RESUMO
BACKGROUND: Rapid identification and treatment of tissue hypoxia reaching anaerobiosis (dysoxia) may reduce organ failure and the occurrence of major postoperative complications (MPC) after cardiac surgery. The predictive ability of PCO2-based dysoxia biomarkers, central venous-to-arterial PCO2 difference (ΔPCO2) and ΔPCO2 to arteriovenous oxygen content difference ratio, is poorly studied in this setting. OBJECTIVES: We evaluated the ability of PCO2-based tissue dysoxia biomarkers, blood lactate concentration and central venous oxygen saturation measured 2âh after admission to the ICU as predictors of MPC. DESIGN: A prospective, observational cohort study. SETTING: Single-centre, academic hospital cardiovascular ICU. PATIENTS: We included adult patients undergoing cardiac surgery with cardiopulmonary bypass and measured dysoxia biomarkers at ICU admission, and after 2, 6 and 24âh. MAIN OUTCOME MEASURES: The primary endpoint was MPC, a composite of cardiac and noncardiac MPC evaluated in the 48âh following surgery. After univariate analysis of MPC covariates including dysoxia biomarkers measured at 2âh, multivariate logistic regression analyses were performed to identify the association of these biomarkers with MPC for confounders. Areas under the receiver operating characteristic curves were determined for biomarkers which remained independently associated with MPC. RESULTS: MPC occurred in 56.5% of the 308 patients analysed. ΔPCO2, blood lactate concentration and central venous oxygen saturation measured at 2âh, but not ΔPCO2 to arteriovenous oxygen content difference ratio, were significantly associated with MPC. However, only ΔPCO2 was independently associated with MPC after multivariate analysis. The areas under the receiver operating characteristic curves of ΔPCO2 measured at 2âh for MPC prediction was 0.64 (95% CI 0.57 to 0.70, Pâ<â0.001). CONCLUSION: After cardiac surgery with cardiopulmonary bypass, ΔPCO2 measured 2âh after ICU admission was the only dysoxia biomarker independently associated with MPC, but with limited performance. TRIAL REGISTRATION: ClinicalTrials.gov, NCT03107572.
Assuntos
Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Ponte Cardiopulmonar/efeitos adversos , Hipóxia/sangue , Complicações Pós-Operatórias/diagnóstico , Idoso , Biomarcadores/sangue , Gasometria , Dióxido de Carbono/sangue , Feminino , Humanos , Hipóxia/etiologia , Unidades de Terapia Intensiva , Masculino , Pessoa de Meia-Idade , Oxigênio/sangue , Complicações Pós-Operatórias/etiologia , Prognóstico , Estudos Prospectivos , Medição de Risco , Resultado do TratamentoRESUMO
OBJECTIVE: To investigate mutations in the D-loop region of mitochondrial DNA in ovarian tumors. METHODS: The D-loop region of 25 epithelial ovarian tumors together with the adjacent normal tissues were amplified by PCR and sequenced. RESULTS: Among the 25 ovarian tumors, 26 mutations were identified with the mutation rate of 32%. 19 mutations were detected in two cases of borderline carcinoma which was a special type of epithelial ovarian carcinoma. There were 6 microsatellite instabilities among the mutations and 11 new polymorphisms which were not reported previously in the GenBank. CONCLUSIONS: The D-loop region of mitochondrial DNA is a highly polymorphoric and mutable region and the mutation rate is relatively high in patients with ovarian tumors.
Assuntos
DNA Mitocondrial/genética , Neoplasias Ovarianas/genética , Mutação Puntual , Cistadenocarcinoma Seroso/genética , Feminino , Humanos , Polimorfismo GenéticoRESUMO
Topological insulators are classified according to their symmetries. Discovery of them in electronic solids is thus restricted by orbital and crystalline symmetries available in nature. Synthetic quantum matter, such as the recent double-well optical lattices loaded with s and p orbital ultracold atoms, can exploit symmetries and interaction beyond natural conditions. Here we unveil a topological phase of interacting fermionic atoms on a two-leg ladder derived from the above experimental optical lattice by dimension reduction. The topological band structure originates from the staggered phases of sp orbital tunnelling, requiring neither spin-orbit coupling nor other known mechanisms like p-wave pairing, artificial gauge field or rotation. Upon crossing over to two-dimensional coupled ladders, the edge modes from individual ladder form a parity-protected flat band at zero energy. Experimental signatures are found in density correlations and phase transitions to trivial band and Mott insulators.