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1.
Angew Chem Int Ed Engl ; 62(28): e202304706, 2023 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-37183909

RESUMO

Chiral diarylmethylamines (DAMA) are important structural motifs widely present in pharmaceuticals, natural products, and chiral ligands. Herein, we reported a highly enantioselective synthesis of chiral DAMAs via cobalt-catalyzed enantioselective C-H alkoxylation strategy. The reaction features easy operation, the use of earth-abundant and cost-efficient cobalt(II) catalyst, and readily available ligand. A range of chiral DAMAs were efficiently synthesized in high yields with excellent enantioselectivities (up to 90 % yield and up to 99 % ee) through desymmetrization and parallel kinetic resolution. Moreover, this protocol was also compatible with the synthesis of chiral benzylamines via kinetic resolution.

2.
Angew Chem Int Ed Engl ; 62(21): e202302964, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36939152

RESUMO

Metalla-electrocatalyzed C-H oxygenation represents one of the most straightforward and sustainable approaches to access valuable oxygenated molecules. Despite the significant advances, the development of enantioselective electrochemical C-H oxygenation reaction is very challenging and remains elusive. Herein, we described the first electrochemical CoII -catalyzed enantioselective C-H alkoxylation. A broad range of enantioenriched alkoxylated phosphinamides were obtained in good yields with excellent enantioselectivities (up to 98 % yield and >99 % ee). An unusual cobalt(III) alcohol complex was prepared and fully characterized, which was proven to be a key intermediate of this C-H alkoxylation reaction. Mechanistic studies revealed that the oxidation of CoIII to CoIV was facilitated by a base and the whole process proceeded through a cobalt(III/IV/II) catalytic cycle.

3.
Angew Chem Int Ed Engl ; 62(40): e202310004, 2023 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-37585308

RESUMO

Highly efficient synthesis of axially chiral biaryl amines through cobalt-catalyzed atroposelective C-H arylation using easily accessible cobalt(II) salt and salicyloxazoline ligand has been reported. This methodology provides a straightforward and sustainable access to a broad range of enantioenriched biaryl-2-amines in good yields (up to 99 %) with excellent enantioselectivities (up to 99 % ee). The synthetic utility of the unprecedented method is highlighted by its scalability and diverse transformations.

4.
Angew Chem Int Ed Engl ; 61(38): e202210106, 2022 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-35916150

RESUMO

The past decade has witnessed a rapid progress in asymmetric C-H activation. However, the enantioselective C-H alkoxylation and amination with alcohols and free amines remains elusive. Herein, we disclose the first enantioselective dehydrogenative C-H alkoxylation and amination enabled by a simple cobalt/salicyloxazoline (Salox) catalysis. The use of cheap and readily available cobalt(II) salts as catalysts and Saloxs as chiral ligands provides an efficient method to access P-stereogenic compounds in excellent enantioselectivities (up to >99 % ee). The practicality of this protocol is demonstrated by gram-scale preparation and further derivatizations of the resulting P-stereogenic phosphinamides, which offering a flexible asymmetric alternative to access P-stereogenic mono- and diphosphine chiral ligands. Preliminary mechanistic studies on the enantioselective C-H alkoxylation reaction suggest that a cobalt(III/IV/II) catalytic cycle might be involved.


Assuntos
Cobalto , Aminação , Catálise , Ligantes , Estereoisomerismo
5.
J Gastroenterol Hepatol ; 27(10): 1629-34, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22741528

RESUMO

BACKGROUND AND AIM: Gastric adenomas (GAs) are considered as premalignant lesions of gastric adenocarcinoma. The role of Wnt signaling pathway in GAs is rarely identified. In the present study, we aimed to determine whether Wnt signaling plays a role in the pathogenesis of GAs, and to clarify the mechanism of Wnt signaling in GAs. METHODS: The study investigated the relationship between clinicopathological characteristics, Helicobacter pylori (Hp) infection, adenomatous polyposis coli (APC) promoter methylation, APC and ß-catenin immunohistochemistry expression and mutation status, compared with 38 gastric adenoma and periadenomatous tissues (PTs). RESULTS: The abnormal expression of ß-catenin in PTs, low-grade adenomas (LGAs) and high-grade adenomas (HGAs) was 0%, 9.09% and 81.25%. For APC, immunoreactive score (IRS) was 5.50 ± 0.5 in PTs, 3.59 ± 1.4 in LGAs and 1.8 ± 2.0 in HGAs. The scores in LGAs and HGAs were significantly lower than those in PTs (P = 0.000). IRS reflected significantly reduced expression of APC in HGAs (P = 0.002). The absent expression of APC had a correlation with the expression of ß-catenin (P = 0.000). Four LGAs (18.18%) and nine HGAs (56.25%) had methylation of APC. APC promoter methylation correlated with the grade (P = 0.014) and the expression of ß-catenin and APC (P = 0.000). Genes mutation was detected in only two adenomas (5.3%). The presence of Hp in HGAs (43.8%) was significantly higher than in LGAs (13.6%) (P = 0.038). But there was no statistical correlation to growth pattern, size, APC hypermethylation and gene mutation. CONCLUSION: Hypermethylation of APC promoter, instead of mutations involving APC and ß-catenin, may play a role in the development and progression of GAs contributing to moderate activation of Wnt signaling. Helicobacter pylori may accelerate the progress of gastric adenoma, but the pathogenesis needs further research.


Assuntos
Adenoma/genética , Proteína da Polipose Adenomatosa do Colo/genética , Metilação de DNA , Lesões Pré-Cancerosas/genética , Regiões Promotoras Genéticas , Neoplasias Gástricas/genética , Via de Sinalização Wnt/genética , Adenoma/química , Adenoma/microbiologia , Adenoma/patologia , Proteína da Polipose Adenomatosa do Colo/análise , Transformação Celular Neoplásica/química , Transformação Celular Neoplásica/genética , Análise Mutacional de DNA , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Helicobacter pylori/genética , Helicobacter pylori/isolamento & purificação , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Reação em Cadeia da Polimerase , Lesões Pré-Cancerosas/química , Lesões Pré-Cancerosas/microbiologia , Lesões Pré-Cancerosas/patologia , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/microbiologia , Neoplasias Gástricas/patologia , beta Catenina/análise , beta Catenina/genética
6.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 29(2): 121-5, 2012 Apr.
Artigo em Zh | MEDLINE | ID: mdl-22487816

RESUMO

OBJECTIVE: To screen for potential mutations of LKB1 gene in Chinese familial Peutz-Jeghers syndrome (PJS) patients and analyze their clinical manifestations. METHODS: Eleven PJS families were collected and genomic DNA of peripheral blood was extracted. Typically mucosal pigmentation and hamartomatous polyps were present in all 11 probands. Mutation screening of the probands were carried out by PCR and direct sequencing. Two hundred and fifty healthy adults were enrolled as normal controls, for whom genomic DNA of peripheral blood was also extracted. PCR-denaturing high performance liquid chromatography was carried out to verify the mutation identified in the patients. RESULTS: Nine germline mutations were identified in eight PJS patients, which included 7 point mutations, 1 deletion and 1 insertion. Among these, 4 were considered to be pathogenic, of which 2 were de novel, 4 were considered to be polymorphism, and 1 was uncertain. CONCLUSION: LKB1 gene mutations with pathogenic effect are a common cause of familial PJS in Chinese patients. Most mutations are point mutations.


Assuntos
Mutação em Linhagem Germinativa , Síndrome de Peutz-Jeghers/genética , Proteínas Serina-Treonina Quinases/genética , Quinases Proteína-Quinases Ativadas por AMP , Adolescente , Adulto , Sequência de Bases , China , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Adulto Jovem
7.
Org Lett ; 21(21): 8852-8856, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31642679

RESUMO

A copper-catalyzed efficient enantioselective construction of chiral quaternary carbon-containing chromanes and 3,4-dihydropyrans is reported. The desymmetric C-O coupling is enabled by a chiral dimethylcyclohexane-1,2-diamine ligand and provides the desired products in good yields with high enantioselectivities. This method presents a broad substrate scope and is applicable to diversely substituted aryl bromides and alkenyl bromides. The application is demonstrated by a gram-scale synthesis and derivatization of the products toward valuable building blocks.

8.
Biomed Pharmacother ; 108: 540-546, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30243087

RESUMO

Abnormal phosphorylation of claudins changes the interaction and aggregation of tight junction proteins, affecting the intestinal epithelial barrier. Selective blockade of transient receptor potential vanilloid 4 (TRPV4) alleviated experimental colitis. Whether TRPV4 affects the intestinal epithelial barrier and the relationship to claudin-7 phosphorylation remain unknown. In the present study, we investigated the TRPV4 expression in human colonic tissues and colonic cells. Using the site-directed mutagenesis approach, we also identified the roles of claudin-7 phosphorylation in the epithelial barrier and the relationship between TRPV4 and claudin-7 phosphorylation. Increased TRPV4 expression was found in the colonic mucosa from IBD patients. In colonic cells, the mutation of claudin-7 at position 204 decreased the TRPV4 expression. Mutation of claudin-7 at position 204 significantly decreased the FD20 permeability in monolayer colonic cells, while mutations of claudin-7 at positions S206 and S207 increased the FD20 permeability. Meanwhile, mutations of claudin-7 at positions S204 and S207 increased the TER in monolayer colonic cells. TRPV4 agonist GSK1016790 A increased the FD20 permeability in the control group, cld7-wild group, cld7-S206A group and cld7-S207 A group, while the TRPV4 antagonist HC067047 decreased the FD20 permeability in the same groups. HC067047 treatment increased the TER in vector cells, cld7-wild cells and cld7-S206 A cells compared to the respective cells in GSK1016790A-treated groups. HC067047 treatment decreased the migration in vector cells, cld7-wild cells and cld7-S206 A cells compared to the respective cells in the GSK1016790A-treated groups. These results indicated that TRPV4 might be a target for the maintenance of the intestinal epithelial barrier and indicated the mechanism involved in the modulation of serine phosphorylated claudin-7.


Assuntos
Claudinas/metabolismo , Colo/metabolismo , Células Epiteliais/metabolismo , Canais de Cátion TRPV/metabolismo , Linhagem Celular , Colite/metabolismo , Humanos , Mucosa Intestinal/metabolismo , Permeabilidade , Fosforilação/fisiologia
9.
World J Gastrointest Endosc ; 6(1): 27-31, 2014 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-24527178

RESUMO

Behcet's disease (BD) is a rare and life-long disorder characterized by inflammation of blood vessels throughout the body. BD was originally described in 1937 as a syndrome involving oral and genital ulceration in addition to ocular inflammation. Intestinal BD refers to colonic ulcerative lesions documented by objective measures in patients with BD. Many studies have shown that over 40% of BD patients have gastrointestinal complaints. Symptoms include abdominal pain, diarrhea, nausea, anorexia and abdominal distension. Although gastrointestinal symptoms are common, the demonstration of gastrointestinal ulcers is rare. This so-called intestinal BD accounts for approximately 1% of cases. There is no specific test for BD, and the diagnosis is based on clinical criteria. The manifestations of intestinal BD are similar to those of other colitis conditions such as Crohn's disease or intestinal tuberculosis, thus, it is challenging for gastroenterologists to accurately diagnose intestinal BD in patients with ileo-colonic ulcers. However, giant ulcers distributed in the esophagus and ileocecal junction with gastrointestinal hemorrhage are rare in intestinal BD. Here, we present a case of untypical intestinal BD. The patient had recurrent aphthous ulceration of the oral mucosa, and esophageal and ileo-colonic ulceration, but no typical extra-intestinal symptoms. During examination, the patient had massive acute lower gastrointestinal bleeding. The patient underwent ileostomy after an emergency right hemicolectomy and partial ileectomy, and was subsequently diagnosed with incomplete-type intestinal BD by pathology. The literature on the evaluation and management of this condition is reviewed.

10.
J Dig Dis ; 14(7): 358-65, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23517408

RESUMO

OBJECTIVE: The study aimed to investigate the relationship between gastroduodenal disease and the diversity of the cagA 3' variable region and the amino acid polymorphisms in the Glu-Pro-Ile-Tyr-Ala (EPIYA) segments of the CagA C-terminal region of Helicobacter pylori (H. pylori). METHODS: Gastric mucosal specimens from 170 patients in our center (Nanjing, Jiangsu Province, China) were collected and the genomic DNA of the H. pylori strains was extracted directly from biopsied specimens. Polymerase chain reaction (PCR) was used to amplify the cagA gene, and diversity in its 3' variable region was assessed by direct sequencing. RESULTS: A total of 154 (90.6%) H. pylori isolates were cagA-positive, but the presence of this gene alone was not associated with the type of gastroduodenal disease. A total of 151 (88.8%) strains had the East Asian type EPIYA-D sequence, most of which were of ABD subtype. Three isolates from patients with chronic gastritis possessed the EPIYA-C segment. The sequences flanking the EPIYA motifs contained polymorphisms at seven residues, among which amino acid positions 878 and 879 had a statistically significant association with gastric cancer (P = 0.021). Amino acid change from glycine to aspartic acid at residue 968 was present only in patients with gastric cancer (4/20) (P < 0.001). CONCLUSIONS: Most H. pylori strains present in our study are of the CagA-ABD subtype. Polymorphisms at amino acids 878 and 879 flanking the EPIYA-A motif are statistically associated with gastric cancer.


Assuntos
Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Infecções por Helicobacter/complicações , Helicobacter pylori/genética , Neoplasias Gástricas/microbiologia , Adulto , Motivos de Aminoácidos/genética , Sequência de Aminoácidos , Substituição de Aminoácidos , China/epidemiologia , Doença Crônica , Úlcera Duodenal/epidemiologia , Úlcera Duodenal/microbiologia , Feminino , Gastrite/epidemiologia , Gastrite/microbiologia , Genes Bacterianos , Variação Genética , Infecções por Helicobacter/epidemiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/isolamento & purificação , Helicobacter pylori/patogenicidade , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Polimorfismo Genético , Alinhamento de Sequência , Neoplasias Gástricas/epidemiologia , Úlcera Gástrica/epidemiologia , Úlcera Gástrica/microbiologia , Virulência/genética , Fatores de Virulência/genética
11.
World J Gastroenterol ; 16(12): 1548-52, 2010 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-20333801

RESUMO

Klippel-Trenaunay syndrome is a congenital vascular anomaly characterized by a triad of varicose veins, cutaneous capillary malformation, and hypertrophy of bone and (or) soft tissue. Gastrointestinal vascular malformations in Klippel-Trenaunay syndrome may present with gastrointestinal bleeding. The majority of patients with splenic hemangiomatosis and/or left inferior vena cava are asymptomatic. We herein report a case admitted to the gastroenterology clinic with life-threatening hematochezia and symptomatic iron deficiency anemia. Due to the asymptomatic mild intermittent hematochezia, splenic hemangiomas and left inferior vena cava, the patient did not seek any help for gastrointestinal bleeding until his admittance to our department for evaluation of massive gastrointestinal bleeding. He was referred to angiography because of his serious pathogenetic condition and inefficiency of medical therapy. The method showed that hemostasis was successfully achieved in the hemorrhage site by embolism of corresponding vessels. Further endoscopy revealed vascular malformations starting from the stomach to the descending colon. On the other hand, computed tomography revealed splenic hemangiomas and left inferior vena cava. To the best of our knowledge, this is the first Klippel-Trenaunay syndrome case presenting with gastrointestinal bleeding, splenic hemangiomas and left inferior vena cava. The literature on the evaluation and management of this case is reviewed.


Assuntos
Hemorragia Gastrointestinal/etiologia , Hemangioma/etiologia , Síndrome de Klippel-Trenaunay-Weber/complicações , Neoplasias Esplênicas/etiologia , Veia Cava Inferior/anormalidades , Anemia Ferropriva/etiologia , Endoscopia por Cápsula , Colonoscopia , Embolização Terapêutica , Hemorragia Gastrointestinal/diagnóstico , Hemorragia Gastrointestinal/terapia , Gastroscopia , Hemangioma/diagnóstico , Humanos , Síndrome de Klippel-Trenaunay-Weber/diagnóstico , Masculino , Neoplasias Esplênicas/diagnóstico , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Veia Cava Inferior/diagnóstico por imagem , Adulto Jovem
12.
World J Gastroenterol ; 16(24): 2994-3001, 2010 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-20572302

RESUMO

AIM: To investigate the effect of emodin on expression of claudin-4, claudin-5 and occludin, as well as the alveolar epithelial barrier in rats with pancreatitis induced by sodium taurocholate. METHODS: Experimental pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Emodin was injected via the external jugular vein 3 h after induction of acute pancreatitis. Rats from sham operation group and acute pancreatitis group were injected with normal saline (an equivalent volume as emodin) at the same time point. Samples of lung and serum were obtained 6 h after drug administration. Pulmonary morphology was examined with HE staining. Pulmonary edema was estimated by measuring water content in lung tissue samples. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) level were measured by enzyme-linked immunospecific assay. Serum amylase and pulmonary myeloperoxidase (MPO) activity were detected by spectrophotometry. Alveolar epithelial barrier was assessed by pulmonary dye extravasation. Expression of claudin-4, claudin-5 and occludin in lung tissue samples was examined by immunohistology, quantitative real-time reverse transcription polymerase chain reaction and Western blotting analysis, respectively. RESULTS: Pancreatitis-associated lung injury was characterized by pulmonary edema, leukocyte infiltration, alveolar collapse, and elevated serum amylase level. The pulmonary damage, pulmonary pathological scores, serum amylase and MPO activity, TNF-alpha and IL-6 levels, and wet/dry ratio were decreased in rats after treatment with emodin. Immunostaining of claudin-4, claudin-5 and occludin was detected in lung tissue samples from rats in sham operation group, which was distributed in alveolar epithelium, vascular endothelium, and bronchial epithelium, respectively. The mRNA and protein expression levels of claudin-4, claudin-5 and occludin in lung tissue samples were markedly decreased, the expression level of claudin-4, claudin-5 and occluding was increased, and the pulmonary dye extravasation was reduced in lung tissue samples from rats with acute pancreatitis after treatment with emodin. CONCLUSION: Emodin attenuates pulmonary edema and inflammation, enhances alveolar epithelial barrier function, and promotes expression of claudin-4, claudin-5 and occludin in lung tissue samples from rats with acute pancreatitis.


Assuntos
Barreira Alveolocapilar/efeitos dos fármacos , Emodina , Pancreatite/tratamento farmacológico , Pancreatite/patologia , Alvéolos Pulmonares , Animais , Barreira Alveolocapilar/fisiologia , Claudina-4 , Claudina-5 , Emodina/farmacologia , Emodina/uso terapêutico , Pulmão/anatomia & histologia , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Pulmão/patologia , Masculino , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Ocludina , Pancreatite/induzido quimicamente , Pancreatite/complicações , Inibidores de Proteínas Quinases/farmacologia , Inibidores de Proteínas Quinases/uso terapêutico , Alvéolos Pulmonares/efeitos dos fármacos , Alvéolos Pulmonares/fisiologia , Edema Pulmonar/tratamento farmacológico , Edema Pulmonar/etiologia , Edema Pulmonar/fisiopatologia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Ácido Taurocólico/farmacologia
13.
World J Gastroenterol ; 16(23): 2873-80, 2010 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-20556832

RESUMO

AIM: To investigate the effects of the chemokine stromal cell-derived factor-1 (CXCL12) receptor (CXCR4) antagonist AMD3100 on colonic inflammation and epithelial barrier in dextran sulfate sodium (DSS)-induced colitis in mice. METHODS: Experimental colitis was induced by administration of 5% DSS for 7 d, and assays performed on intestinal segments from the ileocecal valve to the anus. Colonic morphology was examined by hematoxylin and eosin staining. Colonic cytokines were determined by enzyme-linked immunosorbent assay. Myeloperoxidase (MPO) activity (indicator of inflammatory infiltration) was observed spectrophotometrically. Gut permeability was assessed by mucosal-to-serosal clearance of fluorescein isothiocyanate-conjugated dextran 4000 (FD4) in everted gut sacs. The apoptosis of colonic epithelium was assessed by Hoechst-33342 staining. To further elucidate the role of CXCR4 in colonic inflammation, we also investigated the effect of AMD3100 on migration and cytokine production of isolated peripheral blood mononuclear cells (PBMCs). RESULTS: DSS-induced colitis was characterized by morphologic changes, as well as increased colonic cytokines, inflammatory infiltration, epithelial apoptosis, and intestinal permeability in mice. In AMD3100-treated mice, epithelial destruction, inflammatory infiltration, and submucosal edema were markedly reduced; colonic tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interferon-gamma (IFN-gamma) levels, as well as MPO activity were significantly decreased. Increased intestinal permeability in DSS-treated mice was significantly reduced by AMD3100. The number of apoptotic cells in colitis mice was markedly increased after DSS administration, and decreased when treated with the CXCR4 antagonist AMD3100. In pre-activated PBMCs, CXCL12 stimulation significantly increased the migration of PBMCs, and was inhibited by AMD3100. Moderately increased TNF-alpha, IL-6, and IFN-gamma from CXCL12-treated PBMCs were also reduced by AMD3100. CONCLUSION: The CXCR4 antagonist AMD3100 exerts therapeutic effects on experimental colitis by inhibiting colonic inflammation and enhancing epithelial barrier integrity.


Assuntos
Colite/tratamento farmacológico , Compostos Heterocíclicos/farmacologia , Receptores CXCR4/antagonistas & inibidores , Animais , Apoptose/efeitos dos fármacos , Benzilaminas , Movimento Celular/efeitos dos fármacos , Colite/induzido quimicamente , Colite/patologia , Colite/fisiopatologia , Colo/efeitos dos fármacos , Colo/patologia , Colo/fisiopatologia , Ciclamos , Citocinas/metabolismo , Sulfato de Dextrana/toxicidade , Feminino , Técnicas In Vitro , Mediadores da Inflamação/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Permeabilidade/efeitos dos fármacos , Peroxidase/metabolismo
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