Switchable terahertz orbital angular momentum Bessel beams based on spin-decoupled multifunctional reflective metasurfaces.

In this study, switchable terahertz (THz) multi-orbital angular momentum (OAM) Bessel beams (BBs) were developed based on a spin-decoupled reflective multifunctional metasurface (MTS). Switchability was achieved by switching the feed between left-hand circular polarization (LCP), right-hand circular polarization (RCP), and linear polarization (LP) incidences. A switchable physical model was established for calculating the beam direction, OAM mode, polarization, and non-diffractive distance of the outgoing BBs. As an example, a spin-decoupled MTS was designed to generate dual BBs under LCP incidence, which was subsequently switched to RCP or LP for switchability. The outgoing BBs could be switched among three types of beams: Type-1 under LCP incidence (LCP, θL = 40°, φL = 0°, lL = 1, dL = 18 cm) and (RCP, θR = -40°, φR = 0°, lR = -1, dR = 20 cm); Type-2 under RCP incidence (RCP, θR = 40°, φR = 0°, lR = 1, dR = 18 cm) and (LCP, θL = -19°, φL = 0°, lL = 3, dL = 16.4 cm); and Type-3 under LP incidence (LP, θ = 40°, φ = 0°, l = 1, d = 18 cm), (RCP, θR = -40°, φR = 0°, lR = -1, dR = 20 cm) and (LCP, θL = -19°, φL = 0°, lL = 3, dL = 16.4 cm). Compared with previous MTSs, the proposed spin-decoupled MTS has the advantages of switchability among BBs, high non-diffractive distance/aperture size ratio of 15, large beam deflection angle of up to 40°, and high BB conversion efficiency of up to 96%. The simulated results were consistent with those calculated using the physical model, thus validating the physical model. The designed switchable BBs have potential THz near-field applications, such as high-capacity near-field wireless communications, wireless power transfer, high-resolution imaging, non-destructive testing, and speed detection of high-speed rotating objects.

Diversity and Biocontrol Potential of Endophytic Fungi and Bacteria Associated with Healthy Welsh Onion Leaves in Taiwan.

Foliar diseases caused by Stemphylium and Colletotrichum species are among the major biotic factors limiting Welsh onion production in Taiwan. Owing to concerns about the environment and the development of pathogen resistance to existing fungicides, biological control using endophytes is emerging as an eco-friendly alternative to chemical control. The aim of the present study was to isolate endophytes from healthy Welsh onion leaves and investigate their antagonistic potential against the major phytopathogenic fungi associated with Welsh onion plants in Taiwan. A total of 109 bacterial and 31 fungal strains were isolated from healthy Welsh onion leaves and assigned to 16 bacterial and nine fungal genera using morphological and molecular characterization based on DNA sequence data obtained from nuclear internal transcribed spacer (nrITS) (fungi) and 16S rRNA (bacteria). Evaluation of these endophytic isolates for biocontrol activity against leaf blight pathogens Colletotrichum spaethianum strain SX15-2 and Stemphylium vesicarium strain SX20-2 by dual culture assay and greenhouse experiments resulted in the identification of two bacterial isolates (GFB08 and LFB28) and two fungal isolates (GFF06 and GFF08) as promising antagonists to leaf blight pathogens. Among the four selected isolates, Bacillus strain GFB08 exhibited the highest disease control in the greenhouse study. Therefore, Bacillus strain GFB08 was further evaluated to understand the mechanism underlying its biocontrol efficacy. A phylogenetic analysis based on six genes identified Bacillus strain GFB08 as B. velezensis. The presence of antimicrobial peptide genes (baer, bamC, bmyB, dfnA, fenD, ituC, mlna, and srfAA) and the secretion of several cell wall degrading enzymes (CWDEs), including cellulase and protease, confirmed the antifungal nature of B. velezensis strain GFB08. Leaf blight disease suppression by preventive and curative assays indicated that B. velezensis strain GFB08 has preventive efficacy on C. spaethianum strain SX15-2 and both preventive and curative efficacy on S. vesicarium strain SX20-2. Overall, the current study revealed that healthy Welsh onion leaves harbour diverse bacterial and fungal endophytes, among which the endophytic bacterial strain, B. velezensis strain GFB08, could potentially be used as a biocontrol agent to manage the leaf blight diseases of Welsh onion in Taiwan.

Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan.

Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.

Prevention effect of medical self-crosslinking sodium hyaluronate gel on epidural scar adhesion after laminectomy.

OBJECTIVE: To analyze the effect and medical self-crosslinking sodium hyaluronate gel on epidural scar adhesion after laminectomy. METHODS: A total of 24 New Zealand L5 laminectomy rabbits were randomly divided into four groups, group A as the control group without any treatment; group B covered by sodium hyaluronate gel; group C and group D covered by 0.5 and 1.0 mL medical self-crosslinking sodium hyaluronate gel. All rabbits were scored at various time points after 2, 4, 6, 8, 12 weeks, then the formation of scar was observed. RESULTS: In Groups B, C, D loose scar tissue occurred after 2 weeks of the operation, scar tissues were significantly less than that in group A, with mild inflammatory reaction. After 8 weeks, the scar tissues of group B were significantly more than that of groups C, D. After two weeks, group B, C were back to the preoperative level; After 4 weeks, group D was back to the preoperative level; After four weeks, the CSEP of group A was increased significantly, which was significantly higher than that in groups B, C, D. The motor function score of group A, group B and group C were the same as preoperative, but that in group D it was decreased significantly, and then it gradually recovered. After 4 weeks it kept a stable level. The motor function score of group A was decreased gradually after the operation, which kept a stable level after 4 weeks, the motor function score was significantly lower than that in groups B, C, D. CONCLUSIONS: Determination of somatosensory evoked potentials is sensitive indicator of spinal cord injury; Application of medical self-crosslinking sodium hyaluronate gel is effective on epidural scar adhesion after laminectomy.