Land use and urbanization indirectly control riverine CH4 and CO2 emissions by altering nutrient input.

Urban rivers are recognized as significant sources of methane (CH4) and carbon dioxide (CO2) emissions. Despite this, the influence of land use and urbanization on carbon emissions across rural-urban rivers at the watershed scale has been insufficiently explored. This study utilized in-situ surveys of the Liao River in northern China to investigate the spatial and temporal variations of CH4 and CO2 emissions and their relationship with urbanization and its potential controlling factors. The findings revealed that CH4 emissions peaked in fall, whereas CO2 emissions were highest in summer. The average fluxes of CH4 and CO2 at the water-gas interface were 1387.22 ± 2474.98 µmol·m-2·d-1 and 52.78 ± 54.44 mmol·m-2·d-1, respectively. Water quality parameters accounted for 80.49 % of the total variation in CH4 and CO2 concentrations and fluxes. Structural equation modeling indicated that TN, TP, DTC, and conductivity had direct effects on riverine CH4 and CO2 emissions, with standardized direct effects of 0.50 and 0.49, respectively. Nutrient input emerged as the primary driver, increasing CH4 and CO2 concentrations and fluxes, particularly in urban-adjacent river sections likely receiving higher nutrient loads. This study underscores that land use and urbanization indirectly influence riverine CH4 and CO2 emissions by modifying nutrient inputs. Effective land use management and nutrient input control are recommended strategies to mitigate riverine CH4 and CO2 emissions.

Narcolepsy and risk of traumatic injury: a population-based matched cohort study.

STUDY OBJECTIVES: We evaluated the risk of traumatic injury in patients with narcolepsy compared to the general population. METHODS: We conducted a population-based matched cohort study using a Japanese health insurance claims database. For each patient with narcolepsy, up to 5 individuals from the general population without narcolepsy were matched by variables such as sex, age, and cohort entry month. The primary outcome was traumatic injury, and the secondary outcome was fracture. The study population was followed for up to 5 years from the cohort entry date. We estimated crude incidence rates, adjusted incidence rate differences (aIRDs), adjusted hazard ratios (aHRs), and their 95% confidence intervals (CIs) for study outcomes using crude and multivariable Poisson and Cox regression models. RESULTS: We included 2,451 patients with narcolepsy (mean age, 30.3 years; male, 58.0%) and 10,591 matched individuals (mean age, 30.6 years; male, 58.4%). Crude incidence rate of traumatic injury was 11.4 per 100 person-years for patients with narcolepsy compared with 6.2 per 100 person-years for matched individuals (aIRD, 6.2 excess events per 100 person-years [95% CI, 4.9-7.4]; aHR, 1.8 [95% CI, 1.5-2.2]). Crude incidence rate of fracture was 2.3 per 100 person-years for patients with narcolepsy compared with 1.3 per 100 person-years for matched individuals (aIRD, 1.2 excess events per 100 person-years [95% CI, 0.7-1.7]; aHR, 1.7 [95% CI, 1.4-2.1]). CONCLUSIONS: Narcolepsy was associated with increased risk of traumatic injury. For patients with narcolepsy, optimized approaches to injury prevention should be considered.

Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification.

Co-immobilization of cells and enzymes is often essential for the cascade biocatalytic processes of industrial-scale feasibility but remains a vast challenge. Herein, we create a facile co-immobilization platform integrating enzymes and cells in covalent organic frameworks (COFs) to realize the highly efficient cascade of inulinase and E. coli for bioconversion of natural products. Enzymes can be uniformly immobilized in the COF armor, which coats on the cell surface to produce cascade biocatalysts with high efficiency, stability and recyclability. Furthermore, this one-pot in situ synthesis process facilitates a gram-scale fabrication of enzyme-cell biocatalysts, which can generate a continuous-flow device conversing inulin to D-allulose, achieving space-time yield of 161.28 g L-1 d-1 and high stability (remaining >90% initial catalytic efficiency after 7 days of continuous reaction). The created platform is applied for various cells (e.g., E. coli, Yeast) and enzymes, demonstrating excellent universality. This study paves a pathway to break the bottleneck of extra- and intracellular catalysis, creates a high-performance and customizable platform for enzyme-cell cascade biomanufacturing, and expands the scope of biocatalysis process intensification.