Ethnomedicinal Plants and Herbal Preparations Used by Rural Communities in Tehsil Hajira (Poonch District of Azad Kashmir, Pakistan).

The present study emphasizes the importance of documenting ethnomedicinal plants and herbal practices of the local rural communities of Tehsil Hajira (Pakistan). The aim was to document, explore and quantify the traditional ethnomedicinal knowledge. Ethnobotanical data were collected using semi-structured questionnaires and analyzed using various quantitative indices. The results showed that 144 medicinal plant species from 70 families and 128 genera play an important role in herbal preparations. The most common type of preparation was powder (19.0%), followed by paste (16.7%), aqueous extract (15.7%), decoction (14.7%) and juice (11.0%). Fragaria nubicola (0.94) and Viola canescens (0.93) had the highest relative frequency of mention (RFC), while Berberis lycium (1.22) and Fragaria nubicola (1.18) had the highest use value (UV). Geranium wallichianum (85.5), Ligustrum lucidum (83) and Indigofera heterantha (71.5) were the most important species in the study area with the highest relative importance (RI) value. The diseases treated were categorized into 17 classes, with diseases of the digestive system and liver having the highest Informant Consensus Factor (ICF) value, followed by diseases of the oropharynx and musculoskeletal system. Important plants mentioned for the treatment of various diseases of the gastrointestinal tract are Zanthoxylum alatum, Berberis lycium, Mentha longifolia, Punica granatum, Rubus ellipticus and Viola canescens. New applications of rarely documented plants from this area are: Oxalis corniculata paste of the whole plant to treat vitiligo, Carthamus tinctorius flowers to treat chicken pox, Dioscorea deltoidea tuber powder to treat productive cough, Inula cappa root decoction to treat miscarriage, Habenaria digitata tuber juice for the treatment of fever, Viola canescens leaves and flowers for the treatment of sore throat and Achillea millefolium root and leaf juice for the treatment of pneumonia. These plants may contain interesting biochemical compounds and should be subjected to further pharmacological studies to develop new drugs. Traditional medicinal knowledge in the area under study is mainly limited to the elderly, traditional healers and midwives. Therefore, resource conservation strategies and future pharmacological studies are strongly recommended.

Folk Knowledge and Perceptions about the Use of Wild Fruits and Vegetables-Cross-Cultural Knowledge in the Pipli Pahar Reserved Forest of Okara, Pakistan.

Wild fruits and vegetables (WFVs) have been vital to local communities for centuries and make an important contribution to daily life and income. However, traditional knowledge of the use of wild fruits is at risk of being lost due to inadequate documentation. This study aimed to secure this knowledge through intermittent field visits and a semi-structured questionnaire. Using various ethnobotanical data analysis tools and SPSS (IBM 25), this study identified 65 WFV species (52 genera and 29 families). These species, mostly consumed as vegetables (49%) or fruits (43%), were predominantly herbaceous (48%) in wild and semi-wild habitats (67%). 20 WFVs were known to local communities (highest RFC), Phoenix sylvestris stood out as the most utilized species (highest UV). Surprisingly, only 23% of the WFVs were sold at markets. The survey identified 21 unique WFVs that are rarely documented for human consumption in Pakistan (e.g., Ehretia obtusifolia, Euploca strigosa, Brassica juncea, Cleome brachycarpa, Gymnosporia royleana, Cucumis maderaspatanus, Croton bonplandianus, Euphorbia prostrata, Vachellia nilotica, Pongamia pinnata, Grewia asiatica, Malvastrum coromandelianum, Morus serrata, Argemone mexicana, Bambusa vulgaris, Echinochloa colonum, Solanum virginianum, Physalis angulata, Withania somnifera, Zygophyllum creticum, and Peganum harmala), as well as 14 novel uses and five novel edible parts. Despite their ecological importance, the use of WFVs has declined because local people are unaware of their cultural and economic value. Preservation of traditional knowledge through education on conservation and utilization could boost economies and livelihoods in this and similar areas worldwide.

Development and application of a second-generation multilingual tool for invasion risk screening of non-native terrestrial plants.

Under the increasing threat to native ecosystems posed by non-native species invasions, there is an urgent need for decision support tools that can more effectively identify non-native species likely to become invasive. As part of the screening (first step) component in non-native species risk analysis, decision support tools have been developed for aquatic and terrestrial organisms. Amongst these tools is the Weed Risk Assessment (WRA) for screening non-native plants. The WRA has provided the foundations for developing the first-generation WRA-type Invasiveness Screening Kit (ISK) tools applicable to a range of aquatic species, and more recently for the second-generation ISK tools applicable to all aquatic organisms (including plants) and terrestrial animals. Given the most extensive usage of the latter toolkits, this study describes the development and application of the Terrestrial Plant Species Invasiveness Screening Kit (TPS-ISK). As a second-generation ISK tool, the TPS-ISK is a multilingual turnkey application that provides several advantages relative to the WRA: (i) compliance with the minimum standards against which a protocol should be evaluated for invasion process and management approaches; (ii) enhanced questionnaire comprehensiveness including a climate change component; (iii) provision of a level of confidence; (iv) error-free computation of risk scores; (v) multilingual support; (vi) possibility for across-study comparisons of screening outcomes; (vii) a powerful graphical user interface; (viii) seamless software deployment and accessibility with improved data exchange. The TPS-ISK successfully risk-ranked five representative sample species for the main taxonomic groups supported by the tool and ten angiosperms previously screened with the WRA for Turkey. The almost 20-year continuous development and evolution of the ISK tools, as opposed to the WRA, closely meet the increasing demand by scientists and decision-makers for a reliable, comprehensive, updatable and easily deployable decision support tool. For terrestrial plant screening, these requirements are therefore met by the newly developed TPS-ISK.

Ethnobotanical Insights into Medicinal and Culinary Plant Use: The Dwindling Traditional Heritage of the Dard Ethnic Group in the Gurez Region of the Kashmir Valley, India.

This ethnobiological study addresses the complicated relationship between the Dard ethnic group and their natural environment in the Gurez region of the Kashmir Valley. The study documents their traditional knowledge of the use of plant species for medicinal and culinary purposes. A total of 87 plant species from 41 different families were cataloged, with the Asteraceae family (15 species) and the Lamiaceae family (12 species) being the most commonly used. These plants were found to be used to treat 20 different ailments, with menstrual cramps being the most common (12 species). The fidelity values for these plants ranged from 11.10 to 71.42, demonstrating their importance in traditional medicine. In addition, 17 plant species were found to be useful for gastronomic purposes, with Juglans regia being the most valuable (use value of 0.73). The study also evaluated the conservation status of these plants and found that seven of them are considered critically endangered, ten endangered, and four endangered according to the IUCN classification. This study offers insights into the Dard people's deep connection to their natural environment and has significant implications for policy formulation, cultural conservation, and sustainable use of endemic species, as well as potential applications in pharmaceutical research for therapeutic compounds.

Traditional Ethnobotanical Knowledge of the Central Lika Region (Continental Croatia)-First Record of Edible Use of Fungus Taphrina pruni.

This study analyzed the use of plants and fungi, some wild and some cultivated, in three municipalities of Lika-Senj County (Perusic, Gospic and Lovinac). The range of the study area was about 60 km. Forty in-depth semi-structured interviews were performed. The use of 111 plant taxa from 50 plant families and five taxa of mushrooms and fungi belonging to five families was recorded (on average 27 taxa per interview). The results showed quite large differences between the three studied areas in terms of ethnobotanical and ecological knowledge. In the Perusic area, (101 taxa mentioned), some people still use wild plants on a daily basis for various purposes. The most commonly noted plants are Prunus spinosa, Taraxacum spp., Rosa canina, Urtica dioica, Juglans regia and Fragaria vesca. In the Lovinac region, people used fewer species of plants (76 species mentioned). The most common species used there are: Rosa canina, Achillea millefolium, Cornus mas, Crataegus monogyna, Sambucus nigra and Prunus domestica. In the town of Gospic, the collection and use of plants was not so widespread, with only 61 species mentioned, the most common being: Achillea millefolium, Cornus mas, Sambucus nigra, Viola sp., Prunus domestica and Rosa canina. The medicinal use of herbal tea Rubus caesius and Cydonia oblonga against diarrhea was well known in the study area and is used medicinally, mainly in the rural parts of the Gospic area. The consumption of the Sorbus species (S. aria, S. domestica and S. torminalis) is an interesting local tradition in Perusic and Lovinac. Species that are difficult to find in nature today and are no longer used include: Veratrum sp., Rhamnus alpinum ssp. fallax, Gentiana lutea and Ribes uva-crispa. The use of Chenopodium album has also died out. We can assume that the differences in ethnobotanical knowledge between the three studied areas are partly due to minor differences in climate and topography, while other causes lie in the higher degree of rurality and stronger ties to nature in the Lovinac and Perusic areas. The most important finding of the study is the use of the parasitic fungus Taphrina pruni (Fuckel) Tul. as a snack. The use of Helleborus dumetorum for ethnoveterinary practices is also worth noting. The traditional use of plants in the study area shows many signs of abandonment, and therefore efforts must be made to maintain the knowledge recorded in our study.

Divergence of Ethnobotanical Knowledge of Slovenians on the Edge of the Mediterranean as a Result of Historical, Geographical and Cultural Drivers.

State boundaries limit human contacts in a homogenous context of a landscape and its natural features, including plants. After nine centuries of separation, finally the two territories in Slovenia share the same political history. In this paper we tried to answer the question to which extent the past political borders, geographical and cultural drivers affect today's traditional knowledge on wild plants use of Slovenians, living unified in the same political entity. Data were collected using 60 in-depth semi-structured interviews, from March to August 2019, in two municipalities: Komen at Karst and Izola in Istria concerning food, medicinal, economic use, and local customs. The results indicate a quite large divergence in ethnobotanical and ecological knowledge between the two studied areas. In the Komen area, many people still use wild plants daily for various purposes (Taraxacum officinale, Melissa officinalis, Urtica dioica, Cornus mas, and Sambucus nigra). In contrast, this is limited to fewer people in the Izola area and mainly to seasonal use of specific plants (Asparagus acutifolius, Rosa canina, Salvia officinalis, Foeniculum vulgare and Rubus caesius). Unusual for the Mediterranean is the use of young shoots of Clematis vitalba, in the Izola area prepared as omelettes. We can assume that these differences are partly due to minor differences in climatic conditions and partly due to the influence of different cultures and cuisines. In the first place, the impact of Austro-Hungarian eating habits and cuisine can be seen on the area around Komen. Moreover, temporal "layers of knowledge" across the time scale are additionally mixed by the immigration of people from other parts of Slovenia or abroad, or with the influence of local herbal specialists. At last, we conclude Komen area knowledge is alive and homogeneous, and more connected to their local identity.

Insular Pharmacopoeias: Ethnobotanical Characteristics of Medicinal Plants Used on the Adriatic Islands.

The Adriatic islands in Croatia, usually divided into two archipelagos - the Kvarner and Dalmatian islands - is one of the largest groups of islands in Europe. Over 40 islands are still inhabited. Unfortunately the traditional use of medicinal plants was never properly documented there. Our data comes from 343 interviews carried out in 36 islands, including the 15 largest islands of the archipelago. The medicinal plants are mainly used to make herbal infusions or decoctions, occasionally medicinal liqueurs, syrups, compresses, or juices squeezed out of raw plants. We recorded the use of 146 taxa, among them 131 with at least one medicinal purpose and 15 only for tea. The frequency curve of use is relatively steep - several plants are used very frequently and most are reported only by one or two informants, which can be explained both by the large geographical spread of the area, and even more so by the devolution of local knowledge and disappearance of gathering practices due to specialization in tourism, modernization and depopulation. Most of the gathered plants already occur in ancient and medieval herbals and are a part of the pan-Mediterranean pharmacopoeia.

Plants in alcoholic beverages on the Croatian islands, with special reference to rakija travarica.

BACKGROUND: This paper aims to record the species used for flavouring and making alcoholic drinks, mainly rakija, on the islands of the Adriatic (Croatia). METHODS: Our data comes from 295 interviews performed on 36 islands, in both the Dalmatian and Kvarner areas of the Adriatic. RESULTS: Altogether, 114 species are used-46% from wild locations only, 15% both wild and cultivated, 38% only cultivated and two species are imported. The most common local alcohol is wine, made without spices, but grape pomace distillate is often flavoured with single or mixed species. The mix is called travarica. The most commonly used species are Foeniculum vulgare Mill., Myrtus communis L., Salvia officinalis L., Ruta graveolens L., Juniperus oxycedrus L., Ceratonia siliqua L., Juglans regia L., Citrus spp., Ficus carica L., Laurus nobilis L., Rosmarinus officinalis L., Artemisia absinthium L., Rosa centifolia L., Mentha × piperita L. and M. spicata L. Unfortunately, the widespread phenomenon of distilling Arbutus unedo L. fruits and fermenting Juniperus 'wine' is now extinct. Apart from grapes, the only commonly distilled fruit now is Ficus carica. CONCLUSIONS: It is striking that nearly all the plants are either wild or cultivated locally, which, in addition to the fact that the alcohol is made locally, shows the incredible local culinary self-sufficiency of the area. The number of species used is also very impressive.

The ethnobotany and biogeography of wild vegetables in the Adriatic islands.

BACKGROUND: Archipelagos of islands have played an important role in shaping some of the paradigms of biology, including the theory of the evolution of species. Later, their importance in biology was further emphasised by the theory of island biogeography, which contributed to a better understanding of the shaping of species richness not only on real islands, but on isolated habitat islands as well. Although ethnobotany is a well-established discipline, patterns of knowledge about plant uses in archipelagos have never been quantitatively analysed, and the whole concept has been only briefly mentioned in the ethnobiological context. The aim of our study was to record which taxa of wild vegetables have been consumed in the Adriatic islands and to establish if such variables as island size, population size, flora or its isolation are correlated with the number of wild vegetables used. METHODS: We interviewed 225 people (15 from each island). RESULTS: Altogether, the use of 89 species of wild vegetables has been recorded. The largest number of wild vegetables is eaten on the islands of Korcula, Vis and Solta, and the lowest on Ugljan, Cres and Dugi Otok. The studied independent variables had a small and statistically not significant effect on the wild vegetable list length. The most visible effect was an increasing trend from north-west to south-east, overrunning the typical biogeographical island patterns. Moreover, one of the large and well-populated islands, Korcula, showed an 'unusually' high level of wild vegetable use. We hypothesise that the current use of so many species on this island has been maintained by the inhabitants' awareness that they are the holders of relic knowledge, an awareness reiterated by ethnographic and popular publications, as well as a strong history of famine. The most interesting edible species used in the Adriatic islands are Bunium alpinum, Cytinus hypocystis (both mainly on Pasman), Lotus edulis (on Vis) and Posidonia oceanica (on Vis and Korcula). CONCLUSIONS: The recorded relationships between the demographic and geographical features of the islands were statistically not significant. We assume that cultural and historical factors diversifying the use of plants in particular islands are stronger than the above-mentioned measurable variables.

Using Ellenberg-Pignatti values to estimate habitat preferences of wild food and medicinal plants: an example from northeastern Istria (Croatia).

BACKGROUND: The paper presents the first ethnobotanical application of Ellenberg indicator values, which are widely used in European plant ecology. The aim of the study was to find out if Ellenberg values (indicating habitat preferences) differ for wild food and medicinal plants used in north-eastern Istria (Croatia). We used Ellenberg-Pignatti values (the version of Ellenberg values used in this part of Europe). METHODS: Fifty semi-structured interviews were carried out among local key informants, asking which wild food and medicinal plants they used. RESULTS: The mean number of food and medicinal plants mentioned per interview was 30. Altogether, 121 species were recorded as food or medicine used or previously used in the study area. Thirty-one species are used exclusively as food or everyday drink, 50 species are used exclusively as medicine and 40 species are used for both food and medicine. There were no significant differences between Ellenberg values for food and medicinal plants, apart from the Nitrogen indicator value - the plants used exclusively as food had a significantly higher index than those used in medicine. This probably stems from the fact that plants with soft fleshy shoots are attractive as food and they are more likely to come from nitrogen-rich ruderal habitats. CONCLUSIONS: Food plants and medicinal plants are collected from a variety of habitats and no clear difference between the two categories of plants was detected, however further testing of Ellenberg values in ethnobotanical studies could be interesting.