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1.
Environ Sci Technol ; 57(11): 4481-4491, 2023 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-36881938

RESUMO

The effects of sex and pregnancy on the bioaccumulation and tissue distribution of legacy and emerging per- and polyfluoroalkyl substances (PFASs) in Chinese water snakes were investigated. The bioaccumulation factor of PFASs showed a positive correlation with their protein-water partition coefficients (log KPW), and steric hindrance effects were observed when the molecular volume was > 357 Å3. PFAS levels in females were significantly lower than those in males. The chemical composition of pregnant females was significantly different from that of non-pregnant females and males. The maternal transfer efficiencies of perfluorooctane sulfonic acid were higher than those of other PFASs, and a positive correlation between the maternal transfer potential and log KPW was observed for other PFASs. Tissues with high phospholipid content exhibited higher concentrations of ∑PFASs. Numerous physiological changes occurred in maternal organ systems during pregnancy, leading to the re-distribution of chemicals among different tissues. The change in tissue distribution of PFASs that are easily and not-so-easily maternally transferred was in the opposite direction. The extent of compound transfer from the liver to the egg determined tissue re-distribution during pregnancy.


Assuntos
Ácidos Alcanossulfônicos , Fluorocarbonos , Poluentes Químicos da Água , Feminino , Gravidez , Humanos , Bioacumulação , Distribuição Tecidual , Poluentes Químicos da Água/análise , Água , Fluorocarbonos/análise , China
2.
Int J Mol Sci ; 14(3): 6187-204, 2013 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-23507754

RESUMO

A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes, designated as the PtMCP promoter, was isolated from the woody perennial Populus tomentosa. The PtMCP promoter was fused to the GUS reporter gene to characterize its expression pattern in different species. In stable Arabidopsis transformants, transcripts of the GUS reporter gene could be detected by RT-PCR in the root, stem, leaf, flower and silique. Further histochemical and fluorometric GUS activity assays demonstrated that the promoter could direct transgene expression in all tissues and organs, including roots, stems, rosette leaves, cauline leaves and flowers of seedlings and maturing plants. Its constitutive expression pattern was similar to that of the CaMV35S promoter, but the level of GUS activity was significantly lower than in CaMV35S promoter::GUS plants. We also characterized the promoter through transient expression in transgenic tobacco and observed similar expression patterns. Histochemical GUS staining and quantitative analysis detected GUS activity in all tissues and organs of tobacco, including roots, stems, leaves, flower buds and flowers, but GUS activity in PtMCP promoter::GUS plants was significantly lower than in CaMV35S promoter::GUS plants. Our results suggested that the PtMCP promoter from poplar is a constitutive promoter with moderate activity and that its function is presumably conserved in different species. Therefore, the PtMCP promoter may provide a practical choice to direct moderate level constitutive expression of transgenes and could be a valuable new tool in plant genetic engineering.

3.
Sci Total Environ ; 905: 167008, 2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-37704139

RESUMO

Laying hens were exposed to feeds spiked with a series of perfluoroalkyl carboxylates (PFCAs) ranging from perfluorobutanoic acid (C4) to perfluorooctadecanoic acid (C18) to investigate their bioaccumulation, tissue distribution, and maternal transfer. We found that PFCAs with longer carbon chains (>8) were more efficiently absorbed in the gastrointestinal tract than those with shorter chains (≤8), and that the rate of depuration varied inversely with the carbon chain length in a U-shaped pattern. Moreover, bioaccumulation potential increased with increasing carbon-chain length, except for C4. Distinct affinities were observed for specific carbon-chain PFCAs across various tissues, evident from their differential accumulation during both uptake and depuration phases. Specifically, C9 showed a higher affinity for serum and liver, C12 was more prevalent in yolk, C14 was notably abundant in the brain, and C18 was predominant in other tissues. Furthermore, the egg-maternal ratio (EMR) increased with increasing carbon-chain length from C7 to C11 and reached a plateau phase for C12 to C18. Our study also confirmed the key role of phospholipids in the tissue distribution and maternal transfer of long-chain PFCAs. This study sheds light on the interaction between PFCAs and biological tissues and reveals the toxicokinetic factors that influence the bioaccumulation of PFCAs. Further research is needed to identify the specific proteins or components that mediate the tissue-specific affinity for different carbon-chain lengths of PFCAs.


Assuntos
Galinhas , Fluorocarbonos , Animais , Feminino , Bioacumulação , Distribuição Tecidual , Galinhas/metabolismo , Fluorocarbonos/metabolismo , Ácidos Carboxílicos/metabolismo , Carbono
4.
Environ Pollut ; 246: 710-716, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30616061

RESUMO

A combination of previous studies and the present study indicated species-specific debromination of polybromodiphenyl ethers (PBDEs) in teleost fish. Three situations of debromination were found, namely rapid debromination represented by debromination of BDE 99 to BDE 47 observed in common carp, tilapia, crucian carp, and oscar fish; slow debromination represented by debromination of BDE 99 to BDE 49 observed in the abovementioned fish and rainbow trout, salmon, and snakehead; and no or minor debromination observed in catfish. The results of experiments on cofactors, inhibitors, and substrate competitors indicated that activities of outer ring deiodinase of 3, 3', 5'-triiodothyronine (type I deiodinase), which cannot be inhibited by 6-propyl-2-thiouracil, were responsible for the rapid debromination, and the outer ring deiodinase of thyroxine (type II deiodinase) regulated the slow debromination. The debromination of BDE 99 to BDE 49 was more common, but occurred at a much lower rate (approximately 100 times lower) than the debromination of BDE 99 to BDE 47. This was because the activity of type II deiodinase was nearly two orders of magnitude lower than that of type I deiodinase in the fish species studied. Further studies on debromination of PBDEs and properties of deiodinase in more species are needed to confirm the hypothesis.


Assuntos
Peixes/metabolismo , Éteres Difenil Halogenados/análise , Iodeto Peroxidase/metabolismo , Poluentes Químicos da Água/análise , Animais , Carpas/metabolismo , Peixes-Gato/metabolismo , Éteres Difenil Halogenados/metabolismo , Inativação Metabólica , Oncorhynchus mykiss/metabolismo , Salmão/metabolismo , Especificidade da Espécie , Poluentes Químicos da Água/metabolismo
5.
Environ Toxicol Chem ; 36(8): 2005-2011, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28135018

RESUMO

To explore the cause of species-specific differences and structure-activity relationships in the debromination of polybrominated diphenyl ethers (PBDEs) in fish, a series of in vitro measurements of hepatic metabolism of PBDE were made using crucian carp (Carassius carassius) and catfish (Silurus asotus) and the activity of deiodinase in liver microsomes was measured. Debromination was observed in the crucian carp but not in the catfish. No difference was found in total deiodinase activity despite the activity of type 1 deiodinase in crucian carp being twice that of catfish. It is difficult to determine whether the differences in deiodinase activity were responsible for the species-specific differences observed. In crucian carp, penta-brominated diphenyl ether congeners exhibited the highest debromination rates, and the transformation rate decreased with an increasing number of substituted bromines. Adjacent bromine substitution in the phenyl ring was a necessary, but insufficient, condition for debromination in crucian carp. Doubly flanked bromine was always preferentially removed, while single-flanked bromine, meta-substituted bromine, was debrominated the most, followed by para- and then ortho-bromine. No debromination was observed for single-flanked bromine when there was a symmetrical structure with (2, 4, 6) bromine substitutions in 1 phenyl ring, indicating that this structure can improve resistance to debromination metabolism. Environ Toxicol Chem 2017;36:2005-2011. © 2017 SETAC.


Assuntos
Carpas/metabolismo , Peixes-Gato/metabolismo , Éteres Difenil Halogenados/metabolismo , Microssomos Hepáticos/metabolismo , Bifenil Polibromatos/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Éteres Difenil Halogenados/química , Técnicas In Vitro , Iodeto Peroxidase/metabolismo , Bifenil Polibromatos/química , Especificidade da Espécie , Relação Estrutura-Atividade , Poluentes Químicos da Água/química
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