Thorough Validation of Optimized Size Exclusion Chromatography-Total Organic Carbon Analysis for Natural Organic Matter in Fresh Waters.

Size exclusion chromatography with total organic carbon detection (HPSEC-TOC) is a widely employed technique for characterizing aquatic natural organic matter (NOM) into high, medium, and low molecular weight fractions. This study validates the suitability of HPSEC-TOC for a simplified yet efficient routine analysis of freshwater and its application within drinking water treatment plants. The investigation highlights key procedural considerations for optimal results and shows the importance of sample preservation by refrigeration with a maximum storage duration of two weeks. Prior to analysis, the removal of inorganic carbon is essential, which is achieved without altering the NOM composition through sample acidification to pH 6 and subsequent N2-purging. The chromatographic separation employs a preparative TSK HW-50S column to achieve a limit of detection of 19.0 µgC dm-3 with an injection volume of 1350 mm-3. The method demonstrates linearity up to 10,000 µgC dm-3. Precision, trueness and recovery assessments are conducted using certified reference materials, model compounds, and real water samples. The relative measurement uncertainty in routine analysis ranges from 3.22% to 5.17%, while the measurement uncertainty on the bias is 8.73%. Overall, the HPSEC-TOC represents a reliable tool for NOM fractions analysis in both treated and untreated ground and surface water.

Effectiveness of terracing techniques for controlling soil erosion by water in Rwanda.

Despite long-standing efforts in terracing, limited field-based evidence of its effectiveness as implemented within rural farming systems of humid tropical regions, such as Rwanda, is available. This study aimed to reveal regional differences in effectiveness of two widely used terracing techniques. Traditional slope farming (NP) was compared to bench (BT) and farmers' based progressive terraces (PT) in terms of runoff, soil losses, and topsoil fertility in two contrasting agro-ecological zones, the Eastern Plateau (Murehe) and Buberuka Highlands (Tangata). During four consecutive rainy seasons, event-based data were collected using erosion plots (5 m width x 22.2 m length). Effectiveness indices of both terracing systems, as well as (R)USLE P-factor values, were calculated. The annual average soil losses under NP ranged from 4.71 ± 5.02 ton ha-1 to 46.01 ± 7.28 ton ha-1 in Murehe (14% slope gradient) and Tangata (43% slope gradient), respectively. Bench terracing clearly outperformed the farmer-based progressive terrace at both locations, leading to negligible soil losses. In terms of runoff reduction, an effectiveness of 70 and 85% respectively, was observed at Murehe and Tangata. The effectiveness of PT reached 52% for runoff control and 93% for soil loss control at Tangata, thereby confirming its huge potential as erosion control measure, even in mountainous areas. In the hilly landscape of Murehe, the runoff generated by PT - in some years - can exceed that under traditional farming, while the measure reduced soil losses by half on average. Associated USLE P-factors varied between seasons with an annual average values of 0.001-0.02 for BT, and 0.07 to 0.55 for PT at Tangata and Murehe, respectively. These variations in performance by site and terracing system also resulted in differences in topsoil chemical fertility, with BT generally outperforming both PT and NP at Tangata. At Murehe, PT showed a significantly lower chemical fertility compared to BT and NP. Poor quality risers explained the overall lower performance of PT at Murehe. The study thus confirmed the huge potential of (bench) terraces to sustainably reduce soil erosion rates when established within an integrated approach, paying attention to correct installation and fertility-supporting agronomic practices. More attention should be given to riser installation (e.g. distance) and maintenance of PT. Adoption of these erosion control measures can be recommended to similar agro-ecological zones for sustainably protecting the lands while mitigating or adapting the effects of climate change.

Comparison of quantitative mineralogy and sequential leaching for characterization of Ni in workplace dust collected at a stainless steel operation.

Based on epidemiological records of workers at Ni operations, regulatory guidelines commonly target specific Ni compounds for setting exposure limits. Thus, reliable methods of Ni speciation in airborne dust samples are required for effective monitoring of workplace exposure. Zatka sequential leaching has been routinely performed industry-wide since the 1990s for characterization of Ni in dust samples; however, limitations related to leaching kinetics have been identified, and optimization of the methodology is required to improve accuracy of data. In this study, Ni characterization of dust collected from a stainless steel operation was performed using Zatka sequential leaching (original and modified protocols) and quantitative mineralogy (QEMSCAN), a method novel to the field of industrial hygiene. Mineral analysis was also performed on bulk material collected from selected work areas at the plant. The results are compared with the objective of identifying opportunities to optimize the methods for characterizing dust that is unique to stainless steel manufacturing. The quantitative mineralogical analysis determined that the Ni dust is composed of oxidic Ni (chromite and trevorite, >80% of the Ni in most samples) and metallic Ni (Ni-Fe alloy), and the results were validated against chemical assays and alternate methods of mineral characterization. In contrast, the original Zatka method erroneously identified soluble Ni as a major Ni contributor, whereas the modified Zatka method identified sulfidic Ni. The mineralogy identified Ni-barren dust and grain sizes and liberation of individual Ni compounds as potential factors that can affect leaching selectivity. Clearly, for any sequential leaching method to be useful for these workplaces, they should be optimized by including reference materials that are representative of Ni substances present at stainless steel operations (chromite, trevorite, and Ni-Fe alloy). Improving methods of sequential leaching is important because the resolution of quantitative mineralogical techniques diminishes at <3 µm (respirable dust fraction). We recommend that quantitative mineralogy be performed in parallel with methods of sequential leaching to provide a robust system of characterization.

Century-long apparent decrease in intrinsic water-use efficiency with no evidence of progressive nutrient limitation in African tropical forests.

Forests exhibit leaf- and ecosystem-level responses to environmental changes. Specifically, rising carbon dioxide (CO2 ) levels over the past century are expected to have increased the intrinsic water-use efficiency (iWUE) of tropical trees while the ecosystem is gradually pushed into progressive nutrient limitation. Due to the long-term character of these changes, however, observational datasets to validate both paradigms are limited in space and time. In this study, we used a unique herbarium record to go back nearly a century and show that despite the rise in CO2 concentrations, iWUE has decreased in central African tropical trees in the Congo Basin. Although we find evidence that points to leaf-level adaptation to increasing CO2 -that is, increasing photosynthesis-related nutrients and decreasing maximum stomatal conductance, a decrease in leaf δ13 C clearly indicates a decreasing iWUE over time. Additionally, the stoichiometric carbon to nitrogen and nitrogen to phosphorus ratios in the leaves show no sign of progressive nutrient limitation as they have remained constant since 1938, which suggests that nutrients have not increasingly limited productivity in this biome. Altogether, the data suggest that other environmental factors, such as increasing temperature, might have negatively affected net photosynthesis and consequently downregulated the iWUE. Results from this study reveal that the second largest tropical forest on Earth has responded differently to recent environmental changes than expected, highlighting the need for further on-ground monitoring in the Congo Basin.

Uncovering the biofumigant capacity of allyl isothiocyanate from several Brassicaceae crops against Fusarium pathogens in maize.

BACKGROUND: Driven by environmental concerns, chemical fumigants are no longer allowed in many countries. Therefore, other strategies for reducing fungal inoculum in soils and on crop debris are being explored. In the present study, several Brassicaceae crops were screened for their potential to control Fusarium gramineaum and Fusarium poae mycelial growth in an in vitro inverted Petri dish experiment. Volatile production was measured using gas chromatography-mass spectrometry headspace analysis. A selection of cultivars from each crop species was further investigated using a pot experiment with maize. RESULTS: Ethiopian mustard (Brassica carinata) and brown mustard (Brassica juncea) released volatile allyl isothiocyanate (AITC) and a higher concentration of AITC was correlated with a better fungal growth reduction in the in vitro screening. Brown mustard cultivar Etamine completely inhibited growth of both Fusarium spp. Pure AITC in a solution with methanol resulted in a sigmoid dose-response curve for both Fusarium spp. tested. Fusarium poae appeared to be more tolerant to AITC than F. graminearum. A pot experiment revealed that the incorporation of brown mustard plant material could alleviate the clear negative effect of F. graminearum infection on maize growth. CONCLUSION: The present study demonstrated the correlation between the fungistatic effect of biofumigation crops on Fusarium spp. and their production of volatile AITC in vitro, without the addition of exogenous enzymes, and confirmed the biofumigation potential of brown mustard in a pot experiment with maize. These results may help farmers when selecting a green manure crop suitable for biofumigation. © 2020 Society of Chemical Industry.

In vitro mycorrhization of pear (Pyrus communis).

The Mycelium Donor Plant system (MDP) was adapted to study the time course of the colonization of Pyrus communis by Rhizophagus irregularis under in vitro conditions. Isolated germinated spores did not colonize pear roots. Inoculum composed of R. irregularis spores/mycelium associated with chicory root fragments was used to inoculate Medicago truncatula which became thereafter the MDP of pear plantlets. Typical intraradical structures (hyphae, arbuscules, spores/vesicles) and appressoria were observed in the pear roots. During acclimatization, the pear plants formed a densely branched root system. R. irregularis colonization not only altered the root architecture but also changed the nutrient composition of the acclimatized pear plantlets.

Effect of zinc oxide sources and dosages on gut microbiota and integrity of weaned piglets.

Zinc oxide (ZnO) supplied at pharmacological dosage in diets of weaned piglets improves growth performance. However, it causes environmental contamination and induces bacterial antibiotic resistance, yet this practice is debated. The effects on gut microbiota and integrity in weaned piglets of conventional ZnO at nutritional and pharmacological dosage (110 and 2,400 mg/kg Zn, respectively) were compared to an alternative ZnO source at 110 and 220 mg/kg Zn. Each of the four treatments was applied to four pens (two piglets/pen; weaning age, 20 days) for 15 days, and piglets were sampled on day 15 to determine indices of gut integrity. Feeding conventional ZnO at 2,400 mg/kg Zn reduced coliforms and Escherichia coli in distal small intestine as compared to conventional ZnO at 110 mg/kg (-1.7 and -1.4 log10 cfu/g, respectively), whereas the alternative ZnO reduced only coliforms, irrespective of dosage (-1.6 to -1.7 log10 cfu/g). Transepithelial electrical resistance of distal small intestinal mucosa was higher for pigs fed the alternative ZnO source as compared with groups fed 110 mg/kg Zn of conventional ZnO, in line with a trend for higher gene expression of claudin-1 and zona occludens-1. Interestingly, the alternative ZnO source at 110 and 220 mg/kg Zn increased intestinal alkaline phosphatase gene transcript as compared to conventional ZnO at 110 mg/kg Zn, whereas the alternative ZnO source at 110 mg/kg Zn exhibited higher Zn concentrations in mucosa (2,520 µg/g) as compared to conventional ZnO at 110 mg/kg Zn (1,211 µg/g). However, assessing alkaline phosphatase activity, no significant effects were found. In conclusion, the alternative ZnO reduced digesta Enterobacteriaceae numbers and improved gut integrity, albeit similar or better, depending on the dosage, to the effects of pharmacological dosage of conventional ZnO.

Influence of aqueous precursor chemistry on the growth process of epitaxial SrTiO3 buffer layers.

In this Article, epitaxial thin films of SrTiO3 were prepared on single crystalline (100) LaAlO3 by an aqueous chemical solution deposition method. By using different chelating agents to stabilize the metal ions in water, the impact of the precursor chemistry on the microstructural and crystalline properties of the films was studied. Thorough investigation of the precursor by means of infrared and Raman spectroscopy as well as thermogravimetric analysis revealed that stable precursors can be obtained in which strontium ions can be either free in the solution or stabilized by one of the chelating agents. This stabilization of strontium ions appeared to be essential in order to obtain single phase SrTiO3 films. Precursors in which Sr(2+) remained as free ions showed SrO microcrystal segregation. Precursors in which both metal ions were stabilized gave rise to strongly textured, dense, and terraced SrTiO3 films, allowing subsequent deposition of YBa2Cu3O7-δ with superior superconducting performances.

Effects of Genotype, Nitrogen, and Sulfur Complex Fertilization on the Nutritional and Technological Characteristics of Buckwheat Flour.

The present study investigated the effect of nitrogen fertilization (NF) at the levels of 0, 45, and 90 kg·ha-1 combined with selected sulfur complex fertilization (SCF) levels of 0 and 45 kg·ha-1 on the nutritional and technological characteristics of buckwheat flour from five varieties. The results showed that the genotype was a critical factor affecting the chemical composition and physicochemical properties of buckwheat flour. NF significantly increased protein, total starch, and amylose content as well as mineral composition but decreased particle size, color value, and water hydration properties. However, SCF enhanced the ash content and decreased the protein content but had no significant effect on the pasting temperature. In addition, the combination of NF and SCF significantly reduced granule size, water solubility, viscosity, and rheological properties with increasing fertilization levels. This study can guide the cultivation of buckwheat with the desired physicochemical properties and provide information for buckwheat-based products in the food industry.

Combined effects of nitrogen and sulfur fertilizers on chemical composition, structure and physicochemical properties of buckwheat starch.

Buckwheat starch has attracted worldwide attention in the food industry as a valuable raw material or food additive. Nitrogen (N) and sulfur (S) are two nutrients essential to ensure grain quality. This study investigated the combined application of N fertilizer (0, 45 and 90 kg N ha-1) and S fertilizer (0 and 45 kg SO3 ha-1) on the chemical composition, structure and physicochemical properties of buckwheat starch. The results showed that increasing the fertilizer application decreased amylose content and starch granule size but increased light transmittance, water solubility and swelling power. The stability of the absorption peak positions and the decrease in short-range order degree suggested that fertilization influenced the molecular structure of buckwheat starch. In addition, increases in viscosity and gelatinization enthalpy as well as decreases in gelatinization temperatures and dynamic rheological properties indicated changes in the processing characteristics and product quality of buckwheat-based foods.

Influence of calcium concentration on the re-assembly of sodium caseinate into casein micelles and on their renneting behavior.

Inducing the spontaneous aggregation from casein molecules (i.e. αs1, αs2, ß, and κ-casein) into re-assembled casein micelles (RCMs) through the addition of salts as an alternative to native casein micelles, has garnered increasing attention in recent years. In this investigation, re-assembled casein micelles were generated by adding varying amounts of calcium, phosphate, and citrate ions to a sodium caseinate dispersion. The formed micelles were further characterized in terms of particle size, optical density, and partitioning of calcium ions and caseins. Besides, their small-angle X-ray scattering (SAXS) profiles and renneting properties were evaluated. The observations revealed that the particle size and optical density of RCMs increased with the continuous addition of salts, while the micellar yield improved and could exceed 85 %. Moreover, the quantity of individual casein molecules that contributed to the creation of micelles was in concordance with their level of phosphorylation (i.e. αs2-casein > αs1-casein > ß-casein > κ-casein). Mineral analysis results and SAXS scattering profiles confirmed that the added calcium ions acted as cross-linkers and participated in the construction of calcium phosphate nanoclusters. The renneting ability of RCMs was primarily dependent upon the colloidal calcium content per gram of micellar casein.

The Impact of 10 Unmalted Alternative Adjuncts on Wort Characteristics.

Consumers are more than ever in search of novel and exciting beer choices, and brewers are, therefore, continuously experimenting to adapt their product portfolio. One interesting way to naturally incorporate novel flavors and tastes is by using alternative adjuncts, but this is not always an easy and straightforward process. In this study, a 40% unmalted alternative adjunct (einkorn, emmer, spelt, khorasan, quinoa, amaranth, buckwheat, sorghum, teff, and tritordeum) or reference (barley malt, unmalted barley, and unmalted wheat) was added to 60% barley malt, after which three different laboratory mashing processes (Congress mash, Congress mash with pre-gelatinization of the adjunct, and Evans mash) were performed, and their behavior during mashing and the resulting wort characteristics were investigated in detail. Overall, the extraction process of all 10 unmalted alternative adjuncts was not complete for all three laboratory mashing processes, whereby Congress mashing resulted in the highest extract and fastest filtration, whereas Evans mashing resulted in the lowest extract and slowest filtration. Pre-gelatinization of the unmalted was generally only beneficial for adjuncts with high onset starch gelatinization temperatures. This process also inactivated endogenous enzymes in the unmalted adjuncts, which had an adverse effect on the mashing process.

Variation in potential feeding value of triticale forage among plant fraction, maturity stage, growing season and genotype.

Cereal forages, such as triticale forage, progressively gain interest as alternative crop for maize. The main study objective was to investigate the variation in potential feeding value of triticale forage among maturity stage, growing season and genotype, using total plant and stem fractions. Therefore, near infrared spectroscopy (NIRS) was evaluated as fast screening tool. The prediction ability was good (ratio of prediction to deviation, RPD ≥3.0) for total plant residual moisture, starch, sugars and for stem crude ash (CAsh) and neutral detergent fibre (aNDFom); suitable for screening (2.0 ≤ RPD <3.0) for total plant CAsh, acid detergent fibre (ADFom), in vitro digestibility of organic matter (IVOMD), in vitro digestibility of neutral detergent fibre (IVNDFD) and for stem total lignin (TL) and IVNDFD; poor (1.5 ≤ RPD <2.0) for total plant crude protein, crude fat, aNDFom, lignin (sa) and for stem Klason lignin (KL); unreliable (RPD <1.5) for stem residual moisture and acid soluble lignin (ASL). The evolution in potential feeding value of 36 genotypes harvested at the medium and late milk to the early, soft and hard dough stage was followed. The most important changes occurred between the late milk and early dough stage, with little variation in quality after the soft dough stage. During 2 growing seasons, variation in feeding value of 120 genotypes harvested at the soft dough stage was demonstrated. Interestingly, variation in stem IVNDFD is almost twice as high as for the total plant (CV 12.4% versus 6.6%). Furthermore, Spearman correlations show no link between dry matter yield and digestibility of genotypes harvested at the soft dough stage. Based on linear regression models ADFom appears as main predictor of both plant IVOMD and plant IVNDFD. Stem IVNDFD is particularly determined by KL.

Boosting aerobic microbial protein productivity and quality on brewery wastewater: Impact of anaerobic acidification, high-rate process and biomass age.

Consortia of aerobic heterotrophic bacteria (AHB) are appealing as sustainable alternative protein ingredient for aquaculture given their high nutritional qualities, and their production potential on feed-grade industrial wastewater. Today, the impacts of pre-treatment, bioprocess choice and key parameter settings on AHB productivity and nutritional properties are unknown. This study investigated for the first time AHB microbial protein production effects based on (i) raw vs anaerobically fermented brewery wastewater, (ii) high-rate activated sludge (HRAS) without vs with feast-famine conditions, and (iii) three short solid retention time (SRT): 0.25, 0.50 and 1.00 d. High biomass (4.4-8.0 g TSS/L/d) and protein productivities (1.9-3.2 g protein/L/d) were obtained while achieving COD removal efficiencies up to 98 % at SRT 0.50 d. The AHB essential amino acid (EAA) profiles were above rainbow trout requirements, excluding the S-containing EAA, highlighting the AHB biomass replacement potential for unsustainable fishmeal in salmonid diets.

Purple bacteria screening for photoautohydrogenotrophic food production: Are new H2-fed isolates faster and nutritionally better than photoheterotrophically obtained reference species?

Photoautohydrogenotrophic enrichments of wastewater treatment microbiomes were performed to obtain hypothetically high-potential specialist species for biotechnological applications. From these enrichment cultures, ten photoautohydrogenotrophic species were isolated: six Rhodopseudomonas species, three Rubrivivax members and Rhodobacter blasticus. The performance of these isolates was compared to three commonly studied, and originally photoheterotrophically enriched species (Rhodopseudomonas palustris, Rhodobacter capsulatus and Rhodobacter sphaeroides), designated as reference species. Repeated subcultivations were applied to improve the initial poor performance of the isolates (acclimation effect), which resulted in increases in both maximum growth rate and protein productivity. However, the maximum growth rate of the reference species remained 3-7 times higher compared to the isolates (0.42-0.84 d-1 at 28 °C), while protein productivities remained 1.5-1.7 times higher. This indicated that H2-based enrichment did not result in photoautohydrogenotrophic specialists, suggesting that the reference species are more suitable for intensified biomass and protein production. On the other hand, the isolates were able to provide equally high protein quality profiles as the references species, providing full dietary essential amino acid matches for human food. Lastly, the effect of metabolic carbon/electron switching (back and forth between auto- to heterotrophic conditions) initially boosted µmax when returning to photoautohydrogenotrophic conditions. However, the switch negatively impacted lag phase, protein productivities and pigment contents. In the case of protein productivity, the acquired acclimation was partially lost with decreases of up to 44 % and 40 % respectively for isolates and reference species. Finally, the three reference species, and specifically Rh. capsulatus, remained the most suitable candidate(s) for further biotechnological development.

Innovative Rhizosphere-Based Enrichment under P-Limitation Selects for Bacterial Isolates with High-Performance P-Solubilizing Traits.

The use of phosphate solubilizing bacteria (PSB) as inoculants for the rhizosphere is a well-known strategy to mitigate P-deficiency in plants. However, despite the multiple modes of action to render P available for plants, PSB often fail to deliver in the field as their selection is often based on a single P-solubilizing trait assessed in vitro. Anticipating these shortcomings, we screened 250 isolates originating from rhizosphere-based enriched consortia for the main in vitro P-solubilizing traits, and subsequently grouped the isolates through trait-based HCPC (hierarchical clustering on principal components). Representative isolates of each cluster were tested in an in planta experiment to compare their in vitro P-solubilizing traits with their in planta performance under conditions of P-deprivation. Our data convincingly show that bacterial consortia capable to mitigate P-deficiency in planta were enriched in bacterial isolates that had multiple P-solubilizing traits in vitro and that had the capacity to mitigate plant P-stress in planta under P-deprived conditions. Furthermore, although it was assumed that bacteria that looked promising in vitro would also have a positive effect in planta, our data show that this was not always the case. Opposite, lack of performance in vitro did not automatically result in a lack of performance in planta. These results corroborate the strength of the previously described in planta-based enrichment and selection technique for the isolation of highly efficient rhizosphere competent PSB. IMPORTANCE With the growing awareness on the ecological impact of chemical phosphate fertilizers, research concerning the use of phosphate solubilizing bacteria (PSB) as a sustainable alternative for, or addition to these fertilizers is of paramount importance. In previous research, we successfully implemented a plant-based enrichment technique for PSB, which simultaneously selected for the rhizosphere competence and phosphate solubilizing characteristics of bacterial suspensions. Current research follows up on our previous findings, whereas we screened 250 rhizobacteria for their P-solubilizing traits and were able to substantiate the results obtained from the enriched suspensions at a single-isolate level. With this research, we aim for a paradigm shift toward the plant-based selection of PSB, which is a more holistic approach compared to the plate-based methods. We emphasize the strength of the previously described plant-based enrichment and selection technique for the isolation of highly efficient and diverse PSB.

Geochemical fingerprinting and magnetic susceptibility to unravel the heterogeneous composition of urban soils.

The typically high heterogeneity of urban soil properties challenges their characterization and interpretation. The objective of this study was to investigate if proximally sensed volume-specific magnetic susceptibility and/or geochemical soil properties can uncover differences in anthropogenic, lithogenic and pedological contributions in, and between, urban soils. We also tested if volume-specific magnetic susceptibility can predict heavy metal enrichment. Data on 29 soil properties of 103 soil horizons from 16 soils from Ghent, Belgium, were analyzed by factor analysis. A correlation analysis, and in-depth analysis of five contrasting urban soils supplemented insights gained from the factor analysis. The factor analysis extracted four factors: 29.2 % of the soil property variability was attributed to fossil fuel combustion and industrial processes, with high (>0.80) loadings for S, organic carbon, magnetic susceptibility, and Zn. Furthermore, 26.0 % of the variability was linked to parent material differences, with high loadings (>0.80) for K, Rb and Ti. In absence of geogenic carbonates, increased soil alkalinity due to anthropogenic input of CaCO3 explained 17.0 % of the variability. Lastly, 4.7 % of the variability resulted from variable Zr contents by local geology. Elemental analysis by XRF, possibly combined with magnetic susceptibility measurements, helped to explain lateral or vertical differences related to (1) the nature of anthropogenic influence, for instance burning (e.g., by the S and Zn content) or the incorporation of building rubble (e.g., by the Ca content); (2) the particle size distribution (e.g., by the K, Rb or Ti content); (3) lithology (e.g., by the Zr content); or (4) pedology, such as organic matter build-up (e.g., by the S content) or leaching of alkalis (e.g., by the Ca content). Even though artifacts and soil translocation were common in the studied soils, volume-specific soil magnetic susceptibility measured on fine earth predicted the total heavy metal pollution loading index well (Pearson correlation = 0.85).

Aerobes and phototrophs as microbial organic fertilizers: Exploring mineralization, fertilization and plant protection features.

Organic fertilizers and especially microbial biomass, also known as microbial fertilizer, can enable a paradigm shift to the conventional fertilizer-to-food chain, particularly when produced on secondary resources. Microbial fertilizers are already common practice (e.g. Bloom® and Synagro); yet microbial fertilizer blends to align the nutrient release profile to the plant's needs are, thus far, unexplored. Moreover, most research only focuses on direct fertilization effects without considering added value properties, such as disease prevention. This study has explored three promising types of microbial fertilizers, namely dried biomass from a consortium of aerobic heterotrophic bacteria, a microalga (Arthrospira platensis) and a purple non-sulfur bacterium (Rhodobacter sphaeroides). Mineralization and nitrification experiments showed that the nitrogen mineralization profile can be tuned to the plant's needs by blending microbial fertilizers, without having toxic ammonium peaks. In a pot trial with perennial ryegrass (Lolium perenne L.), the performance of microbial fertilizers was similar to the reference organic fertilizer, with cumulative dry matter yields of 5.6-6.7 g per pot. This was confirmed in a pot trial with tomato (Solanum lycopersicum L.), showing an average total plant length of 90-99 cm after a growing period of 62 days for the reference organic fertilizer and the microbial fertilizers. Moreover, tomato plants artificially infected with powdery mildew (Oidium neolycopersici), a devastating disease for the horticultural industry, showed reduced disease symptoms when A. platensis was present in the growing medium. These findings strengthen the application potential of this novel class of organic fertilizers in the bioeconomy, with a promising match between nutrient mineralization and plant requirements as well as added value in crop protection.

Increasing calcium scarcity along Afrotropical forest succession.

Secondary forests constitute an increasingly important component of tropical forests worldwide. Although cycling of essential nutrients affects recovery trajectories of secondary forests, the effect of nutrient limitation on forest regrowth is poorly constrained. Here we use three lines of evidence from secondary forest succession sequences in central Africa to identify potential nutrient limitation in regrowing forests. First, we show that atmospheric phosphorus supply exceeds demand along forest succession, whereas forests rely on soil stocks to meet their base cation demands. Second, soil nutrient metrics indicate that available phosphorus increases along the succession, whereas available cations decrease. Finally, fine root, foliar and litter stoichiometry show that tissue calcium concentrations decline relative to those of nitrogen and phosphorus during succession. Taken together, these observations suggest that calcium becomes an increasingly scarce resource in central African forests during secondary succession. Furthermore, ecosystem calcium storage shifts from soil to woody biomass over succession, making it a vulnerable nutrient in the wake of land-use change scenarios that involve woody biomass export. Our results thus call for a broadened focus on elements other than nitrogen and phosphorus regarding tropical forest biogeochemical cycles and identify calcium as a scarce and potentially limiting nutrient in an increasingly disturbed and dynamic tropical forest landscape.

Microbial food from light, carbon dioxide and hydrogen gas: Kinetic, stoichiometric and nutritional potential of three purple bacteria.

The urgency for a protein transition towards more sustainable solutions is one of the major societal challenges. Microbial protein is one of the alternative routes, in which land- and fossil-free production should be targeted. The photohydrogenotrophic growth of purple bacteria, which builds on the H2- and CO2-economy, is unexplored for its microbial protein potential. The three tested species (Rhodobacter capsulatus, Rhodobacter sphaeroides and Rhodopseudomonas palustris) obtained promising growth rates (2.3-2.7 d-1 at 28°C) and protein productivities (0.09-0.12 g protein L-1 d-1), rendering them likely faster and more productive than microalgae. The achieved protein yields (2.6-2.9 g protein g-1 H2) transcended the ones of aerobic hydrogen oxidizing bacteria. Furthermore, all species provided full dietary protein matches for humans and their fatty acid content was dominated by vaccenic acid (82-86%). Given its kinetic and nutritional performance we recommend to consider Rhodobacter capsulatus as a high-potential sustainable source of microbial food.