[Quantitative Analysis of Pinellia ternata in Banxia Decoction by PCR].

Objective: To establish the quantitative detection method for Pinellia ternata in Banxia decoction by polymerase chain reaction( PCR) and to explore a suitable quantitative analysis method for medicinal materials in liquid preparation of traditional Chinese medicine. Methods: The PCR method was adopted for analyzing Pinellia ternata in Banxia decoction based on the specific detection of ITS region. The PCR amplification products were analyzed with agarose gel electrophoresis. The relationship between the percentage of Pinellia ternata in Banxia decoction with the PCR products were established. In addition,Real-Time PCR assay and agarose gel electrophoresis were applied in the quantitative detection. Results: Accurate quantitative analysis of Pinellia ternata medicinal materials can be achieved by two methods,and a better linear correlation in the standard curve( r = 0. 9943) were obtained with agarose gel electrophoresis method. Conclusion: A high specificity and sufficient reproducibility PCR technology with agarose gel eletroghoresis method has been established for the quantitative detection of Pinellia ternata in Banxia decoction. The quantitative detection of medicinal materials in decoction based on PCR technology was reported for the first time.

[The role of white gene and its mechanism during the formation of curly wing in Drosophila melanogaster.] / é»‘è ¹æžœè‡whiteåŸºå› åœ¨H2O2å½±å“ä¸‹å·ç¿ å½¢æˆä¸­çš„ä½œç”¨åŠæœºç†.

Curly wing is one of the most frequently used genetic markers in Drosophila melanogaster, but its molecular mechanism is remained unclear. Previous results have showed that physicochemical stimulation would affect the formation of the cruly wing. Our recent study found that H2O2 could not only affect the eclosion rate of D. melanogaster, but also induce the formation of curly wing. Here, we aimed to uncover the specific factors influencing the formation of curly wing in D. melanogaster via changing the concentration of H2O2 and the temperature as well as the time of H2O2 treatment. We measured the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX), in order to examine the effects of H2O2 on antioxidative capacity of D. melanogaster. The results showed that the eclosion rate of D. melanogaster was inversely correlated with the concentration of H2O2. The change of temperature, H2O2 concentration and the period of H2O2 treatment affected the degree of the curl and the proportion of the curly wing. The white mutant flies responded most significantly to these three conditions, the mini-white (white gene reverse mutation) flies could rescue the curly phenotype, and responded similarly to the wild type OR. H2O2 had effects on the formation of the curly wing which contained the Cy mutation, leading to increased rate of the curly wing. D. melanogaster treated with H2O2 would reduce the antioxidative capacity. Results from real-time quantitative PCR showed that H2O2 treatment resulted in a change in gene expression. The formation of curly wing was a complicated process, and H2O2 might act as a signaling molecule or indirectly affect certain factors in the formation of curly wing in D. melano-gaster. This process might share the same signaling pathway with the Cy mutant, or might be regulated by different modulating patterns.

UV-visible spectroscopic study of the salicyladehyde benzoylhydrazone and its cobalt complexes.

Salicyladehyde benzoylhydrazone (SBH) has three groups suitable for forming coordination bond with transition metal. The UV-vis absorption spectra of SBH and its Co(II) complexes in various media were studied by using the deconvolution method. It is found that the structure of complex in solution is different from those in solid crystals. The nature of complexes in solution depends on acidity of the phenolic proton of SBH and on the medium. In neutral or slightly acid medium, the SBH is a non-charged bidentate ligand. And the "free" hydroxyl group on the SBH molecule makes it possible to form hydrogen bonds in solution. In basic medium, the SBH is a mono, negatively charged tridentates ligand.

[Study on the occurrence of ferrum in coal by ultrasound-assisted sequential chemical extraction].

To reveal the occurrence of Ferrum in coal, seven coal samples were selected according to the different contents of ferric oxide in the coal ash, and the content of Ferric element was determined by atomic absorption spectrophotometer (AAS) using nitric acid-perchloric acid-hydrofluoric acid wet digestion. Modes of occurrence of ferrium in the seven coal samples were studied by AAS using ultrasound-assisted sequential chemical extraction experiment (SCEE). Ultrasound promoted the contact of the extraction reagent with the coal particles and enhanced the dissolution process by producing characteristic acoustic cavitations, which greatly shortened the experimental time. The total amount of ferrium obtained by sequential extraction was approximate to the result of wet digestion, which indicated the procedure of SCEE was reasonable. The results showed that the ferric element mainly occurred in forms of carbonate state, Fe-Mn oxides state, sulfide state, sialic state and organic bound Fe in these coal samples. Among the various forms of these occurrences, the content of carbonate was the least, below 3.1%, while the content of sulfide was the dominant occurrence, ranging from 40% to 81.5%.