RESUMO
Modern imaging methods are applied extensively in translational animal research, and combined analysis of anatomical and functional imaging results is of increasing importance. Many imaging centers handle multiple independent animal colonies and use several imaging modalities, often in combination. The authors have developed and successfully tested a two-piece acrylic Multimodality Chamber that enables investigators to coregister images from two or more modalities, including microMR, microCT, microPET and optical imaging.
Assuntos
Interpretação de Imagem Assistida por Computador , Tomografia por Emissão de Pósitrons/instrumentação , Tomografia Computadorizada por Raios X/instrumentação , Imagem Corporal Total/instrumentação , Animais , Modelos Animais de Doenças , Feminino , Camundongos , Tomografia por Emissão de Pósitrons/métodos , Software , Tomografia Computadorizada por Raios X/métodos , Imagem Corporal Total/métodosRESUMO
The unprecedented increase in preclinical studies necessitates high-throughput, inexpensive, and straightforward methods for evaluating diseased tissues. Near-infrared imaging of live subjects is a versatile, cost-effective technology that can be effectively used in a variety of pathologic conditions. We have characterized an inexpensive optoelectronic chemical, IR-820, as an infrared blood pool contrast agent to detect and quantify diseased tissue in live animals. IR-820 has maximal excitation and emission wavelengths of 710 and 820 nm, respectively. IR-820 emission is significantly improved in vivo on serum binding to albumin, and elimination occurs predominantly via the gastrointestinal tract. We demonstrate the utility of this contrast agent for serially imaging of traumatized tissue (muscle), tissue following reperfusion (eg, stroke), and tumors. IR-820 can also be employed to map regional lymph nodes. This novel contrast agent is anticipated to be a useful and an inexpensive tool for screening a wide variety of preclinical models of human diseases.
Assuntos
Meios de Contraste/farmacocinética , Verde de Indocianina/análogos & derivados , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Ferimentos e Lesões/diagnóstico , Animais , Permeabilidade Capilar/fisiologia , Verde de Indocianina/farmacocinética , Taxa de Depuração Metabólica/fisiologia , Camundongos , Camundongos Pelados , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Modelos Biológicos , Recuperação de Função Fisiológica/fisiologia , Fatores de Tempo , Ferimentos e Lesões/metabolismo , Ferimentos e Lesões/reabilitaçãoRESUMO
Forward and reverse genetics now allow researchers to understand embryonic and postnatal gene function in a broad range of species. Although some genetic mutations cause obvious morphological change, other mutations can be more subtle and, without adequate observation and quantification, might be overlooked. For the increasing number of genetic model organisms examined by the growing field of phenomics, standardized but sensitive methods for quantitative analysis need to be incorporated into routine practice to effectively acquire and analyze ever-increasing quantities of phenotypic data. In this study, we present platform-independent parameters for the use of microscopic x-ray computed tomography (microCT) for phenotyping species-specific skeletal morphology of a variety of different genetic model organisms. We show that microCT is suitable for phenotypic characterization for prenatal and postnatal specimens across multiple species.