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1.
Kidney Int ; 77(9): 771-80, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20164824

RESUMO

Reperfusion following ischemia is associated with acute kidney injury and inflammation. Using a mouse model, we exposed the kidney to a nonlethal period of ischemia, rendering it refractory to future ischemia-induced dysfunction. This ischemic preconditioning is partially mediated by Treg lymphocytes that suppress immune responses. We found that this maneuver significantly inhibited the accumulation of neutrophils and macrophages, tubular necrosis, and loss of kidney function caused by a subsequent ischemia/reperfusion injury 1 week later. The initial ischemia/reperfusion caused a significant increase in CD4(+)CD25(+)FoxP3(+) and CD4(+)CD25(+)IL-10(+) Treg cells within the kidney at 7 days of reperfusion. Treatment of preconditioned mice with a Treg cell-depleting antibody (PC61) reversed the effect of preconditioning on kidney neutrophil accumulation and partially inhibited the functional and histological protection of preconditioning. Adoptive transfer of Treg cells in naive mice, before ischemia/reperfusion, mimicked the protective and anti-inflammatory effects of ischemic preconditioning on the kidney. These studies highlight the role of Treg cells in ischemic preconditioning.


Assuntos
Precondicionamento Isquêmico/métodos , Linfócitos T Reguladores/imunologia , Injúria Renal Aguda/complicações , Injúria Renal Aguda/imunologia , Injúria Renal Aguda/prevenção & controle , Transferência Adotiva , Animais , Inflamação/complicações , Inflamação/imunologia , Interleucina-10/imunologia , Isquemia/complicações , Isquemia/imunologia , Isquemia/patologia , Rim/imunologia , Rim/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neutrófilos/imunologia , Neutrófilos/patologia , Traumatismo por Reperfusão/imunologia , Traumatismo por Reperfusão/patologia , Traumatismo por Reperfusão/prevenção & controle , Linfócitos T Reguladores/patologia
2.
J Am Soc Nephrol ; 20(8): 1744-53, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19497969

RESUMO

Both innate and adaptive mechanisms participate in the pathogenesis of kidney ischemia-reperfusion injury (IRI), but the role of regulatory immune mechanisms is unknown. We hypothesized that the anti-inflammatory effects of CD4(+)CD25(+)FoxP3(+) regulatory T cells (Tregs) protect against renal IRI. Partial depletion of Tregs with an anti-CD25 mAb potentiated kidney damage induced by IRI. Reducing the number of Tregs resulted in more neutrophils, macrophages, and innate cytokine transcription in the kidney after IRI but did not affect CD4(+) T cells or B cells. We performed adoptive transfer of lymph node cells from wild-type mice or FoxP3-deficient Scurfy mice into T cell- and B cell-deficient RAG-1 knockout mice to generate mice with and without FoxP3(+) Tregs, respectively. FoxP3(+) Treg-deficient mice accumulated a greater number of inflammatory leukocytes after renal IRI than mice containing Tregs. To confirm that a lack of Tregs potentiated renal injury, we co-transferred isolated Tregs and Scurfy lymph node cells; Treg repletion significantly attenuated IRI-induced renal injury and leukocyte accumulation. Furthermore, although adoptive transfer of wild-type Tregs into RAG-1 knockout mice was sufficient to prevent kidney IRI, transfer of IL-10-deficient Tregs was not. Taken together, these results demonstrate that Tregs modulate injury after kidney IRI through IL-10-mediated suppression of the innate immune system.


Assuntos
Imunidade Inata , Interleucina-10/metabolismo , Nefropatias/imunologia , Traumatismo por Reperfusão/imunologia , Linfócitos T Reguladores/fisiologia , Transferência Adotiva , Animais , Subunidade alfa de Receptor de Interleucina-2/imunologia , Nefropatias/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Traumatismo por Reperfusão/metabolismo
3.
Kidney Int ; 75(7): 689-98, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19129795

RESUMO

During renal ischemia-reperfusion, local and distant tissue injury is caused by an influx of neutrophils into the affected tissues. Here we measured the kinetics of margination and transmigration of neutrophils in vivo in the kidney and lungs following renal ischemia-reperfusion. After bilateral renal injury, kidney neutrophil content increased threefold at 24 h. The neutrophils were found primarily in the interstitium and to a lesser degree marginated to the vascular endothelium. These interstitial neutrophils had significantly lower levels of intracellular IFN-gamma, IL-4, IL-6, and IL-10 a tendency for decreased amounts of IL-4 and TNF-alpha compared to the marginated neutrophils. Localization of the neutrophils to the kidney interstitium was confirmed by high resolution microscopy and these sites of transmigration were directly associated with areas of increased vascular permeability. Activation of the adenosine 2A receptor significantly decreased both kidney neutrophil transmigration by about half and vascular permeability by about a third. After unilateral renal ischemia-reperfusion, the unclipped kidney and lungs did not accumulate interstitial neutrophils or have increased vascular permeability despite a marked increase of neutrophil margination in the lungs. Our findings suggest there is a sequential recruitment and transmigration of neutrophils from the vasculature into the kidney interstitium at the site of tissue injury following renal ischemia-reperfusion.


Assuntos
Nefropatias/imunologia , Pneumopatias/imunologia , Infiltração de Neutrófilos , Traumatismo por Reperfusão/imunologia , Animais , Permeabilidade Capilar , Citocinas/análise , Endotélio Vascular , Líquido Extracelular , Rim , Cinética , Camundongos , Camundongos Endogâmicos C57BL , Receptor A2A de Adenosina/metabolismo
4.
Kidney Int ; 75(2): 167-75, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18971925

RESUMO

Sphingosine-1-phosphate (S1P), produced by sphingosine kinase 1 (SphK1) or kinase 2 (SphK2), mediates biological effects through intracellular and/or extracellular mechanisms. Here we determined a role for these kinases in kidney injury of wild-type mice following ischemia-reperfusion. SphK1 but not SphK2 mRNA expression and activity increased in the kidney following injury relative to sham-operated animals. Although SphK1(-/-) mice had no alteration in renal function following injury, mice with a disrupted SphK2 gene (SphK2(tr/tr)) had histological damage and impaired function. The immune-modulating pro-drug, FTY720, an S1P agonist failed to provide protection in SphK2(tr/tr) mice. Injured kidneys of these mice showed increased neutrophil infiltration and neutrophil chemokine expression along with a 3- to 5-fold increase in expression of the G-protein-coupled receptor S1P(3) compared to heterozygous SphK2(+/tr) mice. Kidney function and reduced vascular permeability were preserved in S1P(3)(-/-) compared to S1P(3)(+/-) mice after ischemia-reperfusion injury, suggesting increased S1P(3) mRNA may play a role in the injury of SphK2(tr/tr) mice. Our study suggests that constitutive expression of SphK2 may contribute to reduced ischemia-reperfusion injury of the kidney, and its absence may enhance injury due to increased neutrophil infiltration and S1P(3) activation. We also confirm that SphK2 is necessary to mediate the protective effects of FTY720.


Assuntos
Nefropatias/enzimologia , Fosfotransferases (Aceptor do Grupo Álcool)/fisiologia , Traumatismo por Reperfusão/enzimologia , Animais , Cloridrato de Fingolimode , Regulação Enzimológica da Expressão Gênica , Rim/enzimologia , Rim/patologia , Nefropatias/patologia , Lisofosfolipídeos , Camundongos , Camundongos Knockout , Propilenoglicóis/farmacologia , RNA Mensageiro/análise , Traumatismo por Reperfusão/imunologia , Esfingosina/análogos & derivados , Esfingosina/farmacologia
5.
J Am Soc Nephrol ; 19(1): 59-68, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18045850

RESUMO

Adenosine 2A receptor (A(2A)R) activation was recently shown to be renoprotective in diabetic nephropathy. A(2A)R are found in glomeruli and have been shown to associate with the podocyte cytoskeletal protein alpha-actinin-4, but the effect of their activation on podocyte structure and function is unknown. Podocyte injury was induced in C57BL/6 mice with puromycin aminonucleoside, and the selective A(2A)R agonist ATL313 was found to attenuate the resulting albuminuria and foot process fusion. The selective A(2A)R antagonist ZM241385 reversed the effects of ATL313. In vitro, A(2A)R mRNA and protein were expressed in a conditionally immortalized podocyte cell line, and A(2A)R-like immunoreactivity co-localized with the actin cytoskeleton. Treatment with ATL313 also blocked the increased podocyte permeability to albumin and disruption of the actin cytoskeleton that accompanied puromycin aminonucleoside-induced injury in vitro. ATL313 was ineffective, however, in the presence of the A(2A)R antagonist and in A(2A)R-deficient podocytes. It was concluded that A(2A)R activation reduces glomerular proteinuria, at least in part, by preserving the normal structure of podocyte foot processes, slit diaphragms, and actin cytoskeleton.


Assuntos
Albuminúria/prevenção & controle , Podócitos/fisiologia , Receptores A2 de Adenosina/fisiologia , Agonistas do Receptor A2 de Adenosina , Animais , Nefropatias Diabéticas/fisiopatologia , Nefropatias Diabéticas/prevenção & controle , Camundongos , Camundongos Endogâmicos C57BL , Piperidinas/uso terapêutico , Podócitos/efeitos dos fármacos , Proteinúria/induzido quimicamente , Proteinúria/prevenção & controle , Nucleosídeos de Purina/efeitos adversos , Receptores A2 de Adenosina/efeitos dos fármacos , Triazinas/farmacologia , Triazinas/uso terapêutico , Triazóis/farmacologia , Triazóis/uso terapêutico
6.
Kidney Int ; 74(12): 1526-37, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18843253

RESUMO

Chemokines and their receptors such as CCR2 and CX3CR1 mediate leukocyte adhesion and migration into injured tissue. To further define mechanisms of monocyte trafficking during kidney injury we identified two groups of F4/80-positive cells (F4/80(low) and F4/80(high)) in the normal mouse kidney that phenotypically correspond to macrophages and dendritic cells, respectively. Following ischemia and 3 h of reperfusion, there was a large influx of F4/80(low) inflamed monocytes, but not dendritic cells, into the kidney. These monocytes produced TNF-alpha, IL-6, IL-1alpha and IL-12. Ischemic injury induced in CCR2(-/-) mice or in CCR2(+/+) mice, made chimeric with CCR2(-/-) bone marrow, resulted in lower plasma creatinine levels and their kidneys had fewer infiltrated F4/80(low) macrophages compared to control mice. CX3CR1 expression contributed to monocyte recruitment into inflamed kidneys, as ischemic injury in CX3CR1(-/-) mice was reduced, with fewer F4/80(low) macrophages than controls. Monocytes transferred from CCR2(+/+) or CX3CR1(+/-) mice migrated into reperfused kidneys better than monocytes from either CCR2(-/-) or CX3CR1(-/-) mice. Adoptive transfer of monocytes from CCR2(+/+) mice, but not CCR2(-/-) mice, reversed the protective effect in CCR2(-/-) mice following ischemia-reperfusion. Egress of CD11b(+)Ly6C(high) monocytes from blood into inflamed kidneys was CCR2- and CX3CR1-dependent. Our study shows that inflamed monocyte migration, through CCR2- and CX3CR1-dependent mechanisms, plays a critical role in kidney injury following ischemia reperfusion.


Assuntos
Quimiotaxia/imunologia , Nefropatias/imunologia , Receptores CCR2/fisiologia , Receptores de Quimiocinas/fisiologia , Traumatismo por Reperfusão/imunologia , Transferência Adotiva , Animais , Receptor 1 de Quimiocina CX3C , Isquemia , Macrófagos/fisiologia , Camundongos , Camundongos Knockout , Monócitos/fisiologia
7.
J Clin Invest ; 120(1): 331-42, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20038794

RESUMO

The IL-23/IL-17 and IL-12/IFN-gamma cytokine pathways have a role in chronic autoimmunity, which is considered mainly a dysfunction of adaptive immunity. The extent to which they contribute to innate immunity is, however, unknown. We used a mouse model of acute kidney ischemia-reperfusion injury (IRI) to test the hypothesis that early production of IL-23 and IL-12 following IRI activates downstream IL-17 and IFN-gamma signaling pathways and promotes kidney inflammation. Deficiency in IL-23, IL-17A, or IL-17 receptor (IL-17R) and mAb neutralization of CXCR2, the p19 subunit of IL-23, or IL-17A attenuated neutrophil infiltration in acute kidney IRI in mice. We further demonstrate that IL-17A produced by GR-1+ neutrophils was critical for kidney IRI in mice. Activation of the IL-12/IFN-gamma pathway and NKT cells by administering alpha-galactosylceramide-primed bone marrow-derived DCs increased IFN-gamma production following moderate IRI in WT mice but did not exacerbate injury or enhance IFN-gamma production in either Il17a-/- or Il17r-/- mice, which suggested that IL-17 signaling was proximal to IFN-gamma signaling. This was confirmed by the finding that IFN-gamma administration reversed the protection seen in Il17a-/- mice subjected to IRI, whereas IL-17A failed to reverse protection in Ifng-/- mice. These results demonstrate that the innate immune component of kidney IRI requires dual activation of the IL-12/IFN-gamma and IL-23/IL-17 signaling pathways and that neutrophil production of IL-17A is upstream of IL-12/IFN-gamma. These mechanisms might contribute to reperfusion injury in other organs.


Assuntos
Interferon gama/fisiologia , Interleucina-17/fisiologia , Rim/irrigação sanguínea , Neutrófilos/fisiologia , Traumatismo por Reperfusão/imunologia , Animais , Movimento Celular , Imunidade Inata , Inflamação/etiologia , Interleucina-12/fisiologia , Interleucina-23/fisiologia , Células Matadoras Naturais/fisiologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos C57BL , Receptores de Quimiocinas/fisiologia , Traumatismo por Reperfusão/etiologia , Transdução de Sinais
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