RESUMO
A motile, Gram-stain-negative, non-pigmented bacterial strain, designated MGL06T, was isolated from seawater of the South China Sea on selection medium containing 0.1 % (w/v) malachite green. Strain MGL06T showed highest 16S rRNA gene sequence similarity to Rhizobium vignae CCBAU 05176T (97.2 %), and shared 93.2-96.9 % with the type strains of other recognized Rhizobium species. Phylogenetic analyses based on 16S rRNA and housekeeping gene sequences showed that strain MGL06T belonged to the genus Rhizobium. Mean levels of DNA-DNA relatedness between strain MGL06T and R. vignae CCBAU 05176T, Rhizobium huautlense S02T and Rhizobium alkalisoli CCBAU 01393T were 20 ± 3, 18 ± 2 and 14 ± 3 %, respectively, indicating that strain MGL06T was distinct from them genetically. Strain MGL06T did not form nodules on three different legumes, and the nodD and nifH genes were also not detected by PCR or based on the draft genome sequence. Strain MGL06T contained Q-10 as the predominant ubiquinone. The major fatty acid was C18 : 1ω7c/C18 : 1ω6c with minor amounts of C19 : 0 cyclo ω8c, C16 : 0 and C18 : 1ω7c 11-methyl. Polar lipids of strain MGL06T included unknown glycolipids, phosphatidylcholine, aminolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unknown polar lipid and aminophospholipid. Based on its phenotypic and genotypic data, strain MGL06T represents a novel species of the genus Rhizobium, for which the name Rhizobium marinum sp. nov. is proposed. The type strain is MGL06T ( = MCCC 1A00836T = JCM 30155T).
Assuntos
Filogenia , Rhizobium/classificação , Água do Mar/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Fabaceae/microbiologia , Ácidos Graxos/química , Genes Bacterianos , Glicolipídeos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Nodulação , RNA Ribossômico 16S/genética , Rhizobium/genética , Rhizobium/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
By the methods of emergy, this paper studied the input and output, working efficiency, and environmental loading of the agro-ecolomic system in Shanxi Province in 2005. The results showed that in 2005, the agro-ecolomic system in Shanxi still stayed in the period of traditional agriculture, which mainly depended on manpower and environmental resources. The emergy investment ratio (EIR) was 1.07, emergy yield ratio (EYR) was 0.99, and environmental loading ratio (ELR) was 6.55. In the structural adjustment of agriculture, stockbreeding had made great strides forward, but grain crops other than rice and wheat, vegetables, and fruits still had smaller emergy yield and were far from becoming dominant industry. The efficiency of the agro-ecolomic system was lower, and its environmental loading press was bigger. For the future, the surplus labors in agriculture in Shanxi should be shifted continually to other industries, and the high quality emergy such as agricultural science and technology should be increased to improve the use efficiency of environmental resources and the input and output of the system emergy.