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1.
Small ; 20(33): e2400849, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38644168

RESUMO

Liquid organic hydrogen carrier is a promising option for the transport and storage of hydrogen as a clean energy source. This study examines the stability and behavior of organic drops immobilized on a substrate during an interfacial hydrogen-evolution reaction (HER) at the drop surface and its surrounding aqueous solution. Hydrogen microbubbles form within the drop and rise to the drop apex. The growth rate of the hydrogen in-drop bubble increases with the concentration of the reactant in the surrounding medium. The drop remains stable till the buoyancy acting on the in-drop bubble is large enough to overcome the capillary force and the external viscous drag. The bubble spontaneously rises and carries a portion drop liquid to the solution surface. These spontaneous rising in-drop bubbles are detected in measurements using a high-precision sensor placed on the upper surface of the aqueous solution, reversing the settling phase from phase separation in the reactive emulsion. The finding from this work provides new insights into the behaviors of drops and bubbles in many interfacial gas evolution reactions in clean technologies.

2.
Environ Sci Technol ; 58(35): 15619-15628, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-38272008

RESUMO

Surface-enhanced Raman spectroscopy (SERS) has been well explored as a highly effective characterization technique that is capable of chemical pollutant detection and identification at very low concentrations. Machine learning has been previously used to identify compounds based on SERS spectral data. However, utilization of SERS to quantify concentrations, with or without machine learning, has been difficult due to the spectral intensity being sensitive to confounding factors such as the substrate parameters, orientation of the analyte, and sample preparation technique. Here, we demonstrate an approach for predicting the concentration of sample pollutants from SERS spectra using machine learning. Frequency domain transform methods, including the Fourier and Walsh-Hadamard transforms, are applied to spectral data sets of three analytes (rhodamine 6G, chlorpyrifos, and triclosan), which are then used to train machine learning algorithms. Using standard machine learning models, the concentration of the sample pollutants is predicted with >80% cross-validation accuracy from raw SERS data. A cross-validation accuracy of 85% was achieved using deep learning for a moderately sized data set (∼100 spectra), and 70-80% was achieved for small data sets (∼50 spectra). Performance can be maintained within this range even when combining various sample preparation techniques and environmental media interference. Additionally, as a spectral pretreatment, the Fourier and Hadamard transforms are shown to consistently improve prediction accuracy across multiple data sets. Finally, standard models were shown to accurately identify characteristic peaks of compounds via analysis of their importance scores, further verifying their predictive value.


Assuntos
Aprendizado de Máquina , Análise Espectral Raman , Algoritmos
3.
Analyst ; 148(16): 3748-3757, 2023 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-37439271

RESUMO

Clinical semen quality assessment is critical to the treatment of infertility. Sperm DNA integrity testing provides critical information that can steer treatment and influence outcomes and offspring health. Flow cytometry is the gold standard approach to assess DNA integrity, but it is not commonly applied at the clinical level. The sperm chromatin dispersion (SCD) assay provides a simpler and cheaper alternative. However, SCD is low-throughput and non-quantitative - sperm assessment is serial, manual and suffers inter- and intra-observer variations. Here, an automated SCD analysis method is presented that enables quantitative sperm DNA quality assessment at the single-cell and population levels. Levering automated optical microscopy and a chromatin diffusion-based analysis, a sample of thousands of sperm that would otherwise require 5 hours is assessed in under 10 minutes - a clinically viable workflow. The sperm DNA diffusion coefficient (DDNA) measurement correlates (R2 = 0.96) with DNA fragmentation index (DFI) from the cytometry-based sperm chromatin structure assay (SCSA). The automated measurement of population-level sperm DNA fragmentation (% sDF) prevents inter-observer variations and shows a good agreement with the SCSA % DFI (R2 = 0.98). This automated approach standardizes and accelerates SCD-based sperm DNA analysis, enabling the clinical application of sperm DNA integrity assessment.


Assuntos
Análise do Sêmen , Sêmen , Masculino , Humanos , Análise do Sêmen/métodos , Espermatozoides , DNA/genética , DNA/análise , Cromatina/genética , Fragmentação do DNA
4.
Langmuir ; 38(37): 11227-11235, 2022 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-36067516

RESUMO

Liquid-liquid extraction based on surface nanodroplets can be a green and sustainable technique to extract and concentrate analytes from a sample flow. However, because of the extremely small volume of each droplet (<10 fL, tens of micrometers in base radius and a few or less than 1 µm in height), only a few in situ analytical techniques, such as surface-enhanced Raman spectroscopy, were applicable for the online detection and analysis based on nanodroplet extraction. To demonstrate the versatility of surface nanodroplet-based extraction, in this work, the formation of octanol surface nanodroplets and extraction were performed inside a 3 m Teflon capillary tube. After extraction, surface nanodroplets were collected by injecting air into the tube, by which the contact line of surface droplets was collected by the capillary force. As the capillary allows for the formation of ∼1012 surface nanodroplets on the capillary wall, ≥2 mL of octanol can be collected after extraction. The volume of the collected octanol was enough for the analysis of offline analytical techniques such as UV-vis, GC-MS, and others. Coupled with UV-vis, reliable extraction and detection of two common water pollutants, triclosan and chlorpyrifos, was shown by a linear relationship between the analyte concentration in the sample solution and UV-vis absorbance. Moreover, the limit of detection (LOD) as low as 2 × 10-9 M for triclosan (∼0.58 µg/L) and 3 × 10-9 M for chlorpyrifos (∼1.05 µg/L) could be achieved. The collected surface droplets were also analyzed via gas chromatography (GC) and fluorescence microscopy. Our work shows that surface nanodroplet extraction may potentially streamline the process in sample pretreatment for sensitive chemical detection and quantification by using common analytic tools.


Assuntos
Clorpirifos , Triclosan , Poluentes Químicos da Água , Poluentes da Água , Octanóis , Politetrafluoretileno , Poluentes da Água/análise , Poluentes Químicos da Água/análise
5.
Langmuir ; 37(17): 5290-5298, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33891427

RESUMO

In this work, we investigate the crystallization of droplets formed on micropatterned surfaces. By solvent exchange in a microchamber, a ternary solution consisting of a model compound ß-alanine, water, and isopropanol was displaced by a flow of isopropanol. In the process, oiling-out droplets formed and crystallized. Our results showed that the shape and size of the crystals on surfaces with chemical micropatterns could be simply mediated by the flow conditions of solvent exchange. More uniform crystals formed on hydrophilic microdomains compared to hydrophobic microdomains or homogeneous surfaces. Varying flow rates or channel heights led to the formation of thin films with microholes, connected networks of crystals, or small diamond-shaped crystals. Physical microstructures (represented by microlenses) on the surface allowed the easy detachment of crystals from the surface. Beyond oiling-out crystallization, we demonstrated that the crystal formation of another solute dissolved in the droplets could be triggered by solvent exchange. The length of crystal fibers after the solvent-exchange process was shorter at a faster flow rate. This study may provide further understanding to effectively obtain the crystallization of surface droplets through the solvent-exchange approach.

6.
Soft Matter ; 17(21): 5362-5374, 2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-33956922

RESUMO

Flow transport in confined spaces is ubiquitous in technological processes, ranging from separation and purification of pharmaceutical ingredients by microporous membranes and drug delivery in biomedical treatment to chemical and biomass conversion in catalyst-packed reactors and carbon dioxide sequestration. In this work, we suggest a distinct pathway for enhanced liquid transport in a confined space via propelling microdroplets. These microdroplets can form spontaneously from localized liquid-liquid phase separation as a ternary mixture is diluted by a diffusing poor solvent. High speed images reveal how the microdroplets grow, break up and propel rapidly along the solid surface, with a maximal velocity up to ∼160 µm s-1, in response to a sharp concentration gradient resulting from phase separation. The microdroplet propulsion induces a replenishing flow between the walls of the confined space towards the location of phase separation, which in turn drives the mixture out of equilibrium and leads to a repeating cascade of events. Our findings on the complex and rich phenomena of propelling droplets suggest an effective approach to enhanced flow motion of multicomponent liquid mixtures within confined spaces for time effective separation and smart transport processes.


Assuntos
Espaços Confinados , Preparações Farmacêuticas , Difusão , Membranas , Solventes
7.
Eur Phys J E Soft Matter ; 44(4): 57, 2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33877477

RESUMO

In situ observation of precipitation or phase separation induced by solvent addition is important in studying its dynamics. Combined with optical and fluorescence microscopy, microfluidic devices have been leveraged in studying the phase separation in various materials including biominerals, nanoparticles, and inorganic crystals. However, strong scattering from the subphases in the mixture is problematic for in situ study of phase separation with high temporal and spatial resolution. In this work, we present a quasi-2D microfluidic device combined with total internal reflection microscopy as an approach for in situ observation of phase separation. The quasi-2D microfluidic device comprises of a shallow main channel and a deep side channel. Mixing between a solution in the main channel (solution A) and another solution (solution B) in the side channel is predominantly driven by diffusion due to high fluid resistance from the shallow height of the main channel, which is confirmed using fluorescence microscopy. Moreover, relying on diffusive mixing, we can control the composition of the mixture in the main channel by tuning the composition of solution B. We demonstrate the application of our method for in situ observation of asphaltene precipitation and [Formula: see text]-alanine crystallization.


Assuntos
Dispositivos Lab-On-A-Chip , Microscopia , Difusão
8.
Environ Sci Technol ; 53(20): 11877-11887, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31525870

RESUMO

Microfibers, characterized as small fibers shed from textiles that are less than 5 mm in size, are a prominent contaminant in the environment. Thus, it is important that we have methods to accurately quantify and characterize them, including in water, sediment, wildlife, seafood, and drinking water samples. Unfortunately, their small size and the presence of different dyes on the microfibers themselves cause difficulties in identification via conventional spectroscopic methods of total attenuated resonance-Fourier transform infrared and Raman. To help solve some of these methodological challenges, we developed a new method employing polymer-dye binding chemistry, density tests, unique surface morphological traits, and fluorescent staining to identify microfibers in environmental samples. The identification method introduced here was tested in our laboratory via trials using microfibers shed from new textiles and environmental samples. We found that the method can be successfully applied to identify the different polymer types of microfibers, which can ultimately help source apportion microfiber contamination in the environment.


Assuntos
Plásticos , Poluentes Químicos da Água , Têxteis , Águas Residuárias , Água
9.
J Am Chem Soc ; 139(6): 2329-2337, 2017 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-28118008

RESUMO

In spite of the huge research interest, ionic polymers could not have been synthesized in the vapor phase because the monomers of ionic polymers contain nonvolatile ionic salts, preventing the monomers from vaporization. Here, we suggest a new, one-step synthetic pathway to form a series of cross-linked ionic polymers (CIPs) in the vapor phase via initiated chemical vapor deposition (iCVD). 2-(Dimethylamino)ethyl methacrylate (DMAEMA) and 4-vinylbenzyl chloride (VBC) monomers are introduced into the iCVD reactor in the vapor phase to form a copolymer film. Simultaneously in the course of the deposition process, the tertiary amine in DMAEMA and benzylic chloride in VBC undergo a Menshutkin nucleophilic substitution reaction to form an ionic ammonium-chloride complex, forming a highly cross-linked ionic copolymer film of p(DMAEMA-co-VBC). To the best of our knowledge, this is the first report on the synthesis of CIP films in the vapor phase. The newly developed CIP thin film is further applied to the surface modification of the membrane for oil/water separation. With the hydrophilic and underwater oleophobic membrane whose surface is modified with the CIP film, excellent separation efficiency (>99%) and unprecedentedly high permeation flux (average 2.32 × 105 L m-2 h-1) are achieved.

10.
Soft Matter ; 11(24): 4952-61, 2015 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-26008176

RESUMO

The three-dimensional (3D) clustering of Janus cylinders is controlled by simply tuning the cylinder geometry and hydrophobic interactions. Janus cylinders were prepared by combining two approaches: micromolding and initiated chemical vapor deposition (iCVD). Hydrophilic cylinders with a flat- or convex-top curvature were prepared by micromolding based on surface tension-induced flow. The iCVD process then provides a hydrophobic domain through the simple and precise deposition of a polymer film on the top surface, forming monodisperse Janus microcylinders. We use these Janus cylinders as building blocks to form 2D or 3D clusters via hydrophobic interactions in methanol. We investigate how cylinder geometry or degree of hydrophobic interaction affects the resulting cluster geometries. The convex-top Janus cylinders lead to 3D clustering through tip-to-tip interactions, and the flat-top Janus cylinders lead to 2D clustering through face-to-face attraction. The number of Janus cylinders in 3D clusters is tuned by controlling the degree of hydrophobic (or hydrophilic) interaction.

11.
Langmuir ; 30(3): 916-21, 2014 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-24393010

RESUMO

The adhesion of electrodeposited metal film to polymeric circuit board substrate is one of the key elements to successful miniaturization of electronic devices. However, as the size of the circuit pattern continuously decreases, a novel method is urgently required to increase the adhesion of the metal film on the substrate, especially on the smooth surface, which is critical to decrease the minimum feature size of the metal pattern. In this research, we developed an adhesion promoter layer by depositing metal chelating poly(4-vinylpyridine) (P4VP) film onto various organic and inorganic substrates via initiated chemical vapor deposition process (iCVD) to enhance the adhesion between the electroless deposited copper (Cu) layer and the substrate. The highest peel strength obtained between the electroless deposited Cu layer and P4VP coated substrate was 1.22 kgf/cm. Many advantageous characteristics of the adhesion promoter layer, including extreme thinness, the improved adhesion strength, conformal coverage, scalability of the deposition process, and short process time, will prompt the applicability of this adhesion promoter layer to industrial scale production.

12.
ACS Sens ; 9(3): 1489-1498, 2024 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-38440995

RESUMO

Detection of microplastics from water is crucial for various reasons, such as food safety monitoring, monitoring of the fate and transport of microplastics, and development of preventive measures for their occurrence. Currently, microplastics are detected by isolating them using filtration, separation by centrifugation, or membrane filtration, subsequently followed by analysis using well-established analytical methods, such as Raman spectroscopy. However, due to their variability in shape, color, size, and density, isolation using the conventional methods mentioned above is cumbersome and time-consuming. In this work, we show a surface-nanodroplet-decorated microfluidic device for isolation and analysis of small microplastics (diameter of 10 µm) from water. Surface nanodroplets are able to capture nearby microplastics as water flows through the microfluidic device. Using a model microplastic solution, we show that microplastics of various sizes and types can be captured and visualized by using optical and fluorescence microscopy. More importantly, as the surface nanodroplets are pinned on the microfluidic channel, the captured microplastics can also be analyzed using a Raman spectroscope, which enables both physical (i.e., size and shape) and chemical (i.e., type) characterization of microplastics at a single-particle level. The technique shown here can be used as a simple, fast, and economical detection method for small microplastics.


Assuntos
Microplásticos , Poluentes Químicos da Água , Plásticos/análise , Microfluídica , Poluentes Químicos da Água/análise , Monitoramento Ambiental , Água/análise
13.
J Colloid Interface Sci ; 643: 82-91, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37054546

RESUMO

HYPOTHESIS: Surface nanodroplets have recently been employed for in situ chemical analysis leveraging their low volume, e.g. O(10-15 L), that enables rapid analyte extraction and pre-concentration. So far, most surface nanodroplets have been formed using single organic solvents such as 1-octanol, toluene, among others. Designing multicomponent surface nanodroplet with controllable composition is highly desirable for extending their application as extractant. EXPERIMENT: Here, we formed surface nanodroplets using green deep eutectic solvent (gDES) composed of thymol and decanoic acid, both of which are naturally occurring chemicals. The influence of parameters such as flowrate and the composition of deep eutectic solvent on the surface nanodroplet formation were studied. As proof-of-concept, the gDES surface nanodroplets were further used to extract and detect trace amounts of fluorescent rhodamine 6G dye and copper ions from water. FINDINGS: The formation of gDES surface nanodroplets follows the theoretical model which states that the final droplet volume (Vf) scales with the Peclét number (Pe) of the flow during formation by the solvent exchange process, that is Vf âˆ¼ Pe3/4, and the nanodroplets demonstrate excellent ability as extractant for rhodamine 6G and copper ions from water. Surprisingly, the confined volume of gDES surface nanodroplets enables fast and controlled formation of Cu (II)-decanoate crystal.

14.
Lab Chip ; 21(13): 2574-2585, 2021 06 29.
Artigo em Inglês | MEDLINE | ID: mdl-34008650

RESUMO

A greener analytical technique for quantifying compounds in dense suspensions is needed for wastewater and environmental analysis, chemical or bio-conversion process monitoring, biomedical diagnostics, and food quality control, among others. In this work, we introduce a green, fast, one-step method called nanoextraction for extraction and detection of target analytes from sub-milliliter dense suspensions using surface nanodroplets without toxic solvents and pre-removal of the solid contents. With nanoextraction, we achieve a limit of detection (LOD) of 10-9 M for a fluorescent model analyte obtained from a particle suspension sample. The LOD is lower than that in water without particles (10-8 M), potentially due to the interaction of particles and the analyte. The high particle concentration in the suspension sample, thus, does not reduce the extraction efficiency, although the extraction process was slowed down up to 5 min. As a proof of principle, we demonstrate the nanoextraction for the quantification of model compounds in wastewater slurry containing 30 wt% solids and oily components (i.e. heavy oils). The nanoextraction and detection technology developed in this work may be used in fast analytical technologies for complex slurry samples in the environment, industrial waste, or in biomedical diagnostics.


Assuntos
Poluentes Químicos da Água , Limite de Detecção , Extração em Fase Sólida , Solventes , Suspensões , Águas Residuárias/análise , Poluentes Químicos da Água/análise
15.
Nat Rev Urol ; 18(7): 387-403, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34002070

RESUMO

Infertility rates and the number of couples seeking fertility care have increased worldwide over the past few decades. Over 2.5 million cycles of assisted reproductive technologies are being performed globally every year, but the success rate has remained at ~33%. Machine learning, an automated method of data analysis based on patterns and inference, is increasingly being deployed within the health-care sector to improve diagnostics and therapeutics. This technique is already aiding embryo selection in some fertility clinics, and has also been applied in research laboratories to improve sperm analysis and selection. Tremendous opportunities exist for machine learning to advance male fertility treatments. The fundamental challenge of sperm selection - selecting the most promising candidate from 108 gametes - presents a challenge that is uniquely well-suited to the high-throughput capabilities of machine learning algorithms paired with modern data processing capabilities.


Assuntos
Infertilidade/terapia , Aprendizado de Máquina , Injeções de Esperma Intracitoplásmicas/métodos , Motilidade dos Espermatozoides , Espermatozoides/citologia , Forma Celular , Dano ao DNA , Fertilização in vitro/métodos , Humanos , Masculino , Análise do Sêmen , Recuperação Espermática , Espermatozoides/metabolismo
16.
Lab Chip ; 21(12): 2464-2475, 2021 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-33982043

RESUMO

Sperm selection is essential for successful fertilization and embryo development. Current clinical sperm selection methods are labor-intensive and lack the selectivity required to isolate high-quality sperm. Microfluidic sperm selection approaches have shown promise but present a trade-off between the quality and quantity of selected sperm - clinicians demand both. The structure of the female reproductive tract helps to isolate a sufficient quantity of high-quality sperm for fertilization with densely folded epithelium that provides a multitude of longitudinally oriented pathways that guide sperm toward the fertilization site. Here, a three-dimensionally structured sperm selection device is presented that levers this highly parallelized in vivo mechanism for in vitro sperm selection. The device is inserted in a test tube atop 1 mL of raw semen and provides 6500 channels that isolate ∼100 000 high-DNA-integrity sperm for assisted reproduction. In side-by-side clinical testing, the developed approach outperforms the best current clinical methods by improving the DNA integrity of the selected sperm subpopulation up to 95%. Also, the device streamlines clinical workflow, reducing the time required for sperm preparation 3-fold. This single-tube, single-step sperm preparation approach promises to improve both the economics and outcomes of assisted reproduction practices, especially in cases with significant male-factors.


Assuntos
Desenvolvimento Embrionário , Espermatozoides , DNA , Feminino , Fertilização in vitro , Humanos , Masculino , Microfluídica
17.
Biotechniques ; 69(1): 404-409, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32372656

RESUMO

Poly(dimethylsiloxane) (PDMS) is widely used as a microfluidics platform material; however, it absorbs various molecules, perturbing specific chemical concentrations in microfluidic channels. We present a simple solution to prevent adsorption into a PDMS microfluidic device. We used a vapor-phase-deposited nanoadhesive layer to seal PDMS microfluidic channels. Absorption of fluorescent molecules into PDMS was efficiently prevented in the nanolayer-treated PDMS device. Importantly, when cultured in a nanolayer-treated PDMS device, yeast cells exhibited the expected concentration-dependent response to a mating pheromone, including mating-specific morphological and gene expression changes, while yeast cultured in an untreated PDMS device did not properly respond to the pheromone. Our method greatly expands microfluidic applications that require precise control of molecule concentrations.

18.
Lab Chip ; 19(5): 815-824, 2019 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-30693362

RESUMO

There is a growing appreciation and understanding of cell-to-cell variability in biological samples. However, research and clinical practice in male fertility has relied on population, or sample-based characteristics. Single-cell resolution is particularly important given the winner-takes-all nature of both natural and in vitro fertilization: it is the properties of a single cell, not the population, that are passed to the next generation. While there are a range of methods for single cell analysis, arraying a larger number of live sperm has not been possible due to the strong locomotion of the cells. Here we present a 103-trap microarray that traps, aligns and arrays individual live sperm. The method enables high-resolution imaging of the aligned cell head, the application of dye-based DNA and mitochondrial analyses, and the quantification of motility characteristics, such as tail beat. In testing, a 2400-post array trapped ∼400 sperm for individual analyses of tail beating frequency and amplitude, DNA integrity via acridine orange staining, and mitochondrial activity via staining. While literature results are mixed regarding a possible correlation between motility and DNA integrity of sperm at sample-level, results here find no statistical correlation between tail beat characteristics and DNA integrity at the cell-level. The trap array uniquely enables the high-throughput study of individual live sperm in semen samples - assessing the inherently single-cell selection process of fertilization, with single-cell resolution.


Assuntos
Separação Celular , DNA/análise , Técnicas Analíticas Microfluídicas , Imagem Óptica , Espermatozoides/química , Espermatozoides/citologia , Dimetilpolisiloxanos/química , Humanos , Masculino
19.
Food Chem ; 270: 445-451, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30174070

RESUMO

Rapid and efficient detection of pathogenic bacteria from food is critical to prevent epidemic food poisoning. However, the isolation of pathogenic bacteria from spoiled food is hampered by the lack of proper cell cultivation and/or isolation methods. Most of currently used methods suffer from complex, time-consuming culturing steps, low scalability, and high operation cost. Herein, we developed an alternative approach for the isolation of pathogenic bacteria directly from food using a surface-modified, highly porous sponge via initiated chemical vapor deposition (iCVD) process. A hydrophobic polymer, poly(2,4,6,8-tetravinyl-2,4,6,8-tetramethyl cyclotetra-siloxane) (pV4D4), was deposited conformally on amphiphilic 3-dimensional (3D) melamine sponge to incorporate hydrophobicity as well as oleophilicity to the porous sponge surface, which is appropriate for absorbing oil component selectively from food extracts. Furthermore, the surface-modified sponge was capable of the isolation of Escherichia coli O157:H7 (E. coli O157:H7) from heterogeneous mixture with oil/water/food particles with undistinguisible efficiency compare to artificial model system. The surface-modified sponge developed in this study will be a novel platform for oil/water separation and isolation of foodborne pathogens directly from heterogeneous mixture to enhance the efficiency of molecular diagnostics.


Assuntos
Contagem de Colônia Microbiana/métodos , Escherichia coli O157/isolamento & purificação , Microbiologia de Alimentos , Infecções por Escherichia coli/prevenção & controle , Doenças Transmitidas por Alimentos/prevenção & controle , Humanos , Porosidade
20.
Lab Chip ; 19(11): 1991-1999, 2019 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-31044203

RESUMO

The combination of smartphone technology and colorimetric paper-based microfluidics can enable simple, inexpensive diagnostics. However, imaging colorimetric diagnostic results via smartphones currently requires accessories to mitigate the influence of variability in surrounding lighting conditions. Here, we present an accessory-free smartphone-based colorimetric imaging method that enlists the built-in LED light source to dominate ambient lighting in combination with background and colour rescaling. This simple approach enables quantitative measurements from paper-based tests by compensating for different environmental lighting conditions and is universally applicable with respect to phone models and manufacturers. We demonstrate the method with three dominant phone makes and models in a cell counting application with a paper-based yeast detection device. The detection results are in good agreement with cell counting using automated cell counters. Eliminating the need for make/model specific accessories, this approach helps realize the potential for low-cost, broadly applicable paper-based diagnostics.


Assuntos
Colorimetria/instrumentação , Imagem Óptica/instrumentação , Papel , Cor , Dispositivos Lab-On-A-Chip , Smartphone
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