Quantitative Proteomics Reveal the Inherent Antibiotic Resistance Mechanism against Norfloxacin Resistance in Aeromonas hydrophila.

Recently, the prevalence of Aeromonas hydrophila antibiotic-resistant strains has been reported in aquaculture, but its intrinsic antibiotic resistance mechanisms are largely unknown. In the present study, a label-free proteomics technology was used to compare the differential protein abundances in response to norfloxacin (NOR) stress in A. hydrophila. The results showed that there were 186 proteins decreasing and 220 proteins increasing abundances in response to NOR stress. Bioinformatics analysis showed that the differentially expressed proteins were enriched in several biological processes, such as sulfur metabolism and homologous recombination. Furthermore, the antibiotic sensitivity assays showed that the deletion of AHA_0904, cirA, and cysI significantly decreased the resistance against NOR, whereas ΔAHA_1239, ΔcysA, ΔcysD, and ΔcysN significantly increased the resistance against NOR. Our results provide insights into NOR resistance mechanisms and indicate that AHA_0904, cirA, AHA_1239, and sulfur metabolism may play important roles in NOR resistance in A. hydrophila.

Pretreatment of Ascorbic Acid Inhibits MPTP-Induced Astrocytic Oxidative Stress through Suppressing NF-κB Signaling.

Astrocytes are a major constituent of the central nervous system (CNS). Astrocytic oxidative stress contributes to the development of Parkinson's disease (PD). Maintaining production of antioxidant and detoxification of reactive oxygen and nitrogen species (ROS/RNS) in astrocytes is critical to prevent PD. Study has illuminated that ascorbic acid (AA) stimulates dopamine synthesis and expression of tyrosine hydroxylase in human neuroblastoma cells. However, the role and regulatory mechanisms of AA on detoxification of astrocytes are still unclear. The purpose of our study is in-depth study of the regulatory mechanism of AA on detoxification of astrocytes. We found that AA pretreatment decreased the expression of ROS and inducible nitric oxide synthase (iNOS) in MPP+-treated astrocytes. In contrast, the expression levels of antioxidative substances-including superoxide dismutase (SOD), glutathione (GSH), and glutamate-cysteine ligase modifier (GCLM) subunit-were upregulated after AA pretreatment in MPP+-treated astrocytes. However, inhibition of NF-κB prevented such AA induced increases in antioxidative substances following MPP+ treatment in astrocytes, suggesting that AA improved antioxidative function of astrocytes through inhibiting NF-κB-mediated oxidative stress. Furthermore, in vivo studies revealed that AA preadministration also suppressed NF-κB and upregulated the expression levels of antioxidative substances in the midbrain of MPTP-treated mice. Additionally, pretreatment of AA alleviated MPTP-induced PD-like pathology in mice. Taken together, our results demonstrate that preadministration of AA improves the antioxidative function of astrocytes through suppressing NF-κB signaling, following alleviated the pathogenesis of PD which induced by MPTP. Hence, our findings elucidate a novel protective mechanism of AA in astrocytes.

Bioinspired Design Provides High-Strength Benzoxazine Structural Adhesives.

A synthetic strategy to incorporate catechol functional groups into benzoxazine thermoset monomers was developed, leading to a family of bioinspired small-molecule resins and main-chain polybenzoxazines derived from biologically available phenols. Lap-shear adhesive testing revealed a polybenzoxazine derivative with greater than 5 times improved shear strength on aluminum substrates compared to a widely studied commercial benzoxazine resin. Derivative synthesis identified the catechol moiety as an important design feature in the adhesive performance and curing behavior of this bioinspired thermoset. Favorable mechanical properties comparable to commercial resin were maintained, and glass transition temperature and char yield under nitrogen were improved. Blending of monomers with bioinspired main-chain polybenzoxazine derivatives provided formulations with enhanced shear adhesive strengths up to 16 MPa, while alloying with commercial core-shell particle-toughened epoxy resins led to shear strengths exceeding 20 MPa. These results highlight the utility of bioinspired design and the use of biomolecules in the preparation of high-performance thermoset resins and adhesives with potential utility in transportation and aerospace industries and applications in advanced composites synthesis.

Alterations of NMDA receptor binding in various brain regions among 6-hydroxydopamine-induced Parkinsonian rats.

The N-methyl-d-aspartate (NMDA) system closely interacts with the dopaminergic system and is strongly implicated in the pathophysiological mechanisms and therapeutic paradigms of Parkinson's disease. This study aims to systematically investigate the changes of NMDA receptors in a wide range of brain structures 3 weeks after unilateral medial forebrain bundle lesion by 6-hydroxydopamine (6-OHDA). NMDA receptor distributions and alterations in the post-mortem rat brain were detected by [(3)H] MK-801 binding autoradiography. In the 6-OHDA-induced Parkinsonian rat model, nigrostriatal dopaminergic neuron loss significantly mediated the decreased [(3)H] MK-801 binding, predominantly in the hippocampus (-22.4%, p < 0.001), caudate putamen (-14.1%, p < 0.01), accumbens nucleus (-13.8%, p < 0.05), cingulate cortex (-13.4%, p < 0.001), posteromedial cortical amygdala (-14.5%, p < 0.01) and piriform cortex (-9%, p < 0.05) compared to the controls, while there was a profound reduction of tyrosine hydroxylase (TH) immunohistochemistry in the substantia nigra pars compacta. Alterations in [(3)H] MK-801 in the specific brain regions related to cognitive functions may indicate that cognitive dysfunctions caused by 6-OHDA lesion were via the NMDA system. The downregulation of NMDA receptor binding in the present study provides indirect evidence for plasticity in the NMDA system in the rat brain. The present study improves our understanding of the critical roles of the NMDA receptors in treating neurodegenerative disorders, and implicates NMDA receptors as a novel therapeutic target in the treatment of Parkinson's disease.

SiO2@AuAg/PDA hybrid nanospheres with photo-thermally enhanced synergistic antibacterial and catalytic activity.

Wastewater discharged from industrial, agricultural and livestock production contains a large number of harmful bacteria and organic pollutants, which usually cause serious harm to human health. Therefore, it is urgent to find a "one-stone-two-birds" strategy with good antimicrobial and pollutant degradation activity for treating waste water. In this paper, SiO2@AuAg/Polydopamine (SiO2@AuAg/PDA) core/shell nanospheres, which possessed synergistic "Ag+-release-photothermal" antibacterial and catalytic behaviors, have been successfully prepared via a simple in situ redox polymerization method. The SiO2@AuAg/PDA nanospheres showed good catalytic activity in reducing 4-nitrophenol to 4-aminophenol (0.576 min-1 mg-1). Since the AuAg nanoclusters contain both gold and silver elements, they provided a high photothermal conversion efficiency (48.1%). Under NIR irradiation (808 nm, 2.5 W-2), the catalytic kinetics were improved by 2.2 times. Besides the intrinsic Ag+-release, the photothermal behavior originating from the AuAg bimetallic nanoclusters and the PDA component of SiO2@AuAg/PDA also critically improved the antibacterial performance. Both E. coli and S. aureus could be basically killed by SiO2@AuAg/PDA nanospheres at a concentration of 90 µg mL-1 under NIR irradiation. This "Ag+-release-photothermal" coupled sterilization offers a straightforward and effective approach to antimicrobial therapy, and further exhibits high potential in nanomedicine for combating bacterial contamination in environmental treatment and biological fields.

Gate-Tunable Positive and Negative Photoconductance in Near-Infrared Organic Heterostructures for In-Sensor Computing.

The rapid growth of sensor data in the artificial intelligence often causes significant reductions in processing speed and power efficiency. Addressing this challenge, in-sensor computing is introduced as an advanced sensor architecture that simultaneously senses, memorizes, and processes images at the sensor level. However, this is rarely reported for organic semiconductors that possess inherent flexibility and tunable bandgap. Herein, an organic heterostructure that exhibits a robust photoresponse to near-infrared (NIR) light is introduced, making it ideal for in-sensor computing applications. This heterostructure, consisting of partially overlapping p-type and n-type organic thin films, is compatible with conventional photolithography techniques, allowing for high integration density of up to 520 devices cm-2 with a 5 µm channel length. Importantly, by modulating gate voltage, both positive and negative photoresponses to NIR light (1050 nm) are attained, which establishes a linear correlation between responsivity and gate voltage and consequently enables real-time matrix multiplication within the sensor. As a result, this organic heterostructure facilitates efficient and precise NIR in-sensor computing, including image processing and nondestructive reading and classification, achieving a recognition accuracy of 97.06%. This work serves as a foundation for the development of reconfigurable and multifunctional NIR neuromorphic vision systems.

Serum uric acid in patients with Parkinson's disease and vascular parkinsonism: a cross-sectional study.

BACKGROUND: Elevation of serum uric acid (UA) is correlated with a decreased risk of Parkinson's disease (PD); however, the association and clinical relevance of serum UA levels in patients with PD and vascular parkinsonism (VP) are unknown. OBJECTIVE: We performed a cross-sectional study of 160 Chinese patients with PD and VP to determine whether UA levels in patients could predict the outcomes. METHODS: Serum UA levels were divided into quartiles and the association between UA and the severity of PD or VP was investigated in each quartile. RESULTS: The serum levels of UA in PD were significantly lower than those in normal subjects and VP. The serum UA levels in PD patients were significantly correlated with some clinical parameters. Strong correlations were observed in male PD patients, but significant correlations were observed only between UA and the non-motor symptoms (NMS) of burden of sleep/fatigue and mood in female PD patients. PD patients in the lowest quartile of serum UA levels had significant correlations between UA and the unified Parkinson's disease rating scale, the modified Hoehn and Yahr staging scale and NMS burden for attention/memory. CONCLUSION: Our findings support the hypothesis that subjects with low serum UA levels may be more prone to developing PD and indicate that the inverse relationship between UA and severity of PD was robust for men but weak for women. Our results strongly imply that either low serum UA level is a deteriorative predictor or that serum UA level serves as an indirect biomarker of prediction in PD but not in VP patients.

AgPd nanocages sandwiched between a MXene nanosheet and PDA layer for photothermally improved catalytic activity and antibacterial properties.

In this work, a MXene@AgPd/polydopamine (PDA) nanosheet with excellent photothermal conversion efficiency was successfully synthesized by a simple redox-oxidative polymerization method. Interestingly, AgPd bimetallic nanocrystals sandwiched between a MXene nanosheet and PDA layer have cage-like nanostructure, which is favorable for high catalytic efficiency and antibacterial performance. Importantly, the MXene@AgPd/PDA nanosheet exhibits good catalytic activity for the reduction of 4-nitrophenol (1.2 min-1 mg-1) and the catalytic dynamics can be improved by about 1.2 times under NIR (near-infrared light, 808 nm, and 2.5 W cm-2) irradiation. As the PDA shell is well protected, the MXene@AgPd/PDA nanosheet retained more than 90% catalytic activity after 6 cycles. In addition, due to the presence of the Ag component, the MXene@AgPd/PDA nanosheet exhibited good antibacterial activity against both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Under near-infrared light irradiation, its antibacterial activity was further enhanced due to the NIR photothermal effect.

NIR-induced improvement of catalytic activity and antibacterial performance over AuAg nanorods in Rambutan-like Fe3O4@AgAu@PDA magnetic nanospheres.

Pathogenic bacteria and difficult-to-degrade pollutants in water have been serious problems that always plague people. Therefore, finding a "one stone-two birds" method that can quickly catalyze the degradation of pollutants and show effective antibacterial behavior become an urgent requirement. This work reports a facile one-step strategy for fabricating a Rambutan-like Fe3O4@AgAu@PDA (Fe3O4@AgAu@Polydopamine) core/shell nanosphere with both catalytic and antibacterial activities which can be critically improved by externally applying an NIR laser irradiation (NIR, 808 nm) and a rotating magnetic field. Typically, the Rambutan-like Fe3O4@AgAu@PDA nanosphere have a rather rough surface due to the AuAg bimetallic nanorods sandwiched between the Fe3O4 core and the PDA shell. Owing to the penetrated PDA shell, AgAu nanorods show high and magnetically recyclable photothermal-enhanced catalytic activity for the reduction of 4-nitrophenol to 4-aminophenol and they can also be applied to initiate TMB oxidation under the help of NIR heating condition. Moreover, Fe3O4@AgAu@PDA shows a moderate antibacterial activity due to the weak release of Ag+. Under applying a rotating external magnetic field, the rough-surface Fe3O4@AgAu@PDA nanospheres produce a controllable magnetolytic force on the bacterial due to their good affinity. As a result, the Fe3O4@AgAu@PDA nanospheres show a "magnetolytic-photothermal-Ag+" synergistic antibacterial behavior against E. coli and S. aureus.

Size-dependent magnetomechanically enhanced photothermal antibacterial effect of Fe3O4@Au/PDA nanodurian.

The global health crisis of bacterial resistance to antibiotics requires innovative antibacterial strategies. One promising solution is the exploitation of multifunctional nanoplatforms based on non-resistant antibacterial mechanisms. This work reports a novel Fe3O4@Au/polydopamine (PDA) nanodurian with excellent photothermal-magnetomechanic synergistic antibacterial effects. The one-step formed Au/PDA hybrid shell provides good photothermal properties and spiky surfaces for enhanced magnetomechanic effects. Upon near-infrared (NIR) irradiation, the Fe3O4@Au/PDA nanodurian (200 µg mL-1) achieved nearly 100% antibacterial effect against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The efficiency of photothermal antimicrobial activity was further enhanced by the application of a rotating magnetic field (RMF), with the sterilization efficiency being increased by up to more than a half compared to the action alone. Interestingly, the size of the nanodurian has a significant impact on the synergistic sterilization effect, with larger particles showing a superior performance due to stronger chain-like structures in the magnetic field. Finally, the Fe3O4@Au/PDA nanodurian also demonstrates effective biofilm removal, with larger particles exhibiting the best eradication effect under the photothermal-magnetomechanic treatment. Overall, this magnetic field enhanced photothermal antibacterial strategy provides a promising broad-spectrum antimicrobial solution to combat bacterial infections. Thus, it possesses great potential in future nanomedicine and pollution treatment.

A MXene@AgAu@PDA nanoplatform loaded with AgAu nanocages for enhancing catalytic activity and antibacterial performance.

With the rapid development of social industrialization, environmental problems seriously threaten people's health, especially water pollution. Therefore, there is an urgent need to construct a multifunctional nanoplatform for different scenarios. Two-dimensional MXene@AgAu@PDA nanosheets loaded with AgAu bimetallic nanocages have been prepared by a one-step method. First, the in situ generated MXene@Ag is used as an auxiliary template, and then HAuCl4 and dopamine are added for in situ redox-oxidizing polymerization reactions to obtain AgAu catalytic nanocages and the protective polydopamine (PDA) layer which can improve the stability and biocompatibility. MXene and PDA have excellent photothermal conversion ability while hollow AgAu nanocages have strong absorption in the near-infrared region and a local surface plasmonic resonance effect. In comparison to the catalytic reaction rates under dark and room temperature conditions, the catalytic kinetic rate of MXene@AgAu@PDA nanosheets under near-infrared irradiation increases from 0.13 to 0.69 min-1 mg-1. Density functional theory (DFT) is used to study the electron transfer behavior between AgAu nanocages and MXene nanosheets, and the mechanism of the enhanced catalytic reaction rate is analyzed. Besides, due to its Ag ions and photothermal coupling antibacterial properties, 40 µg mL-1 MXene@AgAu@PDA nanosheets inactivates nearly all E. coli and S. aureus after irradiation with near-infrared light for 6 min.

High-Performance Liquid Metal/Polyborosiloxane Elastomer toward Thermally Conductive Applications.

With the combination of high flexibility and thermal property, thermally conductive elastomers have played an important role in daily life. However, traditional thermally conductive elastomers display limited stretchability and toughness, seriously restricting their further development in practical applications. Herein, a high-performance composite is fabricated by dispersing room-temperature liquid metal microdroplets (LM) into a polyborosiloxane elastomer (PBSE). Due to the unique solid-liquid coupling mechanism, the LM can deform with the PBSE matrix, achieving higher fracture strain (401%) and fracture toughness (2164 J/m2). Meanwhile, the existence of LM microdroplets improves the thermal conductivity of the composite. Interestingly, the LM/PBSE also exhibits remarkable anti-impact, adhesion capacities under complex loading environments. As a novel stretchable elastomer with enhanced mechanical and thermal behavior, the LM/PBSE shows good application prospects in the fields of thermal camouflages, stretchable heat-dissipation matrixes, and multifunctional shells for electronic devices.

Characteristics of systemic inflammation and brain iron deposition in Parkinson's disease patients.

OBJECTIVE: This study aimed at determining the characteristics of systemic inflammation and brain iron deposition in Parkinson's disease (PD) patients. METHODS: Thirty two PD patients and 30 gender- as well as age-matched controls were enrolled. Serum interleukin (IL)-1ß, IL-33, tumor necrosis factor (TNF)-α, IL-6, IL-10, ferritin, iron, and total iron binding capacity (TIBC) levels were assayed. Quantitative susceptibility mapping (QSM) was used to quantitatively analyze brain iron accumulation in the regions of interest (ROIs). Correlations between concentrations of inflammatory cytokines and biomarkers for peripheral iron metabolism, brain iron deposition were evaluated in the PD group. RESULTS: Serum concentrations of IL-1ß and IL-33 were found to be significantly elevated in the PD group compared to the control group, and in early-stage PD group compared to advanced-stage PD group. Total QSM value for bilateral ROIs was significantly elevated in the PD group compared to the control group, and in advanced-stage PD group compared to early-stage PD group. There was a significant inverse correlation between serum IL-1ß concentration and total QSM value for bilateral ROIs, between serum ferritin, iron, TIBC concentrations, and total QSM value for bilateral ROIs in PD patients. However, there was no significant correlation between serum IL-1ß concentrations and serum ferritin, iron, TIBC concentrations in PD patients. INTERPRETATION: The inflammatory state and chronic brain iron deposition progression in PD patients might be asynchronous. Alterations in systemic inflammation were not correlated with peripheral iron metabolism and might not contribute to the aggravation of brain iron deposition in PD patients.

Familial amyotrophic lateral sclerosis induced by gene mutation of SOD1G142A: a case report.

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease involving both upper and lower motor neurons. The total prevalence of ALS is [2-9]/100,000, with an annual incidence rate of 3/100,000. The disease progresses rapidly and clinically is considered to be progressive degeneration of the upper and lower motor neurons. Although this is a kind of rare disease, the mortality is high once it occurs, which has a great impact on patients and their families. Currently there is no treatment for either the sporadic or familial form. Therefore, it is of great significance to explore the diagnosis and treatment of familial amyotrophic lateral sclerosis (FALS). We report the diagnosis and treatment of a patient with familial ALS caused by mutation of the Cu/Zn superoxide dismutase (SOD1) gene c.425g > C (p.g142a), which is considered rare. We got to know that genetic testing of the patient and his immediate family members assisted in diagnosis and palliative care. Edaravone and Riluzole were used in this case according to the guideline in this case. The progress of the disease was alleviated and the survival experience of patients improved because of this medication administration. The aim of this case report is to provide a reference for the diagnosis and treatment strategy in FALS. What's more, further exploration of treatment using integrated traditional Chinese and Western medicine to delay the disease process has great significance for improved patient outcomes.

Serum antinuclear antibodies associate with worse prognosis in AQP4-positive neuromyelitis optica spectrum disorder.

BACKGROUND: Studies have demonstrated that antinuclear antibodies (ANAs) may be correlated with neuromyelitis optica spectrum disorder (NMOSD). However, the conflicting results of studies about the value of ANAs in AQP4 antibody-positive NMOSD patients need to be further investigated. MATERIAL: Case data were collected from 143 patients with AQP4 antibody-positive NMOSD. Patients were divided into two groups based on the ANA test results. The analysis of clinical characteristics, laboratory tests, and MRI examination results were compared between two groups: the NMOSD patients with ANA (+) and with ANA (-). RESULTS: Disease duration of NMOSD is shorter in the ANA (+) patients with EDSS < 4 than in the ANA (-) patients (12.05 ± 16.73 versus 29.43 ± 41.03, p-value = .013). The median time from disease onset to an EDSS score of 4.0 is significantly longer in the ANA (-) NMOSD patients than in the ANA (+) patients (48.2 months versus 24 months, p = .04). In addition, ANA (RR, 2.234; 95% CI, 1.078-4.629; p-value = .031) can predict the severity of NMOSD. CONCLUSIONS: Antinuclear antibodies seem to be associated with more severe disease activity in NMOSD patients.

Elevated Heme Oxygenase-1 Correlates With Increased Brain Iron Deposition Measured by Quantitative Susceptibility Mapping and Decreased Hemoglobin in Patients With Parkinson's Disease.

Background: Brain iron deposition, low hemoglobin (HGB), and increased heme oxygenase-1 (HO-1) have been implicated in Parkinson's disease (PD). However, the association among them in PD is poorly studied. Objective: To explore the association of the level of HO-1 with brain iron deposition and low level of HGB in PD. Methods: A total of 32 patients with PD and 26 controls were recruited for this study. C57BL/6 male mice were used in generating 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced chronic PD model. The Levels of serum HO-1 and HGB of human subjects and mice were assayed by ELISA, blood routine test, respectively. Quantitative susceptibility mapping (QSM) was used to quantitatively analyze brain iron deposition in human subjects and mice. HO-1 inhibitor (Sn-protoporphyrin, SnPP) was used to suppress the function and expression of HO-1 in PD mice. Correlations between the concentration of serum HO-1 and iron deposition of the region of interests (ROIs), levels of HGB, between the three factors mentioned above, and scores of clinical scales were explored in PD patients. Results: This study revealed significant elevation of the serum HO-1 concentration, iron deposition within bilateral substantial nigra (SN), red nucleus (RN), and putamen (PUT) and decrease of HGB level in PD patients. There was a significantly positive correlation between the serum HO-1 concentration and iron deposition within SN, an inverse correlation between the serum HO-1 concentration and HGB level in PD patients. A significant increase in HO-1 expression of serum and iron deposition in SN was also observed in the PD mouse model, and the SnPP could significantly reduce iron deposition in the SN. Conclusions: The high level of HO-1 may be the common mechanism of iron deposition and low HGB in PD. Therefore, the findings presented in this study indicate that HO-1 correlates with brain iron deposition and anemia in PD.

Near-infrared light-responsive photothermal α-Fe2O3@Au/PDA core/shell nanostructure with on-off controllable anti-bacterial effects.

Antimicrobial materials are expected to be alternatives for antibiotics against multidrug-resistant bacteria. In this paper, non-spherical α-Fe2O3@Au/PDA core/shell nanoparticles with tunable shapes are synthesized by a one-step in situ oxidation-redox polymerization method toward near infrared light-responsive antibacterial therapy. The thickness and composition of the Au/PDA hybrid shell can be controlled by varying the concentration of HAuCl4 and the dopamine precursor. Owing to the wonderful photothermal characteristics originating from the Au/PDA shell, the spindle α-Fe2O3@Au/PDA core shell nanoparticles exhibit excellent photothermal sterilization effects against both Escherichia coli and Staphylococcus aureus at low concentrations. Meanwhile, the NIR photothermal induced bactericidal performance indicates that α-Fe2O3@Au/PDA hybrid particles with tunable non-spherical shapes possess unique controllable antibacterial effects. As a result, this finding provides a simple strategy for fabricating high performance photothermal antibacterial agents and the final products possess high potential in synergistic antimicrobial therapy.

Time-Specific Pattern of Iron Deposition in Different Regions in Parkinson's Disease Measured by Quantitative Susceptibility Mapping.

Studies have shown the spatial specificity of cranial iron deposition in different regions in Parkinson's disease (PD). However, the time-specific patterns of iron deposition are not yet clear. The purpose of this study was to investigate the time pattern of iron variations and its clinical relevance in multiple gray matter nuclei in PD using quantitative susceptibility mapping (QSM). Thirty controls and 33 PD patients were enrolled, namely, 11 cases of early stage of PD (ESP) and 22 cases of advanced stage of PD (ASP) according to the Hoehn-Yahr stages. The iron content in the subcortical nuclei covering substantia nigra (SN), red nucleus (RN), head of the caudate nucleus (CN), globus pallidus (GP), and putamen (PT) was measured using QSM, and the clinical symptoms of PD were evaluated by various rating scales. The QSM values in SN, RN, GP, and PT significantly increased in PD patients compared with the controls. Further subgroup comparison with the controls indicated that the iron content in SN and GP (paleostriatum) gradually elevated in the whole disease duration and was related to clinical features. While the iron content in RN and PT (neostriatum) only elevated significantly in ESP patients, further iron deposition was not obvious in ASP patients. Our study confirmed that QSM could be used as a disease biomarker and could be suitable for longitudinal monitoring. However, considering the temporal characteristics of iron deposition in neostriatum, iron deposition in the neostriatum should be paid more attention in the early stage of the disease, even in the preclinical stage, in future research.

A refractory anti-NMDA receptor encephalitis successfully treated by bilateral salpingo-oophorectomy and intrathecal injection of methotrexate and dexamethasone: a case report.

INTRODUCTION: Anti-N-methyl-D-aspartate (anti-NMDA) receptor encephalitis is an autoimmune-mediated disease that is common in young female patients with ovarian teratomas. With appropriate immunotherapy, most patients achieve a good prognosis. Nevertheless, some patients may be refractory to first- and second-line immunotherapy, thus alternative treatments are required for these patients.Case presentation: We present a case of anti-NMDA receptor encephalitis with ovarian teratoma. After the prompt removal of the teratoma and intense immunotherapy was administered, including an intravenous methylprednisolone pulse, intravenous immunoglobin, plasmapheresis, immunoadsorption, intravenous cyclophosphamide, and rituximab, the patient's neurologic status did not improve. Bilateral salpingo-oophorectomy was then conducted, and intrathecal injection of methotrexate (MTX) and dexamethasone (DXM) was performed. The patient's neurological symptoms improved dramatically, and she achieved a good prognosis after 23 months. CONCLUSIONS: Intrathecal injection of MTX and DXM may be beneficial for treatment of refractory cases of anti-NMDA receptor encephalitis. Additional research is required to elucidate the mechanisms of intrathecal treatment with this therapy.

Atorvastatin improves motor function, anxiety and depression by NOX2-mediated autophagy and oxidative stress in MPTP-lesioned mice.

Parkinson's disease (PD) is a neurodegenerative disease caused by the loss of dopaminergic neurons. It is characterized by static tremors, stiffness, slow movements, and gait disturbances, but it is also accompanied by anxiety and depression. Our previous study showed that atorvastatin could reduce the risk of PD, but the mechanism is still unclear. In this paper, Our findings showed that atorvastatin increased muscle capacity and the coordination of movement and improved anxiety and depression. Atorvastatin could decrease the expression of α-synuclein Ser129 and NADPH oxidase 2 (NOX2), increase the protein expression of LC3II/I, and promote autophagy flow. To further confirm that atorvastatin protection was achieved by inhibiting NOX2, we injected at midbrain with NOX2 shRNA (M) lentivirus and found that silent NOX2 produced the same effect as atorvastatin. Further research found that atorvastatin could reduce MPTP-induced oxidative stress damage, while inhibiting NOX2 decreased the antioxidative stress effect of atorvastatin. Our results suggest that atorvastatin can improve muscle capacity, anxiety and depression by inhibiting NOX2, which may be related to NOX2-mediated oxidative stress and autophagy. Atorvastatin may be identified as a drug that can effectively improve behavioral disorders. NOX2 may be a potential gene target for new drug development in PD.