RESUMO
The indirect enzyme-linked immunosorbent assay (ELISA) was applied to detect antibody to infectious bronchitis (IB) virus in chickens. A cross-reactivity study was performed to determine the antigenic relationships among six strains: Massachusetts, Connecticut, Beaudette, and three Ontario field isolates. For comparison, the cross-reactivity study was performed in eggs using the serum-neutralization (SN) test for IB. The ELISA proved to be more broadly cross-reactive than the IB-SN test. This suggests that the ELISA for IB will be useful as a rapid diagnostic test for serum profiling in unvaccinated flocks, regardless of the IB serotype involved.
Assuntos
Anticorpos Antivirais/análise , Antígenos Virais/imunologia , Coronaviridae/imunologia , Vírus da Bronquite Infecciosa/imunologia , Animais , Galinhas , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Vírus da Bronquite Infecciosa/classificação , Testes de Neutralização , SorotipagemRESUMO
The indirect enzyme-linked immunosorbent assay (ELISA) was used to detect antibody to infectious bronchitis virus in chickens. The serum-neutralization test for infectious bronchitis (SNIB) was used as a reference serologic test. The ELISA proved to be useful for monitoring antibody responses following vaccination of leghorn chicks. The titers obtained with the ELISA and SNIB showed an overall correlation, but results suggest the involvement of different antibody classes.
Assuntos
Anticorpos Antivirais/análise , Galinhas/imunologia , Infecções por Coronaviridae/veterinária , Coronaviridae/imunologia , Vírus da Bronquite Infecciosa/imunologia , Doenças das Aves Domésticas/imunologia , Animais , Infecções por Coronaviridae/diagnóstico , Infecções por Coronaviridae/imunologia , Ensaio de Imunoadsorção Enzimática , Doenças das Aves Domésticas/diagnóstico , Testes SorológicosRESUMO
Necrotizing pancreatitis was observed in 2-week-old Guinea fowl submitted for necropsy and histopathology. Intranuclear inclusion bodies seen histologically in acinar epithelium were examined by electron microscopy and found to contain viruses resembling adenovirus. Adenovirus was isolated in embryonated eggs from the pancreata of affected birds. The adenovirus isolated was not neutralized by chicken antisera developed against 10 known serotypes of group 1 avian adenoviruses.
Assuntos
Infecções por Adenoviridae/veterinária , Adenoviridae/isolamento & purificação , Aviadenovirus/isolamento & purificação , Pancreatite/veterinária , Doenças das Aves Domésticas/microbiologia , Infecções por Adenoviridae/microbiologia , Infecções por Adenoviridae/patologia , Animais , Corpos de Inclusão Viral/microbiologia , Corpos de Inclusão Viral/ultraestrutura , Microscopia Eletrônica , Pancreatite/microbiologia , Pancreatite/patologia , Aves Domésticas/microbiologia , Doenças das Aves Domésticas/patologia , Organismos Livres de Patógenos EspecíficosRESUMO
The method(s) of spread of infectious laryngotracheitis in poultry flocks is uncertain. Based on an outbreak of the disease in the Niagara Peninsula in the summer of 1982, managers of infected (case) flocks and noninfected (control) flocks were interviewed to compare their management practices. The data were statistically analyzed; however, the results are not conclusive since only thirteen farms were involved in the outbreak. The results do suggest the following: farms with previous infectious laryngotracheitis infected flocks are more likely to have future outbreaks than are infectious laryngotracheitis-free farms, sanitation procedures of people entering the barns (changing boots and coveralls) appears to be related to infection status and air inlet location on the barn may be associated with infection. These items should be examined more thoroughly in future outbreaks.