Molecular characterization of classical and nonclassical MHC class I genes from the golden pheasant (Chrysolophus pictus).

Classical major histocompatibility complex (MHC) class I allelic polymorphism is essential for competent antigen presentation. To improve the genotyping efforts in the golden pheasant, it is necessary to differentiate more accurately between classical and nonclassical class I molecules. In our study, all MHC class I genes were isolated from one golden pheasant based on two overlapping PCR amplifications. In total, six full-length class I nucleotide sequences (A-F) were identified, and four were novel. Two (A and C) belonged to the IA1 gene, two (B and D) were alleles derived from the IA2 gene through transgene amplification, and two (E and F) comprised a third novel locus, IA3 that was excluded from the core region of the golden pheasant MHC-B. IA1 and IA2 exhibited the broad expression profiles characteristic of classical loci, while IA3 showed no expression in multiple tissues and was therefore defined as a nonclassical gene. Phylogenetic analysis indicated that the three IA genes in the golden pheasant share a much closer evolutionary relationship than the corresponding sequences in other galliform species. This observation was consistent with high sequence similarity among them, which likely arises from the homogenizing effect of recombination. Our careful distinction between the classical and nonclassical MHC class I genes in the golden pheasant lays the foundation for developing locus-specific genotyping and establishing a good molecular marker system of classical MHC I loci.

Effects of gender and age on thixotropic properties of whole blood from healthy adult subjects.

The thixotropic parameters of whole blood from 314 healthy subjects (154 women, 160 men) were measured with our modified method by Low shear 30 Rheometer and calculated according Huang's equation. This communication offered the reference range of thixotropic parameters from man and woman group. The results demonstrated that no significant differences existed in the plasma viscosity and fibrinogen between man and woman group. Man group had statistically higher values in HCT, yield stress (tau 0), Newtonian contribution of viscosity (mu), non-Newtonian contribution of viscosity (eta s--mu), apparent viscosity at 2.37 sec-1 (eta s), the equilibrium value of the structural parameter (A) and apparent kinetic rate constant of rouleaux breakdown (ARC) than those in woman group. The man and woman groups could be separately divided into five subgroups in terms of age. It was found that the levels of fibrinogen and plasma viscosity had a tendency of increasing with aging. In the old subgroup (greater than 60 years) of men and women HCT, tau v, mu, eta s, (eta s--mu) and A had significant lower values than those in young and middle-age subgroups. However, it was very interested that there were differences of ARC versus age between man group and woman group, i.e. ARC in the man subgroup II, IV had lower and the woman subgroup II, III, IV had higher values than their respective older subgroup did.

[Research on thixotropic parameters of whole blood from Chinese adult subjects].

This study measured the thixotropic parameters of whole blood from 314 healthy subjects (154 women, 160 men) with our modified method by Low Shear-30 Rheometer according to Huang's equation. We suggested the range of normal values of thixotropic parameters from man and woman group. The results demonstrated that no significant differences existed in the plasma viscosity (eta p) and fibrinogen between man and woman group. Man group had significantly higher values than those in woman group in hematocrit (HCT), yield stress (tau 0), Newtonian contribution of viscosity (mu), non-Newtonion contribution of viscosity (eta s-mu), apparent viscosity at 2.37s-1 (eta s), the equilibrium value of the structural parameter (A) and apparent kinetic rate constant of rouleaux breakdown (ARC). The man and woman groups could be separately divided into five subgroups on the basis of age. All parameters were compared with one another and treated statistically. We found that the levels of fibrinogen and eta p had an increasing trend with aging. In the old subgroup (greater than or equal to 60 years) of man and woman, HCT, tau 0, mu, eta s, (eta s-mu) and A had significantly lower values than those in young and middle age subgroups. However, it was very interesting that there were differences of ARC versus age between the man and woman groups, i.e., ARC in the man subgroup 3, 4 had lower values and in the woman subgroup 2, 3, 4 had higher values than their respective older subgroups did.