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Extending the voluntary waiting period (VWP) for insemination in dairy cows is of interest to reduce the frequency of calving events and inseminate at a moment with fewer fertility problems. Little is known about the calves born from dams with a different VWP followed by a different calving interval (CInt). The objective of the current study was to identify the effect of dam's CInt on body condition, metabolic status, and milk production of their offspring from birth till 100 DIM of the offspring's first lactation. Holstein Friesian dairy cows (n = 154, 41 primiparous, 113 multiparous) were blocked according to parity, milk yield, and somatic cell count (SCC), and randomly assigned to a VWP of 50, 125, or 200 d. Female calves (n = 62) from cows with different CInt were monitored from birth until their first calving event as heifer. Certain dams were not successfully inseminated soon after the planned VWP, resulting in differences between the intended VWP and the actual CInt. Calves were regrouped according to their dam's actual CInt (CInt_1: 324 - 408 d; CInt_2: 409 - 468 d; CInt_3: 469 - 586 d). The dam's CInt did not affect calf birth weight. From birth to weaning, the calves born to dams in CInt_1 (0.34 mmol/L (confidence interval (CI): 0.30, 0.37) had a higher plasma nonesterified fatty acids (NEFA) concentration than CInt_2 (0.28 mmol/L (CI: 0.26, 0.31)) and CInt_3 (0.26 mmol/L (CI: 0.24, 0.29)) calves. Calves born to dams with a shorter CInt (CInt_1) had greater IgG and IgM against keyhole limpet hemocyanin (KLH) than CInt_3 (IgG: 6.05 ± 0.30 vs. 4.64 ± 0.30; IgM: 6.45 ± 0.17 vs. 5.89 ± 0.16, respectively) before weaning. After weaning till calving, CInt_1-calves tended to have greater plasma NEFA concentration than CInt_3-calves. During the first 100 d in milk, a longer CInt of the dams resulted in lower plasma IGF_1 (CInt_2), lower milk lactose (CInt_3) and fat and protein corrected milk (FPCM) (CInt_2) in offspring, compared with shorter CInt of the dams (CInt_1). Collectively, a longer CInt in dams did not affect birth weight of their calves or body weight during the weaning or rearing phase. From birth till weaning, a longer CInt in dams resulted in less IgG against KLH and lower plasma NEFA concentration in plasma of the calves. During the first lactation of their offspring, a longer CInt in dams can result in a lower plasma IGF_1 and FPCM during the first 100 DIM, although effects were not present in all CInt categories.
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Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-lactation dairy cows. Twenty-four multiparous Holstein cows in early lactation were randomly allocated to control (CON), low-RPG (LRPG), medium-RPG (MRPG), or high-RPG (HRPG) groups in a randomized block design. The cows were fed a basal total mixed ration diet with 0, 200, 350, and 500 g of RPG per cow per day, respectively. Rumen fluid samples were analyzed using Illumina MiSeq sequencing and ultrahigh-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. MRPG supplementation increased bacterial richness and diversity, including increasing the relative abundance of cellulolytic bacteria, such as Ruminococcus, Lachnospiraceae_NK3A20_group, Ruminiclostridium, and Lachnospiraceae_UCG-008 MRPG significantly increased the concentrations of acetate, propionate, butyrate, and total volatile fatty acid in the rumen. Ruminal fluid metabolomics analysis showed that RPG supplementation could significantly regulate the synthesis of amino acids digested by protozoa in the rumen. Correlation analysis of the ruminal microbiome and metabolome revealed some potential relationships between major bacterial abundance and metabolite concentrations. Our analysis found that RPG supplementation of different doses can change the diversity of microorganisms in the rumen and affect the rumen fermentation pattern and microbial metabolism and that a daily supplement of 350 g of RPG might be the ideal dose.IMPORTANCE Dairy cows in early lactation are prone to a negative energy balance because their dry matter intake cannot meet the energy requirements of lactation. Rumen-protected glucose is used as an effective feed additive to alleviate the negative energy balance of dairy cows in early lactation. However, one thing that is overlooked is that people often think that rumen-protected glucose is not degraded in the rumen, thus ignoring its impact on the microorganisms in the rumen environment. Our investigation and previous experiments have found that rumen-protected glucose is partially degraded in the rumen. However, there are few reports on this subject. Therefore, we conducted research on this problem and found that rumen-protected glucose supplementation at 350 g/day can promote the development and metabolism of rumen flora. This provides a theoretical basis for the extensive application of rumen bypass glucose at a later stage.
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Suplementos Nutricionais , Glucose/farmacologia , Lactação/efeitos dos fármacos , Microbiota/efeitos dos fármacos , Rúmen/efeitos dos fármacos , Ração Animal , Animais , Bactérias/classificação , Bactérias/genética , Bovinos , Dieta/veterinária , Feminino , Fermentação , Lactação/metabolismo , Metabolômica , Microbiota/genética , RNA Ribossômico 16S , Rúmen/metabolismo , Rúmen/microbiologiaRESUMO
Background: The dentist has a responsibility to provide nutritional counseling and fluoride consumption recommendations. The purpose of this study was to measure and compare the concentrations of fluoride in a large number of alternative milk beverages and bovine milk. Study design: Thirty-three milk alternatives, including 9 diverse types and 11 different brands, were analyzed using a fluoride ion-selective electrode (ISE) and an ISE meter. Fluoride concentrations were then compared among different types and between different brands. Results: Fluoride concentration ranged from 0.01 ppm (Malk® Pure Cashew Milk) to 0.80 ppm (Almond Breeze® Original Unsweetened Almond Milk) with a mean concentration of 0.32 ppm. When compared, bovine whole milk (0.03±0.00 ppm) was found to be significantly lower in fluoride than all samples analyzed except Malk Pure Cashew Milk, Soy Milk Vanilla, Rice Milk, and Pecan Milk. Major differences also existed between the same milk alternative types of different brands. Conclusion: The amount of fluoride varies among different types of milk alternatives and different brands. To ensure that the dental team can provide proper recommendations regarding fluoride use, manufacturers should consider placing fluoride concentrations on nutrition labels.
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Fluoretos , Fluorose Dentária , Animais , Bebidas , Bovinos , LeiteRESUMO
PURPOSE: To synthesize a small library of antibacterial dental monomers based on quaternary ammonium salts and to test their antibacterial activity against cariogenic bacteria. METHODS: Five new antibacterial monomers were synthesized and characterized by NMR, IR and HRMS. RESULTS: Cytotoxicity assays using human gingival fibroblast cells showed that these new antibacterial monomers were biocompatible at concentrations of 10â»5 M and displayed less cytotoxicity than BisGMA, a common dental monomer. When analyzed in vitro, all new monomers demonstrated strong inhibitory activity against biofilm formation by cariogenic Streptococcus mutans and Lactobacillus casei. Results indicated that antibacterial monomers containing a long alkyl (i.e. hexadecyl) chain are superior to their shorter-chain counterparts. The cross-linking monomers based on glycerol dimethacrylate also consistently outperformed their monomethacrylate analogs. Finally, the ammonium salts containing the dimethylbenzyl moiety were superior to the similar structures containing 1,4-diazabicyclo[2.2.2]octane (DABCO) in some cases. CLINICAL SIGNIFICANCE: All five new monomers were deemed biocompatible at concentrations of 10â»5 M or less, and most had better biocompatibility than BisGMA. Dimethacrylate monomers 5 and 6 generally demonstrated high antibacterial activities, with the highest activity shown for the most lipophilic monomer 6, and these new antibacterial monomers have potential future application in dental composites and bonding agents.
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Antibacterianos , Materiais Dentários , Antibacterianos/farmacologia , Biofilmes , Humanos , Metacrilatos , Compostos de Amônio Quaternário , Streptococcus mutansRESUMO
A new leukocyte classification method for recognition of five types of human peripheral blood smear based on mean-shift clustering is proposed. The key idea of the proposed method is to extract the texture features of leukocytes in a visual manner which can benefit from human eyes. Firstly, some feature points are extracted in a gray leukocyte image by mean-shift. Secondly, these feature points are used as seeds of the region growing to expand feature regions which can express texture in visual mode to a certain extent. Finally, a parameter vector of these regions is extracted as the texture feature. Combing the vector with the geometric features of the leukocyte, the five typical classes of leukocytes can be recognized successfully using artificial neural network (ANN). A total number of 1 310 leukocyte images have been tested and the accurate rate of recognition for neutrophil, eosinophil, basophil, lymphocyte and monocyte are 95.4%, 93.8%, 100%, 93.1% and 92.4%, respectively, which shows the feasibility and high robustness of the proposed method.
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The fungal pathogen Candida albicans causes a variety of oral infections, including denture stomatitis, which is characterized by inflammation of the oral mucosa in direct contact with dentures and affects a significant number of otherwise healthy denture wearers. While antifungal treatment reduces symptoms, infections are often recurrent. One strategy to address this problem is to incorporate compounds with fungicidal activities into denture materials to prevent colonization. Our laboratory synthesized novel derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO), which is an organic compound typically used as a catalyst in polymerization reactions. DABCO derivatives with different aliphatic chain lengths (DC16, DC16F, DC18, and C6DC16), as well as methacrylate monomers conjugated to DABCO compounds (DC11MAF and C2DC11MAF), were synthesized and tested for antimicrobial activity. All the compounds exhibited fungicidal activity against several Candida species at concentrations ranging between 2 and 4 µg/ml. Moreover, acrylic denture base resins fabricated to contain 1, 2, or 4 wt% DABCO compounds inhibited surface C. albicans biofilm formation, as well as fungal growth, in disc diffusion assays. Remarkably, discs (4 wt%) aged for 2 months also exhibited approximately 100% growth-inhibitory activity. While some DABCO compounds exerted intermediate to high cytotoxicity against mammalian oral cell types, DC11MAF and denture base resin discs containing 2 or 4 wt% C2DC11MAF exhibited relatively low cytotoxicity against periodontal ligament (PDL) cell and gingival fibroblast (GF) lines, as well as primary oral epithelial cells. These studies demonstrate that DABCO derivatives can be incorporated into denture materials and exert fungicidal activity with minimal cytotoxicity to mammalian cells. DC11MAF and C2DC11MAF are considered strong candidates as therapeutic or preventive alternatives against Candida-associated denture stomatitis.
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Antifúngicos/farmacologia , Bases de Dentadura , Piperazinas/farmacologia , Biofilmes/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estomatite sob Prótese/microbiologiaRESUMO
A new 3D measuring method based on computer-generated moiré fringes is proposed. The two AC components of the 0-degree and 90-degree phase-shifted fringe patterns on reference plane are prepared in advance. While the AC component of the single-shot deformed pattern is multiplied by the two prepared AC components, respectively, two computer-generated moiré fringes can be retrieved. The ratio of the two computer-generated moiré fringes is just the tangent of the phase modulated by the object. It is of great potential in real-time or even dynamical 3D measurement due to its single-shot deformed pattern feature, and it avoids the influences of the object's reflectivity simultaneously. Compared to the Fourier transform profilometry, its error is smaller due to its higher first-order spectrum. Experimental results show the feasibility and validity of the proposed method.
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A novel glucose-sensitive chitosan-polyethylene oxide (CS/PEO =1:0.5~1:2.5) hydrogel with controlled release of metronidazole (MNZ) was obtained by chemical cross-linking and immobilization of glucose oxidase (GOx). The hydrogel was characterized by Fourier-transformed infrared spectroscopy (FTIR), compressive mechanical test, rheological analysis, cytotoxicity test, and antibacterial test against Porphyromonas gingivalis. The study found that the CS-PEO composite hydrogel possessed significantly better mechanical properties and biocompatibility than a single-component hydrogel. This might result from the physical cross-linking and formation of semi-interpenetrating network (semi-IPN). In addition, this novel hydrogel has self-regulate ability to release MNZ in response to the environmental glucose stimulus. Specifically, it released more drugs at higher glucose concentration, thus can lead to a greater ability to inhibit Porphyromonas gingivalis. This study has demonstrated the glucose-sensitive antibacterial hydrogel has a great potential as a new therapeutic material for treatment or prevention of periodontitis in diabetic patients.
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To achieve efficient expression and secretion of a biologically-active pullulanase, the effect of promoter and signal peptide on the production of pullulanase was studied. Three types of promoters (PP43, P apr and P amy ) and four types of signal peptides (SP sacB , SP amy , SP aprl and SP aprs ) were combined to construct twelve expression cassettes for pullulanase in Bacillus subtilis. The pullulanase activity assay was employed to quantify the level of differential expression, and a real-time PCR assay was applied to comparatively track the transcriptional level. Under the same experimental conditions, the potency ratios among the three promoters were P apr > P amy > PP43. The secretion efficiency ratios mediated by the signal peptides were SP sacB > SP amy > SP aprs > SP aprl . The highest yield of pullulanase could be achieved under the promotion mediated by P apr and secretion by SP sacB .
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Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Expressão Gênica , Glicosídeo Hidrolases/biossíntese , Glicosídeo Hidrolases/genética , Regiões Promotoras Genéticas , Sinais Direcionadores de Proteínas , Perfilação da Expressão Gênica , Glicosídeo Hidrolases/metabolismo , Transporte Proteico , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transcrição GênicaRESUMO
The enzyme FabH plays a critical role in the initial step of fatty acid biosynthesis, which is vital for the survival of bacteria. As a result, FabH has emerged as an appealing target for the development of novel antibacterial agents. In this study, employing the chemical proteomics method, we validated the previously identified skeleton amide derivatives bearing dioxygenated rings, potentially formed through metabolic processes. Building upon the proteomics findings, we then synthesized and evaluated 32 compounds containing N-heterocyclic amides for their antimicrobial activity for future optimizing the deoxygenated amides. Several compounds demonstrated potent antimicrobial properties with low toxicity, particularly compound 25, which exhibited remarkable potential as an agent with an MIC range of 1.25-3.13 µg/mL against the tested bacterial strains and an IC50 of 2.0 µM against E. coli-derived FabH. Furthermore, we evaluated nine analogues with relatively low MIC values through cytotoxicity and hemolytic activity assessments, Lipinski's rule-of-five criteria, and in silico ADMET predictions to ascertain their druggability potential. Notably, a detailed docking simulation was performed to investigate the binding interactions of compound 25 within the binding pocket of E. coli FabH, which encouragingly revealed strong binding interactions. Based on our findings, compound 25 emerges as the optimal candidate for in vivo therapy aimed at treating infected skin defects. Remarkably, the application of compound 25 demonstrated a significant reduction in the duration of wound infection and notably accelerated the healing process of infected wounds, achieving an impressive 94 % healing rate by day 10.
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Antibacterianos , Proteínas de Escherichia coli , Antibacterianos/farmacologia , Antibacterianos/química , Escherichia coli , Bactérias , Simulação de Acoplamento Molecular , Testes de Sensibilidade Microbiana , Relação Estrutura-AtividadeRESUMO
Lentiviral expression vectors encoding short hairpin RNA (shRNA) are widely used for RNAi-based gene silencing in mammalian cells. However, current methods for the construction of shRNA expression vectors require multiple steps, which are expensive, time-consuming, and error-prone. Here, we developed a single-step mixing cloning method for the generation of lentiviral shRNA expression vectors. With this method, a pair of short oligonucleotides (â¼50 nt) is required and a lentiviral shRNA vector can be constructed with only one step. This method has been used to construct 30 lentiviral shRNA expression vectors successfully.
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Clonagem Molecular/métodos , Inativação Gênica , Vetores Genéticos/genética , Lentivirus/genética , RNA Interferente Pequeno/genética , Sequência de Bases , Humanos , Proteínas Nucleares/deficiência , Proteínas Nucleares/genética , Proteínas Repressoras/deficiência , Proteínas Repressoras/genéticaRESUMO
Introduction: Negative energy balance (NEB) is the pathological basis of metabolic disorders in early lactation dairy cows. Rumen-protected glucose (RPG) is a feed additive to relieve NEB of cows in early lactation. The aims of the current study were to evaluate the impact of different doses of RPG supply on fecal microbiota and metabolome in early lactation dairy cows, and their correlation with each other. Methods: A total of 24 multiparous Holstein dairy cows in early lactation were randomly assigned to one of four treatments for the first 35 days of the early lactation period, as follows: control group, a basal diet without RPG (CON); low RPG, a basal diet plus 200 g/d RPG (LRPG); medium RPG, a basal diet plus 350 g/d RPG (MRPG); or HRPG, high RPG, a basal diet plus 500 g/d RPG (HRPG). After 35 days, fecal samples were obtained from cows in all groups individually and using 16S rRNA gene sequencing to evaluate their microbiotas, while their metabolites were evaluated through metabolomics. Results: As expected, Firmicutes and Bacteroidetes were the core bacteria phyla. After RPG supplementation, there were an increase in Firmicutes and a decrease in Bacteroidetes. MRPG increased the relative abundance of cellulolytic bacteria, including Ruminococcaceae_UCG-005, Lachnospiraceae_UCG-008, Lachnospiraceae_FCS020_group, and Ruminiclostridium_9, while it decreased the relative abundance of Alistipes, Prevotellaceae_UCG-003, and Dorea. RPG supplementation could regulate the carbohydrate metabolism and amino acid metabolism pathway significantly and relieve lipolysis in dairy cows. Correlation analysis of fecal microbiome and metabolome showed that some major differential bacteria were the crucial contributors to differential metabolites. Conclusion: In conclusion, RPG supplementation can affect the fecal microbial components and microbial metabolism, and 350 g RPG might be the ideal dose as a daily supplement.
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OBJECTIVE: The purpose of the study was to investigate the antibacterial efficacy and mechanical properties of experimental orthodontic adhesives containing newly synthesized antibacterial dimethacrylate monomers with doubly charged 1,4-diazabicyclo[2.2.2]octane (DABCO) group (dication). METHODS: Experimental orthodontic adhesives were formulated using varying compositions of synthesized antibacterial dimethacrylate monomers containing DABCO dication, C16DC2DMA and BisC11DCDMA, replacing part of the control group, Transbond™XT. The concentrations of monomers tested were 5% C16DC2DMA, 10% C16DC2DMA, 5% BisC11DCDMA, and 10% BisC11DCDMA. The biofilm-inhibition effects of the experimental adhesives against Streptococcus mutans were tested. Brackets were then bonded to extracted human teeth utilizing the experimental adhesives in the bonding protocol. The shear bond strength and modulus of elasticity of the control and experimental groups were tested. The adhesive remnant index scores were recorded. RESULTS: The experimental adhesives containing 5% or 10% BisC11DCDMA and 10% C16DC2DMA showed significantly lower S. mutans colony forming units (CFU) than the control. Both BisC11DCDMA experimental groups displayed similar mechanical properties as compared to the control, although 10% C16DC2DMA showed a reduction in shear bond strength as compared to the control. For all experimental adhesives, the adhesive remnant index scores were not significantly different compared to the control. CONCLUSION: BisC11DCDMA is a novel antibacterial dimethacrylate monomer that exhibits the significant ability to inhibit bacterial growth while maintaining acceptable mechanical properties. When incorporated into orthodontic adhesives, this monomer may reduce the occurrence of white spot lesions around brackets in orthodontic patients.
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Colagem Dentária , Braquetes Ortodônticos , Humanos , Cimentos Dentários/farmacologia , Cimentos Dentários/química , Cimentos de Resina/farmacologia , Cimentos de Resina/química , Teste de Materiais , Resistência ao Cisalhamento , Antibacterianos/farmacologiaRESUMO
This study investigated the effects of inulin on rumen fermentation parameters, ruminal microbiome and metabolites, as well as lactation performance and serum indexes in dairy cows. Sixteen Holstein dairy cows with similar body conditions were randomly divided into 2 groups (n = 8 per group), with inulin addition at 0 and 200 g/d per cow. The experiment lasted for 6 weeks, including a 1-week adaptation period and a 5-week treatment period. At the end of the experimental period, the milk, serum and rumen fluid were sampled and analyzed. The microbiome and metabolome in the rumen fluid were analyzed via 16S rRNA sequencing and untargeted metabolomics, respectively. The results showed that supplementation with inulin (200 g/d per cow) increased the milk yield (P = 0.001), milk protein (P = 0.032), lactose rate (P = 0.004) and proportion of saturated fatty acids (SFA) in milk (P < 0.001), but decreased the proportion of unsaturated fatty acids (USFA) (P = 0.041). Rumen pH (P = 0.040) and the concentration of NH3-N (P = 0.024) were decreased; however, acetate (P < 0.001), propionate (P = 0.003), butyrate (P < 0.001) and lactic acid (LA) (P = 0.043) were increased. The total cholesterol (TC) (P = 0.008) and triglycerides (TG) (P = 0.01) in serum were also reduced. Additionally, inulin addition elevated the relative abundance of several beneficial symbiotic and short-chain fatty acid (SCFA)-producing bacteria, such as Muribaculaceae (false discovery rate [FDR]-adjusted P < 0.01), Acetitomaculum (FDR-adjusted P = 0.043), and Butyrivibrio (FDR-adjusted P = 0.036), while elevating the levels of L-lysine (FDR-adjusted P = 4.24 × 10-3), L-proline (FDR-adjusted P = 0.0158), and L-phenylalanine (FDR-adjusted P = 0.027). In contrast, several pathogens and ruminal bacteria abundant in high-fat diets, such as Escherichia-Shigella (FDR-adjusted P = 0.022), Erysipelotrichaceae __UCG-004 (FDR-adjusted P < 0.01) and RF39 (FDR-adjusted P = 0.042) were decreased along with the reduction of lysophosphatidylcholine (LysoPC) (18:1 (9Z)) (FDR-adjusted P = 1.03 × 10-3), LysoPC (16:0) (FDR-adjusted P = 0.0108), LysoPC (18:2 (9Z, 12Z)) (FDR-adjusted P = 1.65 × 10-3) and 8-methylnonenoate. In conclusion, dietary inulin supplementation could increase the relative abundance of commensal microbiota and SCFA-producing bacteria, upregulate amino acidmetabolism and downregulate lipid metabolism in the rumen of dairy cows, which might further improve lactation performance and the level of serum lipids.
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A computer-assisted human peripheral blood leukocyte image classification method based on Siamese network is proposed. Firstly, a Siamese network with two identical convolutional neural network (CNN) sub-networks and a logistic regression for leukocyte five classification is designed, which can learn not only distinguishing features but also a similarity metric. Then for each category of the leukocytes, a typical sample is selected by the hematologist. To train the Siamese network, a leukocyte and a typical sample that belong to the same category make up a genuine pair and the leukocyte with the rest four typical samples respectively make up four impostor pairs. Obviously, the number of the genuine pairs is lesser than that of the impostor pairs. Thus, a data augmentation method suitable for leukocyte is used to enrich the amount of the genuine pairs. By training the Siamese network using the genuine pairs and impostor pairs, the Siamese network can not only shorten the similarity metric between the leukocyte and the same category of the typical sample but also increase the similarity metrics between the leukocyte and the different categories of the typical samples. Experimental results indicate that the proposed method can achieve 98.8% average testing accuracy. Graphical abstract.
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Processamento de Imagem Assistida por Computador/métodos , Leucócitos/citologia , Bases de Dados Factuais , Humanos , Redes Neurais de ComputaçãoRESUMO
PURPOSE: Human peripheral blood leukocytes' classification is important for diagnosing blood diseases. Many microscopic leukocyte image automatic detection methods are proposed. In recent years, convolutional neural networks (CNNs) are applied to microscopic leukocyte image automatic classification. But when a CNN is used for microscopic leukocyte image classification, the dataset's scarcity and imbalance will lead to low classification accuracy. To improve classification accuracy, a data augmentation method is proposed, and a resampling method is adopted when using a CNN method. METHODS: First, a deep CNN model for microscopic leukocyte image classification is designed. Then, a new data augmentation method based on feature concentration is proposed to enrich the dataset and overcome the problem of dataset scarcity. To make the CNN model focus on the leukocyte region, many images are generated by putting a segmented leukocyte into images with different microscopic surroundings using an image processing method. Finally, taking the imbalance of the five kinds of leukocytes in the dataset into consideration, a resampling method is adopted. The resampling method iteratively feeds the leukocyte images with a low proportion to the CNN model within an epoch to ensure that images of each of the five kinds of leukocytes are represented in relatively equal numbers in each batch. RESULTS: The experimental results demonstrate that the proposed classification method can achieve 97.6% average testing accuracy. Classification precision for the five kinds of leukocytes is above 93.4%, while sensitivity is above 92.5%. Both the proposed data augmentation and the resampling methods improve classification accuracy. CONCLUSIONS: A human peripheral blood leukocyte classification method based on a CNN and data augmentation is proposed. The problem of dataset scarcity is solved by the proposed data augmentation method, and the dataset imbalance is solved by a resampling method.
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Processamento de Imagem Assistida por Computador/métodos , Leucócitos/citologia , Redes Neurais de Computação , Humanos , MicroscopiaRESUMO
The leukocyte nucleus quick segmentation is one of the key techniques in leukocyte real-time online scanning of human blood smear. We propose a quick leukocyte nucleus segmentation method based on the component difference in RGB color space. By analyzing the captured microscopic images of the peripheral blood smears from the autoscanning microscope, it is found that the difference values between B component and G component (B - G values) in the regions of the leukocyte nuclei and the platelets are much bigger than those in the other regions, even in the regions including the stains. So, the B - G values can segment the leukocyte nuclei and the platelets with an appropriate empirical threshold because the platelets are much smaller than the leukocyte nuclei, so the leukocyte nuclei can be segmented by size filtering. Also, only an 8 bit subtraction operation is performed for the B - G values, and it can improve the leukocyte nucleus segmentation speed significantly. Experimental results show that the proposed method performs well for the five types of leukocyte segmentation with a quick speed. It is very suitable for the real-time peripheral blood smear autoscanning test application. In addition, the five types of leukocytes can be counted accurately.
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Núcleo Celular/ultraestrutura , Processamento de Imagem Assistida por Computador/métodos , Leucócitos/citologia , Contagem de Linfócitos , Reconhecimento Automatizado de Padrão , Algoritmos , Cor , Simulação por Computador , Humanos , Microscopia , Linguagens de ProgramaçãoRESUMO
OBJECTIVE: The objective of this study was to formulate experimental orthodontic bracket adhesives and test their mechanical properties, fluoride release and antibacterial activity. METHODS: Four experimental antibacterial orthodontic bracket adhesives were prepared with different compositions of synthesized antibacterial monomers replacing total 5% of dental monomers in the control Transbond XT (3M): 5%C11, 3.5%C11+1.5%C2, 5%C16, and 3.5%C16+1.5%C2. Transbond XT alone was used as control. These groups were used to bond premolar brackets to extracted premolars. Shear bond strength (SBS) was tested using an Instron machine. For antibacterial test, disk specimens (10mm diameter, 1mm thick, n=4) were fabricated and incubated with cultures of cariogenic Streptococcus mutans for 48h, and following gentle sonication, S. mutans biofilms in colony-forming-units (CFU) on the disks were enumerated by plating on agar medium. The data were analyzed using ANOVA and Tukey test (α=0.05). RESULTS: All experimental groups had similar shear bond strength (no significant difference) to the control. All experimental groups showed significant inhibitory effect against S. mutans biofilm formation, when compared to the control, but there was no significant difference between experimental groups. CONCLUSION: Antibacterial orthodontic adhesive can be fabricated to have similar mechanical properties but better caries-inhibitory effect than current adhesive.
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Colagem Dentária , Braquetes Ortodônticos , Antibacterianos , Cimentos Dentários , Análise do Estresse Dentário , Teste de Materiais , Cimentos de Resina , Resistência ao Cisalhamento , Streptococcus mutansRESUMO
Recently, a computer-generated moiré profilometry was proposed by our research group. It can effectively avoid the influence of the transient caused by moiré fringes' direct acquisition and generally owns a higher accuracy. But when the spatial spectrum of the captured deformed pattern is severely aliased caused by the measured object, the accuracy of this method may be affected to some extent due to the impure background light component extraction. So, a high precision computer-generated moiré profilometry based on background light component's accurate elimination is proposed. By adding an additional special phase-shifting sinusoidal grating to accurately extract valid information in the spatial domain and improve the sinusoidal feature of the pattern, the measurement precision can be improved effectively. Though the single-shot feature is broken, the real-time measuring feature is still maintained successfully. Experimental results show the feasibility and validity of the proposed method.
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OBJECTIVE: To evaluate the potential of various alternative milk beverages to support bacterial biofilm formation and acid production and cause unbalanced demineralization. DESIGN: in vitro assays were used to examine the ability of the beverages to support Streptococcus mutans' biofilm formation and acid production from sugar fermentation and the capacity of the beverages to buffer pH changes. Biofilm formation was done using 96-well plate model. Acid production was measured using L-Lactate assay kit, and the buffering capacity was assessed by pH titration. For ex vivo caries model, enamel and dentine slabs and S. mutans biofilms were exposed to selected alternative milk beverages three times a day, 30â¯min each, and by the end of the experiments, slab's demineralization was assessed by loss of surface microhardness. RESULTS: Of the alternative milk beverages tested in this study, Original Almond consistently supported the most S. mutans biofilms, followed by Chocolate Cashew Milk, while the least biofilms were measured with Unsweetened Flax Milk. The most acids and the lowest culture pH were measured with Toasted Coconut Almond Milk, while the least buffering capacity was measured with Unsweetened Coconut Milk. The results of ex vivo caries model showed that like Bovine Whole Milk, repeated exposure to Original Almond led to significant enamel and dentine slab demineralization, when compared to those exposed to saline as a control (Pâ¯<â¯0.001). CONCLUSIONS: These results further provide support that popular alternative milk beverages, especially those with supplemental sugars, are potentially cariogenic.