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1.
Zhongguo Zhong Yao Za Zhi ; 47(14): 3853-3862, 2022 Jul.
Artigo em Zh | MEDLINE | ID: mdl-35850844

RESUMO

This study aims to explore the potential mechanism of Liangfu Pills in the treatment of functional dyspepsia(FD) based on network pharmacology and molecular docking, and verify the mechanism by animal experiment. The active components of Liangfu Pills were screened from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), and the targets of Liangfu Pills were predicted by SwissTargetPrediction. The targets of FD were retrieved from GeneCards. On this basis, the common targets of the disease and the pills were yielded and the protein interaction was retrieved based on STRING. The core targets were screened out, followed by Gene Oncology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis with DAVID. Finally, molecular docking was carried out with the help of AutoDock Tools to predict the binding degree between the effective components of Liangfu Pills and core targets. A total of 19 active components of Liangfu Pills and 591 FD-related targets were screened out by network pharmacology, of which 253 were common targets of the disease and the prescription. Liangfu Pills was mainly involved in the biological processes of response to drug, negative regulation of transcription, positive regulation of apoptotic process, and cell surface receptor signaling pathway, and the KEGG pathways of hypoxia-inducible factor-1(HIF-1) signaling pathway, serotonergic synapse, tumor necrosis factor(TNF) signaling pathway, cyclic adenosine monophosphate(cAMP) signaling pathway, calcium signal pathway, and inflammatory mediator regulation of transient receptor potential(TRP) channels. The results of molecular docking showed that the key active components of Liangfu Pills had certain binding activity to the targets mitogen-activated protein kinase 1(MAPK1), protein kinase B(AKT1), transient receptor potential cation channel subfamily V member 1(TRPV1), 5-hydroxytryptamine receptor 1 A(HTR1 A), and 5-hydroxytryptamine receptor 2 A(HTR2 A). FD was induced in rats, and then Liangfu Pills was given to FD rats for 7 days. The results showed that Liangfu Pills could significantly relieve the symptoms of FD rats, significantly increase the expression of 5-hydroxytryptamine(5-HT), and down-regulate the expression of TRPV1. Through network pharmacology, molecular docking, and experimental verification, this study proved that Liangfu Pills improved FD through multiple components and multiple targets. The result lays a basis for further research on the mechanism and clinical application of Liangfu Pills in the treatment of FD.


Assuntos
Medicamentos de Ervas Chinesas , Dispepsia , Animais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Dispepsia/tratamento farmacológico , Medicina Tradicional Chinesa , Simulação de Acoplamento Molecular , Farmacologia em Rede , Ratos
2.
Molecules ; 22(3)2017 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-28287471

RESUMO

Four pentasaccharide resin glycosides, acutacoside F-I (1-4), were isolated from the aerial parts of Argyreia acuta. These compounds were characterized as a group of macrolactones of operculinic acid A, and their lactonization site of 11S-hydroxyhexadecanoic acid was esterified at the second saccharide moiety (Rhamnose) at C-2. The absolute configuration of the aglycone was S. Their structures were elucidated by established spectroscopic and chemical methods.


Assuntos
Glicosídeos/química , Ipomoea/química , Lactonas/química , Oligossacarídeos/química , Resinas Vegetais/química , Glicosídeos/isolamento & purificação , Lactonas/isolamento & purificação , Estrutura Molecular , Oligossacarídeos/isolamento & purificação , Ácidos Palmíticos/química , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Ramnose/química
3.
Molecules ; 20(4): 6601-10, 2015 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-25875039

RESUMO

Six pentasaccharide resin glycosides from Ipomoea cairica, including four new acylated pentasaccharide resin glycosides, namely cairicoside I-IV (1-4) and the two known compounds cairicoside A (5) and cairicoside C (6), were isolated from the aerial parts of Ipomoea cairica. Their structures were established by a combination of spectroscopic, including two dimensional (2D) NMR and chemical methods. The core of the six compounds was simonic acid A, and they were esterfied the same sites, just differing in the substituent groups. The lactonization site of the aglycone was bonded to the second saccharide moiety at C-2 in 1-4, and at C-3 in 5-6. Compounds 1 and 5, 4 and 6 were two pairs of isomers. The absolute configuration of the aglycone in 1-6 which was (11S)-hydroxyhexadecanoic acid (jalapinolic acid) was established by Mosher's method. Compounds 1-4 have been evaluated for inhibitory activity against α-glucosidase, which all showed inhibitory activities.


Assuntos
Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Ipomoea/química , Oligossacarídeos/química , Resinas Vegetais/química , alfa-Glucosidases/metabolismo , Ativação Enzimática/efeitos dos fármacos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular
4.
Zhongguo Zhong Yao Za Zhi ; 40(8): 1489-92, 2015 Apr.
Artigo em Zh | MEDLINE | ID: mdl-26281585

RESUMO

The chemical structures of P1 A was identified by complete acid hydrolysis, partial acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, IR and NMR. The results showed that P1 A had a backbone consisting rhamnose, mannose, glucose and galactose. The side chain possessed arabinose and xylose. 1-->, 1-->6 and non-reducing terminal linkages existed in polysaccharide P1A, but there are doubling amount of 1-->2 and 1-->4 linkages. Oxidable linkage of P1 A accounted for 45%, and inoxidable linkage of P1A accounted for 55%. Mannose, glucose and galactose were mainly linked by 1-->2 linkage. Rhamnose, arabinose and xylose were mainly linked by 1-->2 and 1-->4 linkages. PlA contained beta-Glc(1,6)-,beta-Gal(1,3)-,beta-Man(1,4)-beta-Rha,-Glc(1,4)-, Glc(1)-,-Gal(1,4)- and Man(1)-.


Assuntos
Acanthaceae/química , Medicamentos de Ervas Chinesas/química , Polissacarídeos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Peso Molecular
5.
Zhong Yao Cai ; 35(6): 913-7, 2012 Jun.
Artigo em Zh | MEDLINE | ID: mdl-23236827

RESUMO

OBJECTIVE: To investigate the chemical constituents from the tubers of Ipomoea batata. METHODS: The chemical constituents were isolated and purified by solvent extraction together with various chromatographic techniques. The structures were elucidated on the basis of physiochemical property and spectral data. RESULTS: 6 compounds were identified from the CHCl3 extract as Batatinoside I (1), citrusin C(2), octadecyl caffeate (3), beta-amyrin acetate (4), caffeic acid (5), scopoletin (6). CONCLUSION: Compound 1 is isolated from Ipomoea batata for the first time.


Assuntos
Alcaloides/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Ipomoea batatas/química , Lignanas/química , Lignanas/isolamento & purificação , Alcaloides/química , Ácidos Cafeicos/química , Ácidos Cafeicos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Tubérculos/química , Escopoletina/química , Escopoletina/isolamento & purificação , Espectroscopia de Luz Próxima ao Infravermelho
6.
Nat Prod Res ; 34(21): 3042-3047, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31140296

RESUMO

From the dried root and rhizomes of Atalantia buxifolia afford 11 compounds, one new compound 1 and ten known compounds 2-11 were isolated and identified. The novel compound 1 was alkaloid glycoside. Its mother nuclear was the acridone, which was relatively rare. The structure of compound 1 was established identified by spectrum and elucidated as ß-D-Glu-4,5-dimethoxy-1,6-dihydroxy-10-methyl-acridone. The compound 1 enriched the compound library and laid the material foundation for the subsequent study of pharmacological activities.


Assuntos
Alcaloides/química , Rutaceae/química , Acridonas/química , Alcaloides/isolamento & purificação , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Raízes de Plantas/química , Rizoma/química
7.
Zhong Yao Cai ; 32(8): 1232-3, 2009 Aug.
Artigo em Zh | MEDLINE | ID: mdl-19960945

RESUMO

OBJECTIVE: To study the chemical constituents of Dryopteris fragrans. METHODS: The constituents of CHCl3-soluble portion and ethyl acetate-soluble portion from the alcohol extract were isolated and purified by means of chromatography. All the compounds were identified by their physical characteristics and spectral features. RESULTS: Five compounds were isolated and identified as beta-sitosterol (I), rutin (II), quercetin (III), quercetin-3-O-beta-D-pyranglucoside (IV) and 5,7-dihydroxy-2-hydroxymethyl chromone (V). CONCLUSION: Compounds II - V are isolated from this plant for the first time.


Assuntos
Dryopteris/química , Plantas Medicinais/química , Quercetina/análogos & derivados , Rutina/isolamento & purificação , Acetatos , Etanol , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Quercetina/química , Quercetina/isolamento & purificação , Rutina/química , Sitosteroides/química , Sitosteroides/isolamento & purificação
8.
Zhong Yao Cai ; 32(8): 1285-7, 2009 Aug.
Artigo em Zh | MEDLINE | ID: mdl-19960955

RESUMO

OBJECTIVE: To investigate the anti-hypoxia active fraction of Xiangdan injection. METHODS: The anti-hypoxia experiment under normal pressure with mice was adopted on the basis of orthogonal design, and a support vector machine model was established for pharmacodynamics prediction of TCM prescriptions. Moreover, the anti-hypoxia active fraction of Xiangdan injection was determined by experimental confirmation. RESULTS: The prediction model established in this study could predict the drug actions of different formulas. CONCLUSION: As indicated in model prediction and experimental confirmation, the pharmacodynamic actions of several formulas are superior to that of the original formula, and ADE has the best effect.


Assuntos
Algoritmos , Inteligência Artificial , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Hipóxia/tratamento farmacológico , Animais , Combinação de Medicamentos , Desenho de Fármacos , Interações Medicamentosas , Medicamentos de Ervas Chinesas/administração & dosagem , Feminino , Hipóxia/mortalidade , Injeções Intraperitoneais , Masculino , Camundongos , Distribuição Aleatória , Rutaceae/química , Salvia miltiorrhiza/química , Solventes/química
9.
Zhong Yao Cai ; 31(10): 1501-3, 2008 Oct.
Artigo em Zh | MEDLINE | ID: mdl-19230398

RESUMO

OBJECTIVE: To investigate the chemical constituents of Ipomoea batatas. METHODS: Silica gel column chromatography and Sephadex LH-20 were used to separate and purify the compounds from the EtOAc and n-BuOH soluble fraction of ethanol extracts. The chemical structures were elucidated on the basis of physic-chemical properties and spectral data. RESULTS: Four compounds were isolated and identify as citrusin C (1), caffeicacid (2), 3,4-di-O-caffeoylquinic acid (3), 1,2,3,4-tetrahydro-beta-carboline-3-carboylic acid (4). CONCLUSION: Compounds 1-4 are isolated for the first time from I. batatas.


Assuntos
Alcaloides/isolamento & purificação , Ácidos Cafeicos/isolamento & purificação , Ácido Clorogênico/análogos & derivados , Ipomoea batatas/química , Plantas Medicinais/química , Alcaloides/química , Ácidos Cafeicos/química , Carbolinas/química , Carbolinas/isolamento & purificação , Ácido Clorogênico/química , Ácido Clorogênico/isolamento & purificação , Folhas de Planta/química , Espectrofotometria Ultravioleta
10.
Chin J Nat Med ; 16(5): 354-357, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29860996

RESUMO

In the present study, three compounds were isolated from Argyreia acuta, among them, compounds 1 and 2 were new and Compounds 1 and 3 were isomers. They were separated by several types of columns, such as normal phase, RP, size exclusion and preparative HPLC, and their structures were elucidated by several spectroscopic methods, such as 1D- and 2D-NMR and HR-TOF-MS.


Assuntos
Convolvulaceae/química , Glicosídeos/química , Resinas Vegetais/química , Medicamentos de Ervas Chinesas/química , Glicosídeos/isolamento & purificação , Isomerismo , Espectrometria de Massas , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Componentes Aéreos da Planta/química , Resinas Vegetais/isolamento & purificação , Espectrofotometria
11.
Nat Prod Res ; 31(5): 537-542, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27400121

RESUMO

Three new phenolic compounds, acutacoside C (1), acutacoside D (2) and acutacoside E (3) were isolated from the aerial part of Argyreia acuta. The oligosaccharide chain was composed of two glucoses and three rhamnoses, and the aglycone was (11S)-hydroxyhexadecanoic acid (jalapinolic acid). The core of the three compounds was operculinic acid B, which was rare in resin glycosides. Their structures were established by a combination of spectroscopic and chemical methods. Compounds 1-3 have been evaluated for inhibitory activity against α-glucosidase, which all showed weak inhibitory activities.


Assuntos
Convolvulaceae/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Glicosídeos/isolamento & purificação , Resinas Vegetais/isolamento & purificação , Glicosídeos/química , Glicosídeos/farmacologia , Componentes Aéreos da Planta/química , Resinas Vegetais/química , Resinas Vegetais/farmacologia
12.
Chin J Nat Med ; 14(3): 227-31, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27025370

RESUMO

In the present study, two new compounds from Ipomoea cairica were identified and demonstrated to have α-glucosidase inhibitory activity. They were isolated by column chromatography on silica gel and sephadex LH-20 and finally purified by prep-HPLC, with their structures being elucidated by spectroscopic methods, such as 1D- and 2D-NMR and HR-TOF-MS, and chemical methods. Compounds 1 and 2, named cairicoside A and cairicoside B, were evaluated for α-glucosidase inhibitory activity by the MTT method, with the IC50 values being 25.3 ± 1.6 and 28.5 ± 3.3 µmol·L(-1), respectively.


Assuntos
Inibidores de Glicosídeo Hidrolases/farmacologia , Ipomoea/química , Extratos Vegetais/farmacologia , Resinas Vegetais/farmacologia , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Estrutura Molecular , Resinas Vegetais/isolamento & purificação , Análise Espectral , alfa-Glucosidases
13.
Nat Prod Res ; 30(1): 20-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-25925631

RESUMO

Two new compounds of acutacosides 1 and 2, pentasaccharide resin glycosides were isolated from the aerial parts of Argyreia acuta. The core of the two compounds was operculinic acid A, and they were esterfied at the same position, just one substituent group was linked at C-2 of Rha. The absolute configuration of the aglycone in the two compounds was established by Mosher's method, which was (11S)-hydroxyhexadecanoic acid (jalapinolic acid). Their structures were established by a combination of spectroscopic and chemical methods.


Assuntos
Convolvulaceae/química , Oligossacarídeos/química , Resinas Vegetais/química , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Oligossacarídeos/isolamento & purificação , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Plantas Medicinais/química
14.
Food Funct ; 6(2): 479-91, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25474697

RESUMO

Strong evidence has shown that caffeine exerts antidepressant-like effects in chronic stress situations by increasing dopamine levels. However, whether caffeine mediates the dopaminergic system and interferes with the resilience to social defeat stress in mice is unknown. The aim of this study is to investigate the role of caffeine in the behavioral responses to social defeat stress and the possible regulatory role of the dopaminergic system. Mice experienced chronic social defeat stress for 10 days. Caffeine was administered intraperitoneally before, during and after social defeat stress. The time spent in interaction zone, social interaction ratio and sucrose preference test was used to measure the social avoidance and anhedonia in mice. The results showed that chronic pretreatment with caffeine for 14 days and for 10 days during stress reversed the avoidance of social behavior and anhedonia induced by social defeat stress in mice, suggesting the enhancement of the resilience to social defeat stress induced by caffeine. However, neither the treatment with caffeine only during the social defeat stress for 10 days nor the treatment with acute caffeine after defeat stress altered the resilience to stress. Furthermore, chronic caffeine treatment did not affect the normal locomotor activity and the desperate behavior in naïve mice. Moreover, the antagonism of dopamine D1 receptor and not D2 receptor reversed the effect of caffeine on the social avoidance and depressive-like behavior. Finally, pretreatment with higher doses of caffeine did not affect the behavioral response to social defeat stress. Taken together, our findings provide new insight into the effects of caffeine on social avoidance and anhedonia in mice. In addition, our results illustrated the value of measuring changes in depressive-like behavior before and after social defeat stress to determine the potential treatment of caffeine on depression through the regulation of dopaminergic system.


Assuntos
Cafeína/farmacologia , Comportamento Social , Estresse Psicológico/tratamento farmacológico , Anedonia/efeitos dos fármacos , Animais , Antidepressivos/farmacologia , Comportamento Animal/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Receptores de Dopamina D1/antagonistas & inibidores , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo
15.
Nat Prod Res ; 27(21): 1956-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23721230

RESUMO

Study of the chemical constituents of ethanol extract from the aerial parts of Atalantia buxifolia led to the identification of a new acridone alkaloid named as buxifoliadine (1), along with known compounds citrusinine--I (2), N-methylatalaphylline (3), Severinolid (4) and cycloseverinolide (5). Structural elucidation of compound 1 was carried out by a combination of mass spectrometry and (1)H and (13)C NMR spectroscopy analyses.


Assuntos
Acridinas/química , Alcaloides/química , Rutaceae/química , Acridonas/química , Etanol/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Componentes Aéreos da Planta/química , Extratos Vegetais/química
16.
J Ethnopharmacol ; 127(1): 103-7, 2010 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-19819322

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The herbal formula Xiangdan injection and its modifications have been used in traditional Chinese medicine for about hundreds years to alleviate pain and promoting blood. AIM: To investigate the anti-hypoxia active fraction of Xiangdan injection. MATERIALS AND METHODS: Xiangdan injection was extracted by ligarine, chloroform, acetic ether, n-butanol and water, represented respectively by A, B, C, D and E. Five extractions were group on the L12(2(5)) orthogonal designed table. NIH mices anti-hypoxia experiment under normal pressure with rats was adopted on the basis of orthogonal design, prediction pharmacodynamics model of TCM prescriptions was established, and the simulation bias was evaluated by combining two scatterplots. The anti-hypoxia active fraction of Xiangdan injection was determined by experiments. RESULTS: The prediction model of TCM prescriptions established in this study can predict the drug actions for different formulas, and PE%

Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Hipóxia/tratamento farmacológico , Substâncias Protetoras/química , Substâncias Protetoras/farmacologia , Animais , Fracionamento Químico , Cromatografia Líquida de Alta Pressão , Simulação por Computador , Avaliação Pré-Clínica de Medicamentos , Feminino , Hipóxia/mortalidade , Injeções Intraperitoneais , Masculino , Distribuição Aleatória , Ratos , Solventes , Fatores de Tempo
17.
Carbohydr Res ; 344(4): 466-73, 2009 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-19159869

RESUMO

Nine new lipo-oligosaccharides, batatosides H-P, were isolated from the tubers of Ipomoea batatas (Convolvulaceae). Spectral and chemical methods allowed to characterize them as tetra- or penta-saccharides that form a macrolactone with the aglycone, (11S)-hydroxyhexadecanoic acid (jalapinolic acid), the absolute configuration of which was established by Mosher's method. Batatosides L and O showed a weak inhibitory effect on the growth of Hep-2 cells, while the others proved to be inactive.


Assuntos
Ipomoea batatas/química , Lipopolissacarídeos/química , Oligossacarídeos/química , Tubérculos/química , Sequência de Carboidratos , Linhagem Celular , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular
18.
J Asian Nat Prod Res ; 10(3-4): 245-52, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18335340

RESUMO

Two new resin glycosides, batataosides I (1) and II (2), and five known compounds, friedelin (3), scopoletin (4), octadecyl caffeate (5), beta-sistosterol (6) and daucosterol (7), were isolated from the roots of Ipomoea batatas. Their structures have been determined based on the chemical and spectral data. Batataosides I and II have novel structures because the core simonic acid B was esterised with cinnamic acid for the first time, and three different substituent esterification groups in one resin glycoside is scarce. The absolute configuration of the aglycone was elucidated to be S by Mosher's method.


Assuntos
Glicosídeos/isolamento & purificação , Ipomoea batatas/química , Resinas Vegetais/isolamento & purificação , Sequência de Carboidratos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Éter/química , Glicosídeos/química , Glicosídeos/farmacologia , Humanos , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Rotação Ocular , Raízes de Plantas/química , Resinas Vegetais/química , Resinas Vegetais/farmacologia , Solubilidade , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier
19.
Chem Pharm Bull (Tokyo) ; 56(12): 1670-4, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19043237

RESUMO

Three new pentasaccharide resin glycosides, batatosides III-V (1-3), were isolated from the roots of Sweet potato (Ipomoea batatas). Saponification of the crude resin glycoside mixture yielded substituents and simonic acid B. The structures of the isolated compounds (1-3) were established through spectroscopic analyses, including high field NMR spectroscopy and HR-ESI-MS, and chemical correlation. The major characteristics of 3 are the presence of three different substituents, especially the substituent of cinnamic acid was seldom. The monosaccharides of 1-3 were proved by GC-MS and the absolute configuration of aglycone was further established as S by Mosher's method with R-methyloxyphenylacetic acid (MPA) and S-MPA.


Assuntos
Ipomoea batatas/química , Oligossacarídeos/química , Sequência de Carboidratos , Cinamatos/química , Cromatografia Gasosa-Espectrometria de Massas , Hidrólise , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Conformação Molecular , Dados de Sequência Molecular , Extratos Vegetais/química , Raízes de Plantas/química , Solubilidade , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Infravermelho
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