Application value of iodine-131 combined with levothyroxine sodium in patients with differentiated thyroid cancer after surgery.

INTRODUCTION: This study aimed to evaluate the clinical value of iodine-131 combined with levothyroxine sodium in the treatment of patients with differentiated thyroid cancer (DTC) after surgery. METHODS: Prospective randomized controlled studies were conducted. A total of 374 DTC patients who underwent total or near-total thyroidectomy in the Department of Thyroid Surgery, Tianjin Union Medical Center and Tianjin Medical University General Hospital from January 2019 to February 2022 were selected and divided into control group (187 cases) and observation group (187 cases) according to random number table method. The control group was treated with levothyroxine sodium after surgery, and the observation group was treated with iodine-131 on the basis of the control group. Gender, age, course of disease, tumor diameter, pathological type, TNM classification, treatment effect, Thyroglobulin (Tg) levels before and after treatment, SF-36 health status questionnaires (SF-36), occurrence of adverse reactions after treatment and recurrence rate of one year follow-up were compared and analyzed between the two groups. RESULTS: There was no significant difference in baseline data between the two groups. After treatment, the effective rate of the observation group increased by 11.23% compared to the control group, with a statistically significant difference (91.98% vs. 80.75%, P<0.05). There was no significant difference in Tg level and scores of SF-36 evaluation including physical functioning, physical problems, vitality, pain, mental health, emotional problems, social functioning, and general health perception between the two groups before surgery (P>0.05), Tg levels and scores of SF-36 evaluation in all dimensions were significantly improved in both groups after treatment (P<0.05), and the levels of Tg and scores of SF-36 in all dimensions in observation group were significantly better than those in control group after treatment (P<0.001). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). The recurrence rate in the observation group was 5.89% lower than that in the control group one year after treatment, with a statistically significant difference (2.67% vs. 8.56%, P<0.05). CONCLUSIONS: The combination of iodine-131 and levothyroxine sodium in the postoperative treatment of differentiated thyroid cancer can improve the therapeutic effect and reduce the postoperative recurrence rate without increasing adverse reactions, which is worthy of clinical reference and promotion.

Rational Construction of Co4(µ-O)6(COO)6 SBU-Based MOFs through Mixed-Ligand Strategy to Enhance Electrocatalytic Oxygen Evolution Performance.

Metal-organic frameworks (MOFs) are increasingly becoming an important choice for developing robust and efficient electrocatalysts; therefore, exploring the relationship between the structure, catalytic activity, and stability of MOFs is of great significance. MOFs 1-3 with different spatial configurations are designed and synthesized based on linear pyridine ligands, tetragonal carboxylic acid ligands, and triangular carboxylic acid ligands, while MOF 4 displays a three-dimensional (3D) supramolecule assembled through a mixed-ligand strategy. Compared with MOFs 1-3, MOF 4 has the lowest overpotential of 106 mV (at 10 mA·cm-2) and a Tafel slope of 80.9 mV·dec-1, as well as sturdy long-term stability in the process of oxygen evolution reaction (OER). The presence of dense metal clusters and µ3-O promotes the optimal catalytic performance of MOF 4. Density functional theory (DFT) calculations of MOF 4 demonstrate that the process from O* to OOH* is the rate-determining step. This investigation further reveals the relationship between MOF structural composition and electrocatalytic OER performance and provides an effective strategy for the assembly of MOF-based electrocatalysts.

Robust High-performance Bifunctional Porous Cobalt MOF-Based Catalysts for Overall Water Splitting.

MOF-based materials, as bifunctional catalysts for electrocatalytic water splitting, play an important role in the application and development of clean fuel hydrogen energy. This study presents a series of novel 3D Co-based MOFs with layered networks, including [Co(4,4'-bipy)0.5(aip)(CH3OH)·H2O]n (Co-MOF 1), [Co2(1,3'-bit)(aip)2(CH3OH)·H2O]n (Co-MOF 2), [Co(4,4'-bipb)(aip)]n (Co-MOF 3), and [Co2(4,4'-bipe)(aip)2·1.5H2O]n (Co-MOF 4). Their single-crystal structures of Co-MOFs 1-4 are characterized and analyzed before being applied in alkaline solutions for water decomposition (OER and HER). The electrocatalytic tests indicate that Co-MOFs 1-4 exhibit a good performance. Notably, Co-MOF 4 exhibits great behavior which has low overpotentials of 94 and 188 mV (OER) as well as 185 and 352 mV (HER) at the currents of 10 and 100 mA cm-2, respectively. In comparison with Co-MOFs 1-3, Co-MOF 4 has the lowest Tafel slopes, highest ECSA, and smallest resistance. The immanent qualities, such as distinct interwoven long chain layered structure, unsaturated coordination modes, and synergistic catalytic qualities among Co ions, contribute to explaining the results. The fundamentals provide valuable information for the investigation of innovative MOF-based bifunctional electrocatalysts for overall water splitting.

Pillar-Layered Porous Metal-Organic Frameworks with Co2N2O8 Clusters and Tetragonal Ligands for CO2 Conversion.

Converting CO2 to valuable chemicals and fuels is a viable method to establish a carbon-neutral energy cycle in the environment. Metal-organic frameworks (MOFs), characterized by dispersed active sites, high porosity, etc., have displayed a great application prospect in the electrochemical/chemical CO2 reduction reaction (CO2RR) process. Herein, we proposed a one-step production to establish a series of pillar-layered porous MOFs, [Co2(L)(bimb)]n (MOF 1) and [Co4(L)2(bidpe)2]n (MOF 2) [H4L = 5'-(4-carboxyphenyl)-(1,1':2',1″-terphenyl)-4,4',4″-tricarboxylic, bimb = 1,4-bis(imidazol-1-yl)-butane, bidpe = 4'-bis(imidazolyl) diphenyl ether], for preferential conversion of CO2 via ligand adjustment and increase of active sites' density. According to single-crystal X-ray diffraction studies, [Co2(L)(bimb)]n exhibits pillar-layered binuclear 3D frameworks with a 2,4,6-linked 3-nodes new topology structure, while [Co4(L)2(bidpe)2]n displays pillar-layered tetranuclear interspersed networks with a 4,6-linked 2-nodes fsc topology structure through a ligand adjustment strategy. Meanwhile, the pillar-layered structure of the MOFs with abundant active sites is conducive to mass diffusion and benefits the conversion of CO2. MOFs 1-2 exhibit good electrocatalytic activity for CO2RR in 0.5 M KHCO3 solution. Especially, the current density of MOF 2 generated at -0.90 V (vs. RHE) reaches -81.6 mA·cm-2, which is 3.1 times higher than that under an Ar atmosphere. In addition, MOFs 1-2 can be used as a heterogeneous catalyst for chemical conversion of CO2. The results are expected to provide inspiration for rational design to develop stable and high-efficiency MOF-based electrocatalysts for CO2RR.

High resistance risk of Phytophthora colocasiae to azoxystrobin in southeastern of China.

Quinone outside inhibitor (QoI) has been applied to manage taro leaf blight caused by Phytophthora colocasiae in southeastern of China for many years. The risk of P. colocasiae to QoI and the potential resistant mechanism remain unknown. In this study, the 74 P. colocasiae strains were sampled from southeastern of China. Sequence analysis of the QoI target Cytb showed one nucleotide variant in the fragment of this gene in this population, producing two haplotypes. The nucleotide variant leads to codon change at 142 (GGT to GCT) producing A142 (alanine) and G142 (glycine) in Hap_1 and Hap_2 strains, respectively. The sensitivity differentiation to azoxystrobin of two haplotypes were observed in vitro. The Hap_1 and Hap_2 strains were confirmed resistant and sensitive by control efficacy of label rate fungicide application, which was 3.0% and 88.8% treated with 500 µg/mL azoxystrobin, respectively. In addition, 10.0 µg/mL azoxystrobin plus 50 µg/mL salicylhydroxamic acid (SHAM) supplemented in PDA medium was identified as a discriminatory dose for differentiation of these two phenotype strains. The azoxystrobin resistant frequency reached 86.5%, indicating prevalence of QoI resistance in the field. Further fitness related features showed that no significant difference in temperature sensitivity, mycelial growth rate, sporangia production, zoospore release and aggressiveness between azoxystrobin-resistant and sensitive strains indicating no potential fitness cost for azoxystrobin resistance. Taken together, azoxystrobin resistance need to be taken into consideration to manage taro leaf blight in southeastern of China.

Designed Construction of 2D Honeycomb Cationic MOF Materials for Selective Removal of Sulfonic Anionic Dyes.

Various carcinogenic dyes in water bodies are difficult to degrade due to their stability to light and oxidants, causing extended pollution. In this study, MOF 1 ({[Co(tib)2]·(H2O)2·SO4}n) and MOF 2 ({[Cu(tib)2]·(H2O)2·SO4}n) (tib = 1,3,5-tirs(1-imidazolyl)benzene) were synthesized by the solvothermal method. MOFs 1 and 2 were successfully characterized by single-crystal X-ray diffraction (XRD) and powder X-ray diffraction (PXRD). Based on the structural characteristics of MOFs 1 and 2, we designed two cationic MOF material skeletons, namely, MOFs I and II ([Co(tib)22+]n and [Cu(tib)22+]n), which were obtained by calcination in combination with the thermogravimetric curve to remove the free components in the lattice. As expected, MOFs I and II showed an excellent adsorption effect on sulfonic anionic dyes. Notably, the adsorption capacity of MOF I can reach 2922.8 mg g-1 for Congo Red (CR) at room temperature (RT). The adsorption process fits the pseudo-second-order kinetic model and Freundlich isotherm model. Moreover, zeta potential tests and quantum chemical calculations indicate that electrostatic interactions and hydrogen bond between the hydroxyl group on the sulfonic acid group and the N atom on the imidazole ring mainly promote the adsorption of CR dyes on MOF I. MOFs I and II are revealed as a promising novel adsorption material to remove hazardous organic aromatic pollutants with high efficiency in future endeavors.

Three-Dimensional Cadmium-Organic Framework with Dual Functions of Oxygen Evolution in Water Splitting and Fenton-like Photocatalytic Removal of Organic Pollutants.

Metal-organic frameworks (MOFs) have exhibited appreciable potential as catalytic agents in the field of material science. The research of new MOFs with dual functions in electrocatalysis and photocatalysis under ultraviolet (UV) irradiation is extremely pivotal for renewable energy applications. Hence, we synthesized a series of three-dimensional MOFs, namely, [Cd(bimb)2(HITA)2]n (Cd-MOF 1), {[Cd(bimb)6](NO3)2}n (Cd-MOF 2), and [Cd(bimb)4(ONO2)2]n (Cd-MOF 3) (bimb = 1,4-bis(imidazol-1-ylmethyl)benzene; H2ITA = 5-hydroxyisophthalic acid), with applicability in the oxygen evolution reaction process and Fenton-like photocatalysis. The obtained results show that Cd-MOF 1 exhibited the most remarkable catalytic performance, affording a current density of 10 mA cm-2 at a very low overpotential of 279 mV and the smallest Tafel slope of 85.13 mV dec-1. Meanwhile, these MOFs can generate hydroxyl radicals (•OH) under UV light irradiation with the existence of H2O2, enabling the rapid degradation of organic pollutants. This study provides a valuable direction for producing multifunctional and environmentally friendly catalysts.

In Vivo Tracking of Persistent Organic Pollutants via a Coaxially Integrated and Implanted Photofuel Microsensor.

In vivo tracking of persistent organic pollutants (POPs) is of great significance for assessing their risks to the ecological environment and human health. However, existing in vivo POPs detection methods are limited by the lethal sampling of living organisms, complex sample preparation processes, or bulky testing equipment. Photoelectrochemical (PEC) sensing with the merits of high sensitivity and simple equipment is a fast-developed method for in vivo analysis. A major obstacle for in vivo PEC sensors is the separated implantation of multiple electrodes and a light source, which raises concerns like multielectrode biofouling and electroactive molecules interference in the complex environment, uncertain electrode implant distance, and multiple insertion operations. Here, a coaxially implanted photofuel microsensor was developed by hiding the optical fiber-based photoanode inside the glass capillary-based biocathode, and the model target PCB77 can be detected with an ultralow detection limit (2.8 fg/mL). This unique photoanode-biocathode-light source integrated structure ensures excellent selectivity, good antifouling ability and biocompatibility, high accuracy, and less implant mechanical damage. Combined with a handheld pH meter, our sensor achieved convenient and direct tracking of the bioaccumulation levels of PCB77 in freely swimming fish.

Electrocatalytic CO2 Reduction and H2 Evolution by a Copper (II) Complex with Redox-Active Ligand.

The process of electrocatalytic CO2 reduction and H2 evolution from water, regarding renewable energy, has become one of the global solutions to problems related to energy consumption and environmental degradation. In order to promote the electrocatalytic reactivity, the study of the role of ligands in catalysis has attracted more and more attention. Herein, we have developed a copper (II) complex with redox-active ligand [Cu(L1)2NO3]NO3 (1, L1 = 2-(6-methoxypyridin-2-yl)-6-nitro-1h-benzo [D] imidazole). X-ray crystallography reveals that the Cu ion in cation of complex 1 is coordinated by two redox ligands L1 and one labile nitrate ligand, which could assist the metal center for catalysis. The longer Cu-O bond between the metal center and the labile nitrate ligand would break to provide an open coordination site for the binding of the substrate during the catalytic process. The electrocatalytic investigation combined with DFT calculations demonstrate that the copper (II) complex could homogeneously catalyze CO2 reduction towards CO and H2 evolution, and this could occur with great performance due to the cooperative effect between the central Cu (II) ion and the redox- active ligand L1. Further, we discovered that the added proton source H2O and TsOH·H2O (p-Toluenesulfonic acid) could greatly enhance its electrocatalytic activity for CO2 reduction and H2 evolution, respectively.

Two biologically inspired tetranuclear nickel(II) catalysts: effect of the geometry of Ni4 core on electrocatalytic water oxidation.

Two biologically inspired tetranuclear nickel complexes [Ni4(L-H)4(CH3COO)3]·Cl (1) and [Ni4(L-H)4(CH3COO)4]·2CH3OH (2) (L = di(pyridin-2-yl)methanediol) have been synthesized and investigated by a combination of X-ray crystallography, PXRD, electrochemistry, in-situ UV-Vis spectroelectrochemistry and DLS. Both of the two complexes feature a core composed of four Ni(II) ions with the same peripheral ligation provided by the anionic di(pyridin-2-yl)methanediol and MeCOO- ligands. Whereas, complex 1 possesses one distorted cubane-like [Ni4(µ3-O)4] core, while 2 has one extended butterfly-like [Ni4(µ3-O)2] core. The homogeneous electrocatalytic reactivity of the two water-soluble complexes for water oxidation have been thoroughly studied, which demonstrates that both of them can efficiently electrocatalyze water oxidation with high stability under alkaline conditions, at relatively low over-potentials (η) of 420-790 mV for 1 and 390-780 mV for 2, both in the pH range of 7.67-12.32, with the high TOF of about 139 s-1 (1) and 69 s-1 (2) at pH = 12.32, respectively. By a series of comparative experiments for complexes 1 and 2, we proposed that their crystal geometries play an important role in their electrocatalytic reactivity for water oxidation. We verified that biomimetic cubane geometry could promote OER catalysis with two very similar compounds for the first time. Compared with 2, the biomimetic cubane topology of 1 could promote OER catalysis by facilitating efficient charge delocalization and electron-transfer.

[Quality standards of Gleditsiae Spina and its decoction pieces].

In view of the current inadequate standards for Gleditsiae Spina in the Chinese Pharmacopoeia, this study put forward some new items of the quality standards of Gleditsiae Spina. Thin-layer chromatography(TLC) was performed for identification with the reference substance of taxifolin and the reference material of Gleditsiae Spina as the control. According to the general principles of the Chinese Pharmacopoeia(2020 edition, Vol. 4), the moisture, total ash content, and alcohol-soluble extract of medicinal materials and decoction pieces of Gleditsiae Spina were determined. The content determination method for medicinal materials and decoction pieces of Gleditsiae Spina was established using high-performance liquid chromatography(HPLC), with taxifolin as the quality control index. Based on the determination results of 30 batches of samples of Gleditsiae Spina from different habitats, the draft quality standards of Gleditsiae Spina were developed, which provided suggestions for the revision of the quality standards of Gleditsiae Spina in the Chinese Pharmacopoeia.

Identification of differentially expressed circRNAs and a novel hsa_circ_0000144 that promote tumor growth in gastric cancer.

BACKGROUND: Circular RNAs (circRNAs) are involved in regulating tumor pathogenesis. The mechanism of circRNAs in gastric cancer (GC) is still unknown. Our study aimed to identify differentially expressed circRNAs and assess a novel circRNA (hsa_circ_0000144) in the proliferation, migration, and invasion in GC. METHODS: Gene ontology (GO) enrichment and analyses of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, pathway network, and the ceRNA regulatory network of hsa_circ_0000144 targeting miRNAs and mRNAs were performed with the help of bioinformatics using R language and Perl software. hsa_circ_0000144 expression and circRNA knockdown in GC cell lines were detected using quantitative PCR (qPCR) in vitro. Cell proliferation, migration, and invasion after circRNA knockdown were measured using the cell counting kit-8 assay and Transwell assay. RESULTS: The circRNA expression profile GSE78092 downloaded from the Gene Expression Omnibus database included three GC patients and three normal tissues. Thirty-two differentially expressed circRNAs comprised six upregulated circRNAs and 26 downregulated circRNAs. In particular, the ErbB signaling pathway, neurotrophin signaling pathway, cellular senescence, and pathways in bladder cancer and GC played the most important roles in the pathway network. The expression of hsa_circ_0000144 was upregulated in GC cell lines. Hsa_circ_0000144 knockdown suppressed tumor growth in vitro. CONCLUSIONS: Hsa_circ_0000144 promotes GC cell proliferation, migration, and invasion, and the ceRNA regulatory network of hsa_circ_0000144 targeting miRNAs and mRNAs might be biomarkers for GC diagnosis and targeted therapy.

Bibliometric analysis and review of mine ventilation literature published between 2010 and 2023.

To provide scholars with a quick understanding of the current status, research hotspots, and future trends in the field of mine ventilation, this paper conducted a visualized bibliometric analysis and a comprehensive review of mine ventilation-related literature from 2010 to 2023 using CiteSpace. A thorough analysis of the publication time, co-authorship, co-citation, keywords, and research topics of the literature was carried out. Based on this, through systematic literature reading and summarization, research topics in the field of mine ventilation were organized, analyzed, and classified. The results indicate that mine ventilation research from 2010 to 2023 went through three stages: stable development, slow growth, and rapid ascent. Nie Wen and China Univ Min & Technol were the most prolific authors and institutions in the field of mine ventilation. China had the highest number of publications during 2010-2023, while Canada and Poland exhibited the highest centrality, signifying their key roles in the mine ventilation domain. Deep mine ventilation and intelligent mine ventilation emerged as research hotspots and mainstream trends in the future. The analysis of multiple hazard coupling studies represents a research direction that mine ventilation needs to develop. Numerical simulation techniques should not be limited to static analysis, as dynamic simulation is a focal area of interest.

Swellable microneedle-coupled light-addressable photoelectrochemical sensor for in-situ tracking of multiple pesticides pollution in vivo.

In vivo monitoring of multiple pesticide contamination is of great significance for evaluating the health risks of different pesticides, agricultural production safety, and ecological and environmental assessment. Here, we report a hydrogel microneedle array coupled light-addressable photoelectrochemical sensor for tracking multiple pesticide uptake and elimination in living animals and plants, holding three prominent merits: i) enables in-situ detection of in vivo pesticides, avoiding cumbersome and complex sample transportation and handling processes; ii) allows repeated in vivo sampling of the same organism, improving tracking test controllability and accuracy; iii) avoids lethal sampling, providing a better understanding of the pesticides fate in living organisms. The coupled sensor is mechanically robust for withstanding more than 0.35 N per needle and highly swellable (800 %) for timely extraction of sufficient in vivo solution for analysis. For proof-of-concept, it achieves in-situ detection of atrazine, acetamiprid, and carbendazim efficiently and quantitatively in artificial agarose skin models, mouse skin interstitial fluids, and plant leaves with little inflammatory reaction. This simple, highly integrated, minimally invasive, and high-throughput in vivo monitoring method is ideal for future field environmental monitoring and plant and animal disease diagnosis.

Transcriptome Analysis of Intermittent Light Induced Early Bolting in Flowering Chinese Cabbage.

In flowering Chinese cabbage, early booting is one of the most important characteristics that is linked with quality and production. Through fixed light intensity (280 µmol·m-2·s-1) and fixed intermittent lighting in flowering Chinese cabbage, there was early bolting, bud emergence, and flowering. Moreover, the aboveground fresh weight, blade area, dry weight of blade, and quantification of the leaves in flowering Chinese cabbage were significantly reduced, while the thickness of tillers, tillers height, dry weight of tillers, and tillers weight were significantly increased. The chlorophyll contents and soil-plant analysis and development (SPAD) value decreased in the early stage and increased in the later stage. The nitrate content decreased, while the photosynthetic rate, vitamin C content, soluble sugar content, soluble protein content, phenolic content, and flavonoid content increased, and mineral elements also accumulated. In order to explore the mechanism of intermittent light promoting the early bolting and flowering of '49d' flowering Chinese cabbage, this study analyzed the transcriptional regulation from a global perspective using RNA sequencing. A total of 17,086 differentially expressed genes (DEGs) were obtained and 396 DEGs were selected that were closely related to early bolting. These DEGs were mainly involved in pollen wall assembly and plant circadian rhythm pathways, light action (34 DEGs), hormone biosynthesis and regulation (26 DEGs), development (21 DEGs), and carbohydrate synthesis and transport (6 DEGs). Three hub genes with the highest connectivity were identified through weighted gene co-expression network analysis (WGCNA): BrRVE, BrLHY, and BrRVE1. It is speculated that they may be involved in the intermittent light regulation of early bolting in flowering Chinese cabbage. In conclusion, intermittent light can be used as a useful tool to regulate plant growth structure, increase planting density, enhance photosynthesis, increase mineral accumulation, accelerate growth, and shorten the breeding cycle.

Zero-valent iron (nZVI) nanoparticles mediate SlERF1 expression to enhance cadmium stress tolerance in tomato.

Cadmium (Cd) pollution threatens plant physiological and biochemical activities and crop production. Significant progress has been made in characterizing how nanoparticles affect Cd stress tolerance; however, the molecular mechanism of nZVI nanoparticles in Cd stress remains largely uncharacterized. Plants treated with nZVI and exposed to Cd had increased antioxidant capacity and reduced Cd accumulation in plant tissues. The nZVI treatment differentially affected the expression of genes involved in plant environmental responses, including those associated with the ERF transcription factor. SlEFR1 was upregulated by Cd stress in nZVI-treated plants when compared with the control and the predicted protein-protein interactions suggested SlERF1 interacts with proteins associated with plant hormone signaling pathway and related to stress. Yeast overexpressing SlEFR1 grew faster after Cd exposure and significantly had higher Cd stress tolerance when compared with empty vector controls. These results suggest that nZVI induces Cd stress tolerance by activating SlERF1 expression to improve plant growth and nutrient accumulation. Our study reveals the molecular mechanism of Cd stress tolerance for improved plant growth and will support new research on overcoming Cd stress and improving vegetable crop production.

Diffusion Characteristics of Airflow and CO in the Dead-End Tunnel with Different Ventilation Parameters after Tunneling Blasting.

After tunnel blasting, a large amount of CO will be produced and accumulated in the dead-end tunnel. If the ventilation discharge is not proper and the entry time into the dead-end tunnel is not appropriate, then it can cause workers to suffer from poisoning, hypoxia, and suffocation. Therefore, to understand the airflow and diffusion characteristics of CO in the dead-end tunnel after excavation and improve the working environment quality of the heading excavation tunnel, this paper uses numerical simulation and on-site verification to study the influence of different ventilation parameters on the airflow and CO diffusion characteristics in the dead-end tunnel after excavation and blasting. The research results show that the higher the air velocity of the duct, the smaller the distance between the duct and the working face, and the higher the hanging height of the duct, the easier it is for CO to be discharged from the dead-end tunnel. The larger the distance between the duct and the side wall, the more vortices there are in the dead-end tunnel and the more difficult it is to discharge CO from the tunnel. This study provides theoretical guidance for the research of the migration law of CO after tunnel blasting and has important value for ensuring a safe working environment and clean production in tunnel excavation.

A novel autophagy-related long non-coding RNAs signature predicting progression-free interval and I-131 therapy benefits in papillary thyroid carcinoma.

This study aimed to explore the prognostic and predictive value of autophagy-related lncRNAs in papillary thyroid carcinoma (PTC). The expression data of autophagy-related genes and lncRNAs of the PTC patients were obtained from TCGA database. Autophagy-related-differentially expressed lncRNAs (DElncs) were identified and used to establish the lncRNAs signature predicting patients' progression-free interval (PFI) in the training cohort. Its performance was assessed in the training cohort, validation cohort, and entire cohort. Effects of the signature on I-131 therapy were also explored. We identified 199 autophagy-related-DElncs and constructed a novel six-lncRNAs signature was constructed based on these lncRNAs. This signature had a good predictive performance and was superior to TNM stages and previous clinical risk scores. I-131 therapy was found to be associated with favorable prognosis in patients with high-risk scores but not those with low-risk scores. Gene set enrichment analysis suggested that a series of hallmark gene sets were enriched in the high-risk subgroup. Single-cell RNA sequencing analysis suggested that the lncRNAs were mainly expressed in thyroid cells but not stromal cells. In conclusion, our study constructed a well-performed six-lncRNAs signature to predict PFI and I-131 therapy benefits in PTC.

ABCB11 accumulated in immature tertiary lymphoid structures participates in xenobiotic metabolic process and predicts resistance to PD-1/PD-L1 inhibitors in head and neck squamous cell carcinoma.

Head and neck squamous cell carcinomas (HNSCC) are at a high risk of recurrence and multimodal therapy have not significantly improved survival in recent decades. Although immune checkpoint inhibitors (ICIs) are effective in a small proportion of HNSCC patients, the majority do not respond. In this study, we for the first time revealed that xenobiotic metabolic process was significantly associated with resistance to programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) inhibitors in HNSCC and found that ATP binding cassette subfamily B member 11 (ABCB11) accumulated in immature tertiary lymphoid structures (TLSs) predicted worse progression-free survival (PFS) and overall survival (OS) after PD-1/PD-L1 inhibitors therapy. Moreover, the expression of cytochrome P450 1A2 (CYP1A2), a cytochrome P450 (CYP) enzyme that participates in xenobiotic metabolic process, was significantly upregulated in CD45+ABCB11+ tumor-infiltrating lymphocytes (TILs) compared with CD45+ABCB11-TILs in HNSCC tissues. Whole slide scans of 110 HNSCC tissues with hematoxylin-eosin (HE) and multispectral immuno-fluorescent (mIF) staining revealed that ABCB11 had a high co-expression with CYP1A2 in immature TLSs, and colocalization of ABCB11 and CYP1A2 in immature TLs significantly associated with high infiltration of immunosuppressive T-regulatory (Treg). Our study revealed that ABCB11 accumulated in immature TLSs might upregulate CYP1A2 to mediate xenobiotic metabolic process, thus increase the immunosuppressive Treg infiltration, and induce resistance to PD-1/PD-L1 inhibitors in HNSCC.

Changes in the Structures and Directions of Heavy Metal-Contaminated Soil Remediation Research from 1999 to 2020: A Bibliometric & Scientometric Study.

The pollution of heavy metals in soil is a problem of great concern to international scholars today. This research investigates the current research activities in the field of soil heavy metal pollution remediation and discusses the current areas of research focus and development trends. We conducted a bibliometric analysis of the literature on soil heavy metal pollution remediation from 1999 to 2020. CiteSpace and Vosviewer were used to conduct document co-citation and cluster analyses on the collected data. The research was mainly carried out based on the following factors: chronological distribution, country and institution distribution, source journal analysis, keyword co-occurrence analysis, and reference co-citation analysis. China (2173, 28.64%) and the United States (946, 12.47%) are the top two countries in terms of the number of articles published, and Environmental Science and Pollution Research (384, 5.06%) and Science of the Total Environment (345, 4.55%) published the most articles. The Chinese Academy of Science (485) is the organization that has contributed the most to the total number of publications. Furthermore, based on a keyword co-word analysis with Vosviewer and CitesSpace, it was concluded that the applications of phytoremediation and biochar in the remediation of heavy metals in soil are current research hotspots. Additionally, future research should focus on repair mechanisms, the development of new repair technology and joint repair systems.