Resource availability and disturbance frequency shape evolution of plant life forms in Neotropical habitats.

Organisms use diverse strategies to thrive in varying habitats. While life history theory partly explains these relationships, the combined impact of resource availability and disturbance frequency on life form strategy evolution has received limited attention. We use Chamaecrista species, a legume plant lineage with a high diversity of plant life forms in the Neotropics, and employ ecological niche modeling and comparative phylogenetic methods to examine the correlated evolution of plant life forms and environmental niches. Chamaephytes and phanerophytes have optima in environments characterized by moderate water and nutrient availability coupled with infrequent fire disturbances. By contrast, annual plants thrive in environments with scarce water and nutrients, alongside frequent fire disturbances. Similarly, geophyte species also show increased resistance to frequent fire disturbances, although they thrive in resource-rich environments. Our findings shed light on the evolution of plant strategies along environmental gradients, highlighting that annuals and geophytes respond differently to high incidences of fire disturbances, with one enduring it as seeds in a resource-limited habitat and the other relying on reserves and root resprouting systems in resource-abundant habitats. Furthermore, it deepens our understanding of how organisms evolve associated with their habitats, emphasizing a constraint posed by low-resource and high-disturbance environments.

Investigating historical drivers of latitudinal gradients in polyploid plant biogeography: A multiclade perspective.

PREMISE: The proportion of polyploid plants in a community increases with latitude, and different hypotheses have been proposed about which factors drive this pattern. Here, we aimed to understand the historical causes of the latitudinal polyploidy gradient using a combination of ancestral state reconstruction methods. Specifically, we assessed whether (1) polyploidization enables movement to higher latitudes (i.e., polyploidization precedes occurrences in higher latitudes) or (2) higher latitudes facilitate polyploidization (i.e., occurrence in higher latitudes precedes polyploidization). METHODS: We reconstructed the ploidy states and ancestral niches of 1032 angiosperm species at four paleoclimatic time slices ranging from 3.3 million years ago to the present, comprising taxa from four well-represented clades: Onagraceae, Primulaceae, Solanum (Solanaceae), and Pooideae (Poaceae). We used ancestral niche reconstruction models alongside a customized discrete character evolution model to allow reconstruction of states at specific time slices. Patterns of latitudinal movement were reconstructed and compared in relation to inferred ploidy shifts. RESULTS: No single hypothesis applied equally well across all analyzed clades. While significant differences in median latitudinal occurrence were detected in the largest clade, Poaceae, no significant differences were detected in latitudinal movement in any clade. CONCLUSIONS: Our preliminary study is the first to attempt to connect ploidy changes to continuous latitudinal movement, but we cannot favor one hypothesis over another. Given that patterns seem to be clade-specific, more clades must be analyzed in future studies for generalities to be drawn.

Towards a species-level phylogeny for Neotropical Myrtaceae: Notes on topology and resources for future studies.

PREMISE: Increasingly complete phylogenies underpin studies in systematics, ecology, and evolution. Myrteae (Myrtaceae), with ~2700 species, is a key component of the exceptionally diverse Neotropical flora, but given its complicated taxonomy, automated assembling of molecular supermatrices from public databases often lead to unreliable topologies due to poor species identification. METHODS: Here, we build a taxonomically verified molecular supermatrix of Neotropical Myrteae by assembling 3909 published and 1004 unpublished sequences from two nuclear and seven plastid molecular markers. We infer a time-calibrated phylogenetic tree that covers 712 species of Myrteae (~28% of the total diversity in the clade) and evaluate geographic and taxonomic gaps in sampling. RESULTS: The tree inferred from the fully concatenated matrix mostly reflects the topology of the plastid data set and there is a moderate to strong incongruence between trees inferred from nuclear and plastid partitions. Large, species-rich genera are still the poorest sampled within the group. Eastern South America is the best-represented area in proportion to its species diversity, while Western Amazon, Mesoamerica, and the Caribbean are the least represented. CONCLUSIONS: We provide a time-calibrated tree that can be more reliably used to address finer-scale eco-evolutionary questions that involve this group in the Neotropics. Gaps to be filled by future studies include improving representation of taxa and areas that remain poorly sampled, investigating causes of conflict between nuclear and plastid partitions, and the role of hybridization and incomplete lineage sorting in relationships that are poorly supported.

An assessment of methods to combine evolutionary history and conservation: A case study in the Brazilian campo rupestre.

Premise: Conservation policies typically focus on biodiversity hotspots. An alternative approach involves analyzing the evolutionary history of lineages in geographic areas along with their threat levels to guide conservation efforts. Mountains exhibit high levels of plant species richness and micro-endemism, and biogeographic studies commonly point to recent and rapid evolutionary radiations in these areas. Using a nearly endemic clade of legumes, our study evaluates conservation prioritization approaches in the campo rupestre, a Neotropical ecosystem associated with mountaintops that is located between two biodiversity hotspots. Methods: We compared the EDGE and EDGE2 metrics, which combine the evolutionary distinctiveness and the extinction risk of a species in a single value. These metrics are compared with traditional metrics used to assess conservation priority, such as phylogenetic diversity. Results: The EDGE values reported are lower than those of other studies using this metric, mostly due to the prevalence of threatened species with short phylogenetic branch lengths (low values of evolutionary distinctiveness). Certain areas of campo rupestre with relatively high phylogenetic diversity and EDGE values do not correspond to areas with high species richness, agreeing with previous studies on biodiversity hotspots. Discussion: Our study highlights the necessity of conservation of the campo rupestres as well as advantages and disadvantages of using EDGE, EDGE2, and phylogenetic diversity for appropriate selection of conservation areas with rapid evolutionary radiations. The selection of the metrics will depend primarily on the life history of the focus group and the data availability, as well as the conservation approach.

How many meals and snacks do Brazilians eat a day? Findings from the 2017-2018 Brazilian National Dietary Survey.

The habit of eating specific meals has been addressed in several studies, but the evaluation of meal patterns has received less attention. This study aimed to describe the meal patterns of the Brazilian population. A complex sampling design was used to select the 46,164 ≥ 10-year-old individuals examined in the Brazilian National Dietary Survey. Food consumption was assessed by two non-consecutive 24-hour recalls applied throughout a one-week period. The exploratory data analysis approach was used to determine the meal patterns, i.e., how individuals combined the main meals (breakfast, lunch, dinner) and snacks (morning, afternoon, evening/night) throughout the day. The most common meal patterns were three main meals + one snack, reported by 25.1% of the individuals, and three main meals + two snacks (24.6%). Other meal patterns identified were: three main meals + three snacks (18.5%); three main meals and no snacks (10.9%); one or two main meals + two snacks (7.4%); one or two main meals + one snack (6.9%); one or two main meals + three snacks (4.2%); and one or two main meals and no snacks (2.3%). Meal patterns varied according to gender and age group, and on typical versus atypical food consumption days. We found that eight patterns characterized the daily meal consumption in Brazil. Furthermore, around 80% of the population had three main meals every day and about 13% did not report having any snacks. The characterization of meal habits is important for tailoring and targeting health promotion actions.

High-risk clones of carbapenem resistant Klebsiella pneumoniae recovered from pediatric patients in Southern Brazil.

Carbapenem-resistant Klebsiella pneumoniae (CRKP) exhibit high mortality rates in pediatric patients and usually belong to international high-risk clones. This study aimed to investigate the molecular epidemiology and carbapenem resistance mechanisms of K. pneumoniae isolates recovered from pediatric patients, and correlate them with phenotypical data. Twenty-five CRKP isolates were identified, and antimicrobial susceptibility was assessed using broth microdilution. Carbapenemase production and ß-lactamase genes were detected by phenotypic and genotypic tests. Multilocus sequence typing was performed to differentiate the strains and whole-genome sequencing was assessed to characterize a new sequence type. Admission to the intensive care unit and the use of catheters were significantly positive correlates of CRKP infection, and the mortality rate was 36%. Almost all isolates showed multidrug-resistant phenotype, and most frequent resistant gene was blaKPC. We observed the dissemination of ST307 and clones belonging to CG258, which are considered high risk. In pediatric patients, these clones present with high genomic plasticity, favoring adaptation of the KPC and NDM enzymes to healthcare environments.

Long COVID: neurological manifestations - an updated narrative review.

Infection with the SARS-CoV-2 virus can lead to neurological symptoms in the acute phase and in the Long COVID phase. These symptoms usually involve cognition, sleep, smell disorders, psychiatric manifestations, headache and others. This condition is more commonly described in young adults and women. This symptomatology can follow severe or mild cases of the disease. The importance of this issue resides in the high prevalence of neurological symptoms in the Long COVID phase, which entails significant morbidity in this population. In addition, such a condition is associated with high health care costs, with some estimates hovering around 3.7 trillion US dollars. In this review, we will sequentially describe the current knowledge about the most prevalent neurological symptoms in Long COVID, as well as their pathophysiology and possible biomarkers.

A infecção pelo vírus SARS-CoV-2 pode levar a sintomas neurológicos na fase aguda e na fase de COVID longa. Esses sintomas geralmente envolvem cognição, sono, distúrbios do olfato, manifestações psiquiátricas, dor de cabeça e outros. Esta condição é mais comumente descrita em adultos jovens e mulheres. A sintomatologia pode acompanhar casos graves ou leves da doença. A importância desta questão reside na elevada prevalência de sintomas neurológicos na fase de COVID longa, o que acarreta morbilidade significativa nesta população. Além disso, tal condição está associada a elevados custos de cuidados de saúde, com algumas estimativas em torno de 3,7 trilhões de dólares americanos. Nesta revisão, descrevemos sequencialmente o conhecimento atual sobre os sintomas neurológicos mais prevalentes na COVID longa, bem como sua fisiopatologia e possíveis biomarcadores.

How many meals and snacks do Brazilians eat a day? Findings from the 2017-2018 Brazilian National Dietary Survey / Quantas refeições e lanches os brasileiros fazem por dia? Resultados do Inquérito Nacional de Alimentação de 2017-2018 / ¿Cuántas comidas y meriendas tienen los brasileños por día? Resultados de la Encuesta Nacional de Alimentación 2017-2018

The habit of eating specific meals has been addressed in several studies, but the evaluation of meal patterns has received less attention. This study aimed to describe the meal patterns of the Brazilian population. A complex sampling design was used to select the 46,164 ≥ 10-year-old individuals examined in the Brazilian National Dietary Survey. Food consumption was assessed by two non-consecutive 24-hour recalls applied throughout a one-week period. The exploratory data analysis approach was used to determine the meal patterns, i.e., how individuals combined the main meals (breakfast, lunch, dinner) and snacks (morning, afternoon, evening/night) throughout the day. The most common meal patterns were three main meals + one snack, reported by 25.1% of the individuals, and three main meals + two snacks (24.6%). Other meal patterns identified were: three main meals + three snacks (18.5%); three main meals and no snacks (10.9%); one or two main meals + two snacks (7.4%); one or two main meals + one snack (6.9%); one or two main meals + three snacks (4.2%); and one or two main meals and no snacks (2.3%). Meal patterns varied according to gender and age group, and on typical versus atypical food consumption days. We found that eight patterns characterized the daily meal consumption in Brazil. Furthermore, around 80% of the population had three main meals every day and about 13% did not report having any snacks. The characterization of meal habits is important for tailoring and targeting health promotion actions.

Os hábitos de consumo de refeições específicas têm sido abordados em diversos estudos, no entanto, a avaliação dos padrões refeições tem recebido menos atenção. O objetivo deste estudo foi descrever os padrões de refeições da população brasileira. Um desenho amostral complexo foi utilizado para selecionar os 46.164 indivíduos de ≥ 10 anos examinados no Inquérito Nacional de Alimentação de 2017-2018. O consumo alimentar foi avaliado por meio de dois recordatórios de 24 horas não consecutivos, aplicados durante um período de uma semana. A análise exploratória de dados foi utilizada para determinar os padrões de refeições, ou seja, como os indivíduos combinam as principais refeições (café da manhã, almoço, jantar) e lanches (manhã, tarde, noite) ao longo do dia. Os padrões de refeições mais frequentes foram três refeições principais + um lanche, conforme relatado por 25,1% dos indivíduos, e três refeições principais + dois lanches (24,6%). Outros padrões de refeições identificados foram: três refeições principais + três lanches (18,5%); três refeições principais e nenhum lanche (10,9%); uma ou duas refeições principais + dois lanches (7,4%); uma ou duas refeições principais + um lanche (6,9%); uma ou duas refeições principais + três lanches (4,2%); e uma ou duas refeições principais e nenhum lanche (2,3%). Os padrões de refeições variaram de acordo com o sexo e a faixa etária, e nos dias típicos em comparação com os atípicos de consumo alimentar. Verificou-se que oito padrões caracterizaram o consumo diário de refeições no Brasil. Além disso, cerca de 80% da população realizava três refeições principais diárias e cerca de 13% reportaram não lanchar. A caracterização dos padrões de refeições é importante para adequar e direcionar ações de promoção da saúde.

Los hábitos alimenticios específicos se han abordado en varios estudios, sin embargo, poco se sabe sobre la evaluación de los patrones de alimentación. El objetivo de este estudio fue describir el patrón de alimentación de la población brasileña. Se utilizó un diseño de muestra complejo para seleccionar a 46.164 individuos de ≥ 10 años quienes participaron en la Encuesta Nacional de Alimentación 2017-2018. El consumo alimentario se evaluó mediante dos registros de 24 horas no consecutivos, aplicados durante una semana. Para determinar el patrón de alimentación, se aplicó el análisis exploratorio, es decir, cómo las personas combinan las comidas principales (desayuno, almuerzo, cena) y las meriendas (mañana, tarde, noche) a lo largo del día. Los patrones de alimentación más frecuentes fueron tres comidas principales + una merienda según informan el 25,1% de los individuos, y tres comidas principales + dos meriendas (24,6%). Otros patrones identificados destacaron tres comidas principales + tres meriendas (18,5%); tres comidas principales sin merienda (10,9%); una o dos comidas principales + dos meriendas (7,4%); una o dos comidas principales + una merienda (6,9%); una o dos comidas principales + tres meriendas (4,2%); y una o dos comidas principales sin merienda (2,3%). Los patrones de alimentación tuvieron una variación según el sexo y el grupo de edad, y en días típicos en comparación con los atípicos de consumo de alimentos. Se encontró que ocho patrones caracterizan el consumo diario de comidas en Brasil. Por lo tanto, aproximadamente el 80% de la población tienen tres comidas principales al día y aproximadamente el 13% informan que no tienen merienda. Es importante caracterizar los patrones de alimentación para adaptar y orientar las acciones de promoción de la salud.

Long COVID: neurological manifestations - an updated narrative review / COVID longa: manifestações neurológicas - uma revisão narrativa atualizada

ABSTRACT. Infection with the SARS-CoV-2 virus can lead to neurological symptoms in the acute phase and in the Long COVID phase. These symptoms usually involve cognition, sleep, smell disorders, psychiatric manifestations, headache and others. This condition is more commonly described in young adults and women. This symptomatology can follow severe or mild cases of the disease. The importance of this issue resides in the high prevalence of neurological symptoms in the Long COVID phase, which entails significant morbidity in this population. In addition, such a condition is associated with high health care costs, with some estimates hovering around 3.7 trillion US dollars. In this review, we will sequentially describe the current knowledge about the most prevalent neurological symptoms in Long COVID, as well as their pathophysiology and possible biomarkers.

RESUMO. A infecção pelo vírus SARS-CoV-2 pode levar a sintomas neurológicos na fase aguda e na fase de COVID longa. Esses sintomas geralmente envolvem cognição, sono, distúrbios do olfato, manifestações psiquiátricas, dor de cabeça e outros. Esta condição é mais comumente descrita em adultos jovens e mulheres. A sintomatologia pode acompanhar casos graves ou leves da doença. A importância desta questão reside na elevada prevalência de sintomas neurológicos na fase de COVID longa, o que acarreta morbilidade significativa nesta população. Além disso, tal condição está associada a elevados custos de cuidados de saúde, com algumas estimativas em torno de 3,7 trilhões de dólares americanos. Nesta revisão, descrevemos sequencialmente o conhecimento atual sobre os sintomas neurológicos mais prevalentes na COVID longa, bem como sua fisiopatologia e possíveis biomarcadores.

Psychosocial aspects related to fruit and vegetable consumption in adolescents / Aspectos psicossociais relacionados ao consumo de frutas e hortaliças em adolescentes

Abstract This study aimed at assessing the association between psychosocial aspects and fruit and vegetable consumption in adolescents. Cross-sectional study developed with 327 adolescents from a public school in Brazil. A questionnaire developed for adolescents was applied to assess the influence of self-efficacy, family, peers, and body image on the consumption of fruits and vegetables. Specific questions assessed the intake frequency of fruits and vegetables, and the mean consumption was estimated using two 24-hour recalls. Scores related to psychosocial aspects were described as mean and median and the weight status was classified based on the body mass index. The average daily consumption of fruits and vegetables was 36.2 g and 45.4 g, respectively. Self-efficacy and the influence of peers were associated with an increase in the amount and frequency of fruits and vegetables consumption. Adolescents with higher scores of self-efficacy had greater average consumption of fruits and vegetables when compared to those with lower scores. The same was observed for the influence of peers. Self-efficacy and the influence of peers were the factors that most influenced the consumption of fruits and vegetables in adolescents.

Resumo O objetivo do estudo foi avaliar a associação entre aspectos psicossociais e o consumo de frutas e hortaliças em adolescentes. Estudo transversal desenvolvido com 327 adolescentes de uma escola pública do Brasil. Um questionário desenvolvido para adolescentes foi aplicado para avaliar a influência da autoeficácia, família, pares e imagem corporal sobre o consumo de frutas e hortaliças. Questões específicas avaliaram a frequência de ingestão de frutas e hortaliças e o consumo foi estimado por meio de dois recordatórios de 24 horas. Os escores relacionados aos aspectos psicossociais foram descritos por média e mediana e a condição de peso foi classificada com base no índice de massa corporal. O consumo diário médio de frutas e hortaliças foi de 36,2 g e 45,4 g, respectivamente. A autoeficácia e a influência dos pares associaram-se ao aumento da quantidade e da frequência de consumo de frutas e hortaliças. Os adolescentes com maiores valores no escore para autoeficácia apresentaram maior média de consumo de frutas e hortaliças quando comparados àqueles com menores escores. O mesmo foi observado para a influência dos pares. A autoeficácia e a influência dos pares foram os fatores que mais influenciaram o consumo de frutas e hortaliças em adolescentes.

Evolution of food intake estimates in Brazil: the 2008-2009 and 2017-2018 National Dietary Surveys / Evolução das estimativas da quantidade dos alimentos consumidos no Brasil: Inquérito Nacional de Alimentação 2008-2009 e 2017-2018

ABSTRACT Objective To present changes in the estimated amount of food intake in Brazil between the 2008-2009 and 2017-2018 National Dietary Surveys. Methods Food intake data from the 2008-2009 and 2017-2018 surveys were used to highlight the differences in the frequencies of foods mentioned, the number of the measurement units mentioned, and the frequency of measurements that were incompatible with the reported food and were exchanged by the most mentioned measurement (standard measurement), as well as to describe the updates performed in the database between edits. Results The elaboration of the 2017-2018 referenced measurement table was based on the 2008-2009 table, which was revised and updated. In the 2008-2009 survey, 9980 household measurements were mentioned for 1970 types of food and preparations, while in 2017-2018 there were 11050 and 2534, respectively. While in 2008-2009, 2.8% of citations were replaced by the standard measurement, in 2017-2018, only 0.7% of food items needed to be replaced. Conclusion The procedures used to estimate the amount of food intake between the surveys allowed updating the table of household measurements and minimizing errors in the estimate of this amount, with a reduction in measurement units that were inconsistent or incompatible with the aforementioned foods.

RESUMO Objetivo Apresentar a evolução na estimativa da quantidade dos alimentos consumidos no Brasil entre os Inquéritos Nacionais de Alimentação de 2008-2009 e de 2017-2018. Métodos Foram utilizados dados de consumo alimentar de 2008-2009 e de 2017-2018 para evidenciar as diferenças nas frequências de citações, nos números de unidades de medidas citadas e na frequência de medidas incompatíveis com o alimento que foram substituídas pela medida citada com maior frequência (medida padrão), bem como descrever as atualizações realizadas no banco de dados entre as edições. Resultados A construção da tabela de medidas referidas de 2017-2018 foi baseada na tabela de 2008-2009, a qual foi revisada e atualizada. No inquérito de 2008-2009 foram citadas 9980 medidas caseiras para 1970 alimentos e preparações, enquanto em 2017-2018 foram 11050 para 2534, respectivamente. Enquanto em 2008-2009 2,8% das citaçoes foram substituídas pela medida padrao, em 2017-2018, somente 0,7% dos alimentos precisaram ser substituídos. Conclusão Os procedimentos utilizados na estimativa de quantidade de alimentos consumidos entre os inquéritos permitiram atualizar a tabela de medidas caseiras e minimizar erros na estimativa dessa quantidade, com redução de unidades de medidas incoerentes ou incompatíveis com os alimentos citados.

Acquisition of food for away-from-home consumption in Brazil between 2002 and 2018 / Aquisição de alimentos para consumo fora do domicílio no Brasil entre 2002 e 2018

Abstract The aim of the study was to assess the evolution of food acquisition for away from home consumption in Brazil from 2002 to 2018. The trend of food purchases for out-of-home consumption in Brazil was evaluated by comparing food purchase data from the Household Budget Surveys (HBS) of 2002-2003, 2008-2009, and 2017-2018. The frequency of food acquisition was estimated according to sociodemographic variables and the mean cost. In 2002-2003, the frequency of purchase of food for out-of-home consumption was 35.2% (95%CI: 34.4-35.9), increasing to 41.2% (95%CI: 40.4-42.0) in 2008-2009, followed by a decline in 2017-2018 (32.3%; 95%CI: 31.7-32.9). A declining trend was observed in the frequency of purchases of alcoholic beverages and soft drinks and fast foods maintained the frequency between the last two surveys. Spending on this type of food increased between 2002-2003 and 2008-2009, while the mean value of this type of expenditure was maintained between 2008-2009 and 2017-2018. Brazilians increased food purchases for out-of-home consumption between 2002-2003 and 2008-2009, declining in 2017-2018. A consistent fall in the purchase of alcoholic beverages and soft drinks was observed over time, while the group of meals grew significantly.

Resumo O objetivo desse estudo foi avaliar a evolução da aquisição de alimentos para consumo fora de casa no Brasil de 2002 a 2018. A tendência de compra de alimentos para consumo fora de casa no Brasil foi avaliada pela comparação de dados de compra de alimentos das Pesquisas de Orçamentos Familiares (POF) de 2002-2003, 2008-2009 e 2017-2018. A frequência de aquisição de alimentos foi estimada de acordo com variáveis sociodemográficas e o custo médio. Em 2002-2003, a frequência de compra de alimentos para consumo fora do domicílio foi de 35,2% (IC95%: 34,4-35,9), aumentando para 41,2% (IC95%: 40,4-42,0) em 2008-2009, seguido por um declínio em 2017-2018 (32,3%; IC95%: 31,7-32,9). Foi observada tendência de queda na frequência de compras de bebidas alcoólicas e refrigerantes, enquanto fast food manteve a frequência entre as duas últimas pesquisas. Os gastos com esse tipo de alimentação aumentaram entre 2002-2003 e 2008-2009, enquanto o valor médio desse tipo de gasto se manteve entre 2008-2009 e 2017-2018. Os brasileiros aumentaram as compras de alimentos para consumo fora de casa entre 2002-2003 e 2008-2009, diminuindo em 2017-2018. Uma queda consistente na compra de bebidas alcoólicas e refrigerantes foi observada ao longo do tempo, enquanto o grupo das refeições apresentou aumento significativo.

Evolution of out-of-home food consumption in Brazil in 2008-2009 and 2017-2018 / Evolução do consumo de alimentos fora do domicílio no Brasil de 2008-2009 a 2017-2018

ABSTRACT OBJECTIVE To describe the evolution of out-of-home food consumption in Brazil in 2008-2018. METHODS We used the 2008-2009 and 2017-2018 data from the Inquéritos Nacionais de Alimentação (INA - National Food Surveys), conducted amid 34,003 and 46,164 individuals, to estimate the frequency of out-of-home food consumption and the contribution of this consumption to specific foods. Food consumption was analyzed using food records in the 2008-2009 INA and 24-hour recalls in 2017-2018. Estimates were generated for Brazil in general, for urban and rural areas, for age groups (adolescent, adult, elderly), and for income bracket. RESULTS The frequency of out-of-home consumption decreased by 8.8% between the two surveys, with no change in the rural area, in the Northeast and South regions, and for the lowest income brackets. We observed a slight increase among the elderly and in the Midwest region. The contribution of out-of-home food consumption to daily energy intake also decreased (16.3% vs. 12.7%), excepting the rural area, where there was a reduction in the difference in relation to the urban area between the two surveys. For most items evaluated, the out-of-home food consumption decreased. The most consumed out-of-home food were alcoholic beverages, fried and baked snacks, soft drinks, pizza, sweets, and sandwiches in both surveys. CONCLUSION In 10 years, the prevalence of food consumption and the percentage of contribution of out-of-home food decreased in Brazil, but ultra-processed foods still figure as the most consumed food group outside the home.

RESUMO OBJETIVO Descrever a evolução do consumo de alimentos fora do domicílio no Brasil no período 2008-2018. MÉTODOS Foram usados dados dos Inquéritos Nacionais de Alimentação (INA) de 2008-2009 e 2017-2018, realizados com 34.003 e 46.164 indivíduos, respectivamente, para estimar a frequência de consumo de alimentos fora de casa e a contribuição desse consumo para alimentos específicos. O consumo de alimentos foi investigado por meio de registros alimentares no INA 2008-2009 e de recordatórios 24 horas em 2017-2018. As estimativas foram geradas para o Brasil como um todo, para áreas urbana e rural, para faixas de idade (adolescente, adulto, idoso) e para faixas de renda. RESULTADOS A frequência de consumo fora de casa diminuiu 8,8% entre os dois inquéritos, sem alteração na área rural, nas regiões Nordeste e Sul e nas menores faixas de renda. Houve leve aumento entre idosos e na região Centro-Oeste. De modo geral, a contribuição do consumo alimentar fora de casa para a ingestão diária de energia também diminuiu (16,3% vs. 12,7%), com exceção da área rural, onde houve redução da diferença em relação à área urbana entre os dois inquéritos. O consumo de alimentos fora de casa foi reduzido para a maioria dos itens avaliados. Os alimentos mais consumidos fora de casa em ambos os inquéritos foram as bebidas alcoólicas, salgadinhos fritos e assados, refrigerantes, pizzas, doces e sanduíches. CONCLUSÃO Em 10 anos, a prevalência de consumo de alimentos e o percentual de contribuição dos alimentos consumidos fora de casa diminuíram no Brasil, mas os alimentos ultraprocessados ainda figuram como o grupo de alimento mais consumido fora de casa.

Relação entre Transtornos Mentais Comuns e a ingestão dietética de universitários da área da saúde / Association between common mental disorders and dietary intake among university students doing health-related courses

Resumo O objetivo foi identificar a relação entre Transtorno Mental Comum (TMC) e a ingestão dietética de universitários da área saúde. Foram entrevistados 432 estudantes, regularmente matriculados em um dos cursos da área da saúde em uma universidade pública, no município de Fortaleza, Ceará, entre abril e dezembro de 2018. Utilizou-se o Self Reporting Questionnaire-20 (SRQ-20) para rastreio de TMC. O consumo alimentar foi avaliado por meio do Recordatório de 24 horas e, para a avaliação da atividade física, utilizou-se o International Physical Activity Questionnaire (IPAQ), versão curta. Modelos de regressão linear foram utilizados para avaliar a relação entre ingestão de energia e nutrientes, e rastreamento positivo para TMC. Os modelos foram ajustados por idade, sexo e atividade física. A prevalência de TMC foi de 44,5%, sendo maior nas mulheres. Os indivíduos com rastreamento positivo para TMC apresentaram menor frequência de atividade física e maior média de ingestão de açúcar de adição, gordura saturada e menor média de ingestão de sódio e fibras, independente da idade, sexo e atividade física. O presente trabalho demonstrou altas prevalências de TMC entre os universitários, estando associada com maior ingestão de açúcar de adição e gordura saturada e menor ingestão de fibras.

Abstract The objective of this study was to determine the association between common mental disorders (CMD) and dietary intake among Brazilian undergraduate students doing health-related courses. We interviewed 432 students enrolled at a public university in Fortaleza, the capital of the State of Ceará, between April and December 2018. The students were screened for CMD using the Self-Reporting Questionnaire (SRQ-20). Food consumption and physical activity were assessed using a 24-hour dietary recall and the International Physical Activity Questionnaire short form (IPAQ-SF), respectively. We used linear regression to measure the association between energy and nutrient intake and positive screening for CMD. The models were adjusted for age, gender and physical activity. Overall prevalence of CMD was 44.5% and the rate was higher in women than in men. Individuals who screened positive for CMD practiced less physical activity and reported higher mean intake of added sugar and saturated fat and lower mean fiber intake, regardless of age, gender, and level of physical activity. Our findings show that the prevalence of CMD among undergraduate students doing health-related courses was high and that the presence of potential CMD was associated with higher intake of added sugar and saturated fats and lower fiber intake.

Evolução da ingestão de energia e nutrientes de adolescentes de escolas públicas de Niterói, Rio de Janeiro, Brasil, 2003-2008 / Trends in energy and nutrient intake by adolescents in public schools in Niterói, Rio de Janeiro State, Brazil, 2003-2008 / Evolución de la ingestión de energía y nutrientes en adolescentes de escuelas públicas de Niteroi, Río de Janeiro, Brasil, 2003-2008

Resumo: Analisaram-se as variações na ingestão de energia e nutrientes de adolescentes, estudantes de escolas públicas, examinados em dois estudos de base escolar, com desenho seccional, realizados em 2003 e 2008. O consumo alimentar foi avaliado com a aplicação de três registros alimentares. A condição de peso foi classificada segundo os critérios da Organização Mundial da Saúde. Em 2003, foram investigados 433 adolescentes, e, em 2008, 510 adolescentes. A prevalência de excesso de peso foi de 17%, em 2003, e de 22%, em 2008 (p > 0,05). Observou-se redução na ingestão de gorduras saturadas e de vitamina A entre os meninos. Entre as meninas, houve incremento na ingestão de energia, carboidratos e cálcio e redução para proteína e ferro. Ambos os grupos reduziram a ingestão de vitamina E e de lipídios e aumentaram a de sódio. Entre os adolescentes sem excesso de peso, observou-se incremento da ingestão de cálcio, e, para aqueles com peso excessivo, houve redução de gordura saturada e de vitamina A. No decorrer de cinco anos, a dieta dos adolescentes sofreu prejuízo da qualidade nutricional.

Abstract: The study analyzed variations in energy and nutrient intake by adolescents enrolled in public schools, examined in two school-based cross-sectional surveys, in 2003 and 2008. Food consumption was assessed with three food records. Weight was classified according to World Health Organization criteria. A total of 433 adolescents were studied in 2003 and 510 in 2008. Prevalence of excess weight was 17% in 2003 and 22% in 2008 (p > 0.05). There was a reduction in the intake of saturated fats and vitamin A in boys. Girls showed an increase in the intake of energy, carbohydrates, and calcium and a reduction in protein and iron. Both boys and girls reduced their intake of vitamin E and lipids and increased their sodium. Adolescents with excess weight showed an increase in calcium intake and a decrease in saturated fat and vitamin A. The nutritional quality of the adolescents' diet declined over the course of the five years.

Resumen: Se analizaron las variaciones en la ingestión de energía y nutrientes en estudiantes adolescentes de escuelas públicas, examinados en dos estudios de base escolar, con diseño seccional, realizados en 2003 y 2008. El consumo alimenticio fue evaluado con la aplicación de tres registros alimentarios. La condición de peso fue clasificada según los criterios de la Organización Mundial de la Salud. En 2003, se investigaron a 433 adolescentes y en 2008, a 510 adolescentes. La prevalencia de exceso de peso fue de un 17% en 2003 y de un 22% en 2008 (p > 0,05). Se observó una reducción en la ingestión de grasas saturadas y vitamina A entre los niños. Entre las niñas, hubo un incremento en la ingestión de energía, carbohidratos y calcio y reducción para proteína e hierro. Ambos grupos redujeron la ingestión de vitamina E y lípidos y aumentaron la de sodio. Entre los adolescentes sin exceso de peso se observó un incremento de la ingestión de calcio, y para aquellos con peso excesivo hubo reducción de grasa saturada y vitamina A. En el transcurso de cinco años la dieta de los adolescentes sufrió un perjuicio de su calidad nutricional.

Modificações no consumo de bebidas de adolescentes de escolas públicas na primeira década do século XXI / Changes in beverage consumption among adolescents from public schools in the first decade of the century XXI

RESUMO: Objetivo: Avaliar mudanças no consumo de bebidas em adolescentes entre 2003 e 2008. Métodos: Foram realizados dois estudos transversais de base escolar com estudantes de 12 a 19 anos de idade de escolas públicas de Niterói, Rio de Janeiro. Dados de três registros alimentares foram utilizados para estimar o consumo médio diário, de dias de semana e de final de semana (volume e a contribuição para a ingestão diária de energia) de leite, bebidas à base de leite, bebidas com adição de açúcar, suco de frutas frescas, bebidas cafeinadas e bebidas alcoólicas. Foram utilizados Modelos Lineares Generalizados (GLM) para estimar médias ajustadas por idade e as diferenças nos estimadores segundo os dias de semana. Resultados: Foram investigados 433 adolescentes em 2003 e 510 foram investigados em 2008. A prevalência de excesso de peso foi de 17% em 2003 e de 22% em 2008 (p > 0,05). O leite foi a bebida mais consumida, sendo relatada por 89% dos adolescentes, seguido dos refrigerantes (75%). De maneira geral, no período de cinco anos, verificou-se aumento da prevalência de consumo de bebidas alcoólicas, bebidas à base de xarope de guaraná e sucos processados, especialmente nos dias de semana. O refrigerante foi a bebida que mais contribuiu para o consumo energético total, correspondendo, em média, a 4% da ingestão energética diária. Conclusão: As principais mudanças no perfil de consumo de bebidas entre os adolescentes de Niterói na primeira década do século XXI foram a tendência para redução do consumo de leite e o aumento no consumo das bebidas processadas e das bebidas alcoólicas.

ABSTRACT: Objective: To evaluate the changes in beverage consumption among adolescents between 2003 and 2008. Methods: Two school-based cross-sectional studies were carried out with public school students (12 to 19 years-old) from Niterói, Rio de Janeiro, Brazil. Data from three food records were used to estimate daily, weekdays and weekend average consumption (volume and percent contribution for total daily energy intake) of milk and milk-based beverages, sugar sweetened beverages, fresh squeezed fruit juices, caffeinated and alcoholic beverages. Beverage consumption age-adjusted means for weekdays and weekends were compared using linear regression (Generalized Linear Models - GLM). Results: A total of 433 adolescents were examined in 2003, and 510 in 2008. The prevalence of overweight was 17% in 2003 and 22% in 2008 (p > 0.05). Milk was the most consumed beverage, being reported by 89% of adolescents, followed by sodas (75%). In general, in the five-year period, there was an increase in the prevalence of consumption of alcoholic drinks, guarana syrup refreshment, and processed fruit drinks, especially on weekdays. The soft drink was the largest contributor to the total energy consumption, corresponding on average to 4% of daily energy intake. Conclusion: The main changes in the beverage consumption among adolescents from Niterói, in the first decade of the XXI century, were the tendency to reduce the consumption of milk and the increase in the consumption of processed and alcoholic beverages.

Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii / Eficácia de Trichoderma asperellum, T. harzianum, T. longibrachiatum E T. reesei contra Sclerotium rolfsii

This work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and the following ones were selected in vitro for the in vivo tests: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642, 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) and EST 5. The most promising isolates were identified by Sequencing of the internal transcribed spacer regions ITS1, ITS2, and the 5.8s rRNA genomic region, using the ITS5 and ITS4 primers and compared with sequences in the National Center for Biotechnology Information (NCBI) database. These selected isolates 1649 (T. harzianum), 1525 (T. longibrachiatum) and 1637 (T. reesei) were tested for evaluation of sclerotial germination inhibition under laboratory conditions, and to evaluate the effects of these on disease of bean plants under greenhouse conditions. The Trichoderma isolates 1649, 1525 and 1637 were more efficient in reducing sclerotial germination. In addition to 1649, 1525 and 1637, the isolates 5, 12, 102 and 1525 (T. longibrachiatum) significantly reduced de amount of diseased bean plants under greenhouse conditions.

O estudo teve como objetivo selecionar e testar isolados de Trichoderma spp. para o controle da murcha-por-esclerócio (Sclerotium rolfsii) em feijoeiro (Phaseolus vulgaris) em casa-de-vegetação. Nos ensaios realizados foram utilizados dois isolados de S. rolfsii (UB 193 e UB 228). Dos 65 isolados Trichoderma testados in vitro selecionaram-se os seguintes: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642 (T. longibrachiatum), 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) e EST 5. Os isolados mais promissores foram identificados por sequenciamento das regiões genômicas ITS1, ITS2 e 5.8s rRNA, usando iniciadores ITS5 e ITS4, e então, tais sequencias foram comparadas com as sequências do banco de dados do "National Center for Biotechnology Information" (NCBI). Esses isolados foram avaliados quanto ao efeito sobre a germinação de esclerócios do patógeno em laboratório e sobre a doença em casa de vegetação. Os isolados 1649 (T. harzianum), 1525 (T. longibrachiatum) e 1637 (T. reesei) foram os mais eficientes na inibição da germinação de esclerócios de S. rolfsii em laboratório. Além dos isolado 1649, 1525 e 1637, os isolados 5, 12, 102 e 1525 (T. longibrachiatum) foram eficientes na redução da doença em plantas de feijoeiro em casa de vegetação.