L-cysteine addition enhances microbial surface oxidation of coal inorganic sulfur: Complexation of cysteine and pyrite, inhibition of jarosite formation, environmental effects.

Indigenous microorganisms were used to remove inorganic sulfur from high sulfur fat coal, and effect of L-cysteine on coal surface and biodesulfurization was investigated. It was found that L-cysteine addition enhanced coal biodesulfurization, and the optimal L-cysteine dosage was 1.6 g/L. With the optimal L-cysteine dosage, the Sulfobacillus were the dominant pyrite-oxidizing bacteria. After biodesulfurization for 30 days, the inorganic sulfur in coal decreased from 3.038% to 0.437%. L-cysteine was adsorbed on the coal surface through amino, carboxyl and sulfhydryl groups, and cysteine-Fe complex was formed by the interaction between interfacial -SH group of L-cysteine and pyrite, which was beneficial to sulfur transfer. Meanwhile, L-cysteine addition improved the adsorption of microorganisms on coal surface though reducing the Zeta potential of coal particle. The structural change of coal during the biodesulfurization showed that the pyrite was solubilized by Sulfobacillus to realize the removal of inorganic sulfur from coal, and L-cysteine addition inhibited the jarosite formation through improvement of pyrite bio-oxidation and corresponding pH decrease, which avoided the dissolved sulfur returning back to coal again. Moreover, the coal biodesulfurization with L-cysteine addition also presented obvious environmental benefit.

Simultaneous in-situ remediation and fertilization of Cd-contaminated weak-alkaline farmland for wheat production.

In-situ remediation of heavy metal-contaminated farmland mainly focuses on acidic soil, however, weak-alkaline farmland widely exists in north China. Meanwhile, fertilization is usually ignored, but it may influence remediation efficiency as well as grain production. In this paper, field experiments were carried out to investigate in-situ simultaneous remediation and fertilization of Cd-contaminated weak-alkaline soil by microbial agent mixed with fulvic acid (MFA), wheat straw biochar, sepiolite and their mixture. Results showed that addition of these conditioners decreased the soil available Cd by 39.86%-71.33% and the wheat Cd by 41.94%-87.10%. The decrease order of soil available Cd followed sepiolite > mixture > biochar > MFA, while the decrease order of wheat Cd was mixture > sepiolite > biochar > MFA. With addition of mixture, the wheat Cd reduced to 0.08 mg/kg, lower than the Cd limit of 0.1 mg/kg in Contaminant Limit in Food of National Food Safety Standards (GB2762-2017), and the highest wheat yield reached 7590 kg/hm2. The MFA had significant effects on improvement of soil organic matters, nutrients and rhizosphere microbes; the biochar was prominent in improving soil organic matters, inhibiting wheat Cd and soil available Cd; the sepiolite had obvious advantages in reducing wheat Cd and soil available Cd; and the mixture had a more balanced effect on soil remediation and fertilization. Correlation study showed that soil available Cd significantly affected the uptake of Cd by wheat, and wheat yield was significantly positively correlated with soil organic matters, available N. Therefore, reducing soil available Cd, increasing soil organic matters and nutrients are the keys to simultaneous remediation and fertilization of Cd-contaminated weak-alkaline soil for wheat production.

Enhancement of biological oxygen demand detection with a microbial fuel cell using potassium permanganate as cathodic electron acceptor.

When dual-chamber microbial fuel cell (MFC) is used to detect biochemical oxygen demand (BOD), dissolved oxygen is traditionally used as cathodic electron acceptor. The detection limit of this MFC-based BOD biosensor is usually lower than 200 mg/L. In this paper, the startup of MFC-based BOD biosensor was researched and the external resistor of MFC was optimized. Results showed that the MFC started up with the dissolved oxygen as cathodic electron acceptor within 10 d, and the external resistor was optimized as 500â€¯Ω to ensure the maximum output power of MFC. Dissolved oxygen and potassium permanganate (KMnO4) were used as cathodic electron acceptor to run MFC for detection of wastewater BOD, and the performances of two kinds of BOD biosensors were compared. The MFC-based BOD biosensor using KMnO4 (10 mmol/L) as cathodic electron acceptor exhibited an excellent performance, compared with that using dissolved oxygen. The upper limit of BOD detection was greatly broadened to 500 mg/L, the response time was shortened by 50% for artificial wastewater with a BOD of 100 mg/L, and the relative error of BOD detection was reduced to less than 10%. The MFC-based BOD biosensor using KMnO4 as cathodic electron acceptor showed a better linear relationship (R2 > 0.992) between the electric charge and BOD concentration within a BOD range of 25-500 mg/L. The MFC-based BOD biosensor using the KMnO4 as cathodic electron acceptor is promising with a better application prospect.

Power Production Waste.

This paper reviewed the research literatures published in 2017 about power production waste generated from fuel and nuclear power plants. The treatment, reuse, management and disposal of power production waste were mainly considered. The wastes from different power plants mainly include fly ash, flue gas desulfurization gypsum and waste selective catalytic reduction catalyst, and so on. The monitoring, management, treatment and disposal of power production waste were also reviewed.

Thermal Effects.

This review focuses on the research literature published in 2017 relating to thermal effects in wastewater and solid waste treatment. This review is divided into the following sections: wastewater treatment, recovery of nitrogen and phosphorus, membrane technology, reduction and recovery of heavy metal, and treatment and disposal of solid waste.

Rumen fluid fermentation for enhancement of hydrolysis and acidification of grass clipping.

Rumen fluid, formed in rumen of ruminants, includes a complex microbial population of bacteria, protozoa, fungi and archaea, and has high ability to degrade lignocellulosic biomass. In this study, rumen fluid was used to ferment grass clipping for enhancing the hydrolysis and acidification of organic matters. Results showed that strict anaerobic condition, higher grass clipping content and smaller particle size of grass clipping were beneficial to the hydrolysis and acidification of organics. The increase of SCOD and total VFA concentration respectively reached 24.9 and 10.2 g/L with a suitable grass clipping content of 5%, a particle size <0.150 mm, and a fermentation time of 48 h. The VFA production was mainly attributed to the degradation of cellulose and hemicellulose with a total solid reduction of 55.7%. Firmicutes and Fibrobacteres were the major contributors to the degradation of cellulose and hemicellulose. The activity of carboxymethyl cellulose enzyme (CMCase), cellobiase and xylanase reached 0.027, 0.176 and 0.180 U/ml, respectively. The rumen fluid microorganisms successfully enhanced the hydrolysis and acidification of grass clipping.

Power Production Waste.

This review focuses on the research literature published in 2016 relating to power production waste that results from fossil fuel and nuclear power plants. It elaborates the characterization, reuse and disposal of power production wastes. The wastes from fossil fuel power plants have been divided into fly ash, flue gas desulfurization gypsum and waste catalyst. The environmental issues, associated with nuclear power plants and waste production are also reviewed.

Responses of a polycyclic aromatic hydrocarbon-degrading bacterium, Paraburkholderia fungorum JT-M8, to Cd (II) under P-limited oligotrophic conditions.

For the bioremediation of mixed-contamination sites, studies on polycyclic aromatic hydrocarbon (PAH) degradation or Cd (II) tolerance in bacteria are commonly implemented in nutrient-rich media. In contrast, in the field, inocula usually encounter harsh oligotrophic habitats. In this study, the environmental strain Paraburkholderia fungorum JT-M8 was used to explore the overlooked Cd (II) defense mechanism during PAH dissipation under P-limited oligotrophic condition. The results showed that the growth and PAH degradation ability of JT-M8 under Cd (II) stress were correlated with phosphate contents and exhibited self-regulating properties. Phosphates mainly affected the Cd (II) content in solution, while the cellular distribution of Cd (II) depended on Cd (II) levels; Cd (II) was mainly located in the cytoplasm when exposed to less Cd (II), and vice versa. The unique Cd (II) detoxification pathways could be classified into three aspects: (i) Cd (II) ionic equilibrium and dose-response effects regulated by environmental matrices (phosphate contents); (ii) bacterial physiological self-regulation, e.g., cell surface-binding, protein secretion and active transport systems; and (iii) specific adaptive responses (flagellum aggregation). This study emphasizes the importance of considering culture conditions when assessing the metal tolerance and provides new insight into the bacterial detoxification process of complex PAH-Cd (II) pollutants.

Characterization and adsorption capacity of modified 3D porous aerogel from grapefruit peels for removal of oils and organic solvents.

With the rapid industrialization, especially offshore oil exploitation, frequent leakage incidents of oils/organic solvents have adversely affected ecological systems and environmental resources. Therefore, great interest has been shown in developing new materials to eliminate these organic pollutants, which have become worldwide problems. In this study, a cost-effective, environmentally friendly porous aerogel with three-dimensional (3D) structure was prepared from grapefruit peel by a facile hydrothermal method as the adsorbent of oils/organic solvents. The as-prepared modified grapefruit peel aerogel (M-GPA) showed mesoporous structure with high specific surface area of 36.42 m2/g and large pore volume of 0.0371 cm3/g. The excellent hydrophobicity of M-GPA with a water contact angle of 141.2° indicated a strong potential for adsorption of oils and organic solvents. The high adsorption capacity of M-GPA for a series of oils and organic solvents was 8 to 52 times as much as its own weight. Moreover, the M-GPA was easily regenerated and a high adsorption capacity recovery above 97% was maintained after five adsorption-regeneration cycles. Therefore, the M-GPA is a promising recyclable adsorbent for the removal of oils/organic solvents from polluted water.

Fe1-xS/biochar combined with thiobacillus enhancing lead phytoavailability in contaminated soil: Preparation of biochar, enrichment of thiobacillus and their function on soil lead.

Properly increasing mobility of heavy metals could promote phytoremediation of contaminated soil. Fe1-xS/biochar was successfully prepared from sawdust with loading pyrrhotite (Fe1-xS) at a pyrolysis temperature of 550 °C. Thiobacillus were successfully adsorbed and enriched on the surface of Fe1-xS/biochar. Microbial growth for 36 d supported by bio-oxidization of Fe1-xS decreased the system pH from 4.32 to 3.50, increased the ORP from 298 to 487 mV, and the Fe3+ release reached 25.48 mg/g, enhancing the oxidation and leaching of soil Pb. Finally, Fe1-xS/biochar and Thiobacillus were simultaneously applied into Pb-contaminated soil for 60 d, the soil pH decreased from 7.83 to 6.72, and the exchangeable fraction of soil Pb increased from 22.86% to 37.19%. Ryegrass planting for 60 d in Pb-contaminated soil with Fe1-xS/biochar and Thiobacillus showed that the Pb content in shoot and root of ryegrass increased by 55.65% and 73.43%, respectively, confirming an obvious increase of phytoavailability of soil Pb. The relative abundance of Thiobacillus in remediated soil significantly increased from 0.06% to 34.55% due to the addition of Fe1-xS/biochar and Thiobacillus. This study provides a novel approach for regulating the Pb phytoavailability for phytoremediation of Pb-contaminated soil.

Biological nutrient removal and recovery from solid and liquid livestock manure: Recent advance and perspective.

Rapid development of livestock industry produces large amount of livestock manure rich in nutrients, organic matters, antibiotics, and heavy metals, thus imposes great harms to human and environment, if the manure is not suitably treated. Biological removal and recovery of nutrients from manure as agriculture fertilizer is attractive due to low cost and simple operation. This review offers an overview of recent development in biological nutrient removal and recovery from livestock manure. Livestock manure is divided into solid manure and liquid manure. Composting and anaerobic digestion of solid manure are fully discussed and important parameters are investigated. Then various processes of nutrient removal and recovery from liquid manure are summarized. Brief economic sustainability and eco-environmental effects are carried out. Finally, current challenges and future prospects in this field are analyzed.

Power production waste.

In this paper, the research literatures published in 2018 about power production waste generated from the coal-fired power plants and nuclear power plants are reviewed. The wastes from coal-fired plants include fly ash, flue gas desulfurization gypsum, spent selective catalytic reduction catalyst, hazardous trace elements, and the management, reuse, and disposal of these wastes are discussed. The treatment and disposal of wastes generated from nuclear power plants is also considered. PRACTITIONER POINTS: Reuse and disposal of coal-fired and nuclear power plant waste is discussed in this annual literature review. Emission of hazardous trace elements from coal-fired power plant is summarized. Radiological contaminant removal and radionuclides waste disposal is essential for nuclear power plant.

Thermal effects.

This review focuses on the research literature published in 2018 relating to thermal effects in wastewater and solid waste treatment. This review is divided into the following sections: treatment of wastewater and sludge, removal and recovery of nitrogen and phosphorus, reduction and recovery of heavy metals, membrane technology, and treatment and disposal of solid wastes. PRACTITIONER POINTS: Thermal effect plays an important role in the treatment of wastewater and sewage sludge. Recovery of nitrogen and phosphorus from wastewater and sewage sludge offers an excellent feedstock for soil amendment. Increase of treatment temperature facilitates removal and recovery of heavy metals from water and soil environment.

Contribution of solid and liquid fractions of sewage sludge pretreated by high pressure homogenization to biogas production.

High pressure homogenization (HPH) pretreatment can effectively enhance anaerobic sludge digestion. In order to understand the corresponding mechanisms, different homogenization pressures were applied on sewage sludge, and solid and liquid fractions were separately digested to clarify contribution of solid and liquid fractions to biogas production. Results showed that the methane was mainly produced from solid fraction, and methane yield was increased with the increase of pretreatment pressure. The biogas and methane production from sludge (digested without solid-liquid separation) was 17% and 45% higher than the sum of that from solid and liquid fractions (digested separately) under a pressure of 40 MPa, respectively. This indicated that the sludge liquid fraction synergistically improved the biodegradation of sludge solids. The improvement of anaerobic digestion was attributed to organic release by sludge disintegration, sludge disruption and further increase of particle surface area. The methane production was linear with effectiveness of HPH pretreatment.

Benefit of solid-liquid separation on volatile fatty acid production from grass clipping with ultrasound-calcium hydroxide pretreatment.

Ultrasound-calcium hydroxide (US-Ca(OH)2) pretreatment effectively enhanced volatile fatty acid (VFA) production from lignocellulosic biomass. In this paper, solid and liquid fraction of pretreated grass clipping was for the first time separately fermented in order to improve organic recovery from liquid fraction and reduce inhibition due to alkaline pretreatment. The total VFA yield and VS removal reached 515 mg/g TS and 59.7% after solid-liquid separation, exhibiting an increase of 116.7% and 91.9% comparing to that of mixture sample. The dominate components of VFAs are acetic and propionate acid, accounting for 80-90% of total VFAs. Kinetic analysis showed that the highest maximum VFA production rate of 690 mg/L·d and the highest cumulative VFA production potential of 3299 mg/L were achieved in the fermentation of solid fraction. Microbial analysis showed that the dominate genera for VFA production were Halocella and Ruminiclostridium, both with a relative abundance of 20.1% in fermentation of solid fraction.

Enzyme Pretreatment Enhancing Biogas Yield from Corn Stover: Feasibility, Optimization, and Mechanism Analysis.

In this study, feasibility, optimization, and mechanisms of enzyme pretreatment to enhance anaerobic digestion of corn stover were investigated. Results showed that the enzyme pretreatment efficiently enhanced the biogas yield, and the optimal conditions of enzyme pretreatment were an enzyme load of 30 FPU/g, a pretreatment time of 24 h, and a solid content of 60 g/L. Under the optimal conditions, the cumulative biogas yield increased by 36.9%, which was mainly attributed to disruption of surface structure and degradation of noncrystalline cellulose in the enzyme-pretreated corn stover. The kinetic analysis indicated that enzyme pretreatment significantly enhanced the hydrolysis rate and biogas production rate to 0.15/d and 23.89 mL/gVS, and shortened the lag phase time to 1.2 d. Correlation analysis illustrated that the SCOD yield of 250-350 mg/g from corn stover after enzyme pretreatment was suitable for the further anaerobic digestion of corn stover.