1.
Gene
; 32(1-2): 21-30, 1984 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-6099310
RESUMO
Streptomyces lividans 66 was transformed with a plasmid containing the regulatory region of the Streptomyces fradiae aph gene and a structural gene that specifies bovine growth hormone (bGH). When grown in liquid culture the transformant contained a protein identical to authentic bGH, as judged by radioimmunoassay and immuno-blotting (Western analysis). The bGH was present in cells that had been in culture for up to four weeks but was not found in the medium. The strategy employed should be generally applicable to the expression of foreign genes in actinomycetes.
Assuntos
Hormônio do Crescimento/biossíntese , Streptomyces/genética , Animais , Sequência de Bases , Bovinos , Clonagem Molecular , Genes , Vetores Genéticos , Hormônio do Crescimento/genética , Hormônio do Crescimento/imunologia , Plasmídeos
2.
J Am Podiatry Assoc
; 73(11): 589-92, 1983 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-6643911