RESUMO
A monoclonal antibody (ES3A) was raised against a mouse graft-versus-host reaction (GVHR) model. This antibody was against basal cell cytoplasm and reacted with an acidic (pI 6.2) 50 kDa keratin of human epidermis. However, ES3A reacted with several lower layers of epidermal cells in psoriasis and seborrhoeic keratosis. Acanthotic seborrhoeic keratosis showed varying patterns even in a single lesion. If combined with FACS analysis, ES3A-positive cells could be quantified. Normal skin showed 28%, while psoriasis and seborrhoeic keratosis showed 44% and 51%, respectively. ES3A-positive compartments of the acanthotic type of seborrhoeic keratosis were larger than those of the hyperkeratotic type. ES3A may be suitable for quantification of germinative or proliferative cells.
Assuntos
Anticorpos Monoclonais , Dermatite Seborreica/imunologia , Queratinas/imunologia , Ceratose/imunologia , Psoríase/imunologia , Animais , Fusão Celular , Separação Celular , Modelos Animais de Doenças , Células Epidérmicas , Citometria de Fluxo , Imunofluorescência , Reação Enxerto-Hospedeiro/imunologia , Humanos , Immunoblotting , Técnicas In Vitro , Queratinas/análise , Camundongos , PropídioAssuntos
Neoplasias Cutâneas , Adulto , Feminino , Humanos , Lesões Pré-Cancerosas , Neoplasias Cutâneas/patologiaRESUMO
BACKGROUND: An epidemic of cercarial dermatitis caused by Schistosoma spindale cercaria occurred in November 1988 in a district called Chaiya, Surajthani province, in Southern Thailand. CASE REPORTS: Fifty-eight Thai farmers in Chaiya, Surajthani gave a history of itch following immersion in water while planting rice. The area of rice field was flooded for 10 days before farmers started working. The duration of the disease ranged from 2 days to 1 month. Forty-one patients had the disease for the first time, seventeen had a history of previous manifestations. CONCLUSIONS: This epidemic was caused by S. spindale cercaria, which developed in the Indoplanorbis exustus snail, associated with flooding.
Assuntos
Surtos de Doenças , Esquistossomose/epidemiologia , Dermatopatias Parasitárias/epidemiologia , Adulto , Idoso , Animais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Schistosoma/crescimento & desenvolvimento , Schistosoma/isolamento & purificação , Esquistossomose/diagnóstico , Esquistossomose/parasitologia , Dermatopatias Parasitárias/diagnóstico , Dermatopatias Parasitárias/parasitologia , Tailândia/epidemiologiaRESUMO
Using 2-color fluorescein-activated cytometric analysis, HLA-DR antigen expression on peripheral blood T cell subsets was studied in patients with herpes zoster (HZ), pityriasis rosea (PR) and psoriasis. In HZ and PR, HLA-DR was found to be significantly expressed on T cell surfaces (CD3+ cells), when compared to that of the normal control (HZ: p less than 0.001, PR: p less than 0.05). Among T cell subsets, such HLA-DR antigen was predominantly expressed on suppressor/cytotoxic cells (CD8+) in HZ (vs. normal control, p less than 0.01). However, in the case of PR, it was predominantly expressed on helper cells (CD4+; vs. control, p less than 0.05). On the other hand, activated T cell antigen (CD25+) was not significantly expressed on T cells (CD3+) in either HZ or PR. In the T cell subsets, HLA-DR antigen expression returned to normal levels during the recovery phases of HZ and PR.