Lactobacillus salivarius Ameliorates AFB1-induced hepatotoxicity via PINK1/Parkin-mediated mitophagy in Geese.

Aflatoxin B1 (AFB1) is commonly found in feed ingredients and foods all over the world, posing a significant threat to food safety and public health in animals and humans. Lactobacillus salivarius (L. salivarius) was recorded to improve the intestinal health and performance of chickens. However, whether L. salivarius can alleviate AFB1-induced hepatotoxicity in geese was unknown. A total of 300 Lande geese were randomly assigned to five groups: control group, AFB1 low-dose group (L), L. salivarius+AFB1 low-dose group (LL), AFB1 high dosage groups (H), L. salivarius+AFB1 high dosage groups (LH), respectively. The results showed that the concentrations of ALT, AST, and GGT significantly increased after exposure to AFB1. Similarly, severe damage of hepatic morphology was observed including the hepatic structure injury and inflammatory cell infiltration. The oxidative stress was evidenced by the elevated concentrations of MDA, and decreased activities of GSH-Px, GSH and SOD. The observation of immunofluorescence, real-time PCR, and western blotting showed that the expression of PINK1 and the value of LC3II/LC3I were increased, but that of p62 significantly decreased after AFB1 exposure. Moreover, the supplementation of L. salivarius effectively improved the geese performance, ameliorated AFB1-induced oxidative stress, inhibited mitochondrial mitophagy and enhanced the liver restoration to normal level. The present study demonstrated that L. salivarius ameliorated AFB1-induced the hepatotoxicity by decreasing the oxidative stress, and regulating the expression of PINK1/Parkin-mediated mitophagy in the mitochondria of the geese liver. Furthermore, this investigation suggested that L. salivarius might serve as a novel and safe additive for preventing AFB1 contamination in poultry feed.

Significant association of mutations close to LCORL gene with growth performance in Zhedong white geese (Anser cygnoides).

This study aimed to investigate the role of the LCORL gene in regulating the growth performance of Zhedong white (ZDW) geese, belonging to the swan geese (Anser cygnoides), and identify possible selective signatures in diverse goose breeds. Single nucleotide polymorphisms around LCORL were genotyped, and their associations with body-size-related (BSR) traits were estimated. The results showed that the genotyped loci upstream of LCORL were significantly related to the body weight and breast width of ZDW geese aged 10 weeks (p < 0.05). A genome scan comparing expected heterozygosity among different breeds identified a ~150 kb long genomic region with extremely low heterozygosity downstream of LCORL among swan geese. Further, significant associations of variants within the low heterozygosity region among ZDW geese with BSR traits, including body weight, body length and breast width (p < 0.05) were also detected. Overall, mutations adjacent to LCORL were related to the growth performance of swan geese, and the significant effects of variants in a low-heterozygosity region on BSR traits provided valuable insights into the molecular mechanism of artificial selection reshaping body stature in swan geese.

Expression profile and the G63A mutation of IGF2 gene associated with growth traits in Zhedong-White goose.

Insulin-like growth factors 2 (IGF2) is an insulin-like growth factor that plays a major role in animal growth, cell proliferation and differentiation, as well as reproduction. IGF2 is well-known to be a candidate gene of growth and reproductive traits in many agricultural animals. Our previous study revealed that the G63A (Chr2: G26541617A) mutation within IGF2 exon 1 showed a significant association with egg numbers (E180d) of Sanhua goose population (p < 0.05). However, little work focus on the correlation between the IGF2 mutations and goose growth traits. In this study, qPCR indicated that the IGF2 mRNA highly expressed in leg muscle, liver, ovary and pituitary gland. Meanwhile, association analysis showed that the G63A mutation was significantly associated with the body weight of first-hatched Zhedong-White geese (BW0, p < 0.05), and strongly significantly associated with the BW2, BW4, BW6, BW8 and BW10 (p < 0.01). The GG homozygous had the lowest BW (from 4 weeks to 10 weeks) than those of AA and AG genotypes (p < 0.01), and the allele A was also positively correlated with the BW of the Zhedong-White goose population. Therefore, the G63A mutation in IGF2 may be an important genetic marker for goose breeding.

Insulin-like growth factor 2 (IGF2) gene: molecular characterization, expression analysis and association of polymorphisms with egg production traits in goose (Anser cygnoides).

Insulin-like growth factor 2 (IGF2) belongs to the member of the insulin-like growth factors family, which plays key roles in animal growth, differentiation and proliferation, as well as reproduction and the regulation of ovarian follicle development. However, little is known about the goose IGF2 gene. In this study, a 1879 bp fragment that covered the complete coding region (CDS) of goose IGF2 cDNA was identified for the first time. The cDNA consists of an open reading frame of 574 nucleotides with the capacity to encode a prepro-IGF-II protein of 187 amino acids, which comprises a signal peptide (24 residues), IGF-II peptide (67 residues), and C-terminal peptide (96 residues), and is closely related to that of chicken. qPCR indicates that the goose IGF2 mRNA is differentially expressed in all examined tissues of fertilized eggs (28 days) and laying Zhedong White geese (270 days). Two novel single nucleotide polymorphisms (SNPs) were detected in exon 1 (G63A, Chr2: G26541617A) and intron 1 (G38A, Chr2: G26541479A) regions, and the synonymous mutation G63A showed a significant association with egg numbers (E180d) of Sanhua goose population (p < 0.05). All the information derived from this study could be valuable and facilitate further studies on the functions of goose IGF2 gene.

The Risk Factors for Developing Clustered Vertebral Compression Fractures: A Single-Center Study.

OBJECTIVE: Vertebral compression fractures (VCFs) are common among elderly individuals, but clustered VCFs (C-VCFs) are rare and more severe. The risk factors for C-VCFs remain unclear. Thus, we investigated the clinical characteristics of C-VCFs to identify the imminent fracture risk and improve the treatment for such patients. METHODS: We reviewed the records of patients with VCF at a single medical center between January 2011 and September 2020. Patients who had 4 or more VCFs within 1 year were categorized into the C-VCF group, and the remaining patients were paired into the control group at a ratio of 2:1. We collected demographic, clinical, laboratory, and radiologic information regarding these patients. Univariate analyses, stratified analyses, and multivariate logistic regression were performed to identify the risk factors for C-VCFs. RESULTS: A total of 156 patients were enrolled, of whom 52 were patients with C-VCF. Patients with C-VCF had more severe fractures and pain, with fractures occurring at uncommon sites of the spine. The independent risk factors for C-VCFs included glucocorticoid (GC) treatment (P < .001; hazard ratio [HR], 12.7), recent fracture history (P = .021; HR, 5.5), and lower trabecular bone score (TBS) (P = .044; HR, 1.6). TBS and bone mineral density had greater predictive values in patients without GC treatment (P < .001). Sex, age, and bone turnover biomarkers were not independent risk factors for C-VCFs. CONCLUSION: C-VCFs are rare adverse consequences of severe osteoporosis, for which GC treatment, recent fracture history, and lower TBS are unique risk factors that are valuable for the early identification and prevention of C-VCFs.

Photo-electrochemical Osmotic System Enables Simultaneous Metal Recovery and Electricity Generation from Wastewater.

Global depletion of natural resources provides an impetus for developing low-cost, environmentally benign technologies for the recovery of valuable resources from wastewater. In this study, we present an autonomous photo-electrochemical osmotic system (PECOS) that can recover a wide range of metals from simulated metal-laden wastewater with sunlight illumination while generating electricity. The PECOS comprises a draw solution chamber with a nickel nanoparticle-functionalized titanium nanowire (Ni-TiNA) photoanode, a feed solution chamber containing synthetic wastewater with an immersed carbon fiber cathode, and a forward osmosis (FO) membrane mounted between the chambers as a separator. Using a Na2-EDTA anolyte as a draw solution at neutral pH, we demonstrate that a sunlit PECOS achieves copper recovery at a rate of 51 g h-1 per m-2 of membrane area from simulated copper-laden wastewater while simultaneously producing a maximum power density of 228 mW m-2. Moreover, because of the osmotic pressure difference generated by the photo-electrochemical reactions, the PECOS reduces the wastewater volume by extracting fresh water through the FO membrane at a water flux of 0.84 L m-2 h-1. We further demonstrate the feasibility of the PECOS in recovering diverse metals from a simulated metal-laden industrial wastewater under sunlight irradiation. Our proof-of-concept PECOS prototype provides a sustainable technological solution that leverages sunlight in an electrochemical osmotic system to recover multiple resources from wastewater.

Low urinary iodine is a protective factor of central lymph node metastasis in papillary thyroid cancer: a cross-sectional study.

BACKGROUND: An abrupt increase of thyroid cancer has been witnessed paralleling the supplemented iodine intake in formerly iodine-deficient countries. And increased iodine intake has been linked to the rising incidence rate of papillary thyroid cancer (PTC). However, the correlation between iodine and clinicopathological features of PTC has not been well-characterized. This study aimed to investigate the associations between iodine intake and the clinicopathological features of PTC patients. METHODS: Three hundred and fifty-nine PTC patients who received surgical treatment in Peking Union Medical College Hospital from May 2015 to November 2020 were retrospectively reviewed. The associations between urinary iodine (UI), urinary iodine/creatinine ratio (UI/U-Cr), and the clinicopathological features of PTC were analyzed. Univariate and multivariate analysis were performed to investigate the relationship between UI level and central lymph node metastasis (CLNM). RESULTS: There were no significant differences in UI in different groups according to the variables studied, except that patients with CLNM had higher UI level than CLNM(-) patients. No associations were found between UI/U-Cr and clinicopathological features except variant subtypes (classic/follicular). After dividing patients into high-iodine group and low-iodine group, more patients were found to have CLNM in the high-iodine group (p = 0.02). In addition, younger age, larger tumor size, and classic variant were positively correlated with CLNM (p < 0.05). Univariate analysis showed that insufficient iodine intake (≤ 99 µg/L) was associated with decreased CLNM risk in PTC. And after defining insufficient iodine intake as ≤ 109 µg/L and above requirements as ≥ 190 µg/L, multivariate analysis showed that lower iodine was associated with CLNM in total population of PTC (OR 0.53, 95% CI 0.31-0.91) and in PTC < 1 cm (papillary thyroid microcarcinoma, PTMC) (OR 0.43, 95% CI 0.21-0.87). CONCLUSIONS: Low iodine was a protective factor for CLNM in papillary thyroid cancer, particularly in those < 1 cm. These results indicated that iodine may not only be an initiator of tumorigenesis, but also a promoter of the development of PTC.

Valorization of manganese-containing groundwater treatment sludge by preparing magnetic adsorbent for Cu(II) adsorption.

Mn-containing sludge from groundwater treatment was converted to magnetic particles (MPs) via a one-step hydrothermal method using sodium ascorbate (SA) as the reductant. The MPs were characterized by X-ray diffraction spectroscopy, Mössbauer spectroscopy, X-ray fluorescence spectroscopy, magnetometry and Gran titration and the results showed that magnetic jacobsite was obtained as an intermediate product in transformation of Fe/Mn oxides to siderite and rhodochrosite. When the molar ratio of SA to Mn in the sludge was two, the produced MPs-2 contained a mixture of ferrihdyrite, hematite, jacobsite and Si/Al oxides, and could magnetize at 2.4 emu/g. Ferrihydrite content in MPs decreased with increase of the SA/Mn molar ratio, leading to decrease of the surface sites concentration (Hs). Thus, MPs-2 contained optimized Hs of 6.7 mmoL/g and a desirable adsorption capacity of Cu(II) (73.1 mg/g). The adsorption isotherms of MPs-2 on Cu(II) complied with the Langmuir model and the adsorption kinetics fitted well with the pseudo-second-order model. The major mechanism of adsorption was cationic exchange of the coordinated H and Na ions on MPs-2 surface sites with the Cu(II) ions. This study was the first time to report preparation of MPs by recycling Mn-containing sludge, which could be used as a high-capacity and low-cost adsorbent in treatment of heavy metal-containing wastewater.

The prognostic impact of primary tumor resection in pancreatic neuroendocrine tumors with synchronous multifocal liver metastases.

BACKGROUND: Whether primary tumor resection benefits patients with synchronous multifocal liver metastases from pancreatic neuroendocrine tumors remains controversial. We investigated whether primary tumor resection significantly affects survival in this study. METHODS: A retrospective study of patients with synchronous multifocal liver metastases from pancreatic neuroendocrine tumors between 1998 and 2016 was performed. Patient demographics, operation details, adjuvant treatment, and pathological and survival information were collected, and relevant clinical-pathological parameters were assessed in univariate and multivariate survival analyses. RESULTS: Sixty-three patients were included in this study, including 35 who underwent primary tumor resection. The median survival time and 5-year survival rate of this cohort were 50 months and 44.5%, respectively. Median survival time in the resected group was significantly longer at 72 months than that of 32 months in the nonresected group (p = 0.010). Multivariate analysis showed that primary tumor surgery was a significant independent prognostic factor (HR 0.312, 95% CI: 0.128-0.762, p = 0.011). CONCLUSIONS: Primary tumor resection significantly benefits patients with synchronous multifocal liver metastases from pancreatic neuroendocrine tumors.

Surgery in overweight patients with insulinoma: effects on weight loss.

OBJECTIVE: Weight loss induced by the complete resection of insulinoma is controversial in overweight patients. The study sought to explore postoperative weight loss and metabolic changes in overweight insulinoma patients. METHODS: A retrospective study was conducted to review the follow-up data of insulinoma patients with a BMI ≥25kg/m2 who underwent complete lesion resection between May 2010 and May 2015. Body mass index (BMI), weight loss (WL) and percentage weight loss (%WL) were main outcomes. RESULTS: Fifty-one patients were included with a median follow-up of 28 months. The BMI at 3 months, 1 year, 2 years and 3 years postoperatively were significantly lower than the preoperative BMI values (p < .01). The WL% was 12.9% at 3 months postoperatively without significant changes throughout the 3-year follow-up. WL and the %WL were significantly higher in the high BMI group (BMI≥ 27.5 kg/m2). Multivariate analysis indicated that higher initial BMI was associated with increased weight loss (p = .001). 63.8% of patients with hypertension recovered and improved sleep quality was evident in all patients with obstructive sleep apnea syndrome within 1 year postoperatively. CONCLUSIONS: Weight significantly decreased postoperatively in overweight insulinoma patients, which was more evident in patients with higher BMI and metabolic comorbidities were largely improved.

Occurrence and formation potential of nitrosamines in river water and ground water along the Songhua River, China.

The presence of mutagenic and carcinogenic nitrosamines in water is of great concern. In this study, seven nitrosamines including N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosomethylethylamine (NMEA), N-nitrosopyrrolidine (NPyr), N-nitrosopiperidine (NPip), N-nitrosodi-n-propylamine (NDPA), and N-nitrosodi-n-butyl-amine (NDBA) were investigated in river water and ground water samples collected from 5 representative cities (Jilin, Songyuan, Harbin, Jiamusi and Tongjiang) along the Songhua River. The total concentrations of nitrosamines in ground water were n.d. (not detected) to 60.8ng/L, NDMA was the most frequently detected nitrosamines in ground water, followed by NDEA and NPip. Relatively high detected frequency and concentrations of NDMA were also observed in river water samples, and the total nitrosamines' concentration at midstream is always higher than that at upstream and downstream. After 24hr chlorination, concentration of NDMA, NDBA was obviously increased but NDEA was reduced. Furthermore, UV254 showed a better relationship with NDMA-FP rather than dissolved organic carbon (DOC), NH4-N, and TDN.

The protective effects of Lactobacillus SNK-6 on growth, organ health, and intestinal function in geese exposed to low concentration Aflatoxin B1.

Aflatoxin B1 (AFB1) is a prevalent mycotoxin present in feed ingredients. In this study, we investigated the effects of Lactobacillus salivarius (L. salivarius) on the Landes geese exposed to AFB1. The 300 one-day-old Landes geese were randomly divided into five groups: The control group received a basic diet, while the other groups were fed a basic diet supplemented with 10 µg/kg AFB1, 10 µg/kg AFB1+ 4*108 cfu/g L. salivarius, 50 µg/kg AFB1, and 50 µg/kg AFB1 + 4*108 cfu/g L. salivarius for 63 d. Results showed that high level AFB1 exposure significantly decreased final BW and ADG, increased feed/gain ratio (F/G) and liver index (P < 0.05). L. salivarius improved levels of IL-1, IL-6, and IL-12 under low level of AFB1 exposure (P < 0.05), along with similar trends observed in serum IgA, IgG, IgM, T3, T4, TNF-ɑ, and EDT (P < 0.05). AFB1 exposure reduced jejunum villus high and villus high/crypt depth ratio, and suppressed expression of ZO-1, Occludin, and Claudin-1 mRNA, and significant improved with L. salivarius supplementation under low level AFB1 exposure (P < 0.05). AFB1 significantly increased expression levels of TLR3 and NF-kB1, with supplementation of L. salivarius showing significant improvement under low AFB1 exposure (P < 0.05). Cecal microbiota sequencing revealed that under low level AFB1 exposure, supplementation with L. salivarius increased the abundance of Bacteroidetes and Lactococcus. In summary, supplementation with 4*108 cfu/g L. salivarius under 10 µg/kg AFB1 exposure improved growth performance and immune capacity, enhanced jejunum morphology, reduced liver inflammation, altered the cecal microbial structure, and positively affected the growth and development of geese.

The impact of replacing corn with elephant grass (Pennisetum purpureum) on growth performance, serum parameters, carcass traits, and nutrient digestibility in geese.

This study investigated the effects of partially replacing corn with elephant grass dry matter (air drass) on growth performance, serum parameters, carcass traits, and nutrient digestibility in geese. A total of 360 one-day-old Hortobágyi geese were randomly divided into three groups: control (basic diet), 12 % elephant grass, and 24 % elephant grass. The geese were raised for 70 days. The results showed that compared to the control, 12 % elephant grass had no adverse effects on final body weight, feed/gain ratio, mortality, serum liver and kidney function markers. However, 24 % elephant grass significantly reduced the final body weight (P < 0.001) and feed/gain ratio (P = 0.026) compared to the control group. Both experiment groups had decreased serum aspartate aminotransferase (P < 0.001), alanine aminotransferase (P < 0.001), alkaline phosphatase (P < 0.001), triglycerides (P < 0.001), and total cholesterol (P < 0.001). The addition of 12 % and 24 % elephant grass reduced abdominal fat (P = 0.002), but it had no significant effect on slaughter rate, half-bore rate, full-bore rate, breast muscle rate and leg muscle rate. For nutrient digestibility, 12 % elephant grass improved neutral detergent fiber digestibility compared to the control group (P = 0.026). The 24 % grass group had reduced Ca absorption (P = 0.020). Overall, the findings suggest that partially replacing corn with 12 % elephant grass in goose diet can maintain growth performance and carcass traits.It also has no negative effect on nutrient digestibility while improving serum parameters.

A cross-species transcriptomic analysis reveals a novel 2-dimensional classification system explaining the invasiveness heterogeneity of pancreatic neuroendocrine tumor.

Pancreatic neuroendocrine tumors (PanNETs), the second most common type of primary pancreatic tumors, display notable heterogeneity in invasiveness. Current knowledge regarding genomic alterations, including DAXX/ATRX, MEN1 mutations, and copy number variations (CNVs), provides some insights into tumor invasiveness. However, the underlying reasons for the significant variation in invasiveness between insulinoma and other types of PanNETs remain unclear. To construct a comprehensive model for the stratification of prognosis, we employed analysis of both the well-established Rip1-Tag2 (RT2) mouse model of PanNETs and human PanNETs with various functional types. Firstly, by applying single-cell and bulk RNA sequencing in PanNETs from different ages and strains of RT2 mice and human PanNETs, we introduced a 2-dimensional (2D) classification system. Based on the 2-D classification system, human PanNETs were mainly classified as benign insulinomas or non-insulinomas subclusters. Non-insulinomas subtypes mainly included gastrinomas, glucagonomas, VIPomas, and NF-PanNETs, which all exhibited potential invasiveness. In addition, we discovered an enrichment of specific CNV patterns and mutations in corresponding human PanNET subclusters. Then we denoted somatic DAXX/ATRX as the 'second hit' and confounding factors for invasiveness. Finally, by combining the 2D system, DAXX/ATRX mutation status, and tumor diameter, a group of indolent PanNETs with minimal recurrence risk was identified. In conclusion, our current work constructed a comprehensive model to elucidate the heterogeneity of invasiveness in PanNETs and improve prognostic stratification.

More resilient polyester membranes for high-performance reverse osmosis desalination.

Thin-film composite reverse osmosis membranes have remained the gold standard technology for desalination and water purification for nearly half a century. Polyamide films offer excellent water permeability and salt rejection but also suffer from poor chlorine resistance, high fouling propensity, and low boron rejection. We addressed these issues by molecularly designing a polyester thin-film composite reverse osmosis membrane using co-solvent-assisted interfacial polymerization to react 3,5-dihydroxy-4-methylbenzoic acid with trimesoyl chloride. This polyester membrane exhibits substantial water permeability, high rejection for sodium chloride and boron, and complete resistance toward chlorine. The ultrasmooth, low-energy surface of the membrane also prevents fouling and mineral scaling compared with polyamide membranes. These membranes could increasingly challenge polyamide membranes by further optimizing water-salt selectivity, offering a path to considerably reducing pretreatment steps in desalination.

Aquifer clogging caused by chlorine disinfection during the reclaimed water recharge.

Aquifer clogging plays a critical role in the efficiency of reclaimed water recharge. While chlorine disinfection is commonly used for reclaimed water, its impact on clogging has seldom been discussed. Thus, this study aimed to investigate the mechanism of chlorine disinfection on clogging by establishing a lab-scale reclaimed water recharge system that utilized chlorine-treated secondary effluent as feed water. The findings indicated that increasing the chlorine concentration led to a surge in the total amount of suspended particles, and the median particle size increased from 2.65 µm to 10.58 µm. Furthermore, the fluorescence intensity of dissolved organic matter decreased by 20%, with 80% of these compounds, including humic acid, becoming entrapped within the porous media. Additionally, the formation of biofilms was also found to be promoted. Microbial community structure analysis unveiled a consistent dominance of Proteobacteria consistently exceeded 50% in relative abundance. Moreover, the relative abundance of Firmicutes increased from 0.19% to 26.28%, thereby verifying their strong tolerance to chlorine disinfection. These results showed that higher chlorine concentrations could stimulate microorganisms to secrete an increased quantity of extracellular polymeric substance (EPS) and form a coexistence system with the trapped particles and natural organic matter (NOM) within the porous media. Consequently, this supported the formation of biofilms, thereby potentially elevating the risk of aquifer clogging.

Bile acids supplementation modulates lipid metabolism, intestinal function, and cecal microbiota in geese.

Bile acids(BAs) are important components of bile and play a significant role in fat metabolism. However, there is currently no systematic evaluation of the use of BAs as feed additives for geese.This study aimed to investigate the effects of adding BAs to goose feed on growth performance, lipid metabolism, intestinal morphology, mucosal barrier function, and cecal microbiota. A total of 168 28-day-old geese were randomly assigned to four treatment groups and fed diets supplemented with 0, 75, 150, or 300 mg/kg of BAs for 28 days. The addition of 75 and 150 mg/kg of BAs significantly improved the feed/gain (F/G) (p < 0.05).The addition of BAs decreased abdominal fat percentage and serum total cholesterol (TC) levels, with 150 mg/kg of BAs significantly reducing serum triglyceride levels and increased expression of Farnesoid X Receptor (FXR) mRNA in the liver(p < 0.05), 300 mg/kg of BAs significantly increasing the expression level of liver peroxisome proliferator-activated receptor α (PPARα) (p < 0.05). In terms of intestinal morphology and mucosal barrier function, 150 mg/kg of BAs significantly increased villus height (VH) and VH/crypt depth (CD) in the jejunum (p < 0.05). The addition of 150 and 300 mg/kg of BAs significantly reduced the CD in the ileum, while increasing VH and VH/CD (p<0.05). Additionally, the addition of 150 and 300 mg/kg of BAs significantly increased the expression levels of zonula occludens-1 (ZO-1) and occludin in the jejunum. Simultaneously 150mg/kg and 300mg/kg BAs increased the total short-chain fatty acids (SCFA) concentrations in the jejunum and cecum(p < 0.05).Supplementation with BAs resulted in a significant increase in the ɑ-diversity of cecal microbiota and a decrease in the abundance of Proteobacteria in the cecum. The addition of 150 mg/kg of BAs significantly reduced the abundance of Bacteroidetes and increased the abundance of Firmicutes. Moreover,Linear discriminant analysis Effect Size analysis (LEfSe) showed that the abundances of bacteria producing SCFA and bile salt hydrolases (BSH) were increased in the BAs-treated group. Furthermore, Spearman's analysis showed that the genus Balutia, which is negatively correlated with visceral fat area, was positively correlated with serum high-density lipoprotein cholesterol (HDL-C), while Clostridium was positively correlated with intestinal VH and VH/CD. In conclusion, BAs can be considered an effective feed additive for geese, as they increased SCFA concentration, improve lipid metabolism and intestinal health by enhancing the intestinal mucosal barrier, improving intestinal morphology, and altering the cecal microbiota structure.

Feeding whole-plant ensiled corn stover affects growth performance, blood parameters, and Cecal microbiota of Holdobagy goose.

Introduction: The aim of this study was to investigate the effects of adding whole-plant ensiled corn stalks (WECS) to the diet of Holdorbagy geese on their growth performance, serum parameters, and cecal microbiota. Geese farming is an important agricultural practice, and optimizing their diet can contribute to better growth and health outcomes. However, there is limited research on the utilization of WECS as a feed source for geese. Understanding the potential effects of WECS on growth, blood parameters, and cecal microbiota can provide valuable insights into its feasibility and impact on geese farming practices. Methods: A total of 144 six-week-old Holdorbagy geese were randomly assigned to one of three groups: a control group (0% WECS), a group fed 15% WECS and 85% concentrated feed (15% WECS), and a group fed 30% WECS and 70% concentrated feed (30% WECS). The trial period lasted for three weeks, during which the growth performance, serum parameters, and cecal microbiota were assessed. Results: The results revealed significant findings in different aspects. Firstly, the feed-to-gain ratio (F/G ratio) of the 15% WECS group was significantly higher than that of the control group (p<0.05), indicating potential challenges in feed efficiency. Additionally, the average daily feed intake (ADFI) of both the 15% and 30% WECS groups was significantly higher than that of the control group (p<0.05), suggesting increased appetite or palatability of the diet containing WECS. In terms of serum parameters, the level of lactate dehydrogenase (LDH) in the 30% WECS group was significantly lower than that in the control group (p<0.05). Moreover, there was a tendency for increasing Fe levels and decreasing Zn levels with higher levels of WECS supplementation, although the differences were not statistically significant (p<0.05). Furthermore, the principal coordinate analysis showed significant differences in the composition of cecal microbiota among the three groups (p < 0.01). The observed_species, Shannon, and Pielou_e indices of the 30% WECS group were significantly higher than those of the 0% and 15% WECS groups (p<0.05), while the Simpson index of the 15% WECS group was significantly lower than that of the control group (p<0.05). Discussion: The results indicate that the addition of WECS to the geese diet has both positive and negative effects. The study suggests that WECS can be a long-term stable feed source for geese, which can contribute to reducing feeding costs. However, it is important to monitor the amount of WECS added as it can affect the absorption of Zn by geese. Supplementation of Zn in the diet might be necessary to meet the needs of geese. Notably, adding 30% WECS to the diet can increase the richness, evenness, and diversity of the cecal microbiota, indicating potential benefits to gut health. In conclusion, this study highlights the potential of WECS as a feed source for geese. It provides valuable insights into the effects of WECS on growth performance, serum parameters, and cecal microbiota. These findings contribute to optimizing geese farming practices, improving feed utilization, and enhancing overall productivity and well-being of geese. Further research is needed to determine the optimal inclusion level of WECS and to explore strategies for mitigating any negative effects.

Deciphering the influence of multiple anthropogenic inputs on taxonomic and functional profiles of the microbial communities in Yitong River, Northeast China.

We conducted the analysis of physicochemical parameters, 16S rRNA amplicon sequencing and real-time quantitative polymerase chain reaction to explore the impact of human inputs on the bacterioplankton communities within a tributary of the largest river flowing through a megacity in northeast China. Agriculture largely accounted for the alteration of diversity and functions of the microbial communities. Furthermore, nitrate and total phosphorus declined at the reservoir outlet. The WWTP effluent discharge caused a decrease of the relative abundance of Actinobacteria and Cyanobacteria, while the impact on the variation of alpha diversity of river microbial community was slight. Carbon fixation and nitrogen cycle varied with the change of land use type. The rare taxa contributed with a predominant role in the response to environmental variables and NH3-N as well as NO3--N were the main environmental factors that drove the shift in the bacterial community. The occurrence of the human-specific fecal indicator was mostly derived from agriculture, and its increase in relative abundance was observed in the WWTP effluent. Thus, our study provides guidance for ecological assessment and management of rivers by revealing the response pattern of river bacterioplankton to multiple types of anthropogenic stressors.

Oxidation of phthalate acid esters using hydrogen peroxide and polyoxometalate/graphene hybrids.

Phthalate acid esters (PAEs) have been adsorbed and oxidatively degraded into small molecules including lactic acid (LA), formic acid (FA), H2O and CO2 using polyoxometalates (POMs)/graphene hybrids. We demonstrated that super-lower concentrations of PAEs could be oxidized, which was due to their unique structure. POM molecules have been embedded onto graphene to form H5PMo10V2O40@surfactant(n)/Graphene(L wt%) (abbreviated as HPMoV@Surf(n)/GO(L wt%)) using surfactants with the carbon chain length n = 2, 4, 6 and 8 for the loading of HPMoV. The coexistence of the graphene and surfactant layer (on HPMoV@Surf(n)/GO(20 wt%)) adsorbed PAE molecules and transported them rapidly to HPMoV active sites. And n values determined the electron transfer ability between graphene and POMs that promoted PAEs oxidation. The loading of POMs on the surface of graphene permitted HPMoV@Surf(n)/GO(L wt%) act as interfacial catalyst which degraded various PAEs (i.e., diethyl phthalate (DEP), diallyl phthalate (DAP) and di (2-ethylhexyl) phthalate (DEHP)) while removed more than 70% of TOC and COD. The degradation of DEP achieved 93.0% with HPMoV@Surf(n)/GO(20 wt%) and H2O2, which followed first-order kinetics and the reaction activation energy (Ea) of 23.1 kJ/mol. Further, HPMoV@Surf(n)/GO(20 wt%) showed potential for the removal of PAEs in Wastewater Treatment Plant (WWTP), and the degradation efficiency for PAE (DEP) in secondary effluent achieved 55.0%. In addition, the loading method for POMs on graphene eliminated the leaching of POMs from graphene, and the degradation efficiency could still reach 88.1% after ten recycles.