Comparison between eight- and sixteen-channel TEM transceive arrays for body imaging at 7 T.

Eight- and sixteen-channel transceive stripline/TEM body arrays were compared at 7 T (297 MHz) both in simulation and experiment. Despite previous demonstrations of similar arrays for use in body applications, a quantitative comparison of the two configurations has not been undertaken to date. Results were obtained on a male pelvis for assessing transmit, signal to noise ratio, and parallel imaging performance and to evaluate local power deposition versus transmit B(1) (B(1) (+) ). All measurements and simulations were conducted after performing local B(1) (+) phase shimming in the region of the prostate. Despite the additional challenges of decoupling immediately adjacent coils, the sixteen-channel array demonstrated improved or nearly equivalent performance to the eight-channel array based on the evaluation criteria. Experimentally, transmit performance and signal to noise ratio were 22% higher for the sixteen-channel array while significantly increased reduction factors were achievable in the left-right direction for parallel imaging. Finite difference time domain simulations demonstrated similar results with respect to transmit and parallel imaging performance, however, a higher transmit efficiency advantage of 33% was predicted. Simulations at both 3 and 7 T verified the expected parallel imaging improvements with increasing field strength and showed that, for a specific B(1) (+) shimming strategy used, the sixteen-channel array exhibited lower local and global specific absorption rate for a given B(1) (+) .

Initial results of cardiac imaging at 7 Tesla.

This work reports preliminary results from the first human cardiac imaging at 7 Tesla (T). Images were acquired using an eight-channel transmission line (TEM) array together with local B(1) shimming. The TEM array consisted of anterior and posterior plates closely positioned to the subjects' thorax. The currents in the independent elements of these arrays were phased to promote constructive interference of the complex, short wavelength radio frequency field over the entire heart. Anatomic and functional images were acquired within a single breath hold to reduce respiratory motion artifacts while a vector cardiogram (VCG) was used to mitigate cardiac motion artifacts and gating. SAR exposure was modeled, monitored, and was limited to FDA guidelines for the human torso in subject studies. Preliminary results including short-axis and four-chamber VCG-retrogated FLASH cines, as well as, short-axis TSE images demonstrate the feasibility of safe and accurate human cardiac imaging at 7T.

Binocular rivalry and visual awareness in human extrastriate cortex.

We used functional magnetic resonance imaging (fMRI) to monitor stimulus-selective responses of the human fusiform face area (FFA) and parahippocampal place area (PPA) during binocular rivalry in which a face and a house stimulus were presented to different eyes. Though retinal stimulation remained constant, subjects perceived changes from house to face that were accompanied by increasing FFA and decreasing PPA activity; perceived changes from face to house led to the opposite pattern of responses. These responses during rivalry were equal in magnitude to those evoked by nonrivalrous stimulus alternation, suggesting that activity in the FFA and PPA reflects the perceived rather than the retinal stimulus, and that neural competition during binocular rivalry has been resolved by these stages of visual processing.

The retinotopy of visual spatial attention.

We used high-field (3T) functional magnetic resonance imaging (fMRI) to label cortical activity due to visual spatial attention, relative to flattened cortical maps of the retinotopy and visual areas from the same human subjects. In the main task, the visual stimulus remained constant, but covert visual spatial attention was varied in both location and load. In each of the extrastriate retinotopic areas, we found MR increases at the representations of the attended target. Similar but smaller increases were found in V1. Decreased MR levels were found in the same cortical locations when attention was directed at retinotopically different locations. In and surrounding area MT+, MR increases were lateralized but not otherwise retinotopic. At the representation of eccentricities central to that of the attended targets, prominent MR decreases occurred during spatial attention.

Is the intracellular pH different from normal in the epileptic focus of patients with temporal lobe epilepsy? A 31P NMR study.

We performed in vivo 31P NMR spectroscopic studies of human brain on a 4.1 T whole-body NMR system. Based on a control group of 20 healthy volunteers, the normal pHi was 7.05 (SD, 0.06; SEM, 0.01) in the left temporal lobe and 7.04 (SD, 0.04; SEM, 0.01) in the right temporal lobe. We also studied a patient group consisting of 13 individuals with unilateral temporal lobe epilepsy. The mean pHi was 7.02 (SD, 0.04; SEM, 0.01) in the ipsilateral lobe and 7.02 (SD, 0.05; SEM, 0.01) in the contralateral lobe. These results clearly show that no statistically significant difference in pHi is observed between the two lobes, either in normal controls or in patients. Also, no significant pHi difference exists between the control group and the patient group. Lateralization in each of the 13 patients with unilateral epilepsy, based on their individual pHi difference between the ipsilateral lobe and contralateral lobe (delta pHi), showed that three patients were nondiagnostic cases because their delta pHis were not significantly different from zero (< or = 0.02), five patients showed small delta pHis consistent with their clinical lateralization, whereas the remaining five patients showed delta pHi-based lateralization opposite to the clinical findings. These results seem to indicate an essentially random distribution around delta pHi = 0 within a very small experimental error of +/-0.02 pH units. pHi obtained from eight different areas in each of the 13 unilateral patients also did not show any significantly nonzero delta pHi values. These results led to the conclusion that even at the excellent spectral resolution and reproducibility of the 4.1 T machine (typical SD of 0.05 pH units), no significant pHi effect, induced by temporal lobe epilepsy, could be detected. Therefore, in this study, delta pHi does not appear to be a clinically useful tool for the lateralization of epileptic foci in patients with temporal lobe epilepsy.

Functional imaging of brain activity in conscious monkeys responding to sexually arousing cues.

Olfactory cues can elicit intense emotional responses. This study used fMRI in male common marmoset monkeys to identify brain areas associated with sexual arousal in response to odors of ovulating female monkeys. Under light anesthesia, monkeys were secured in a specially designed restrainer and positioned in a 9.4 T magnetic resonance spectrometer. When fully conscious, they were presented with the scents of both ovariectomized and ovulating monkeys. The sexually arousing odors of the ovulating monkeys enhanced signal intensity in the preoptic area and anterior hypothalamus compared to the odors of ovariectomized monkeys. These data corroborate previous findings in monkeys based on invasive electrical lesion and stimulation techniques and demonstrate the feasibility of using non-invasive functional imaging on fully conscious common marmosets to study cue-elicited emotional responses.

Evaluations of magnetic resonance imaging parameters with simple phantoms.

Imaging parameters associated with a clinical magnetic resonance imaging (MRI) system, evaluated with the use of simple and inexpensive phantoms, are described in evaluation of a clinical MRI system. Images, obtained with a Diasonics 0.35-T MRI system using an elliptical whole-body radio frequency coil, are presented which demonstrate geometric distortion, inaccuracy of image dimensions, and artifacts. Measurements of reproducibility and uniformity of both signal intensity and spin-lattice relaxation times are presented for uniform phantoms. Replicate measurements are analyzed by two-way analyses of variance to determine the significance of variations as a function of position and slice. Apparent T1's are not significantly different among the slices for the images analyzed, but there is a significant effect due to position in the field. Direct measurement of the radio frequency field for the center slice follows a pattern which is similar. Spin-lattice relaxation times are compared among two-point calculations and nonlinear four-point calculations, and the effects of pooling data are found to increase the precision of T1 measurements.

High resolution neuroimaging at 4.1T.

In this article we report on acquisition of high resolution 512 x 512 images at 4.1T using an inversion recovery gradient-echo sequence and a volume head coil developed for high field applications. The Ti values for cerebral white and grey matter were measured to be 834 and 1282 ms, respectively. The partial saturation inversion recovery sequence (Tir 800 ms and TR 2500 ms) provided excellent contrast-to-noise for white to grey matter. Consequently, the images consistently visualized the thalamic nuclear groups, hippocampal fine structure, as well as small draining vessels of the white matter.

Application of high field spectroscopic imaging in the evaluation of temporal lobe epilepsy.

Previous spectroscopic imaging studies of temporal lobe epilepsy have used comparisons of metabolite content or ratios to lateralize the seizure focus. Although highly successful, these studies have shown significant variations within each of the groups of healthy subjects and patients. This variation may arise from the natural differences seen in metabolite concentration in gray and white matter, the complex anatomy seen about the hippocampus, and the large voxels typically employed at 1.5 T. Using a 4.1 T whole body system, we have acquired spectroscopic images with 0.5 cc nominal voxels (1 cc after filtering) to evaluate the regional variation in metabolite content of the hippocampus, temporal gray and white matter, midbrain, and cerebellar vermis. Using a threshold value of 0.90 for CR/NAA, a value 90% of all normal hippocampal voxels lay below, we have correctly identified the presence of epileptogenic tissue in patients with unilateral as well as bilateral seizures. By using comparisons to healthy values of the CR/NAA ratio, this method enables the visualization of bilateral disease and provides information on the extent of gray matter involvement.

Vascular anatomy and surgical technique for bilateral adrenalectomy in the equid.

The vascular anatomy of the adrenal glands and a surgical technique for bilateral adrenalectomy in the equid are described. Bilateral adrenalectomy was performed in six ponies and one horse via bilateral transcostal retroperitoneal approaches through the 18th rib during a single anesthetic period. Complications included hemorrhage from the right side only and pneumothorax which usually occurred on the right side. One pony died as a result of the surgical technique. Maintenance of adrenal function was accomplished with desoxycorticosterone pivilate and prednisolone.

Thermography: a technique for subclinical diagnosis of osteoarthritis.

Thermographic and radiographic evaluations of the tarsus (hock) were done on 20 Standardbred racehorses before and after exercise at three consecutive 6-week intervals. All horses were from the same stable and given the same care and training under identical schedules and conditions. Normal thermographic patterns were established before and after exercise. These patterns corresponded to the underlying tarsal vasculature. Postexercise thermal patterns indicated a warming trend, and the increases were uniform. Abnormal thermal patterns were more localized and did not conform to the normal underlying vascular distribution. Five of the 20 horses trained successfully and competed professionally, with 1 of the 5 showing abnormal thermographic changes. Four horses were too slow to race, and all of these had abnormal thermographic changes of their tarsi. The medial aspect of the right tarsus was more commonly involved than the lateral in these horses. Only one horse was clinically lame and exhibited thermal increases, as well as radiographic changes in the right tarsus. The remaining horses were removed from training due to miscellaneous causes. It was believed that the four horses that failed to make minimum track times (for racing) suffered discomfort in their tarsi sufficient to impair performance. This problem was attributed to early subclinical inflammatory changes within the joints. Though difficult to recognize radiographically, these early changes were conspicuous as abnormal thermographic patterns typical of inflammation.

Electroencephalographic patterns of clinically normal, sedated, and tranquilized newborn foals and adult horses.

To establish a clinically practical procedure for recording the equine EEG, 25 healthy adult horses and 6 newborn foals were used. Recordings were taken with the animals alert and tranquilized, confined in metal stocks, or physically restrained. The dominant alert waveforms of adult horses were fast activity (25 to 40 Hz) with medium-to-low voltages (5 to 40 microV-dominant 10 to 15 microV). Underlying this fast activity was slower (0.5 to 4.0 Hz) activity with medium-to-low voltages (10 to 40 microV). Twelve of the 25 adult horses had EEG frequencies in the alpha frequency range (10 to 15 Hz, 10 to 50 microV). Eight horses were given xylazine and 17 were given acetylpromazine. Those given xylazine had generalized slowing with several distinct frequency patterns (25 to 40 Hz, 5 to 30 microV; 10 to 15 Hz, 10 to 80 microV; and 0.5 to 4.0 Hz, 10 to 90 microV). Horses given acetylpromazine had fast activity (25 to 40 Hz) with medium-to-low voltages (5 to 40 microV). Underlying this activity were slower waveforms (1 to 4 Hz) with medium-to-low voltages (5 to 10 microV). Occasional well-formed spindle activity was observed (10 to 14 Hz, 10 to 50 microV). Acetylpromazine had little effect on the EEG recording, whereas xylazine exerted a substantial effect. All leads were synchronous with lower voltages in the left frontal, right frontal, and transfrontal leads. The alert pattern of a newborn foal was characterized by low frequency (2 to 6 Hz) with medium-to-high voltages (20 to 90 microV).(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of the fungal endophyte Acremonium coenophialum in fescue on pregnant mares and foal viability.

Effects of the endophyte Acremonium coenophialum in tall fescue on pregnant mares and foal viability were evaluated. Twenty-two mature pregnant mares were randomly chosen to graze either Kentucky-31 tall fescue that was free from A coenophialum (endophyte-free, EF) or tall fescue infected with A coenophialum (endophyte-present, EP) after the first 90 days of pregnancy through parturition. Concentrations of pyrrolizidine and ergopeptine alkaloids were significantly greater in EP grass, compared with EF pasture. Ten of 11 mares grazing EP pasture had obvious dystocia. Mean duration of gestation was significantly greater for the EP group, compared with the EF group. Foal survivability was severely reduced among mares grazing EP fescue with only 1 foal surviving the natal period. Udder development and lactation were low in mares grazing EP grass. The absence of clinical problems in mares grazing EF grass implicated the endophyte as the causative agent of reproductive problems and perinatal foal mortality in pregnant mares grazing endophyte-infected fescue grass. Caution should be exercised in allowing pregnant mares to graze pastures infected with the endophyte A coenophialum.

Effects of denervation of the digit of the horse.

A study was conducted in an effort to explain why digital necrosis sometimes follows neurectomy in the horse. Six horses were subjected to unilateral section of the medial and lateral palmar nerves. Arteriography was done on each digit prior to surgery. Terminally, arteriography was repeated. Sections of bone, nerve, artery, skin, coronary band, and deep flexor tendon were examined histologically. Changes in arterial pattern and bone quality were noted. During the study, 2 of the horses had clinical signs of digital necrosis. The composite findings suggested trauma or infection, or both, of the denervated digit as the main cause(s) of the digital necrosis. Osteolysis was believed to be the primary contributing factor in disruption and collapse of the supporting structures of the foot.