PuHSFA4a Enhances Tolerance To Excess Zinc by Regulating Reactive Oxygen Species Production and Root Development in Populus.

Zinc (Zn) is an essential micronutrient but in excess is highly toxic to plants. Plants regulate Zn homeostasis and withstand excess Zn through various pathways; these pathways are generally tightly regulated by a specific set of genes. However, the transcription factors involved in excess Zn tolerance have yet to be identified. Here, we characterized a Populus ussuriensis heat shock transcription factor A4a (PuHSFA4a) that acts as a positive regulator of excess Zn tolerance in P ussuriensis We used overexpression (PuHSFA4a-OE) and chimeric dominant repressor (PuHSFA4a-SRDX) lines to identify the targets of PuHSFA4a PuHSFA4a transcription is specifically induced in roots by high Zn. Overexpression of PuHSFA4a conferred excess Zn tolerance and a dominant repressor version of PuHSFA4a increased excess Zn sensitivity in P ussuriensis by regulating the antioxidant system in roots. PuHSFA4a coordinately activates genes related to abiotic stress responses and root development and directly binds to the promoter regions of glutathione-s-transferase U17 (PuGSTU17) and phospholipase A2 (PuPLA2 ). PuGSTU17 overexpression significantly increased GST activity and reduced reactive oxygen species levels in roots while PuGSTU17-RNA interference lines exhibited the opposite phenotype. Furthermore, PuPLA2 overexpression promoted root growth under high Zn stress. Taken together, we provide evidence that PuHSFA4a coordinately activates the antioxidant system and root development-related genes and directly targets PuGSTU17 and PuPLA, thereby promoting excess Zn tolerance in P ussuriensis roots.

Performance of underwater quantum key distribution with polarization encoding.

Underwater quantum key distribution (QKD) has potential applications in absolutely secure underwater communication. However, the performance of underwater QKD is limited by the optical elements, background light, and dark counts of the detector. In this paper, we propose a modified formula for the quantum bit error rate (QBER), which takes into account the effect of detector efficiency on the QBER caused by the background light. Then we calculate the QBER of the polarization encoding BB84 protocol in Jerlov-type seawater by analyzing the effect of the background light and optical components in a more realistic situation. Finally, we further analyze the final key rate and the maximum secure communication distance in three propagation modes, i.e., upward, downward, and horizontal modes. We find that secure QKD can be performed in the clearest Jerlov-type seawater at a distance of hundreds of meters, even in the worst downward propagation mode. Specifically, by optimizing the system parameters, it is possible to securely transmit information with a rate of 67 kbits/s at a distance of 100 m in the seawater channel with an attenuation coefficient of 0.03/m at night. For practical underwater QKD, the performance can also be improved by using decoy states. Our results are useful for long-distance underwater quantum communication.

Experimental investigation of quantum key distribution over a water channel.

Quantum key distribution (QKD) has undergone significant development in recent decades, particularly with respect to free-space (air) and optical fiber channels. Here, we report a proof-of-principle experiment for the BB84 protocol QKD over a water channel. First, we demonstrate again the polarization preservation properties of the water channel in optical transmission according to the measured Mueller matrix, which is close to the unit matrix. The reason for the polarization preservation, revealed by Monte Carlo simulation, is that almost all the received photons are unscattered. Then, we performed the first polarization encoding BB84 protocol QKD over a 2.37 m water channel. The results show that QKD can be performed with a low quantum bit error rate, less than 3.5%, with different attenuation coefficients.

Channel analysis for single photon underwater free space quantum key distribution.

We investigate the optical absorption and scattering properties of underwater media pertinent to our underwater free space quantum key distribution (QKD) channel model. With the vector radiative transfer theory and Monte Carlo method, we obtain the attenuation of photons, the fidelity of the scattered photons, the quantum bit error rate, and the sifted key generation rate of underwater quantum communication. It can be observed from our simulations that the most secure single photon underwater free space QKD is feasible in the clearest ocean water.

Accumulation of kaempferitrin and expression of phenyl-propanoid biosynthetic genes in kenaf (Hibiscus cannabinus).

Kenaf (Hibiscus cannabinus) is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL) was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H) and 4-coumarate-CoA ligase (Hc4CL) were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS), chalcone isomerase (HcCHI), and flavone 3-hydroxylase (HcF3H) was highest in young flowers, whereas that of flavone synthase (HcFLS) was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold) in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

Riboflavin accumulation and molecular characterization of cDNAs encoding bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase, lumazine synthase, and riboflavin synthase in different organs of Lycium chinense plant.

Riboflavin (vitamin B2) is the precursor of flavin mononucleotide and flavin adenine dinucleotide-essential cofactors for a wide variety of enzymes involving in numerous metabolic processes. In this study, a partial-length cDNA encoding bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase (LcRIBA), 2 full-length cDNAs encoding lumazine synthase (LcLS1 and LcLS2), and a full-length cDNA encoding riboflavin synthase (LcRS) were isolated from Lycium chinense, an important traditional medicinal plant. Sequence analyses showed that these genes exhibited high identities with their orthologous genes as well as having the same common features related to plant riboflavin biosynthetic genes. LcRIBA, like other plant RIBAs, contained a DHBPS region in its N terminus and a GCHII region in its C-terminal part. LcLSs and LcRS carried an N-terminal extension found in plant riboflavin biosynthetic genes unlike the orthologous microbial genes. Quantitative real-time polymerase chain reaction analysis showed that 4 riboflavin biosynthetic genes were constitutively expressed in all organs examined of L. chinense plants with the highest expression levels found in the leaves or red fruits. LcRIBA, which catalyzes 2 initial reactions in riboflavin biosynthetic pathway, was the highest transcript in the leaves, and hence, the richest content of riboflavin was detected in this organ. Our study might provide the basis for investigating the contribution of riboflavin in diverse biological activities of L. chinense and may facilitate the metabolic engineering of vitamin B2 in crop plants.

Molecular characterization of carotenoid biosynthetic genes and carotenoid accumulation in Lycium chinense.

Lycium chinense is a shrub that has health benefits and is used as a source of medicines in Asia. In this study, a full-length cDNA clone encoding ß-ring carotene hydroxylase (LcCHXB) and partial-length cDNA clones encoding phytoene synthase (LcPSY), phytoene desaturase (LcPDS), ξ-carotene desaturase (LcZDS), lycopene ß-cyclase (LcLCYB), lycopene ε-cyclase (LcLCYE), ε-ring carotene hydroxylase (LcCHXE), zeaxanthin epoxidase (LcZEP), carotenoid cleavage dioxygenase (LcCCD1), and 9-cis epoxycarotenoid dioxygenase (LcNCED) were identified in L. chinense. The transcripts were constitutively expressed at high levels in leaves, flowers and red fruits, where the carotenoids are mostly distributed. In contrast, most of the carotenoid biosynthetic genes were weakly expressed in the roots and stems, which contained only small amounts of carotenoids. The level of LcLCYE transcripts was very high in leaves and correlated with the abundance of lutein in this plant tissue. During maturation, the levels of lutein and zeaxanthin in L. chinense fruits dramatically increased, concomitant with a rise in the level of ß-cryptoxanthin. LcPSY, LcPDS, LcZDS, LcLCYB, and LcCHXE were highly expressed in red fruits, leading to their substantially higher total carotenoid content compared to that in green fruits. Total carotenoid content was high in both the leaves and red fruits of L. chinense. Our findings on the biosynthesis of carotenoids in L. chinense provide insights into the molecular mechanisms involved in carotenoid biosynthesis and may facilitate the optimization of carotenoid production in L. chinense.

Benefits and trade-offs of replacing inorganic fertilizer by organic substrate in crop production: A global meta-analysis.

Replacing inorganic fertilizer with organic substrate contributes to sustainable agricultural production capacity. However, the effects of organic substitution regimes (OSR) on global crop productivity, soil carbon (C) and nitrogen (N) losses and biofertility as function of environmental variables have not been systematically quantified. Here, we have conducted a meta-analysis of these effects using field data (211 papers with 852 observations) collected around the world. Results indicated that OSR increased crop productivity (3.04 %) and soil biofertility (soil qMBC, qMBN, microbial richness, Shannon and functionality by 11.4 %, 21.1 %, 10.2 %, 3.95 %, and 38.5 %, respectively), and reduced soil N losses (N2O emissions, NH3 volatilization and soil N leaching by 26.5 %, 26.1 %, and 33.8 %, respectively), but increased CO2 emissions (19.4 %), and paddy fields CH4 emissions (41.2 %). N rate was an important factor influencing crop productivity and soil biofertility response to OSR, and crop productivity and soil biofertility had a greater positive response at moderate substitution rates in acid soil and long-term trials, but full substitution significantly decreased crop yield. Furthermore, the increase in soil biofertility and crop yield saturated in ~10-14 and ~ 22 years after organic substrate input. The emissions of CO2, CH4, and N2O significantly increased with increasing substitution rates, while the opposite was true for N leaching. The NH3 volatilization response to OSR presented a positive effect in acidic and coarse texture soil. OSR was more beneficial in mitigating soil C and N loss response (except CO2 emissions) in uplands compared to paddy fields. Therefore, implementation of OSR requires site-specific strategies to better achieve a balance between increasing crop production and reducing environmental benefits. Given that the OSR improvement varies depending on environmental variables, we propose a predictive model to initially assess the potential for OSR improvement. This study will provide scientific guidance on the reasonable application of organic substrate in agroecosystems.

High-Frequency Hearing Loss, Hippocampal Volume, and Motoric Cognitive Risk Syndrome in Older Adults in China: A Population-Based Study.

Background: Little is known about the associations of hearing loss, hippocampal volume, and motoric cognitive risk syndrome (MCR) in older adults. Objective: We aimed to investigate the associations of hearing loss with MCR and hippocampal volume; and the interaction of hearing loss with hippocampal volume on MCR. Methods: This population-based cross-sectional study included 2,540 dementia-free participants (age≥60 years; 56.5% women) in the baseline examination of the Multimodal Interventions to Delay Dementia and Disability in rural China. Data were collected through face-to-face interviews, clinical examination, and laboratory tests. Hearing function was assessed using pure tone audiometry test. In the subsample (n = 661), hippocampal volume was assessed on structural magnetic resonance images. Data were analyzed with logistic regression models. Results: In the total sample, MCR was diagnosed in 246 persons (9.7%). High-frequency hearing loss was significantly associated with an increased likelihood of MCR and slow gait. In the subsample, the restricted cubic spline plots indicated an inverted U-shaped nonlinear relationship between high-frequency hearing performance and hippocampal volume. Moreover, greater hippocampal volume was significantly associated with a deduced likelihood of MCR and subjective cognitive decline (SCD). In addition, there were statistical interactions of high-frequency hearing loss with hippocampal volume on MCR and slow gait (p for interaction < 0.05), such that the associations were statistically significant only among participants free of high-frequency hearing loss. Conclusions: High-frequency hearing loss was associated with an increased likelihood of MCR in older adults. The hippocampus might play a part in the relationship of high-frequency hearing loss and MCR.

Identification of phenylpropanoid biosynthetic genes and phenylpropanoid accumulation by transcriptome analysis of Lycium chinense.

BACKGROUND: Lycium chinense is well known in traditional Chinese herbal medicine for its medicinal value and composition, which have been widely studied for decades. However, further research on Lycium chinense is limited due to the lack of transcriptome and genomic information. RESULTS: The transcriptome of L. chinense was constructed by using an Illumina HiSeq 2000 sequencing platform. All 56,526 unigenes with an average length of 611 nt and an N50 equaling 848 nt were generated from 58,192,350 total raw reads after filtering and assembly. Unigenes were assembled by BLAST similarity searches and annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. Using these transcriptome data, the majority of genes that are associated with phenylpropanoid biosynthesis in L. chinense were identified. In addition, phenylpropanoid biosynthesis-related gene expression and compound content in different organs were analyzed. We found that most phenylpropanoid genes were highly expressed in the red fruits, leaves, and flowers. An important phenylpropanoid, chlorogenic acid, was also found to be extremely abundant in leaves. CONCLUSIONS: Using Illumina sequencing technology, we have identified the function of novel homologous genes that regulate metabolic pathways in Lycium chinense.

Large-scale plantlet conversion and ex vitro transplantation efficiency of Siberian ginseng by bioreactor culture.

To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of torpedo-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets had a higher root number and leaf number and larger leaf area than did HS3 (culture of heart-shaped SEs in a bioreactor with a 3-week subculture interval) and HS2 (culture of heart-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets. Of these converted plants, TS2 plantlets had higher survival rate (83.7%) and growth characteristics after transplantation in a simple shed covered with a 50% sunshade net only for 6 months. TS2 plantlets also showed significantly lower H2O2 content and significantly increased superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione transferase (GST) expression levels as compared to HS2 plants when exposure to ex vitro conditions.

Balancing potato yield, soil nutrient supply, and nitrous oxide emissions: An analysis of nitrogen application trade-offs.

Potato has been promoted as a national key staple food to alleviate pressure on food security in China. Appropriate nitrogen (N) application rate is prerequisite and is crucial for increasing yield, improving fertilizer efficiency, and reducing N losses. In the present study, we determined the optimum N application rates by analyzing field trial data from the main potato producing areas of China between 2004 and 2020. We considered the equilibrium relationships between potato yield, N uptake, partial N balance (PNB), and N2O emission under different soil indigenous N supply (INS) scenarios. The results showed that N rate, INS, and their interactions all significantly affect potato yield and nutrient uptake increment. On average, N application increased potato yield and N uptake by 29.5 % and 56.7 %, respectively. The relationship between N rate and yield increment was linear-plateau, while the relationship between N rate and N uptake increment was linear-linear. Soil INS accounted for 63.5 % of total potato N requirement. Potato yield increment and nutrient uptake increment were exponentially negatively correlated with INS and had a significant parabolic-nonlinear relationship with the interaction of N fertilizer application rate and INS. PNB was negatively correlated with fertilizer N supply intensity as a power function. Based on our analysis, a N application rate of 166 kg N ha-1 was found to be sufficient when the target yield was <34 t ha-1. However, when the target yield reached 40, 50 and 60 t ha-1, the recommended N application rate increased to 182, 211, and 254 kg N ha-1, respectively, while ensuring N2O emissions low with an emission factor of 0.2 %. Our findings will help guide potato farming toward cleaner production without compromising environmental benefit.

Selective adsorption and separation of methylene blue by facily preparable xanthan gum/amantadine composites.

In this work, xanthan gum-based composites were successfully graft-modified by amantadine (XG-Fe3+/AM) with higher adsorption capacity and selectivity on recycling cationic dye (methylene blue, MB) from aqueous solution. The adsorption equilibrium of MB could be achieved approximately within 5 min when the initial concentration was 100 mg/L, and the maximum adsorption capacity was up to 565 mg/g. After 5 desorption-regeneration cycles, the removal rate of XG-Fe3+/AM for MB could still be as high as 95 % with slight decrement. Additionally, the effects of pH, contact time, temperature and initial dye concentration on the adsorption performance of MB were systematically examined. Furthermore, the adsorbent was characterized by FT-IR, BET and XPS analysis. In mixed anionic and cationic dyes, the adsorption selectivity of XG-Fe3+/AM on MB in the mixture of MB and methyl orange (MO) reached up to 99.69 %. Molecular dynamics simulation revealed that the trend of adsorption energy for dyes was in good agreement of the experimental order of adsorption capacities and molecular sizes among seven anionic and cationic dyes based on molecular matching effect and electrostatic interaction. Therefore, XG-Fe3+/AM is an eco-friendly, facile-synthesis and high-selectivity adsorbent, which remove cationic dyes in multi-component systems through electrostatic interaction and molecular matching effect.

Long-term combined organic manure and chemical fertilizer application enhances aggregate-associated C and N storage in an agricultural Udalfs soil.

Little information is known on whether carbon (C) and nitrogen (N) immobilization is synchronized in different sizes of aggregates under different agricultural management practices. Carbon and N concentrations and the C/N ratios in different sizes of aggregates down to 40 cm depth were determined after long-term application of chemical fertilizers combined with manure or without manure in a wheat-rice cropping system. Manure application usually produced significantly (P < 0.05) higher C and N concentrations and lower C/N ratios in bulk soil and in different sizes of aggregates down to 20 cm depth than the other treatments, and the 1.5 MNPK treatment at 0-10 cm depth had the highest SOC concentration of 26.3 g/kg. The C and N concentrations in bulk soil and all aggregate fractions decreased markedly with increasing soil depth. Among water stable aggregates in all soil depths, the highest C (48.2-66.4%) and N (47.8-68.3%) concentrations as a percentage of SOC were found in the small macroaggregates (2000-250 µm, SM). Manure application significantly (P < 0.05) increased the mass and C and N concentrations of SM and SM fractions down to 20 depth. The mean C/N ratios of silt-clay within large and small macroaggregates (inter-SC) were 1.57 and 1.46 units lower than those of silt-clay particles, respectively, indicating that inter-SC had relative high N availability. Moreover, the C and N content of SM down to 40 cm depth tended to saturation with increasing C input rate. Overall, manure application effectively improved soil structure, SM were the dominant particles involved in soil C and N storage, and inter-SC were the main particles responsible for N availability.

Characteristics of rice straw decomposition and bacterial community succession for 2 consecutive years in a paddy field in southeastern China.

The characteristics of long-term rice straw decomposition and succession in the bacterial community in the double-rice system are still unclear. Here a 2-year continuous straw bag decomposition experiment was conducted to explore changes in nutrient release, enzyme activity, and bacterial community composition during rice straw decomposition in the double-rice system in Southeast China. After burial in soil, the cumulative dry matter loss rates of rice straw were 38.9%, 72.6%, and 82.7% after 2, 12, and 24 months, respectively. The change in the release rate of straw nitrogen and phosphorus was similar to the dry matter loss, but 93.5% of straw potassium was released after 1st month. Bacterial abundance and community diversity in straw increased rapidly, reaching peaks after 7 and 12 months, respectively. Straw extracellular enzyme activities were the highest in the first 2 months and then gradually decreased over time, and they significantly and positively correlated with straw decomposition rate. Straw decomposition was dominated by copiotrophic Bacilli and Flavobacteriia in the early stages and evolved to be dominated by oligotrophic Acidobacteria, Anaerolineae, Deltaproteobacteria, Saccharibacteria, and Sphingobacteriia in the later stages. Changes in the C/N and K content of straw are the main reasons for bacterial community succession during rice straw decomposition. This study can provide a scientific basis for developing efficient decomposing bacteria agents for rice straw.

Differences in total stored C and N in dryland red soil caused by different long-term fertilization practices.

Fertilizer application is important to achieve sustainable agriculture. However, it remains unclear about the effects of long term fertilization on C and N immobilization as well as C/N ratios in soil aggregates at different depths. Samples taken at depths of 0 to 40 cm from dryland red soil subjected to long-term fertilization were analyzed. Four treatments were involved in the long term fertilization including no fertilizer (control), chemical fertilizer applied at two different rates, and manure combined with chemical fertilizers (MNPK). The C and N concentrations in the soil aggregates of different sizes were significantly higher (P < 0.05) and the C/N ratios in the particulate organic matter were significantly lower (P < 0.05) for soil to 20 cm deep for the MNPK treatment than for the other treatments. ANOVA indicated that the C and N concentration and C/N ratios in different sizes of aggregates significantly varied with soil depth (P < 0.05). Microaggregates contained most of the C and N, and the C/N ratios for silt-clay particles in macroaggregates were 1.37 unit (ranging - 0.25 to 2.44) lower than for other soil particles with diameters < 53 µm. The C and N contents in aggregates of different sizes increased as the C input rate increased to a depth of 40 cm because of the fertilization practices. Overall, both increased C input and deep application of C sources promoted the storage of C and N in microaggregates, which in turn increased C and N sequestration in dryland red soils.

Effects of chicken manure substitution for mineral nitrogen fertilizer on crop yield and soil fertility in a reduced nitrogen input regime of North-Central China.

Organic manure has been proposed to substitute part of the chemical fertilizers. However, past research was usually conducted in regimes with excessive nitrogen (N) fertilization, which was not conducive to the current national goal of green and sustainable development. Therefore, exploring the potential of organic fertilizer substitution for mineral N fertilizer under regimes with reduced N inputs is important to further utilize organic fertilizer resources and establish sustainable nutrient management recommendations in the winter wheat (Triticum aestivum L.) - summer maize (Zea mays L.) rotation system in North-central China. In this study, a 4-year field experiment was conducted to investigate the effects of different chicken manure substitution ratios on crop yield, N recovery efficiency (REN), soil N and soil organic matter contents, to clarify the optimal organic substitution ratio of N fertilizer under reduced N application (from 540 kg N ha-1 year-1 to 400 kg N ha-1 year-1). Six substitution ratios were assessed: 0%, 20%, 40%, 60%, 80% and 100% under 200 kg N ha-1 per crop season, respectively, plus a control with no N application from chemical fertilizer or chicken manure. Results showed that the highest yield was achieved under the 20% substitution ratio treatment, with 1.1% and 2.3% higher yield than chemical N alone in wheat season and maize seasons, respectively. At the chicken manure substitution ratios of 20% in wheat season and 20%-40% in maize season, the highest REN reached to 31.2% and 26.1%, respectively. Chicken manure application reduced soil residual inorganic N with increasing substitution ratio. All organic substitution treatments increased soil organic matter and total N content. Implementing 20% organic substitution in wheat season and 20%-40% in maize season under the reduced N application regime in the North-central China is therefore recommended in order to achieve high crop yields and REN, improve soil fertility and enhance livestock manure resource utilization.

Characterization of the early gene expression profile in Populus ussuriensis under cold stress using PacBio SMRT sequencing integrated with RNA-seq reads.

Populus ussuriensis is an important and fast-growing afforestation plant species in north-eastern China. The whole-genome sequencing of P. ussuriensis has not been completed. Also, the transcriptional network of P. ussuriensis response to cold stress remains unknown. To unravel the early response of P. ussuriensis to chilling (3 °C) stress and freezing (-3 °C) stresses at the transcriptional level, we performed single-molecule real-time (SMRT) and Illumina RNA sequencing for P. ussuriensis. The SMRT long-read isoform sequencing led to the identification of 29,243,277 subreads and 575,481 circular consensus sequencing reads. Approximately 50,910 high-quality isoforms were generated, and 2272 simple sequence repeats and 8086 long non-coding RNAs were identified. The Ca2+ content and abscisic acid (ABA) content in P. ussuriensis were significantly increased under cold stresses, while the value in the freezing stress treatment group was significantly higher than the chilling stress treatment group. A total of 49 genes that are involved in the signal transduction pathways related to perception and transmission of cold stress signals, such as the Ca2+ signaling pathway, ABA signaling pathway and MAPK signaling cascade, were found to be differentially expressed. In addition, 158 transcription factors from 21 different families, such as MYB, WRKY and AP2/ERF, were differentially expressed during chilling and freezing treatments. Moreover, the measurement of physiological indicators and bioinformatics observations demonstrated the altered expression pattern of genes involved in reactive oxygen species balance and the sugar metabolism pathway during chilling and freezing stresses. This is the first report of the early responses of P. ussuriensis to cold stress, which lays the foundation for future studies on the regulatory mechanisms in cold-stress response. In addition the full-length reference transcriptome of P. ussuriensis deciphered could be used in future studies on P. ussuriensis.

Efficiently ion-enhanced adsorption of anion dyes by acrolein crosslinked polyethylenimine/chitosan hydrogel with excellent recycling stability.

A novel adsorbent acrolein crosslinked polyethylenimine/chitosan hydrogel (A-PEI/CS) was developed with excellent recycling stability and ion-enhanced effect on removing anionic dye (acid blue 93, AB93) from aquatic environment. For AB93, A-PEI/CS was such an adsorbent with the characteristics of high adsorption capacity up to 1212.4 mg/g and continuous recyle ability of more than 15 times. After 15 desorption-regeneration cycles, the removal rate of A-PEI/CS for AB93 could still be as high as 96 % with imperceptibly downward trend. In addition, the addition of salts (KCl, NaCl and CaCl2) could promote the adsorption of A-PEI/CS, and the removal rate would be enhanced with increasing concentration of the salt. The effects of pH (2-7), contact time (30-600 min), temperature (30-50 °C) on the adsorption performance of A-PEI/CS were systematically examined. Furthermore, the physicochemical properties of the adsorbent were analyzed by FT-IR, XPS and zeta potential. The adsorption mechanism can be explained by electrostatic interaction, hydrogen bond interaction, chemical interaction. Intriguing to note that, the adsorption energy of adsorbents was investigated by molecular simulation, and the low pH and common salt environment can promote the adsorption effect, indicating that the prepared adsorbent has excellent application value in the treatment of practical high-salinity wastewater.

[Study on the chemical constituents of Codonopsis lanceolata].

OBJECTIVE: To study the chemical constituents of Codonopsis lanceolata. METHODS: Chemical constituents were separated with the column chromatographic, and their structures were identified by chemical and spectroscopic methods. RESULTS: Six compounds were isolated and identified as syringin (1), shikimic acid (2), friedelin (3), alpha-spinasterol (4), stigmasterol (5), stigmasta-7-dien-3beta-ol (6). CONCLUSION: Compounds 3-6 are isolated from this plant for the first time.