Microbial co-culture mediated by intercellular nanotubes during DMAC degradation: Microbial interaction, communication mode, and degradation mechanism.

Microbial co-culture has been proven as an effective technique for environmental remediation. In this study, co-culture mechanism of Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X during N,N-dimethylacetamide (DMAC) degradation was studied. The comparison of degradation performance in monoculture and co-culture was presented; due to the efficient cooperation between the two strains via parallel and cascaded degradation, the removal efficiency of total nitrogen (TN) in co-culture could reach 90.1%, which was 1.35 and 1.21 times higher than that of HJM-8 and YBH-X, respectively. Then the communication mode of co-culture during DMAC degradation was determined as contact-independent and contact-dependent interactions between microorganisms. Meanwhile, intercellular nanotube between HJM-8 and YBH-X was found as a unique contact-dependent interaction. The cell staining experiments and RNA sequencing analyses revealed that the nanotube could be used as a bridge to exchange cytoplasmic molecules, and thus improved material transfer and enhanced cell connection in co-culture. The results of KEGG pathway showed that differentially expressed genes in co-culture have an association with cell metabolism, nanotube generation, and genetic material transfer. Furthermore, a mechanism diagram of DMAC biodegradation was proposed for co-culture, indicating that bidirectional cooperation was established between HJM-8 and YBH-X which was mediated by the conversions of acetate and nitrogen. Finally, the co-culture system was validated for treatment of an actual wastewater; results indicated that removal efficiencies of 100% and 68.2% were achieved for DMAC and TN, respectively, suggesting that co-culture had the potential for application.

Increasing N,N-dimethylacetamide degradation and mineralization efficiency by co-culture of Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X.

In this work, Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X were isolated and used to enhance N,N-dimethylacetamide (DMAC) degradation and mineralization efficiencies. The monoculture and co-culture of the two strains for DMAC degradation were compared; results indicated that, a degradation efficiency of 97.62% was obtained in co-culture, which was much higher than that of monocultures of HJM-8 (57.34%) and YBH-X (34.02%). The degradation mechanism showed that co-culture could efficiently improve extracellular polymeric substances production, electron transfer, and microbial activity. Meanwhile, the mineralization mechanism suggested that acetate was the dominant intermediate which had an inhibitory effect on HJM-8, and co-culture was conducive to mineralization due to the high performance of acetate conversion and Na+ K+-ATPase vitality. Besides, a pathway of DMAC biodegradation was proposed for co-culture: DMAC was degraded into acetate by HJM-8, then the accumulated acetate was mineralized by YBH-X. Additionally, the co-culture system was further optimized by Box-Behnken design.

Co-existence of Alport syndrome and C3 glomerulonephritis in a proband with family history.

BACKGROUND: Alport syndrome and C3 glomerulonephritis (C3GN) are rare kidney diseases, frequently responsible for familial haematuria, proteinuria, and renal impairment. With the rapid development of molecular genetic testing, Alport syndrome causes have been restricted mostly to variants in the COL4A5 or COL4A3/COL4A4 genes. Moreover, a broad range of genetic contributors in the complement and complement-regulating proteins are definitely implicated in the pathogenesis of C3GN. METHODS: We sought a family with persistent microscopic haematuria associated with renal failure. Clinicopathologic and follow-up data were obtained, and molecular genetic testing was used to screen for pathogenic variants. RESULTS: We describe a three-generation family with Alport syndrome showing a dominant maternal inheritance. Notably, renal biopsy showed the concurrent histological evidence of C3GN in the proband harbouring an uncommon heterozygous variation in CFHR5, c.508G > A. The alteration leads to replacement of a highly conserved residue at position 170 of the ß-strand subunit of CFHR5 (p.Val170Met). In silico analysis showed that the variation was predicted to deregulate complement activation by altering the structural properties and enhancing C3b binding capacity to compete with Complement Factor H (CFH), which was in line with experimental data previously published. CONCLUSIONS: The comorbidity findings between Alport syndrome and C3GN indicate an underlying overlap and require further study.

A fungus-bacterium co-culture synergistically promoted nitrogen removal by enhancing enzyme activity and electron transfer.

The fungus Penicillium citrinum WXP-2 and the bacterium Citrobacter freundii WXP-9 were isolated and found to have poor denitrification performance. Surprisingly, co-culture of the two strains which formed fungus-bacterium pellets (FBPs) promoted the removal efficiency of nitrate (NO3--N; 95.78%) and total nitrogen (TN; 81.73%). Nitrogen balance analysis showed that excess degraded NO3--N was primarily converted to N2 (77.53%). Moreover, co-culture increased the dry weight to 0.74 g/L. The diameter of pellets and cell viability also increased by 1.49 and 1.78 times, respectively, indicating that the co-culture exerted a synergistic effect to promote growth. The increase in electron-transmission system activity [99.01 mg iodonitrotetrazolium formazan/(g·L)] and nitrate reductase activity [8.65 mg N/(min·mg protein)] were responsible for denitrification promotion. The FBPs also exhibited the highest degradation rate at 2:1 inoculation ratio and 36 h delayed inoculation of strain WXP-9. Finally, recycling experiments of FBP demonstrated that the high steady TN removal rate could be maintained for five cycles.

[Fire resistance of bark of 11 tree species]. / 11ç§æ ‘ç§çš„æ ‘çš®æŠ—ç«æ€§.

China is one of the countries with serious forest fires. Besides the methods of strengthening management of fire source and increasing forest fire monitoring and rescue efforts, improving the ability of forest itself to resist fire is also the key to prevent forest fires. In this study, we examined the characteristics of the bark across 11 main tree species in Korean pine broad-leaved forest at Jiaohe Forestry Experimental Area in Jilin Province. Fire resistance of bark acorss the tree species was evaluated comprehensively with four indices of water content, ash content, calorific value and oxygen index, using the methods of entropy weight, variance analysis and cluster analysis. Results showed that water content of bark was the highest in Phellodendron amurense. Calorific value of bark was the lowest and ash content was the highest in Ulmus davidiana. Oxygen index of bark was the highest in Fraxinus mandshurica. Bark fire resistance of 11 tree species followed the order of U. davidiana > Acer mono > Acer triflorum > Acer mandshuricum > F. mandshurica > Quercus mongolica > P. amurense > Tilia amurensis > Juglans mandshurica > Pinus koraiensis > Betula costata. The tree species in Korean pine broad-leaved forest could be classified to five categories according to bark fire resistance: U. davidiana belonged to the category of strongest fire-resistance; A. mono and A. triflorum belonged to the category of strong fire-resistance; A. mandshuricum, F. mandshurica, Q. mongolica, P. amurense, T. amurensis belonged to the category of common fire-resistance; J. mandshurica and P. koraiensis belonged to the category of weak fire-resistance; and B. costata belonged to the category of poor fire-resistance.