Phylogenetic placement of the monotypic Baolia (Amaranthaceae s.l.) based on morphological and molecular evidence.

BACKGROUND: Baolia H.W.Kung & G.L.Chu is a monotypic genus only known in Diebu County, Gansu Province, China. Its systematic position is contradictory, and its morphoanatomical characters deviate from all other Chenopodiaceae. Recent study has regarded Baolia as a sister group to Corispermoideae. We therefore sequenced and compared the chloroplast genomes of this species, and resolved its phylogenetic position based on both chloroplast genomes and marker sequences. RESULTS: We sequenced 18 chloroplast genomes of 16 samples from two populations of Baolia bracteata and two Corispermum species. These genomes of Baolia ranged in size from 152,499 to 152,508 bp. Simple sequence repeats (SSRs) were primarily located in the LSC region of Baolia chloroplast genomes, and most of them consisted of single nucleotide A/T repeat sequences. Notably, there were differences in the types and numbers of SSRs between the two populations of B. bracteata. Our phylogenetic analysis, based on both complete chloroplast genomes from 33 species and a combination of three markers (ITS, rbcL, and matK) from 91 species, revealed that Baolia and Corispermoideae (Agriophyllum, Anthochlamys, and Corispermum) form a well-supported clade and sister to Acroglochin. According to our molecular dating results, a major divergence event between Acroglochin, Baolia, and Corispermeae occurred during the Middle Eocene, approximately 44.49 mya. Ancestral state reconstruction analysis showed that Baolia exhibited symplesiomorphies with those found in core Corispermoideae characteristics including pericarp and seed coat. CONCLUSIONS: Comparing the chloroplast genomes of B. bracteata with those of eleven typical Chenopodioideae and Corispermoideae species, we observed a high overall similarity and a one notable noteworthy case of inversion of approximately 3,100 bp. of DNA segments only in two Atriplex and four Chenopodium species. We suggest that Corispermoideae should be considered in a broader sense, it includes Corispermeae (core Corispermoideae: Agriophyllum, Anthochlamys, and Corispermum), as well as two new monotypic tribes, Acroglochineae (Acroglochin) and Baolieae (Baolia).

Evolutionary relationships, biogeography and morphological characters of Glinus (Molluginaceae), with special emphasis on the genus composition in Sub-Saharan Africa.

Glinus is a small genus of Molluginaceae with 8-10 species mostly distributed in the tropics of the World. Its composition and evolutionary relationships were poorly studied. A new molecular phylogeny constructed here using nuclear (ITS) and chloroplast (rbcL, trnK-matK) markers confirmed the monophyly of the genus. Based on ITS analysis, the following well-supported lineages are present within Glinus: the G. bainesii lineage is recovered as sister to the remainder of the genus followed by G. oppositifolius. Three other clades are: G. hirtus with G. orygioides; G. radiatus and G. lotoides; the latter is represented by a sample from North America, and G. zambesiacus as sister to G. setiflorus + G. lotoides + G. dictamnoides. On the plastid gene tree, G. bainesii + G. oppositifolius form a sister clade to all other Glinus species. The next clade is formed by G. hirtus and G. orygioides followed by G. radiatus plus an American sample of G. lotoides. The next branch comprises G. setiflorus as sister to G. zambesiacus + G. lotoides + G. dictamnoides. Glinus seems to have originated from Africa around the Late Eocene or Early Miocene, with further radiations to Australia and the Americas during the Late Miocene or Late Pliocene. Compared with the previous limited character set used for the diagnostics, we have found ten new morphological and carpological traits distinguishing Glinus members. In both trees based on nuclear and plastid datasets, the major phylogenetic clades cannot be characterized by the peculiar morphological characters. Many shared character states leading to their contrasting pattern in the multivariate analysis model are interpreted as a high homoplasy in the phylogenetically distant species. We paid special attention to the composition of the genus in Sub-Saharan Africa, a region with the greatest species diversity. Our results provide new insight into the taxonomy of Glinus in this region. Glinus lotoides var. virens accepted in many previous works is a synonym of G. dictamnoides that is closely related to G. lotoides based on molecular analysis and morphological characters. The status of the American populations of G. lotoides needs further investigation due to different characters of the specimens from the Old and the New World. Many specimens previously identified as G. lotoides var. virens and as the intermediates G. lotoides × G. oppositifolius belong to G. zambesiacus sp. nov. and G. hirtus comb. nov. (≡ Mollugo hirta); the latter species is resurrected from synonymy after 200 years of unacceptance. In some African treatments, G. hirtus was known under the invalidly published name G. dahomensis. Glinus zambesiacus is distributed in the southern and eastern parts of tropical Africa, and G. hirtus previously assumed to be endemic to West Africa is indeed a species with a wide distribution across the tropical part of the continent. Glinus microphyllus previously accepted as endemic to West Tropical Africa together with other new synonyms (G. oppositifolius var. lanatus, G. herniarioides, Wycliffea rotundifolia) is considered here as G. oppositifolius var. keenaniicomb. nov. (≡ Mollugo hirta var. keenanii), a variety found across the entire distribution of G. oppositifolius (Australia, Asia, and Africa). The presence of the American G. radiatus in Africa is not confirmed, and all records of this species belong to G. hirtus. The lectotypes of some names (G. dictamnoides, G. herniarioides, Mollugo hirta, M. setiflora, Pharnaceum pentagynum, Wycliffea) as well as a neotype of G. trianthemoides are designated. A new key to the identification of all Glinus species in Sub-Saharan Africa is provided. A checklist is given of all accepted species in this region (G. bainesii, G. hirtus, G. lotoides, G. oppositifolius s.l., G. setiflorus, and G. zambesiacus) with their nomenclature, morphological description and geographical distribution.

Biogeography of the xerophytic genus Anabasis L. (Chenopodiaceae).

AIM: Using the extremophile genus Anabasis, which includes c. 28 succulent, xerophytic C4 species, and is widely distributed in arid regions of Northern Africa, Arabia, and Asia, we investigate biogeographical relationships between the Irano-Turanian floristic region (ITfr) and its neighboring regions. We test whether the spread of arid and semi-arid biomes in Eurasia coincides with the biogeography of this drought-adapted genus, and whether the ITfr acted as source area of floristic elements for adjacent regions. LOCATION: Deserts and semi-deserts of Northern Africa, Mediterranean, Arabia, West and Central Asia. METHODS: Four cpDNA markers (rpL16 intron, atpB-rbcL, trnQ-rps16, and ndhF-rpL32 spacers) were sequenced for 58 accessions representing 21 Anabasis species. Phylogenetic relationships and divergence times were inferred using maximum likelihood and a time-calibrated Bayesian approach. To document the extant distribution of Anabasis, material from 23 herbaria was surveyed resulting in 441 well-documented collections used for the coding of eight floristic regions. Using this coded data, ancestral range was estimated using "BioGeoBEARS" under the DEC model. RESULTS: Anabasis originated during the Late Miocene and the ancestral range was probably widespread and disjunct between Western Mediterranean and the Irano-Turanian regions. Diversification started with two divergence events at the Miocene/Pliocene boundary (5.1 and 4.5 mya) leading to Asian clade I with ITfr origin which is sister to a slightly younger Asian clade II, which originated in the Western ITfr, and a Mediterranean/North African clade with an origin in the Western Mediterranean. MAIN CONCLUSIONS: Anabasis did not follow aridification and continuously expanded its distribution area, in fact its probably wide ancestral distribution area seems to have been fragmented during the very Late Miocene and the remnant lineages then expanded into neighboring arid regions. This genus supports the role of the ITfr as source area for xerophytic elements in the Mediterranean and Central Asia.

Nothodissotis (Melastomataceae), a new genus from Atlantic Central Africa, including the new species N.alenensis from Equatorial Guinea.

Based on morphological and phylogenetic evidence, a new genus of Melastomataceae (Melastomateae), Nothodissotis Veranso-Libalah & G.Kadereit, gen. nov., is described from Atlantic Central Africa. Nothodissotis is distinguished from other African Melastomateae genera by its calyx-lobes that are notched at apex and asymmetrical (vs. entire and symmetrical). Nothodissotis includes two species: the type species N.barteri (Hook.f.) Veranso-Libalah & G.Kadereit, comb. nov. (syn. Dissotisbarteri Hook.f.), and the new species N.alenensis Veranso-Libalah & O. Lachenaud, sp. nov., described and illustrated here. Both species are restricted to open vegetation on rock outcrops within the forested region of Atlantic Central Africa. Nothodissotisbarteri has a scattered distribution in Cameroon, Equatorial Guinea, Gabon and Príncipe Island, while N.alenensis is endemic to the Monte Alén massif in Equatorial Guinea, an area where N.barteri does not occur. Nothodissotisalenensis differs from N.barteri by its hypanthium bearing sessile appendages with penicillate hairs (vs. stalked stellate appendages) and its staminal appendages that are much smaller in antepetalous than in antesepalous stamens (vs. subequal in all stamens). The conservation status of both N.barteri and N.alenensis is assessed as Vulnerable in accordance with IUCN criteria.

Argyrella richardsiae, a new species of Melastomataceae from the wet miombo woodlands of south-central Africa.

A new species from the wet miombo woodlands of Tanzania and Angola, Argyrella richardsiae Veranso-Libalah & G.Kadereit, sp. nov. (Melastomataceae, Melastomateae), is described and illustrated. Although the widespread Argyrella canescens also occurs in Tanzania and northeastern Angola, A. richardsiae is morphologically most similar to Argyrella bambutorum known only from the Northwest of Cameroon, but differs by its indumentum of glandular trichomes on the entire plant (versus a mixture of stellate and glandular trichomes in other species of Argyrella), leaf-blades with serrulate margins (versus entire margins in A. bambutorum) and lateral nerves that become faint mid-way and never reach the leaf apex (versus conspicuous lateral nerves percurrent from the base to the apex in A. bambutorum). A preliminary conservation status of Endangered (EN) is proposed for A. richardsiae following the IUCN Red List Categories and Criteria. A taxonomic key and distribution map of all Argyrella species is also included.