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1.
Opt Lett ; 49(15): 4266-4269, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-39090910

RESUMO

A stepped-frequency (SF) radar waveform generation method based on Fourier domain mode-locking (FDML) period-one laser dynamics is proposed and demonstrated. By fast controlling the optical injection strength of a semiconductor laser through electro-optical modulation, a broadband SF signal is generated. By further introducing an optoelectronic feedback loop with its round trip time delay matched with the temporal period of the SF signal, FDML is enabled through which the frequency stability, accuracy, and in-band signal-to-noise ratio are greatly improved. In the experiment, SF signals with a bandwidth of 6 GHz (12-18 GHz) and a frequency step of 150 MHz are generated. By comparing the qualities of signals generated with and without FDML, advantages of the proposed SF signal generation method are verified. Based on the proposed SF signal generation method, high-resolution inverse synthetic aperture radar (ISAR) imaging is also demonstrated, in which the 2D imaging resolution reaches 2.6 cm × 3.82 cm.

2.
Opt Lett ; 49(13): 3592-3595, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38950217

RESUMO

We propose and demonstrate a dual-band microwave photonic radar scheme based on a monolithic integrated mutual injection laser. Based on the photon-photon resonance (PPR) and the gain switching effect of the integrated laser, the C-/X-band triangular chirp signals with high-quality and comparable power at 4.75-5.25 GHz and 9.5-10.5 GHz are generated. In the current proof-of-concept experiment, the range resolution of the dual-band chirp signals can reach 16.9 cm, compared with the single-band chirp signal that cannot distinguish the targets. Through the application of a single integrated device and a transceiver module sharing a set of antennas, the dual-band microwave photonic radar system scheme improves the system integration.

3.
Circ Res ; 131(9): 768-787, 2022 10 14.
Artigo em Inglês | MEDLINE | ID: mdl-36134578

RESUMO

RATIONALE: Vascular smooth muscle cells (VSMCs) phenotype switch from contractile to proliferative phenotype is a pathological hallmark in various cardiovascular diseases. Recently, a subset of long noncoding RNAs was identified to produce functional polypeptides. However, the functional impact and regulatory mechanisms of long noncoding RNAs in VSMCs phenotype switching remain to be fully elucidated. OBJECTIVES: To illustrate the biological function and mechanism of a VSMC-enriched long noncoding RNA and its encoded peptide in VSMC phenotype switching and vascular remodeling. RESULTS: We identified a VSMC-enriched transcript encoded by a previously uncharacterized gene, which we called phenotype switching regulator (PSR), which was markedly upregulated during vascular remodeling. Although PSR was annotated as a long noncoding RNA, we demonstrated that the lncPSR (PSR transcript) also encoded a protein, which we named arteridin. In VSMCs, both arteridin and lncPSR were necessary and sufficient to induce phenotype switching. Mechanistically, arteridin and lncPSR regulate downstream genes by directly interacting with a transcription factor YBX1 (Y-box binding protein 1) and modulating its nuclear translocation and chromatin targeting. Intriguingly, the PSR transcription was also robustly induced by arteridin. More importantly, the loss of PSR gene or arteridin protein significantly attenuated the vascular remodeling induced by carotid arterial injury. In addition, VSMC-specific inhibition of lncPSR using adeno-associated virus attenuated Ang II (angiotensin II)-induced hypertensive vascular remodeling. CONCLUSIONS: PSR is a VSMC-enriched gene, and its transcript IncPSR and encoded protein (arteridin) coordinately regulate transcriptional reprogramming through a shared interacting partner, YBX1. This is a previously uncharacterized regulatory circuit in VSMC phenotype switching during vascular remodeling, with lncPSR/arteridin as potential therapeutic targets for the treatment of VSMC phenotype switching-related vascular remodeling.


Assuntos
RNA Longo não Codificante , Angiotensina II/metabolismo , Proliferação de Células/genética , Células Cultivadas , Cromatina/metabolismo , Humanos , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Fenótipo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Fatores de Transcrição/metabolismo , Remodelação Vascular
4.
Circulation ; 146(14): 1082-1095, 2022 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-36004643

RESUMO

BACKGROUND: Adverse environmental exposure during the prenatal period can lead to diseases in the offspring, including hypertension. Whether or not the hypertensive phenotype can be transgenerationally transmitted is not known. METHODS: Pregnant Sprague Dawley rats were intraperitoneally injected with lipopolysaccharide (LPS) on gestation days 6, 8, 10, and 12 to generate the prenatal LPS exposure model. Blood pressure was monitored by both telemetry and tail-cuff method. RNA sequencing was performed to analyze transcriptome alteration in the kidney of the third generation. Tempol and spironolactone were used to test the potential preventative and therapeutic effect of targeting reactive oxygen species and mineralocorticoid receptor signaling, respectively. Molecular biological experiments were performed to illustrate the mechanism of epigenetic and transcription regulation. RESULTS: Prenatal LPS exposure can impair the ability to excrete a salt load and induce hypertension from the first to the third generations, with the fourth and fifth generations, inducing salt-sensitive hypertension. Compared with control pups, the transcriptome in the kidney of the hypertensive third-generation prenatal LPS-exposed offspring have upregulation of the Ras-related C3 botulinum toxin substrate 1 (Rac1) gene and activation of mineralocorticoid receptor signaling. Furthermore, we found that LPS exposure during pregnancy triggered oxidative stress that upregulated KDM3B (histone lysine demethylase 3B) in the oocytes of first-generation female rats, leading to an inheritable low level of H3K9me2 (histone H3 lysine 9 dimethylation), resulting in the transgenerational upregulation of Rac1. Based on these findings, we treated the LPS-exposed pregnant rats with the reactive oxygen species scavenger, tempol, which successfully prevented hypertension in the first-generation offspring and the transgenerational inheritance of hypertension. CONCLUSIONS: These findings show that adverse prenatal exposure induces transgenerational hypertension through an epigenetic-regulated mechanism and identify potentially preventive and therapeutic strategies for hypertension.


Assuntos
Hipertensão , Efeitos Tardios da Exposição Pré-Natal , Animais , Óxidos N-Cíclicos , Feminino , Histona Desmetilases , Histonas , Hipertensão/induzido quimicamente , Hipertensão/genética , Histona Desmetilases com o Domínio Jumonji , Lipopolissacarídeos/toxicidade , Lisina , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/etiologia , Ratos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio , Receptores de Mineralocorticoides/genética , Marcadores de Spin , Espironolactona , Proteínas rac1 de Ligação ao GTP/genética
5.
Opt Express ; 31(26): 42744-42753, 2023 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-38178386

RESUMO

A novel photonic method of linearly frequency-modulated (LFM) signal generation with high purity based on the monolithically integrated semiconductor laser (MISL) subject to the dynamical optoelectrical feedback is proposed and demonstrated in this paper. In this approach, the MISL is firstly operated in period-one state. By introducing the dynamical optoelectrical feedback to modulate the MISL, the generated LFM signals would be constantly optimized as long as the delay of the feedback loop is matched with the repetition period of the LFM signal. In this system, no additional high-speed external modulator, high-frequency electrical LFM oscillator are required, highly simplifying the framework and reducing the power consumption. In the current proof-of-concept experiment, one LFM signal with the bandwidth as large as 5.6 GHz is generated and the corresponding frequency comb contrast can be drastically improved by 51 dB. Furthermore, the effect of the delay mismatch is also discussed in this paper.

6.
Appl Opt ; 62(21): 5613-5618, 2023 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-37707176

RESUMO

We propose a new, to the best of our knowledge, broadband signal downconversion scheme implemented by a monolithic integrated mutual injection laser. A mathematical derivation, simulation, and experimental verification are carried out. Because the period-one oscillation frequency can be selectively operated on a large scale by controlling the current on the integrated laser, the tuning downconversion range is realized without changing the experimental equipment. The experiment verifies that the downconversion of the linear frequency modulation signal with a bandwidth of 0.5 GHz from the center frequency of 18.75 to 0.85 GHz, and the spurious-free dynamic range (SFDR) has reached 71.7d B/H z 2/3. Compared with the scheme based on discrete components, the system has no electric local oscillator or external modulator, which provides a method for radar signal downconversion.

7.
Appl Opt ; 62(28): 7463-7470, 2023 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-37855515

RESUMO

To break the dependence on a high-speed and high-resolution digital-to-analog converter (DAC) in the traditional quantum noise randomized cipher (QNRC), a practical DAC-free modulation scheme based on cascaded phase-shift keying (PSK) is proposed and demonstrated by a proof-of-concept experiment. By employing seven cascaded phase modulators (PMs) driven by designed electrical voltage signals, a 128 PSK-QNRC system is achieved with a transmission rate of 10 Gbaud/s and a transmission distance more than 50 km, which eliminates the need for a DAC on the transmitter side. The bit error rate (BER) performance of the proposed scheme is compared to that of a traditional scheme based on an arbitrary waveform generator (AWG) with a sampling rate of 25 GSa/s. The results show that compared to a traditional scheme, the power penalties of the proposed scheme are -1.8d B, 0.9 dB, and 1 dB, respectively, at the rates of 10, 5, and 2.5 Gbps. In other words, the BER performance of the proposed scheme is close to the traditional scheme at a low transmission rate, but better than that of the traditional scheme at a high transmission rate, where the sampling rate of the DAC is not high enough to generate a complete waveform. This work greatly enhances the security of a QNRC system.

8.
Appl Opt ; 62(7): 1689-1694, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-37132915

RESUMO

A simple and highly efficient four-channel all-optical wavelength conversion based on four-wave mixing effect of the directly modulated three-section monolithically integrated semiconductor laser is proposed and experimentally investigated. For this wavelength conversion unit, the spacing of the wavelength can be adjusted by tuning the bias current of the lasers and setting it to be 0.4 nm (50 GHz) as a demonstration is this work. A 50 Mbps 16-QAM signal centers at 4-8 GHz is experimentally switched to a targeted path. Up- or downconversion depends on a wavelength-selective switch, and the conversion efficiency can reach up to -2 to 0 dB. This work provides a new technology for photonic radio-frequency switching matrix and contributes to the integrated implementation of satellite transponders.

9.
Appl Opt ; 62(7): 1822-1828, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-37132931

RESUMO

Multi-band linearly frequency-modulated (LFM) signal generation with a multiplying bandwidth is proposed and experimentally demonstrated. It is a simple photonics method based on the gain-switching state in a distributed feedback semiconductor laser without a complex external modulator and high-speed electrical amplifiers. With N comb lines, the carrier frequency and bandwidth of generated LFM signals are N times those of the reference signal. (N is the number of comb lines.) The number of bands and time-bandwidth products (TBWPs) of the generated signals could be easily adjusted by tuning the reference signal from an arbitrary waveform generator. Three-band LFM signals with carrier frequencies ranging from the X-band to K-band are given as an example, and the TBWP up to 20000. The results of auto-correlations of the generated waveforms are also given.

10.
Circulation ; 143(20): 2007-2022, 2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-33663221

RESUMO

BACKGROUND: Heart failure (HF) is among the leading causes of morbidity and mortality, and its prevalence continues to rise. LARP7 (La ribonucleoprotein domain family member 7) is a master regulator that governs the DNA damage response and RNAPII (RNA polymerase II) pausing pathway, but its role in HF pathogenesis is incompletely understood. METHODS: We assessed LARP7 expression in human HF and in nonhuman primate and mouse HF models. To study the function of LARP7 in heart, we generated global and cardiac-specific LARP7 knockout mice. We acutely abolished LARP7 in mature cardiomyocytes by Cas9-mediated LARP7 somatic knockout. We overexpressed LARP7 in cardiomyocytes using adeno-associated virus serotype 9 and ATM (ataxia telangiectasia mutated protein) inhibitor. The therapeutic potential of LARP7-regulated pathways in HF was tested in a mouse myocardial infarction model. RESULTS: LARP7 was profoundly downregulated in failing human hearts and in nonhuman primate and murine hearts after myocardial infarction. Low LARP7 levels in failing hearts were linked to elevated reactive oxygen species, which activated the ATM-mediated DNA damage response pathway and promoted LARP7 ubiquitination and degradation. Constitutive LARP7 knockout in mouse resulted in impaired mitochondrial biogenesis, myocardial hypoplasia, and midgestational lethality. Cardiac-specific inactivation resulted in defective mitochondrial biogenesis, impaired oxidative phosphorylation, elevated oxidative stress, and HF by 4 months of age. These abnormalities were accompanied by reduced SIRT1 (silent mating type information regulation 2 homolog 1) stability and deacetylase activity that impaired SIRT1-mediated transcription of genes for oxidative phosphorylation and energy metabolism and dampened cardiac function. Restoring LARP7 expression after myocardial infarction by either adeno-associated virus-mediated LARP7 expression or small molecule ATM inhibitor substantially improved the function of injured heart. CONCLUSIONS: LARP7 is essential for mitochondrial biogenesis, energy production, and cardiac function by modulating SIRT1 homeostasis and activity. Reduction of LARP7 in diseased hearts owing to activation of the ATM pathway contributes to HF pathogenesis and restoring LARP7 in the injured heart confers myocardial protection. These results identify the ATM-LARP7-SIRT1 pathway as a target for therapeutic intervention in HF.


Assuntos
Insuficiência Cardíaca/genética , Mitocôndrias/metabolismo , Ribonucleoproteínas/metabolismo , Animais , Modelos Animais de Doenças , Humanos , Camundongos , Biogênese de Organelas
11.
Microvasc Res ; 133: 104076, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32956647

RESUMO

The adverse environment in early life can modulate adult phenotype, including blood pressure. Our previous study shows, in a rat streptozotocin (STZ)-induced maternal diabetes model, fetal exposure to maternal diabetes is characterized by established hypertension in the offspring. However, the exact mechanisms are not known. Our present study found, as compared with male control mother offspring (CMO), male diabetic mother offspring (DMO) had higher blood pressure with arterial dysfunction, i.e., decreased acetylcholine (Ach)-induced vasodilation. But there is no difference in blood pressure between female CMO and DMO. The decreased Ach-induced vasodilation was related to decreased nitric oxide (NO) production in the endothelium, not NO sensitivity in vascular smooth muscle because sodium nitroprusside (SNP)-mediated vasodilation was preserved; there was decreased NO production and lower eNOS phosphorylation in male DMO. The reactive oxygen species (ROS) level was increased in male DMO than CMO; normalized ROS levels with tempol increased NO production, normalized Ach-mediated vasodilation, and lowered blood pressure in male DMO rats. It indicates that diabetic programming hypertension is related to arterial dysfunction; normalizing ROS might be a potential strategy for the prevention of hypertension in the offspring.


Assuntos
Diabetes Mellitus Experimental/complicações , Diabetes Gestacional , Endotélio Vascular/fisiopatologia , Hipertensão/etiologia , Artéria Mesentérica Superior/fisiopatologia , Efeitos Tardios da Exposição Pré-Natal , Fatores Etários , Animais , Pressão Arterial , Glicemia/metabolismo , GMP Cíclico/metabolismo , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/fisiopatologia , Diabetes Gestacional/sangue , Diabetes Gestacional/fisiopatologia , Endotélio Vascular/metabolismo , Feminino , Hipertensão/metabolismo , Hipertensão/fisiopatologia , Masculino , Artéria Mesentérica Superior/metabolismo , Óxido Nítrico/metabolismo , Estresse Oxidativo , Gravidez , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Fatores Sexuais , Vasodilatação
12.
Clin Sci (Lond) ; 135(2): 409-427, 2021 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-33458737

RESUMO

Hypertensive nephropathy (HN) is a common cause of end-stage renal disease with renal fibrosis; chronic kidney disease is associated with elevated serum gastrin. However, the relationship between gastrin and renal fibrosis in HN is still unknown. We, now, report that mice with angiotensin II (Ang II)-induced HN had increased renal cholecystokinin receptor B (CCKBR) expression. Knockout of CCKBR in mice aggravated, while long-term subcutaneous infusion of gastrin ameliorated the renal injury and interstitial fibrosis in HN and unilateral ureteral obstruction (UUO). The protective effects of gastrin on renal fibrosis can be independent of its regulation of blood pressure, because in UUO, gastrin decreased renal fibrosis without affecting blood pressure. Gastrin treatment decreased Ang II-induced renal tubule cell apoptosis, reversed Ang II-mediated inhibition of macrophage efferocytosis, and reduced renal inflammation. A screening of the regulatory factors of efferocytosis showed involvement of peroxisome proliferator-activated receptor α (PPAR-α). Knockdown of PPAR-α by shRNA blocked the anti-fibrotic effect of gastrin in vitro in mouse renal proximal tubule cells and macrophages. Immunofluorescence microscopy, Western blotting, luciferase reporter, and Cut&tag-qPCR analyses showed that CCKBR may be a transcription factor of PPAR-α, because gastrin treatment induced CCKBR translocation from cytosol to nucleus, binding to the PPAR-α promoter region, and increasing PPAR-α gene transcription. In conclusion, gastrin protects against HN by normalizing blood pressure, decreasing renal tubule cell apoptosis, and increasing macrophage efferocytosis. Gastrin-mediated CCKBR nuclear translocation may make it act as a transcription factor of PPAR-α, which is a novel signaling pathway. Gastrin may be a new potential drug for HN therapy.


Assuntos
Gastrinas/farmacologia , Hipertensão Renal/fisiopatologia , Nefrite/fisiopatologia , PPAR alfa/metabolismo , Receptores da Colecistocinina/metabolismo , Angiotensina II/administração & dosagem , Animais , Apoptose , Fibrose , Humanos , Hipertensão/complicações , Células Jurkat , Túbulos Renais Proximais/patologia , Camundongos , Camundongos Knockout , PPAR alfa/genética , Fagocitose , RNA Interferente Pequeno , Receptores da Colecistocinina/genética , Transdução de Sinais/efeitos dos fármacos , Obstrução Ureteral/fisiopatologia
13.
Exp Cell Res ; 349(1): 179-183, 2016 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-27751837

RESUMO

Extracellular vesicles (EVs) are small membrane vesicles including exosomes and shedding vesicles that mediated a cell-to-cell communication. EVs are released from almost all cell types under both physiological and pathological conditions and incorporate nuclear and cytoplasmic molecules for intercellular delivery. Besides protein, mRNA, and microRNA of these molecules, as recent studies show, specific DNA are prominently packaged into EVs. It appears likely that some of exosomes or shedding vesicles, bearing nuclear molecules are released upon bubble-like blebs. Specific interaction of EVs with susceptible recipients performs the uptake of EVs into the target cells, discharging their cargo including nuclear and cytoplasmic macromolecules into the cytosol. These findings expand the nucleic acid content of EVs to include increased levels of specific DNA. Thus, EVs contain a repertoire of genetic information available for horizontal gene transfer and potential use as blood biomarkers for cancer and atherosclerosis. In this review, the focus is on the characteristics, biological functions, and roles in diseases of DNA within EVs.


Assuntos
DNA/metabolismo , Vesículas Extracelulares/metabolismo , Animais , Doença , Técnicas de Transferência de Genes , Humanos , Modelos Biológicos
14.
Clin Sci (Lond) ; 130(24): 2279-2293, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27682003

RESUMO

Cardiac troponin I (cTnI), a biomarker for myocardial damage and risk stratification, may be involved in the pathogenesis of cardiovascular diseases, which was ascribed to the effect of cTnI auto-antibodies. Whether or not cTnI itself has a direct impact on acute myocardial injury is unknown. To exclude the influence of cTnI antibody on the cardiac infarct size, we studied the effect of cTnI shortly after myocardial ischaemia-reperfusion (I/R) injury when cTnI antibodies were not elevated. Pretreatment with cTnI augmented the myocardial infarct size caused by I/R, accompanied by an increase in inflammatory markers in the blood and myocardium. Additional experiments using human umbilical vein endothelial cells (HUVECs) showed that the detrimental effect of cTnI was related to cTnI-induced increase in vascular cell adhesion molecule-1 (VCAM-1) expression and VCAM-1 mediated adhesion of human monocytes (THP-1) to HUVECs, which could be neutralized by VCAM-1 antibody. Both toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) were involved in the signalling pathway, because blockade of either TLR4 or NF-κB inhibited the cTnI's effect on VCAM-1 expression and adhesion of monocytes to endothelial cells. Moreover, TLR4 inhibition reduced cTnI-augmented cardiac injury in rats with I/R injury. We conclude that cTnI exacerbates myocardial I/R injury by inducing the adhesion of monocytes to vascular endothelial cells via activation of the TLR4/NF-κB pathway. Inhibition of TLR4 may be an alternative strategy to reduce cTnI-induced myocardial I/R injury.

15.
Biochim Biophys Acta ; 1839(2): 88-96, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24412852

RESUMO

The aberrant activation of telomerase is critical for the initiation and development of human cervical cancer, which is dependent on the activation of human telomerase reverse transcriptase (hTERT). Recently, Pin2/TRF1-interacting protein X1 (PinX1) has been identified as a suppressor of hTERT. It has been found that the telomerase is activated while the level of PinX1 is decreased in cervical cancer. However, the regulatory mechanism of PinX1 in cervical cancer cells remains unclear. In the present study, we demonstrated that the level of PinX1 is regulated by p53, and p53 functions as a transcriptional factor to directly activate the expression of PinX1 in cervical cancer cells. Moreover, we found that HPV16 E6 suppresses the expression of PinX1 via inhibiting p53 transcriptional activity, resulting in the enhancement of telomerase activity. This study not only for the first time shows that PinX1 is a novel target gene of p53 but also suggests that suppression of p53/PinX1 pathway may be a novel mechanism by which HPV16 E6 enhances the telomerase activity in cervical cancer cells.


Assuntos
Proteínas Oncogênicas Virais/genética , Proteínas Repressoras/genética , Proteína Supressora de Tumor p53/genética , Proteínas Supressoras de Tumor/genética , Western Blotting , Proteínas de Ciclo Celular , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Mutação , Proteínas Oncogênicas Virais/metabolismo , Regiões Promotoras Genéticas/genética , Ligação Proteica , Interferência de RNA , Proteínas Repressoras/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Telomerase/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/patologia
16.
Circulation ; 130(17): 1452-1465, 2014 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-25156994

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. METHODS AND RESULTS: We identified lincRNA-p21 as a key regulator of cell proliferation and apoptosis during atherosclerosis. The expression of lincRNA-p21 was dramatically downregulated in atherosclerotic plaques of ApoE(-/-) mice, an animal model for atherosclerosis. Through loss- and gain-of-function approaches, we showed that lincRNA-p21 represses cell proliferation and induces apoptosis in vascular smooth muscle cells and mouse mononuclear macrophage cells in vitro. Moreover, we found that inhibition of lincRNA-p21 results in neointimal hyperplasia in vivo in a carotid artery injury model. Genome-wide analysis revealed that lincRNA-p21 inhibition dysregulated many p53 targets. Furthermore, lincRNA-p21, a transcriptional target of p53, feeds back to enhance p53 transcriptional activity, at least in part, via binding to mouse double minute 2 (MDM2), an E3 ubiquitin-protein ligase. The association of lincRNA-p21 and MDM2 releases MDM2 repression of p53, enabling p53 to interact with p300 and to bind to the promoters/enhancers of its target genes. Finally, we show that lincRNA-p21 expression is decreased in patients with coronary artery disease. CONCLUSIONS: Our studies identify lincRNA-p21 as a novel regulator of cell proliferation and apoptosis and suggest that this lncRNA could serve as a therapeutic target to treat atherosclerosis and related cardiovascular disorders.


Assuntos
Apoptose/genética , Macrófagos/citologia , Músculo Liso Vascular/citologia , Neointima/genética , RNA Longo não Codificante/genética , Proteína Supressora de Tumor p53/metabolismo , Animais , Aterosclerose/genética , Aterosclerose/metabolismo , Aterosclerose/patologia , Doenças das Artérias Carótidas/genética , Doenças das Artérias Carótidas/metabolismo , Doenças das Artérias Carótidas/patologia , Linhagem Celular , Proliferação de Células , Modelos Animais de Doenças , Humanos , Macrófagos/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Músculo Liso Vascular/fisiologia , Neointima/patologia , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteína Supressora de Tumor p53/genética
17.
Clin Sci (Lond) ; 129(8): 675-85, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26201019

RESUMO

Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery disease (CAD), biomarker is not known. The present study screened lncRNAs by microarray analysis in the plasma from CAD patients and control individuals and found that 265 lncRNAs were differentially expressed. To find specific lncRNAs as possible CAD biomarker candidates, we used the following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5 and P<0.005. According to these criteria, five intergenic lncRNAs were identified. After validation by quantitative PCR (qPCR), one lncRNA was excluded from the candidate list. The remaining four lncRNAs were independently validated in another population of 20 CAD patients and 20 control individuals. Receiver operating characteristic (ROC) curve analysis showed that lncRNA AC100865.1 (referred to as CoroMarker) was the best of these lncRNAs. CoroMarker levels were also stable in plasma. The predictive value of CoroMarker was further assessed in a larger cohort with 221 CAD patients and 187 control individuals. Using a diagnostic model with Fisher's criteria, taking the risk factors into account, the optimal sensitivity of CoroMarker for CAD increased from 68.29% to 78.05%, whereas the specificity decreased slightly from 91.89% to 86.49%. CoroMarker was stable in plasma because it was mainly in the extracellular vesicles (EVs), probably from monocytes. We conclude that CoroMarker is a stable, sensitive and specific biomarker for CAD.


Assuntos
Doença da Artéria Coronariana/sangue , RNA não Traduzido/sangue , Idoso , Biomarcadores/sangue , Estudos de Casos e Controles , Doença da Artéria Coronariana/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Fatores de Risco
18.
Interdiscip Sci ; 16(1): 104-122, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-37976024

RESUMO

Vascular disease is one of the major causes of death worldwide. Endothelial cells are important components of the vascular structure. A better understanding of the endothelial cell changes in the development of vascular disease may provide new targets for clinical treatment strategies. Single-cell RNA sequencing can serve as a powerful tool to explore transcription patterns, as well as cell type identity. Our current study is based on comprehensive scRNA-seq data of several types of human vascular disease datasets with deep-learning-based algorithm. A gene set scoring system, created based on cell clustering, may help to identify the relative stage of the development of vascular disease. Metabolic preference patterns were estimated using a graphic neural network model. Overall, our study may provide potential treatment targets for retaining normal endothelial function under pathological situations.


Assuntos
Perfilação da Expressão Gênica , Doenças Vasculares , Humanos , Células Endoteliais , Análise de Sequência de RNA , Análise da Expressão Gênica de Célula Única , Algoritmos , Artérias , Análise por Conglomerados
19.
Theranostics ; 14(4): 1450-1463, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38389849

RESUMO

Aims: Smooth muscle cell (SMC) remodeling poses a critical feature in the development and progression of atherosclerosis. Although fate mapping and in silicon approaches have expanded SMC phenotypes in atherosclerosis, it still remains elusive about the contributions of individual SMC phenotypes and molecular dynamics to advanced atherosclerotic plaque. Methods: Using single-cell transcriptome, we investigated cellular compositions of human carotid plaque laden with atherosclerotic core, followed by in vivo experiments utilizing SMC-lineage tracing technology, bulk RNA sequencing (RNA-seq) and both in vivo and in vitro validation of the underlying molecular mechanism. Results: 5 functionally distinct SMC subtypes were uncovered based on transcriptional features (described as contractile, fibroblast-like, osteogenic, synthetic and macrophage-like) within the niche. A proinflammatory, macrophage-like SMC subtype displaying an intermediary phenotype between SMC and macrophage, exhibits prominent potential in destabilizing plaque. At the molecular level, we explored cluster-specific master regulons by algorithm, and identified interferon regulatory factor-8 (IRF8) as a potential stimulator of SMC-to-macrophage transdifferentiation via activating nuclear factor-κB (NF-κB) signaling. Conclusions: Our study illustrates a comprehensive cell atlas and molecular landscape of advanced atherosclerotic lesion, which might renovate current understanding of SMC biology in atherosclerosis.


Assuntos
Aterosclerose , Placa Aterosclerótica , Humanos , Placa Aterosclerótica/genética , Placa Aterosclerótica/patologia , Aterosclerose/genética , Aterosclerose/patologia , Perfilação da Expressão Gênica , Miócitos de Músculo Liso/patologia , Macrófagos/patologia
20.
Sci Rep ; 14(1): 19040, 2024 08 16.
Artigo em Inglês | MEDLINE | ID: mdl-39152148

RESUMO

Protein-encoding circular RNAs (circRNAs) are newly identified RNA molecules characterized by intense interaction with translating ribosome. Emerging evidence has implicated physiological and pathological significance of these non-canonical RNAs, yet a large body of them remains unidentified. Due to limited tools at hand, we developed CircProPlus, an automated computational pipeline for de novo detection of translated circRNAs. In comparison to previously established CircPro, CircProPlus adjusts the overall workflow and integrates more robust implements for achieving easier accessibility, higher flexibility and productivity. In present study, we tested the performance of CircProPlus when using different circRNA-detecting implements (i.e., CIRI2, CirComPara2) in the evaluation of coding ability of circRNAs. Results showed that CirComPara2, a state-of-the-art algorithm, consistently outperformed CIRI2 when coupled with CircProPlus in testing real data collected from different RNA libraries and species, which highlighted its potency in data mining of circRNAs with protein-coding potential.


Assuntos
Algoritmos , RNA Circular , RNA Circular/genética , Humanos , Biologia Computacional/métodos , Software
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