RESUMO
AIM: To investigate the antiepileptic effect of dizocilpine (MK-801) on amygdala kindling models in rats and the effects of its combination with general antiepileptic drugs. METHODS: To establish amygdala kindling models in rats and observe the effect of dizocilpine on kindling models and its combination with general antiepileptic drugs (phenobarbital, valproate and nicardipine) at ineffective dose. The influence of dizocilpine on convulsions induced by semicarbazide (SCZ) in mice were also observed. RESULTS: Dizocilpine (0.1-0.25 mg.kg-1, i.p.) was shown to dose-dependently inhibit amygdala kindled seizure, shorten the after discharge duration (ADD) and reduce the Racine's stage (P < 0.01). The combination of dizocilpine with phenobarbital, valproate, nicardipine at ineffective dose shortened ADD or reduced Racine's stages (P < 0.01). Dizocilpine (0.1-0.25 mg.kg-1, i.p.) significantly prolonged the latency and reduced the rate of convulsions and death in mice. CONCLUSION: Dizocilpine inhibits the seizure of the amygdala kindling and improve the antiepileptic activity of phenobarbital, valproate and nicardipine, indicating that these combination may provide a new approach for treating epilepsy.
Assuntos
Tonsila do Cerebelo/fisiopatologia , Anticonvulsivantes/farmacologia , Maleato de Dizocilpina/farmacologia , Epilepsia/tratamento farmacológico , Excitação Neurológica/efeitos dos fármacos , Tonsila do Cerebelo/efeitos dos fármacos , Animais , Anticonvulsivantes/uso terapêutico , Maleato de Dizocilpina/uso terapêutico , Estimulação Elétrica , Epilepsia/induzido quimicamente , Feminino , Masculino , Camundongos , Nicardipino/farmacologia , Fenobarbital/farmacologia , Distribuição Aleatória , Ratos , Ratos Wistar , Semicarbazidas , Ácido Valproico/farmacologiaRESUMO
AIM: To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A. METHODS: Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. The ultra-structure of the skin was observed through electronic microscope. RESULTS: PCF could enhance the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and total anti-oxidative capacity (T-AOC). Also PCF could reduce the amount of MDA, increase the expression of Bcl-2 protein, and inhibit the expression of NOS protein. The ultra-structure of epidermis and fibroblasts remained normal in 20 % PCF groups; there were vacuoles in smooth endoplasm reticulum in epidermis of mice and the number of rough endoplasm reticulum in fibroblasts was decreased in model group. CONCLUSION: PCF had the protective effects on hairless mice skin damaged by ultraviolet A via its anti-oxidative mechanisms.