The impact of non-caloric artificial sweetener aspartame on female reproductive system in mice model.

BACKGROUND: Artificial sweeteners, used as sugar substitutes have found their ways into almost all the food items due to the notion that they are non-caloric. Aspartame is used in numerous food products throughout the world. The primary users of aspartame include diabetics and calorie conscious people who intend to limit their calorie intake. METHODS: Female Swiss albino mice were divided into three groups (12 mice each) for the duration of 30 and 60 days consecutively. The treatment groups received 40 mg/kg b. w. aspartame orally. Hormone assays using ELISA and tissue histopathology have been performed along with the fertility assay to access the treatment outcomeon the fertility of treated mice in comparison to controls. RESULTS: Present study reports that female mice treated with aspartame for 30 and 60 days showed significant reduction in body weight, relative organ weight of (liver and kidney) and gonadosomatic index. These changes were more significantly recorded in 60 days treatment group. Aspartame treated animals for 30 and 60 days showed duration-dependent decrease gonandotropins (follicle stimulating hormone and luteinizing hormone), and steroids (estradiol and progesterone). Moreover, severe histopathological changes, reduction in number of growing follicles, degenerative changes in follicular structure, corona radiata and zonagranulosa were also observed. Besides, histomorphological changes were also observed in the uterine structure including atrophic uterine endometrial glands, contracted endometrial lining, disruption of the endometrial structure and the shapes of blood vessels were also altered. CONCLUSION: Non-nutritive artificial sweeteners including aspartame negatively impact the function of ovaries and feedback mechanism of reproductive hormones by affecting the hypothalamic-pituitary-gonadal axis. In light of present findings the aspartame negatively impacted the reproductive system of female mice. More studies are required to identify the molecular mechanism and the pathways involved.

Physiological Impact of the Non-Nutritive Artificial Sweetener, Aspartame, and the Therapeutic Potential of Aqueous Extract of Phyllanthus niruri.

The present study investigated the impact of aspartame (ASP) on biochemical and histological profiles and the therapeutic potential of aqueous extract of Phyllanthus niruri (PN) in female Swiss albino mice (20 ± 2 g b. w.). ASP (40 mg/kg b. w.) and PN (100 mg/kg b. w.) were fed to the mice for a period of 30 and 60 days. A significant (P ≤ .01) decrease was observed in body weight and relative organ weight in ASP-treated mice. ASP induced a significant (P ≤ .01) increase in lipid profile, bilirubin, creatinine, and enzyme activity. Furthermore, histomorphological changes in the liver and kidney, including atrophy, lesions, and derangement of cellular structure, were observed in ASP-treated animals. However, animals treated with ASP and supplemented with aqueous extract of PN showed significant (P ≤ .01) improvements in enzyme activity and histomorphological changes in the liver and kidney. Aqueous extract of PN mitigates ASP-induced physiological effects, including liver and kidney function markers and histomorphological changes. The study suggests a need for identification of mechanisms of interaction of ASP and its breakdown products once ingested and the bioactive compounds of PN responsible for its therapeutic potential.

Environmental Impact of the Presence, Distribution, and Use of Artificial Sweeteners as Emerging Sources of Pollution.

Artificial sweeteners are posing a new threat to the environment. The water ecosystem is the primary recipient of these emerging contaminants. Once ingested, sufficient amount of these artificial sweeteners escape unchanged from the human body and are added to the environment. However, some are added in the form of their breakdown products through excretion. Artificial sweeteners are resistant to wastewater treatment processes and are therefore continuously introduced into the water environments. However, the environmental behavior, fate, and long-term ecotoxicological contributions of artificial sweeteners in our water resources still remain largely unknown. Some artificial sweeteners like saccharin are used as a food additive in animal feeds. It also forms the degradation product of the sulfonylurea herbicides. All artificial sweeteners enter into the wastewater treatment plants from the industries and households. From the effluents, they finally reside into the receiving environmental bodies including wastewaters, groundwaters, and surface waters. The global production of these sweeteners is several hundred tons annually and is continuously being added into the environment.

The Perspective of Coronavirus Disease Outbreak: Epidemiology, Transmission, and Possible Treatment.

The outbreak of coronavirus disease 2019 (COVID-19) which emerged in Wuhan city of China has now spread to 214 countries and territories around the world and two international conveyances (the Diamond Princess cruise ship harbored in Yokohama, Japan and the Holland America's MS Zaandam cruise ship) as of October 11, 2020. The disease has been named as a coronavirus disease (COVID-19) and the virus causing the disease is known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). There are currently about 36,754,395 confirmed COVID-19-infected cases and 1,064,838 confirmed deaths as of October 11, 2020. The study is intended to understand the origin, transmission and impact of the coronavirus disease (COVID-19). The virus has not been encountered by humans previously and is therefore known as a novel. SARS-CoV-2 is spreading to new areas and has become a serious challenge for the scientific community in particular and the whole world population in general. The focus of the study is based on the transmission, epidemiology, genetic makeup, and possible remedies to control and contain the disease. It also includes the global impact of SARS-CoV-2 on health care and the world economy. The present review summarizes the current knowledge highlighting the symptoms, epidemiology, transmission, pathogenesis, phylogenetic analysis, and future directions to control the spread of this fatal disease.

An Overview of Ovarian Cancer: Molecular Processes Involved and Development of Target-based Chemotherapeutics.

Ovarian cancer is one of the leading gynecologic diseases with a high mortality rate worldwide. Current statistical studies on cancer reveal that over the past two decades, the fifth most common cause of death related to cancer in females of the western world is ovarian cancer. In spite of significant strides made in genomics, proteomics and radiomics, there has been little progress in transitioning these research advances into effective clinical administration of ovarian cancer. Consequently, researchers have diverted their attention to finding various molecular processes involved in the development of this cancer and how these processes can be exploited to develop potential chemotherapeutics to treat this cancer. The present review gives an overview of these studies which may update the researchers on where we stand and where to go further. The unfortunate situation with ovarian cancer that still exists is that most patients with it do not show any symptoms until the disease has moved to an advanced stage. Undoubtedly, several targets-based drugs have been developed to treat it, but drug-resistance and the recurrence of this disease are still a problem. For the development of potential chemotherapeutics for ovarian cancer, however, some theoretical approaches have also been applied. A description of such methods and their success in this direction is also covered in this review.

Potential biomarkers of emotional stress induced neurodegeneration.

Mental health is a matter of great significance and interest both socially and scientifically. The present review aims to provide an informative platform about the classical biomarkers available to identify and diagnose the neurodegeneration induced by emotional stress and depression. Present article provides an expert comprehensive overview of the universally accepted markers and their mechanism of action involved in emotional stress assessment and its management. This envisioned piece of work emphasize on the incorporation of clinical markers in classical psychiatry experiments will make the information more significant, reliable and universally accepted. The information summaries in the article will facilitates the researchers of clinical psychiatry, neuropharmacology and neuropsychiatry in management of depressive disorders along with the identification of possible neurodegenerative association.

Effect of cold stress on infanticide by female Swiss albino mice Mus musculus: a pilot study.

BACKGROUND: Mice are widely accepted research models of great clinical significance. Maintenance of laboratory mice breed is an essential aspect for performing research activities in various fields of science. Infanticide is one of the prominent causes of litter loss during maintenance of laboratory mice stock. The present study is an effort to monitor the effect of change in ambient temperature of female mice below the normal range on cannibalism and infanticide during early postparturition phase. Adult female Swiss albino mice have been divided into two groups of control and treatment. On the day of litter group one was maintained under controlled temperature conditions (minimum 20 °C to maximum 23 °C) throughout, while female mice belong to group two have been exposed to variation of room temperature (maximum 15 °C to minimum 10 °C for two nights and one day) until 36 h postparturition. RESULTS: The effects of temperature changes were observed on the infanticide behaviour of dams along with the survival of pups in early postparturition phase till 36 h after delivery. The significant statistical difference (P < 0.05) was reported in infanticide behaviour of dams when control and treatment group was compared. It is observed that decrement in surrounding temperature promotes decrement in the ambient body temperature of dams during early postparturition. It is proposed that alteration of hypothalamic homeostasis due to temperature change induces cannibalism and infanticide behaviour. Lack of thermoregulation during early postparturition creates the sense of insecurity, in-satiety, anxiety and stress. CONCLUSIONS: Authors strongly recommend the maintenance of body and surrounding temperature to prevent infanticidal behaviour and cannibalism within Swiss albino mice population. Further investigations are advisable to authenticate the active behavioural and biochemical pathway behind the phenomena.

Health Implications Associated with Aspartame Consumption: A Substantial Review.

Aspartame, an artificial sweetening agent belongs to dipeptide chemical category with a very strong sweetening potential. Although research findings in humans and non-human primates have demonstrated numerous negative effects of aspartame (biochemical, histological, neurological, behavioral, genetic etc)., the status of aspartame is still debatable. Present manuscript is a critical review of the substantial research findings related to aspartame intake on different research models. Purpose of this review was to spread the awareness about adverse effect of aspartame intake to outline the occurrence of health issues among the population. The process of uptake, storage, compartmentalization and distribution of aspartame within the body is associated with metabolic disorders and various clinical conditions. Available research literature indicates that higher amount of aspartame ingestion should be monitored carefully to avoid health implication within society.

Estimation of Anti-HIV Activity of HEPT Analogues Using MLR, ANN, and SVM Techniques.

The present study deals with the estimation of the anti-HIV activity (log1/C) of a large set of 107 HEPT analogues using molecular descriptors which are responsible for the anti-HIV activity. The study has been undertaken by three techniques MLR, ANN, and SVM. The MLR model fits the train set with R (2)=0.856 while in ANN and SVM with higher values of R (2) = 0.850, 0.874, respectively. SVM model shows improvement to estimate the anti-HIV activity of trained data, while in test set ANN have higher R (2) value than those of MLR and SVM techniques. R m (2) = metrics and ridge regression analysis indicated that the proposed four-variable model MATS5e, RDF080u, T(O⋯O), and MATS5m as correlating descriptors is the best for estimating the anti-HIV activity (log 1/C) present set of compounds.