1.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 29(5): 425-431, 1997.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12215780
RESUMO
A fusion gene was constructed in a plasmid in which the coding regions of human GM_CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G-S-G-G-S through DNA recombinant techniques. It was then subcloned into the pBV220 expression vector, and expressed in E. coli after transformation and temperature induction. The expressed protein named as rhGM-LIF was confirmed by Western blot. After purification, the determination of activities showed that rhGM-LIF exhibited both GM-CSF and LIF activities.