Comparative analysis of upward and downward vertical flow constructed wetlands on the nitrogen removal and functional microbes treating wastewater containing Ag nanoparticles.

This study investigated impacts of silver nanoparticles (AgNPs) on nitrogen removal within constructed wetlands (CWs) with different flow directions. The obtained results showed that addition of AgNPs at 0.5 and 2 mg/L significantly inhibited NH4+-N removal, resulting from lower abundances of functional genes (amoA and nxrA) within CWs. And higher abundances of amoA and nxrA genes at 0.5 mg/L were observed in downward flow CW, leading to better NH4+-N removal, compared to upward flow CW. Besides, nitrifying genes amoA and nxrA in upward flow CW at 2.0 mg/L exhibited higher than downward flow CW, explaining better NH4+-N removal in upward flow CW. 0.5 mg/L AgNPs significantly declined NO3--N and TN removal, resulted from decreasing abundances of nirK, nirS and nosZ. In contrast, abundances of nirK, nirS and nosZ genes had slightly lower or higher than before adding AgNPs in upward flow CW, leading to lower NO3--N and TN effluent concentrations. High throughput sequencing also indicated the changes of functional bacterial community after exposing to AgNPs.

Unveiling changes of microbial community involved in N and P removal in constructed wetlands with exposing to silver nanoparticles.

Constructed wetlands (CWs) are environmentally friendly engineered systems to purify wastewater, with low-cost and easy maintenance. However, it is not clear on responses of functional microbes for nitrogen (N) and phosphorus (P) biotransformation in CWs to silver nanoparticles (Ag NPs). The high throughput sequencings were employed to reveal microbial communities in vertical flow subsurface CWs with stable operation for 120 days. The results indicated that NH4+-N, TN and TP removal of soil layer decreased by 43.56%, 15.7% and 22.7% under stress of Ag NPs. Microbial richness index and compositions were affected, and control wetland enriched Sulfurospirillum, Desulfarculaceae and Flavobacterium whereas CWs exposed to Ag NPs enriched Desulfosporosinus and Desulfurispora from LEfSe analysis. Moreover, after dosing Ag NPs, relative abundances of functional genes amoA and hao for nitrification, nirK and norB for denitrification and ppx and phoA/phoD for phosphorus conversions in upper soil were significantly downregulated. Inhibition on functional bacteria and genes of Ag NPs explained poor removal efficiencies of nitrogen and phosphorus pollutants in CWs. Our findings give an insight into ecological toxicity of Ag NPs on CWs with N and P bioconversions and provide the understanding of response of nitrifiers, denitrifies and PAOs.

Hydraulic flow direction alters impacts of AgNPs on pollutant removal and silver spatial distribution in vertical flow constructed wetlands.

This study investigated the effects of AgNPs on pollutant removals in constructed wetlands (CWs) with different flow patterns and spatial distributions of silver. Before exposure to AgNPs, upward flow constructed wetland (UCW) had better nitrogen removal than down-flow CW (DCW). And 0.5 mg/L AgNPs evidently inhibited nitrogen and phosphorus removal, including ammonia, nitrate, and TP (total phosphorus), with average effluent concentrations increasing by 70.83% of NH4+-N in UCW, 18.75% of TP in UCW, and 28.33% and 25.06% of NO3--N in DCW and UCW, respectively, while COD (chemical oxygen demand) was not affected. Moreover, presence of 2 mg/L AgNPs slightly inhibited organic compounds and NH4+-N removal in two systems during stage 4 (dosing 2 mg/L AgNPs). However, the response of NO3--N and TN removal to 2 mg/L AgNPs in two systems were different, and nitrogen concentrations in effluent at the end of stage 4 significantly increased in DCW. Addition of 2 mg/L AgNPs significantly affected TP removal in two systems. Two wetlands showed high removal efficiencies of about 98% on AgNPs, indicating that CWs could provide a feasible approach for ecological restoration of nanoparticles pollution. This study also found that AgNPs mainly accumulated in the upper layer with the Ag content of 17.55-20.26 mg/kg dry weight in sand layer and 7.25-10.85 mg/kg dry weight in gravel layer. Plant roots absorbed AgNPs, with Ag content at 50.80-101.40 mg/kg and bioconcentration factors 2.80-5.00. The obtained results showed that up-flow CWs had better performance and higher resistance to the exposure of AgNPs pollution, compared with down-flow CWs.

Impacts of Ag and Ag2S nanoparticles on the nitrogen removal within vertical flow constructed wetlands treating secondary effluent.

In this study, the effects of silver (Ag NPs) and sliver sulfide nanoparticles (Ag2S NPs) on nitrogen removal and nitrogen functional microbes in constructed wetlands were investigated. The obtained results demonstrated that inhibition extent on nitrogen removal relied on NPs types and high concentrations NPs showed higher negative effects. 0.5 mg/L Ag NPs had no influence on NH4+-N removal, amoA and nxrA gene copies, whereas Ag2S NPs and Ag+ decreased NH4+-N removal by reducing abundances of nitrifying genes. The concentrations of NO3--N and TN in all 0.5 mg/L obviously increased compared with control, resulting from decreasing functional genes and denitrifying bacteria. And 0.5 mg/L Ag NPs exhibited largest inhibitory effects, with the highest NO3--N effluent concentrations. 2 mg/L Ag NPs decreased NH4+-N removal, but adverse effects gradually vanished with extension of time, whereas both Ag2S NPs and Ag+ at 2 mg/L influenced NH4+-N transformation and decreased the abundance of amoA and nxrA genes and the AOB Nitrosomonas in CWs. Moreover, 2 mg/L of Ag NPs reduced NO3--N removal by decreasing abundance of nirS and key denitrifying bacteria. To sum up, the inhibition mechanisms concluded from current results were possibly in that Ag NPs exhibited nanotoxicity rather than ionic toxicity.

Long-term effects of environmentally relevant concentration of Ag nanoparticles on the pollutant removal and spatial distribution of silver in constructed wetlands with Cyperus alternifolius and Arundo donax.

The widely usage of silver nanoparticles in a range of consumer products inevitably results in its being released to the wastewater. As a result, the potential negative effects associated with AgNPs on wastewater treatment systems need to be assessed to develop the regulatory guidelines. In this paper, the exposure experiment at environmentally relevant concentration (100 µg L-1) were conducted to demonstrate the effects of AgNPs on the pollutant removals in constructed wetlands (CWs) with different plants and the spatial distribution of silver. Before adding AgNPs, the system with Arundo donax (VF2) had the better nitrogen removal than Cyperus alternifolius (VF1). After exposure for about 94 d, the average removal efficiencies of NH4+-N significantly reduced by 32.43% and 23.92%, TN of 15.82% and 17.18% and TP of 22.74% and 20.46% in VF1 and VF2, respectively, while the COD removal had no difference. However, presence of 100 µg L-1 AgNPs for about 450 d showed no inhibition effects on nutrient removals in two experimental CWs. Two wetlands showed high removal efficiencies of about 98% on AgNPs, indicating CWs could play a crucial role to control the AgNPs release to environment. It was found that AgNPs mainly accumulated in the soil layer with the Ag content of 0.45-5.96 µg g-1 dry weight in lower soil and 2.84-11.37 µg g-1 dry weight in upper soil. The roots of Cyperus alternifolius absorbed more AgNPs, with higher bioconcentration factors (1.32-1.44) than that of 0.59 in Arundo donax. The differences of translocation factors on leaves and stems in two test plants showed that AgNPs assimilated by roots in Cyperus alternifolius were more easily transferred to the leaves. The obtained results showed that the macrophyte Cyperus alternifolius could be better choice for immobilization of AgNPs.