosis targeting RIPK3 in aged mice, reverted structural and functional hippocampal impairment, both at the electrophysiological and behavioral level. Finally, a quantitative proteomic analysis revealed that necroptosis inhibition leads to an overall improvement of the aged hippocampal proteome, including a subclass of molecular biofunctions associated with brain rejuvenation, such as long-term potentiation and synaptic plasticity. Our results demonstrate that necroptosis contributes to age-dependent brain degeneration, disturbing hippocampal neuronal connectivity, and cognitive function. Therefore, necroptosis inhibition constitutes a potential geroprotective strategy to treat age-related disabilities associated with memory impairment and cognitive decline.
Assuntos
Necroptose , Doenças Neurodegenerativas , Humanos , Camundongos , Animais , Idoso , Proteômica , Rejuvenescimento , Envelhecimento/fisiologia , Encéfalo , Transtornos da MemóriaRESUMO
Se encontraron metacercarias de tres especies nuevas de Tylodelphys (Trematoda, Dipostomatidae), en el cerebro de Galaxias maculatus (Teleostei, Galaxiidae) del Lago Meliquina, Provincia de Neuquén, Argentina. Estas metacercarias se describen morfológicamente bajo los nombres de Tylodelphys argentinus n. sp., Tylodelphys barilochensis n. sp. y Tylodelphys crubensis n. sp. Esta es la primera cita de un estadio de Tylodelphys parasitando a Galaxias maculatus. Metacercarias de T. barilochensis se hallaron también en el cerebro de G. maculatus del Lago Perito Moreno, Provincia de Río Negro, Argentina
Assuntos
Animais , Peixes/parasitologia , Trematódeos/classificação , Argentina , Encéfalo/parasitologia , Larva/parasitologia , Prevalência , Trematódeos/isolamento & purificaçãoRESUMO
Chilina sp., abundante en las aguas dulces patagónicas, es un hospedador intermediario en el ciclo biológico de varios trematodes digeneos. En la masa visceral de ejemplares de distintas procedencias, se encontraron larvas de digeneos en diferentes estadíos, que se determinaron a nivel de familia. Se dan ha conocer estos hallazgos, con datos de presencia y prevalencia, como contribución al conocimiento parasitológico de los ambientes estudiados