RESUMO
Liver cancer results in a high degree of mortality, especially among men. As fatty liver disease is a risk factor for development of hepatocellular carcinoma, we investigated the role of dietary fat type in tumor promotion by high-fat diets in mice after initiation with the chemical carcinogen diethyl nitrosamine. Tumor incidence and multiplicity were significantly greater in males than those in females. In males, fat type had complex effects on tumorigenesis. Preneoplastic foci were most prevalent in mice fed a polyunsaturated fat diet enriched in docosahexaenoic acid, whereas carcinomas and large visible liver tumors were significantly greater in mice fed a saturated fat diet made with cocoa butter relative to mice fed mono- or polyunsaturated fats. Different mechanisms thus seemed involved in early and late tumor promotion. The hepatic transcriptome and gut microbiome were assessed for traits associated with tumorigenesis. Hepatic expression of more than 20% of all genes was affected by sex, whereas fat type affected fewer genes. In males, the saturated fat diet induced expression of the proto-oncogene Agap2 and affected the expression of several cytochrome P450 genes, and genes involved in lipid, bile acid and fatty acid metabolism. The gut microbiome had a higher level of genus Akkermansia and a lower level of Firmicutes in females than in males. Males fed saturated fat had an altered microbiome, including an enrichment of the genus Coprococcus. In conclusion, sex and the dietary fat type affect the gut microbiome, the hepatic transcriptome and ultimately hepatic tumor growth.
Assuntos
Carcinogênese/patologia , Dieta Hiperlipídica/efeitos adversos , Proteínas de Ligação ao GTP/metabolismo , Microbioma Gastrointestinal/fisiologia , Neoplasias Hepáticas/etiologia , Proto-Oncogenes/fisiologia , Animais , Ácidos e Sais Biliares/metabolismo , Carcinogênese/metabolismo , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/microbiologia , Carcinoma Hepatocelular/patologia , Gorduras na Dieta/efeitos adversos , Ácidos Docosa-Hexaenoicos/farmacologia , Ácidos Graxos/metabolismo , Feminino , Metabolismo dos Lipídeos/fisiologia , Fígado/metabolismo , Fígado/microbiologia , Fígado/patologia , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/microbiologia , Neoplasias Hepáticas/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BLRESUMO
Red-bellied lemurs (Eulemur rubriventer: genus Eulemur, family Lemuridae, Suborder Strepsirrhini) are non-human primates endemic to the forests of Madagascar and listed as 'vulnerable' on the IUCN Red List of Threatened Species. Currently, descriptions of neoplasia in this species are extremely scarce, with only one case of hepatocellular adenoma reported. Prosimian submissions received by the Anatomic Pathology Service at North Carolina State University from January 2010 to January 2021 were retrieved. A total of 200 cases of Strepsirrhini prosimians were identified, representing 57 (28.5%) individuals from the genus Eulemur of which seven (12.3%) cases were red-bellied lemurs. Neoplasia was identified in two of the seven (28.57%) cases. The first case was a 25-year-old, intact female with hepatocellular carcinoma and the second a 33-year-old, intact female with a cervical mass consistent with osteoblastic osteosarcoma arising from the left clavicular bone. Hepatocellular neoplasms are commonly reported in prosimians, with carcinomas more frequent than adenomas. In contrast, osteosarcoma has been rarely described in lemuriformes, with four cases reported. To our knowledge, this is the first report of osteosarcoma and hepatocellular carcinoma in a red-bellied lemur.
Assuntos
Neoplasias Ósseas , Carcinoma Hepatocelular , Lemur , Lemuridae , Neoplasias Hepáticas , Osteossarcoma , Strepsirhini , Feminino , Animais , Carcinoma Hepatocelular/veterinária , Osteossarcoma/veterinária , Neoplasias Hepáticas/veterinária , Neoplasias Ósseas/veterináriaRESUMO
In the present systematic review and meta-analysis, we evaluated the effects of providing piglets with creep feed during lactation on piglet pre- and post-weaning performance. A total of 20 articles met the inclusion criteria. Creep feeding in lactation improved pre-weaning piglet performance in 46% of the studies selected, while 58% of the included studies reported that creep feeding in lactation improved piglet performance during the nursery phase. Creep feeding increased the average piglet body weight (creep = 7.23 ± 0.30, no creep = 6.96 ± 0.31; p = 0.03) and litter weight (creep = 81.2 ± 4.18, no creep = 76.4 ± 4.22; p < 0.001) at weaning. The average piglet body weight and litter weight were positively associated (p < 0.001 and p < 0.001, respectively) with total creep feed intake. Creep feeding of piglets for more than 14 days increased (p = 0.003) the litter weight at weaning compared to litters not provided or provided for shorter periods with creep feed. The present work strengthened the notion that creep feeding during lactation presents opportunities for improving weaning weights and post-weaning piglet performance compared to litters not provided or provided for shorter periods with creep feed.
RESUMO
In collaboration with the American College of Veterinary Pathologists.
Assuntos
Patologia Veterinária , Médicos Veterinários , Animais , Humanos , Estados UnidosRESUMO
In collaboration with the American College of Veterinary Pathologists.
Assuntos
Patologia Veterinária , Médicos Veterinários , Animais , Humanos , Estados UnidosRESUMO
In collaboration with the American College of Veterinary Pathologists.
Assuntos
Patologia Veterinária , Médicos Veterinários , Animais , Humanos , Estados UnidosRESUMO
A 9-year-old intact male Brazilian Mastiff dog with a 1-year history of progressive severe swelling in all four limbs and significant locomotory difficulty was euthanized due to unresponsiveness to medical management. Macroscopically, the distal phalanx of the 5th digit of the left hindlimb was replaced by a cutaneous, non-ulcerated, 3.0 cm diameter, multilobulated, black, firm nodule. The cortical bones of the appendicular skeleton were thickened and partially effaced by infiltrative coalescing nodules (0.1-1.0 cm diameter). The lungs, heart, pleura, mesentery, adrenal glands and kidneys were infiltrated by similar nodules (0.5-3.5 cm diameter). Histological evaluation of the masses revealed a non-encapsulated malignant neoplasm composed of spindloid to polygonal cells that contained variable amounts of intracytoplasmic melanin and were arranged in haphazardly interlacing streams and bundles supported by scant fibrovascular stroma with myxoid and chondroid matrix formation. Neoplastic cells had intense cytoplasmic labeling for S100 and 10% had moderate cytoplasmic immunoreactivity for Melan-A. To the authors' knowledge, this is the first description of a canine metastatic digital chondrogenic melanocytic tumour with strongly suggestive chondroid differentiation of neoplastic melanocytes.
Assuntos
Neoplasias Ósseas , Doenças do Cão , Melanoma , Neoplasias Cutâneas , Animais , Neoplasias Ósseas/veterinária , Brasil , Cães , Masculino , Melanócitos , Melanoma/veterinária , Neoplasias Cutâneas/veterináriaRESUMO
An adult alpaca (Vicugna pacos) with a history of colic and anorexia was euthanized because of failure to respond to treatment. Macroscopically, pale-tan, multifocal to coalescing, firm nodules and plaques markedly expanded the omentum, mesentery and the parietal and visceral peritoneum of multiple abdominal organs, especially the right oviduct and associated mesosalpinx. Abundant dark-red watery digesta were present in the duodenum and jejunum. Histological evaluation of the right oviduct, abdominal visceral nodules and plaques and mesenteric lymph nodes revealed transmural expansion and replacement by an epithelial malignant neoplasm, comprised of tubules and acini of ciliated columnar cells supported by abundant fibrous connective tissue. Both ovaries were histologically normal. On the basis of the ciliated morphology of the neoplastic cells, the focus on the proximal reproductive tract and the unremarkable ovaries, a reproductive tubal adenocarcinoma with carcinomatosis was diagnosed, with both the endometrium and oviduct considered as the tissues of origin. The prominent ciliated morphology of the neoplastic cells and the classification of human fallopian tube (oviduct) neoplasia lead us to propose oviductal adenocarcinoma with widespread carcinomatosis as the definitive diagnosis. The lamina propria of the small intestine was infiltrated segmentally by lymphocytes, plasma cells and neutrophils, and Clostridium perfringens with beta2 toxin production was identified by polymerase chain reaction in the small intestinal contents. To our knowledge, this is the first report of these two distinct diseases in an alpaca.
Assuntos
Adenocarcinoma , Camelídeos Americanos , Enterite , Neoplasias Peritoneais , Adenocarcinoma/veterinária , Animais , Enterite/veterinária , Feminino , Neoplasias Peritoneais/veterinária , Reação em Cadeia da Polimerase/veterináriaRESUMO
This report describes the pathologic findings in a single, adult female Cuvier's beaked whale Ziphius cavirostris stranded in the Canary Islands. The study indicated that this whale died with a severe, systemic, herpesviral infection and clearly exhibited lesions different from those of the fat and gas embolic syndrome described in beaked whale mass strandings associated with sonar exposure. This is the first report of a cetacean alphaherpesvirus infection of the lymphoid system in a beaked whale.
Assuntos
Alphaherpesvirinae/isolamento & purificação , Infecções por Herpesviridae/veterinária , Linfonodos/patologia , Necrose/veterinária , Baleias , Alphaherpesvirinae/classificação , Alphaherpesvirinae/genética , Animais , Feminino , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/patologia , Linfonodos/virologia , Linfadenite/patologia , Linfadenite/veterinária , Linfadenite/virologia , Necrose/patologia , Necrose/virologia , Filogenia , EspanhaRESUMO
Polycystic liver is usually considered an incidental finding in human and veterinary medicine. Two unrelated adult llamas ( Lama glama) with a history of marked anorexia and weight loss were received for autopsy and diagnostic workup. The main gross change in the liver of both animals was multiple variably sized cysts randomly distributed throughout the parenchyma. Histologically, the cysts compressed the adjacent parenchyma and were lined by a single layer of cuboidal-to-columnar epithelium, surrounded by a fibrous collagen capsule. The lumen of the cysts contained finely granular-to-homogeneous basophilic material. The lining epithelium displayed strong immunoreactivity for pancytokeratin AE1/AE3 and cytokeratins 7, 8, 8/18, and 19, and was negative for vimentin, confirming the biliary epithelial origin of the cysts. No parasitic or infectious agents, or neoplastic changes, were detected. All other laboratory tests performed in both llamas were negative or non-diagnostic, suggesting that the congenital hepatic cysts described may have been at least partly responsible for clinical disease in both animals.
Assuntos
Camelídeos Americanos , Cistos/veterinária , Hepatopatias/veterinária , Animais , Cistos/congênito , Cistos/diagnóstico , Cistos/patologia , Feminino , Hepatopatias/congênito , Hepatopatias/diagnóstico , Hepatopatias/patologia , MasculinoRESUMO
Rotavirus (RV) is an important viral pathogen causing diarrhea in piglets and other mammals worldwide. We describe 34 cases from 4 diarrheal outbreaks caused by RV in unvaccinated farrowing units in southern Brazil from 2011 to 2013. We performed autopsy, histologic examinations, bacterial culture, RV immunohistochemistry (IHC), and enteric virus detection through molecular assays for rotavirus A, B, and C, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, sapovirus, norovirus, and kobuvirus. Histologically, villus atrophy (29 of 34) and epithelial vacuolation (27 of 34) occurred in all 4 outbreaks. Cell debris in the lamina propria occurred in 20 cases, mostly from outbreaks A (8 of 11), C (4 of 6), and D (7 of 11). IHC was positive for RV in 21 of 34 samples. RT-PCR was positive for RV in 20 of 30 samples; RV-C was the most frequently detected RV ( n = 17). Kobuvirus was detected in 11 samples, and, in 3 of them, there was single detection of this enteric virus.
Assuntos
Diarreia/veterinária , Surtos de Doenças/veterinária , Infecções por Rotavirus/veterinária , Rotavirus/genética , Doenças dos Suínos/epidemiologia , Animais , Animais Lactentes , Brasil/epidemiologia , Diarreia/epidemiologia , Fezes/virologia , Imuno-Histoquímica/veterinária , Filogenia , Rotavirus/isolamento & purificação , Infecções por Rotavirus/epidemiologia , Suínos , Doenças dos Suínos/diagnóstico , Doenças dos Suínos/virologiaRESUMO
We describe 3 outbreaks of superficial dermatitis caused by bovine herpesvirus 2 (BoHV-2) in dairy breed calves. Clinically, all of the affected calves were 12-26 d of age, had alopecia and crusts on the face and ears, and were non-pruritic and afebrile. Affected animals recovered spontaneously without any treatment within 2-4 wk after onset of clinical signs based on 1 herd with follow up. Histologic examination of all skin crust or tissue samples identified neutrophilic inflammation, mild hyperkeratosis, multinucleate syncytial cells, and intranuclear inclusion bodies in the syncytial cells. Real-time PCR testing on affected surface crusts or tissue provided evidence of BoHV-2, and testing, where performed, was negative for parapoxvirus including bovine papular stomatitis virus and the ovine form of malignant catarrhal fever tested in EDTA blood samples. Bovine viral diarrhea virus also was negative by ELISA, as well as bovine herpesvirus 1 by immunohistochemistry. Direct electron microscopy of infected tissues in the first outbreak revealed herpesvirus-like particles.
Assuntos
Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Herpes Simples/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Animais , California/epidemiologia , Bovinos , Doenças dos Bovinos/virologia , Orelha/patologia , Feminino , Cabeça/patologia , Herpes Simples/diagnóstico , Herpes Simples/epidemiologia , Herpes Simples/virologia , Pele/patologiaRESUMO
Samples of the liver, telencephalon, brainstem, and cerebellum were obtained from 22 bovids suffering from spontaneous or experimental acute toxic liver disease. Perreyia flavipes larvae, and leaves of Cestrum corymbosum, Cestrum intermedium, Dodonaea viscosa, Trema micrantha, and Xanthium cavanillesii were the causal agents in the disorders studied. Hematoxylin and eosin and periodic acid-Schiff staining, as well as anti-S100 protein (anti-S100), anti-glial fibrillary acidic protein (anti-GFAP), and anti-vimentin immunostaining were used to evaluate the brain sections. Astrocytic changes were observed in all samples and were characterized by swollen vesicular nuclei in gray (Alzheimer type II astrocytes) and white matter; and by abundant eosinophilic or vacuolated cytoplasm with pyknotic nuclei in the white matter. These changes were evidenced by anti-S100 and anti-GFAP immunostaining. Our study demonstrates major changes in astrocytes of cattle that died with neurologic clinical signs as the result of acute toxic liver disease.
Assuntos
Doenças dos Bovinos/diagnóstico , Doença Hepática Induzida por Substâncias e Drogas/veterinária , Animais , Astrócitos/citologia , Encéfalo/metabolismo , Bovinos , Doenças dos Bovinos/metabolismo , Doenças dos Bovinos/patologia , Cerebelo/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/diagnóstico , Proteína Glial Fibrilar Ácida/metabolismo , Imuno-Histoquímica/veterinária , Fígado/metabolismo , Coloração e RotulagemRESUMO
Infection by Rhodococcus equi is considered one of the major health concerns for foals worldwide. In order to better understand the disease's clinical and pathological features, we studied twenty cases of natural infection by R. equi in foals. These cases are characterized according to their clinical and pathological findings and immunohistochemical aspects. Necropsy, histologic examination, bacterial culture, R. equi and Pneumocystis spp. immunohistochemistry were performed. The foals had a mean age of 60 days and presented respiratory signs (11/20), hyperthermia (10/20), articular swelling (6/20), prostration (4/20), locomotor impairment (3/20) and diarrhea (3/20), among others. The main lesions were of pyogranulomatous pneumonia, seen in 19 foals, accompanied or not by pyogranulomatous lymphadenitis (10/20) and pyogranulomatous and ulcerative enterocolitis (5/20). Pyogranulomatous osteomyelitis was seen in 3 foals, one of which did not have pulmonary involvement. There was lymphoplasmacytic (4/20), lymphoplasmacytic and neutrophilic (1/20) or pyogranulomatous arthritis (1/20), affecting multiple or singular joints. Immunohistochemistry revealed to be a valuable tool for the detection of R. equi, confirming the diagnosis in all cases. Furthermore, pulmonary immunostaining for Pneumocystis spp. demonstrates that a coinfection with R. equi and this fungal agent is a common event in foals, seen in 13 cases.(AU)
Infecção por Rhodococcus equi é considerado um dos maiores problemas sanitários para potros em todo o mundo. Para melhor compreender a apresentação clínica e patológica da enfermidade, foram avaliados vinte casos de infecção natural por R. equi em potros. Os casos são caracterizados de acordo com seus achados clínicos e patológicos e aspectos imuno-histoquímicos. Foram realizados exames de necropsia, histologia, bacteriologia e imuno-histoquímica para R. equi e Pneumocystis spp. Os potros tinham idade media de 60 dias e apresentaram sinais respiratórios (11/20), hipertermia (10/20), aumento de volume articular (6/20), prostração (4/20), distúrbios locomotores (3/20) e diarreia (3/20), entre outros. As lesões mais importantes eram pneumonia piogranulomatosa, vista em 19 potros, acompanhada ou não por linfadenite piogranulomatosa (10/20) e enterocolite ulcerativa (5/20). Osteomielite piogranulomatosa foi constatada em três potros, um dos quais não apresentava envolvimento pulmonar. Artrites afetando uma ou múltiplas articulações eram caracterizadas por infiltrado linfoplasmocítico (4/20), linfoplasmocítico e neutrofílico (1/20) e piogranulomatoso (1/20). A imuno-histoquímica demonstrou ser uma ferramenta valiosa na detecção de R. equi, permitindo confirmar o diagnóstico em todos os casos avaliados. Além disso, a imuno-histoquímica para Pneumocystis spp. demonstra que a coinfecção por R. equi e o agente fúngico é um evento frequente em potros, constatado em 13 casos.(AU)
Assuntos
Animais , Pneumonia por Pneumocystis/veterinária , Pneumonia por Pneumocystis/epidemiologia , Infecções por Actinomycetales/veterinária , Infecções por Actinomycetales/epidemiologia , Rhodococcus equi/isolamento & purificação , Doenças dos Cavalos/microbiologia , CavalosAssuntos
Adenocarcinoma Mucinoso/veterinária , Neoplasias do Colo/veterinária , Sus scrofa , Doenças dos Suínos/diagnóstico , Adenocarcinoma Mucinoso/diagnóstico , Adenocarcinoma Mucinoso/patologia , Animais , Colo/patologia , Neoplasias do Colo/diagnóstico , Neoplasias do Colo/patologia , Eutanásia Animal , Evolução Fatal , Feminino , Mucosa Intestinal/patologia , Obstrução Intestinal/diagnóstico , Obstrução Intestinal/etiologia , Obstrução Intestinal/patologia , Obstrução Intestinal/veterinária , Ovariectomia , Prognóstico , Suínos , Doenças dos Suínos/patologiaRESUMO
There is a current need for new therapeutic options for acetaminophen (APAP)-induced hepatotoxicity. Herein, we assessed the effects of prophylactic and therapeutic treatment with the angiotensin-converting enzyme (ACE) inhibitor, enalapril, on APAP-caused hepatotoxicity. Male and female C57BL/6 J mice were used, and hepatotoxicity was induced by a single application of APAP (400 mg/kg, i.p.). Macroscopic and histological liver alterations, serum alanine transaminase (ALT) and aspartate transaminase (AST) activity, liver catalase activity (CAT), reduced glutathione concentrations (GSH), hepatic measurement of neutrophil migration (myeloperoxidase, MPO activity), and caspase-3 liver expression were evaluated. The prophylactic and the therapeutic treatments with enalapril were able to markedly reduce the macroscopic and histological liver alterations as well as the caspase-3 immunopositivity. Both schedules of treatment were also effective in reducing GSH concentrations as well as neutrophil migration. Conversely, only the pre-treatment (but not the post-administration) with enalapril significantly reversed APAP-induced CAT decrease. Furthermore, the pre- or the post-treatment with enalapril largely reduced ALT and AST serum activity in APAP-intoxicated mice. The hepatoprotective effects of enalapril were comparable to those obtained with the clinically used compound N-acetylcysteine (NAC) when given in a therapeutic regimen. Data obtained with the prophylactic protocol of treatment might indicate that individuals under treatment with ACE inhibitors are less susceptible to the toxic effects of APAP. Additionally, the therapeutic approach allows us to suggest that enalapril might represent an innovative tool for treating APAP intoxication.
Assuntos
Acetaminofen/toxicidade , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Enalapril/farmacologia , Acetilcisteína/farmacologia , Alanina Transaminase/sangue , Analgésicos não Narcóticos/toxicidade , Animais , Aspartato Aminotransferases/sangue , Catalase/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Doença Hepática Induzida por Substâncias e Drogas/patologia , Feminino , Glutationa/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BLRESUMO
In this retrospective study was determined the frequency of canine skin peripheral nerve sheath tumors (PNST) in cases diagnosed by the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brazil, between the years 2000 and 2012. The canine profiles, as well as histological, immunohistochemical and prognostic aspects of the tumors were based on 70 samples, comprising 40 females, 29 males and one unspecified sample. Between 2000 and 2012, 2,984 skin tumors of dogs were diagnosed in the SPV-UFRGS, totaling 2.34% of skin neoplasms in dogs. Animals that comprised the largest amount of samples (43%) were those with no breed (SRD), followed by German Shepherds (10%). Females were more affected than males (40/70 - 57% and 29/70 - 41% respectively). Skin PNST of this research showed predominant localization on the limbs (40% in the forelimbs and 29% in the hindlimbs); affecting adult dogs, mostly aged between 8 and 11 years (54%). The samples were routinely processed for hematoxylin and eosin, and were also evaluated by toluidine blue and Masson's trichrome staining, and immunohistochemistry (IHC) anti-vimentin, -S-100, -GFAP, -actin, von Willebrand factor and neurofilament. Anisocytosis and anisokaryosis, mitotic index, intratumoral necrosis, invasion of adjacent tissues, tumor location, local recurrence and metastasis were related to the diagnosis of benign (49/70) or malignant tumor (21/70). The Antoni A histological pattern was observed more frequently in benign tumors. The immunohistochemistry helped to diagnose PNST, and anti-vimentin and anti-protein S-100 showed the highest rates of immunostaining. Throughout statistical analysis of animals with tumor recurrence, it was found that the chance of an animal with a malignant peripheral nerve sheath tumor to develop recurrence is 4.61 times higher than in an animal that had a benign tumor...
Neste estudo retrospectivo foi determinada a frequência dos tumores de bainha de nervo periférico (TBNP) na pele de cães diagnosticados pelo Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brasil, no período de 2000 a 2012. O histórico dos cães, assim como a histologia, a imuno-histoquímica e o prognóstico em relação aos tumores foram baseados em 70 amostras histológicas, correspondendo a 40 fêmeas (57%), 29 machos (41%) e uma amostra sem a informação do sexo. De 2000 a 2012, foram diagnosticados 2984 tumores de pele de cães pelo SPV-UFRGS, totalizando 2,34% das neoplasias em cães. A maior parte das amostras (43%) foi proveniente de animais sem raça definida (SRD), seguidos pelos da raça Pastor Alemão (10%). A localização predominante dos TBNP desta pesquisa ocorreu em membros (40% em membro tóracico e 29% em membro pélvico), afetando principalmente cães de oito a 11 anos idade (54%). As amostras foram processadas rotineiramente para coloração de Hematoxilina e eosina, e foram realizadas as colorações de azul de toluidina e tricrômio de Masson, assim como realizada imuno-histoquímica (IHQ) anti-vimentina, -S-100, -GFAP, -actina, von Willbrand e neurofilamento. Os fatores anisocitose e anisocariose, índice mitótico, necrose intratumoral, invasão tumoral em tecidos adjacentes, localização tumoral, local de recorrência e metástase foram relacionados com o diagnóstico de benignidade (49/70) ou malignidade (21/70). O padrão histológico de Antoni A foi observado com mais frequência em tumores benignos. A IHQ auxiliou no diagnóstico dos TBNP, com maiores índices de imunomarcação anti-vimentina e S-100. A análise estatística das amostras de animais que apresentaram recidiva tumoral evidenciou que a chance de um animal com tumor de bainha de nervo periférico maligno desenvolver recidiva é 4,61 vezes maior do que em animais que apresentam tumor benigno...
Assuntos
Animais , Cães , Neoplasias de Bainha Neural/veterinária , Neurilemoma/veterinária , Coloração e Rotulagem/veterinária , Imuno-Histoquímica/veterinária , Neoplasias Cutâneas/veterinária , Técnicas Histológicas/classificação , Técnicas Histológicas/veterináriaRESUMO
A clamidiose é causada por Chlamydophila psittaci e representa uma das principais zoonoses de origem aviária. Realizou-se um estudo retrospectivo em psitacídeos do período de 1995 a 2012 e exame imuno-histoquímico (IHQ) anti-Chlamydia. Foram avaliados 111 casos, dos quais 12 foram a óbito devido à clamidiose. As aves eram provenientes de apreensão ou cativeiro (zoológicos, criatórios, centros de triagem e domicílios). À necropsia observou-se fígado aumentado (4/12) com áreas branco-amareladas (3/12), baço aumentado (2/12) e rompido (1/12), saco pericárdico com deposição de fibrina (1/12), polisserosite fibrinosa (1/12) e em três casos não havia lesões. Na avaliação histopatológica evidenciou-se hepatite necrótica mononuclear (7/12), hepatite mononuclear (3/12), hiperplasia de ductos biliares (8/12), esplenite necrótica histiocitária (9/12), hemossiderose em fígado (9/12) e baço (9/12), aerossaculite mononuclear (4/12), pericardite fibrino-heterofílica (2/12), necrose (1/12) e rarefação (1/12) linfoide de bursa de Fabricius, pneumonia fibrinosa (1/12), nefrite mononuclear (1/12) e granulomas renais (1/12). Observaram-se inclusões basofílicas intracitoplasmáticas (corpos elementares) em fígado (2/12), baço e rins (1/12). Evidenciou-se imunomarcação anti-Chlamydia em fígado (11/12), baço (7/9), pulmões (3/9), rins (2/8), intestinos (2/3), sacos aéreos (1/4) e bursa de Fabricius (1/2). A IHQ poderá ser utilizada como forma de diagnóstico definitivo post mortem de clamidiose em psitacídeos no Brasil...
Chlamydiosis is caused by Chlamydophila psittaci and is one of the most important avian zoonosis. A retrospective study in psittacines was performed from 1995 to 2012 with immunohistochemistry (IHC) anti-Chlamydia. Hundred eleven cases were evaluated and twelve birds died due to chlamydiosis. The birds were obtained from illegal commerce traffic or captive conditions (zoos, breeding birds, wildlife rehabilitation center and pets). Grossly, there were hepatomegaly (4/12) with yellowish-white areas (3/12), splenomegaly (2/12), splenic rupture (1/12), fibrin deposition in pericardial sac (1/12), fibrinous polyserositis (1/12), and in three cases lesion was not found. Histopathological evaluation revealed mononuclear necrotizing hepatitis (7/12), mononuclear hepatitis (3/12), biliary duct hyperplasia (8/12), histiocytic necrotizing splenitis (9/12), hemosiderosis in liver (9/12) and spleen (9/12), mononuclear aerosaculitis (4/12), fibrin heterophilic pericarditis (2/12), lymphoid necrosis (1/12) and depletion of bursa Fabricius (1/12), fibrinous pneumonia (1/12), mononuclear nephritis (1/12), and renal granulomas (1/12). Basophilic intracytoplasmic inclusions (elementary bodies) were observed in liver (2/12), spleen and kidney (1/12). Positive immunostaining for Chlamydia could be detected in liver (11/12), spleen (7/9), lung (3/9), kidney (2/8), intestines (2/3), air sacs (1/4) and bursa of Fabricius (1/2). It was concluded that IHC can be used as postmortem definitive diagnosis of chlamydiosis in psittacines...
Assuntos
Animais , Chlamydophila psittaci/isolamento & purificação , Imuno-Histoquímica/veterinária , Papagaios/microbiologia , Zoonoses/transmissão , Autopsia/veterinária , DiagnósticoRESUMO
Actinobacilose é uma doença infecciosa, não contagiosa, geralmente crônica, caracterizada por reação inflamatória piogranulomatosa que ocorre em bovinos e, menos comumente, em ovinos, suínos e equinos. Tecidos moles de cabeça, pescoço e linfonodos regionais são afetados. Neste estudo foram compilados e avaliados 18 casos de bovinos dos livros de registro de janeiro de 1997 a maio de 2011 com diagnóstico de actinobacilose. As lesões foram caracterizadas histologicamente, avaliadas por técnicas de histoquímica e imuno-histoquímica. No exame histológico foram evidenciados piogranulomas típicos de actinobacilose contendo clavas eosinofílicas radiadas, circundadas por neutrófilos, macrófagos, linfócitos e abundante tecido conjuntivo fibroso. Todas as lesões apresentaram cocobacilos Gram negativos na coloração de Gram histológico. Abundantes macrófagos tiveram marcação positiva para CD68, especialmente células epitelioides e células gigantes multinucleadas. Foi observada marcação positiva para CD3 (Linfócitos T) em cerca de 1:3 das células do infiltrado inflamatório linfocitário, e marcação positiva para CD79αcy (Linfócitos B) em 2:3 dos linfócitos, indicando que estes correspondem à maioria dos linfócitos nas áreas inflamatórias das lesões de actinobacilose.
Actinobacillosis is a not contagious infectious disease, usually chronic, and characterized by pyogranulomatous inflammatory reaction that occurs in cattle and is less common in sheep, pig, and horse. Soft tissues of head, mouth, and regional lymph nodes are affected. In this retrospective study, lesions suggestive of Actinobacillosis from 18 cases of anatomopathologic bovine samples were retrieved from the archives of Setor de Patologia Veterinária of Universidade Federal do Rio Grande do Sul, from January 1997 to May 2011. The lesions were classified histologically, evaluated by histochemistry, and immunohistochemistry (IHC) in order to standardize inflammatory infiltration. The most frequent histological findings were typical pyogranulomas of actinobacillosis contained radiating eosinophilic clubs surrounded by neutrophils, macrophages, lymphocytes and abundant fibrous connective tissue. Gram-negative coccobacillus bacteria were shown in all lesions by Gram stain. Abundant macrophages were immunopositive for CD68, especially epithelioid cells and multinucleated giant cells. In addition, a positive immunostaining for CD3 (T lymphocyte) was observed in proportion of 1:3 in lymphocyte inflammatory infiltration, while IHC for CD79αcy (B lymphocytes) was obtained in proportion of 2:3. These results indicated that B lymphocytes are the majority of lymphocyte in the inflammatory area.