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C4 plants are expected to have faster stomatal movements than C3 species because they tend to have smaller guard cells. However, little is known about how the evolution of C4 photosynthesis influences stomatal dynamics in relation to guard cell size and environmental factors. We studied photosynthetically diverse populations of the grass Alloteropsis semialata, showing that the origin of C4 photosynthesis in this species was associated with a shortening of stomatal guard and subsidiary cells. However, for a given cell size, C4 and C3-C4 intermediate individuals had similar or slower light-induced stomatal opening speeds than C3 individuals. Conversely, when exposed to decreasing light, stomata in C4 plants closed as fast as those in non-C4 plants. Polyploid formation in some C4 plants led to larger stomatal cells and was associated with slower stomatal opening. Conversely, diversification of C4 diploid plants into wetter environments was associated with an acceleration of stomatal opening. Overall, there was significant relationship between light-saturated photosynthesis and stomatal opening speed in the C4 plants, implying that photosynthetic energy production was limiting for stomatal opening. Stomatal dynamics in this wild grass therefore arise from the evolving interplay between photosynthetic physiology and the size and biochemical function of stomatal complexes.
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BACKGROUND: The Porcine Epidemic Diarrhea Virus (PEDV) has caused significant economic losses in the global swine industry. As a potential drug for treating diarrhea, the antiviral properties of attapulgite deserve further study. METHODS: In this study, various methods such as RT-qPCR, Western blot, viral titer assay, Cytopathic Effect, immunofluorescence analysis and transmission electron microscopy were used to detect the antiviral activity of attapulgite and to assess its inhibitory effect on PEDV. RESULTS: When exposed to the same amount of virus, there was a significant decrease in the expression of the S protein, resulting in a viral titer reduction from 10-5.613 TCID50/mL to 10-2.90 TCID50/mL, which represents a decrease of approximately 102.6 folds. Results of cytopathic effect and indirect immunofluorescence also indicate a notable decrease in viral infectivity after attapulgite treatment. Additionally, it was observed that modified materials after acidification had weaker antiviral efficacy compared to powdered samples that underwent ultrasonic disintegration, which showed the strongest antiviral effects. CONCLUSION: As a result, Attapulgite powders can trap and adsorb viruses to inhibit PEDV in vitro, leading to loss of viral infectivity. This study provides new materials for the development of novel disinfectants and antiviral additives.
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Antivirais , Vírus da Diarreia Epidêmica Suína , Compostos de Silício , Vírus da Diarreia Epidêmica Suína/efeitos dos fármacos , Vírus da Diarreia Epidêmica Suína/genética , Vírus da Diarreia Epidêmica Suína/fisiologia , Animais , Antivirais/farmacologia , Compostos de Silício/farmacologia , Compostos de Silício/química , Chlorocebus aethiops , Compostos de Magnésio/farmacologia , Suínos , Células Vero , Carga Viral/efeitos dos fármacos , Efeito Citopatogênico Viral/efeitos dos fármacos , Doenças dos Suínos/virologia , Infecções por Coronavirus/virologia , Infecções por Coronavirus/veterinária , Microscopia Eletrônica de TransmissãoRESUMO
AIM: To discover the populations of mesenchymal stem cells (MSCs) derived from different layers of human maxillary sinus membrane (hMSM) and evaluate their osteogenic capability. MATERIALS AND METHODS: hMSM was isolated into a monolayer using the combined method of physical separation and enzymatic digestion. The localization of MSCs in hMSM was performed by immunohistological staining and other techniques. Lamina propria layer-derived MSCs (LMSCs) and periosteum layer-derived MSCs (PMSCs) from hMSM were expanded using the explant cell culture method and identified by multilineage differentiation assays, colony formation assay, flow cytometry and so on. The biological characteristics of LMSCs and PMSCs were compared using RNA sequencing, reverse transcription and quantitative polymerase chain reaction, immunofluorescence staining, transwell assay, western blotting and so forth. RESULTS: LMSCs and PMSCs from hMSMs were both CD73-, CD90- and CD105-positive, and CD34-, CD45- and HLA-DR-negative. LMSCs and PMSCs were identified as CD171+/CD90+ and CD171-/CD90+, respectively. LMSCs displayed stronger proliferation capability than PMSCs, and PMSCs presented stronger osteogenic differentiation capability than LMSCs. Moreover, PMSCs could recruit and promote osteogenic differentiation of LMSCs. CONCLUSIONS: This study identified and isolated two different types of MSCs from hMSMs. Both MSCs served as good potential candidates for bone regeneration.
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Diferenciação Celular , Seio Maxilar , Células-Tronco Mesenquimais , Osteogênese , Humanos , Células-Tronco Mesenquimais/citologia , Osteogênese/fisiologia , Seio Maxilar/citologia , Citometria de Fluxo , Proliferação de Células , Células Cultivadas , Separação Celular/métodos , Masculino , Adulto , Feminino , Periósteo/citologiaRESUMO
Strontium-containing agents have been demonstrated to elicit both bone anabolic and antiosteoporotic effects, showing great potential for the treatment of bone loss. However, an increased incidence of strontium-induced side effects restricts their clinical applications. Herein, oxidized carbon nitride nanosheets (CN) are delicately used to incorporate Sr2+ for the first time to achieve high osteogenic efficacy. The lamellar structure and enriched nitrogen species of CN provide them with a high surface area-to-volume ratio and abundant anchoring sites for Sr2+ incorporation. Importantly, Sr2+-incorporated CN (CNS) could synergistically promote osteoblast differentiation and bone regeneration at a single, very low Sr2+ dose. Mechanically, CNS could activate the FAK/RhoA signaling pathway to modulate the intracellular tension that stimulates osteoblasts differentiation. The present study will provide a new paradigm to enhance the efficacy of osteogenic metal ions by using lamellar nanocarriers.
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Regeneração Óssea , Estrôncio , Estrôncio/farmacologia , Osteogênese , Osso e OssosRESUMO
Physiological microenvironment engineering has shown great promise in combating a variety of diseases. Herein, we present the rational design of reinforced and injectable blood-derived protein hydrogels (PDA@SiO2-PRF) composed of platelet-rich fibrin (PRF), polydopamine (PDA), and SiO2 nanofibers that can act as dual-level regulators to engineer the microenvironment for personalized bone regeneration with high efficacy. From the biophysical level, PDA@SiO2-PRF with high stiffness can withstand the external loading and maintaining the space for bone regeneration in bone defects. Particularly, the reinforced structure of PDA@SiO2-PRF provides bone extracellular matrix (ECM)-like functions to stimulate osteoblast differentiation via Yes-associated protein (YAP) signaling pathway. From the biochemical level, the PDA component in PDA@SiO2-PRF hinders the fast degradation of PRF to release autologous growth factors in a sustained manner, providing sustained osteogenesis capacity. Overall, the present study offers a dual-level strategy for personalized bone regeneration by engineering the biophysiochemical microenvironment to realize enhanced osteogenesis efficacy.
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Hidrogéis , Fibrina Rica em Plaquetas , Regeneração Óssea , Osteogênese , Fibrina Rica em Plaquetas/metabolismo , Dióxido de Silício/metabolismoRESUMO
The repair of severe bone defects is still a formidable clinical challenge, requiring the implantation of bone grafts or bone substitute materials. The development of three-dimensional (3D) bioprinting has received considerable attention in bone tissue engineering over the past decade. However, 3D printing has a limitation. It only takes into account the original form of the printed scaffold, which is inanimate and static, and is not suitable for dynamic organisms. With the emergence of stimuli-responsive materials, four-dimensional (4D) printing has become the next-generation solution for biological tissue engineering. It combines the concept of time with three-dimensional printing. Over time, 4D-printed scaffolds change their appearance or function in response to environmental stimuli (physical, chemical, and biological). In conclusion, 4D printing is the change of the fourth dimension (time) in 3D printing, which provides unprecedented potential for bone tissue repair. In this review, we will discuss the latest research on shape memory materials and 4D printing in bone tissue repair.
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Bioimpressão , Materiais Inteligentes , Engenharia Tecidual/métodos , Osso e Ossos , Bioengenharia , Impressão Tridimensional , Alicerces Teciduais/química , Materiais Biocompatíveis/químicaRESUMO
Bone tissue engineering (BTE) utilizes a special mix of scaffolds, cells, and bioactive factors to regulate the microenvironment of bone regeneration and form a three-dimensional bone simulation structure to regenerate bone tissue. Silk fibroin (SF) is perhaps the most encouraging material for BTE given its tunable mechanical properties, controllable biodegradability, and excellent biocompatibility. Numerous studies have confirmed the significance of SF for stimulating bone formation. In this review, we start by introducing the structure and characteristics of SF. After that, the immunological mechanism of SF for osteogenesis is summarized, and various forms of SF biomaterials and the latest development prospects of SF in BTE are emphatically introduced. Biomaterials based on SF have great potential in bone tissue engineering, and this review will serve as a resource for future design and research.
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Materiais Biocompatíveis , Fibroínas , Materiais Biocompatíveis/química , Engenharia Tecidual/métodos , Fibroínas/química , Alicerces Teciduais/química , Osso e Ossos , Osteogênese , Seda/químicaRESUMO
The process of repairing significant bone defects requires the recruitment of a considerable number of cells for osteogenesis-related activities, which implies the consumption of a substantial amount of oxygen and nutrients. Therefore, the limited supply of nutrients and oxygen at the defect site is a vital constraint that affects the regenerative effect, which is closely related to the degree of a well-established vascular network. Hypoxia-inducible factor (HIF-1α), which is an essential transcription factor activated in hypoxic environments, plays a vital role in vascular network construction. HIF-1α, which plays a central role in regulating cartilage and bone formation, induces vascular invasion and differentiation of osteoprogenitor cells to promote and maintain extracellular matrix production by mediating the adaptive response of cells to changes in oxygen levels. However, the application of HIF-1α in bone tissue engineering is still controversial. As such, clarifying the function of HIF-1α in regulating the bone regeneration process is one of the urgent issues that need to be addressed. This review provides insight into the mechanisms of HIF-1α action in bone regeneration and related recent advances. It also describes current strategies for applying hypoxia induction and hypoxia mimicry in bone tissue engineering, providing theoretical support for the use of HIF-1α in establishing a novel and feasible bone repair strategy in clinical settings.
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Regeneração Óssea , Osso e Ossos , Subunidade alfa do Fator 1 Induzível por Hipóxia , Engenharia Tecidual , Humanos , Regeneração Óssea/genética , Regeneração Óssea/fisiologia , Hipóxia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , OxigênioRESUMO
BACKGROUND: The present study aimed at evaluating the in vitro adsorption capability of chitooligosaccharide (COS) with some metal elements (Fe, Zn, Cd, Pb) at different pH values along with potential effects of dietary COS supplementation on growth performance, mineral content, meat quality and oxidant status in broilers. Day-old male chicks were randomly distributed into two groups and offered a basal diet supplemented with or without 30 mg kg-1 COS for 42 days. RESULTS: In vitro trials demonstrated that Fe levels were higher (P < 0.001) in the COS-treated group compared with the non-treated group at pH of 2.5. However, these levels became lowered when pH values were raised to 5 (P < 0.01) or 6 (P < 0.001). Similarly, COS adsorbed more (P < 0.05) Zn at pH values of 2.5 and 6, and Cd contents at pH of 2.5 for 70 min when compared with the control. For in vivo trial, the feed-to-gain ratio, serum Cu (P < 0.01), hepatic Mn, Cr (P < 0.05) and intramuscular Cd (P < 0.01) were lower in response to COS treatment. Supplementation of COS improved (P < 0.05) meat quality of broilers in terms of lower drip loss, cooking loss and malondialdehyde content with a concomitant increase (P < 0.01) in the pH of breast meat at 24 h post mortem. CONCLUSION: COS adsorbed heavy metal ions not only in vitro but also in broilers, and dietary supplementation with 30 mg kg-1 COS improved growth performance, breast meat quality and oxidant status in broilers. © 2022 Society of Chemical Industry.
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Ração Animal , Galinhas , Animais , Masculino , Ração Animal/análise , Oxidantes , Cádmio , Carne/análise , Minerais , Suplementos Nutricionais/análise , Dieta , AntioxidantesRESUMO
BACKGROUND: The existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation. METHODS: Schneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolated from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium was evaluated by alkaline phosphatase staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery. RESULTS: The cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF could stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which was achieved by up-regulating ERK 1/2 signaling pathway. PRF could accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. CONCLUSIONS: MSCs existed in Schneiderian membrane, and PRF stimulation could promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone. PRF, as autologous graft materials, might offer a promising strategy for the clinical bone formation during MSFE procedure. Video Abstract.
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Células-Tronco Mesenquimais , Fibrina Rica em Plaquetas , Levantamento do Assoalho do Seio Maxilar , Animais , Diferenciação Celular , Células Cultivadas , Seio Maxilar , Células-Tronco Mesenquimais/metabolismo , Mucosa Nasal , Osteogênese , Coelhos , Levantamento do Assoalho do Seio Maxilar/métodosRESUMO
BACKGROUND: The correlations of epidemiological characteristics and clinical outcomes with different tumor sites in patients with intracranial typical site germinomas (ICTSGs) have not yet been well established. We analyzed ICTSGs using a multicenter database, focusing on its demographic, management patterns, and long-term survival outcomes. METHODS: Patients diagnosed with ICTSGs were selected from the Surveillance, Epidemiology, and End-Results (SEER) database. Demographic information and management patterns of ICTSGs were extracted for data analysis stratified by different tumor sites. Kaplan-Meier curves were used to evaluate the survival outcome stratified by treatment, tumor site and tumor size. RESULTS: Among the 327 patients enrolled in the study, 16.21% had tumors located in the suprasellar region and 83.79% in the pineal region. The proportion of males was significantly higher among pineal germinomas (94.16 vs 66.04%; P < .001). Smaller tumors (<24 mm) were more common in the suprasellar region (37.74 vs 18.87%; P < .001). A higher percentage of patients with suprasellar germinomas underwent surgery. Radiotherapy (RT) and chemotherapy (CT) was, respectively, administered to 82.97 and 60.61% of patients during the treatment period, with no significant difference between suprasellar and pineal germinomas. CT plus RT was the most common treatment modality for both pituitary (30.19%) and pineal (33.94%) germinomas. Both RT and CT were associated with improved long-term survival. No survival difference was observed between suprasellar and pineal germinomas. CONCLUSIONS: Despite significant differences in epidemiology and management, pineal and suprasellar germinomas had a similar long-term clinical outcome.
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Neoplasias Encefálicas , Germinoma , Glândula Pineal , Neoplasias da Base do Crânio , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/epidemiologia , Neoplasias Encefálicas/terapia , Germinoma/diagnóstico , Germinoma/patologia , Germinoma/terapia , Humanos , Masculino , Glândula Pineal/patologiaRESUMO
OBJECTIVES: To evaluate patient-reported outcomes and radiographic results of simultaneous implant placement in severely atrophic maxilla using flapless endoscope-assisted osteotome sinus floor elevation with platelet-rich fibrin (PRF), also defined as PESS, and to compare the results with those of lateral sinus floor elevation (LSFE). METHODS: Patients with a residual bone height (RBH) of 2-6 mm were included in a randomised controlled trial. PESS was performed with PRF as the sole grafting material. LSFE was performed using deproteinised bovine bone matrix. Patient-reported outcomes were recorded on a visual analogue scale (VAS-pain) and visual rating scale (VRS-swelling and VRS-willingness). Peri-implant bone height (PBH), bone mineral density (BMD) and sinus grafting remodelling index were measured using CBCT immediately postoperatively and 3rd, 6th and 18th months post-surgery. RESULTS: The study population consisted of 20 patients in each group. The RBH of two groups averaged 3.35 ± 0.79 mm and 2.92 ± 0.63 mm with no significant difference (p > .05). VAS-pain was 18.0 (IR 15.0-22.5) and 35.0 (IR 32.5-37.0) in the PESS and LSFE groups, respectively (p < .01). VAS-pain decreased with time in both groups. VRS-swelling was lower in the PESS group than LSFE group. VRS-willingness was higher in the PESS group than LSFE group (p < .01). At 18 months post-surgery, the marginal bone loss was 0.60 ± 0.25 mm and 0.69 ± 0.35 mm in the two groups with no significant difference (p = .52). CONCLUSIONS: Within the limitations of this study, PESS was associated with lower postoperative morbidity and was more tolerable than LSFE. PESS could be a reliable procedure for sinus floor elevation in patients with insufficient RBH.
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Implantes Dentários , Fibrina Rica em Plaquetas , Levantamento do Assoalho do Seio Maxilar , Animais , Bovinos , Implantação Dentária Endóssea/métodos , Humanos , Maxila/cirurgia , Seio Maxilar/cirurgia , Minerais/uso terapêutico , Dor , Levantamento do Assoalho do Seio Maxilar/métodos , Resultado do TratamentoRESUMO
In recent years, bone tissue engineering (BTE), as a multidisciplinary field, has shown considerable promise in replacing traditional treatment modalities (i.e., autografts, allografts, and xenografts). Since bone is such a complex and dynamic structure, the construction of bone tissue composite materials has become an attractive strategy to guide bone growth and regeneration. Chitosan and its derivatives have been promising vehicles for BTE owing to their unique physical and chemical properties. With intrinsic physicochemical characteristics and closeness to the extracellular matrix of bones, chitosan-based composite scaffolds have been proved to be a promising candidate for providing successful bone regeneration and defect repair capacity. Advances in chitosan-based scaffolds for BTE have produced efficient and efficacious bio-properties via material structural design and different modifications. Efforts have been put into the modification of chitosan to overcome its limitations, including insolubility in water, faster depolymerization in the body, and blood incompatibility. Herein, we discuss the various modification methods of chitosan that expand its fields of application, which would pave the way for future applied research in biomedical innovation and regenerative medicine.
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Quitosana , Engenharia Tecidual , Materiais Biocompatíveis/química , Regeneração Óssea , Osso e Ossos , Quitosana/química , Humanos , Alicerces Teciduais/químicaRESUMO
Sufficient oxygen is required to maintain normal cellular and physiological function, such as a creature's development, breeding, and homeostasis. Lately, some researchers have reported that both pathological hypoxia and environmental hypoxia might affect bone health. Adaptation to hypoxia is a pivotal cellular event in normal cell development and differentiation and in pathological settings such as ischemia. As central mediators of homeostasis, hypoxia-inducible transcription factors (HIFs) can allow cells to survive in a low-oxygen environment and are essential for the regulation of osteogenesis and skeletal repair. From this perspective, we summarized the role of HIF-1 and HIF-2 in signaling pathways implicated in bone development and skeletal repair and outlined the molecular mechanism of regulation of downstream growth factors and protein molecules such as VEGF, EPO, and so on. All of these present an opportunity for developing therapies for bone regeneration.
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Osteogênese , Fator A de Crescimento do Endotélio Vascular , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Humanos , Hipóxia , Subunidade alfa do Fator 1 Induzível por Hipóxia , Osteogênese/fisiologia , Oxigênio/metabolismo , Fatores de Transcrição/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
Immediate implant placement is considered a well-accepted procedure due to its advantages and predictable survival rates. However, data on immediate implantation in posterior sockets with infection and bone defects are limited. The purpose of this clinical report was to describe 3 cases of hopeless posterior teeth with periapical infection and relatively large destruction that were successfully treated with immediate implant placement. The mean follow-up time was 22 months. On the basis of correct clinical decision and treatment procedure, immediate implant placement may be a reliable treatment option for compromised posterior sockets.
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Implantes Dentários , Carga Imediata em Implante Dentário , Humanos , Implantação Dentária Endóssea/métodos , Resultado do Tratamento , Alvéolo Dental/cirurgia , Desbridamento , Extração DentáriaRESUMO
Phototrophic biofilms are easy to grow at sediment/soil-water interfaces (SWIs) in shallow aquatic ecosystems and greatly impact nutrient biogeochemical cycles. However, the pathways by which they contribute to nitrogen interception and interact with sediment/soil remains largely unknown. Here, we conducted a field investigation in paddy fields in various regions of China and found that nitrogen immobilized in biofilm biomass significantly positively correlated with soil organic carbon (SOC) content. A microcosm experiment showed that this was due to increased bacterial and algal diversity, biomass accumulation, and inorganic nitrogen assimilation at high SOC, especially high dissolved organic carbon (DOC) levels. The metatranscriptomics results further verified that many KO groups of PSII, PSI, AP, and PC in antenna proteins and glutamate synthesis were distinctly expressed at elevated SOC and DOC levels. Our results elucidated the effects and possible pathways of how SOC enrichment triggers photosynthesis and nitrogen immobilization by phototrophic biofilms. The results will provide meaningful information for in situ nitrogen interception by using phototrophic biofilms at the SWI in human-made wetlands to change internal nitrogen cycling.
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Carbono , Solo , Biofilmes , Carbono/análise , China , Ecossistema , Humanos , Nitrogênio/análise , ÁguaRESUMO
Rare-earth labeling in biological apatite could provide critical information for the pathologic transition (osteoclastic) and physiologic regeneration (osteogenesis) of bone and teeth because of their characteristic site-sensitive fluorescence in different coordinative conditions of various tissues in many biological processes. However, the rare-earth labeling method for biological apatites, i.e., carbonated-hydroxyapatite, has been rarely found in the literature. In this paper, we report a Pourbaix-diagram guided mineralizing strategy to controllable carbonation and doping of rare-earth ions in the hydroxyapatite (HA) lattice. The carbonation process of hydroxyapatite was achieved by controllable mineralization in hydrothermal condition with K2CO3 as the carbonate source, which results into the pure B-type carbonated hydroxyapatite (CHA) with tunable carbonate substitution degree. All of the as-synthesized materials crystalized into P63/m (No. 176) space group with the lattice parameter of a decreases and c increases with the increasing of carbonate content in the reactants. Structural refinement results revealed that the substitution of planar CO32- is superimposed on one of the faces of PO43- tetrahedral sub-units with a rotation angle of 30° in reference to c-axis. All of the hydrothermally synthesized CHA nanocrystals show hexagonal rod-like morphology with the length of 70-110 nm and diameter of 21-35 nm, and the decreasing length/diameter ratio from 3.61 to 2.96 from low to high carbonated level of the samples. Five rare-earth cations, of Pr3+, Sm3+, Eu3+, Tb3+, and Ho3+, were used as possible probe ions that can be doped into either HA or CHA lattice. The site-preference of Tb3+ doping is the same in the crystallographic site of HA and CHA according to characteristic emission peaks of 5D4-7Fj (j = 3-6) transitions in their photoluminescent spectroscopy. Our work provides a controllable carbonation method for rare-earth labeling hydroxyapatite nanomaterials with potential biologically active implant powders for bone repair and tissue regeneration.
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Substitutos Ósseos/química , Durapatita/química , Metais Terras Raras/química , Nanopartículas/química , Animais , Substitutos Ósseos/farmacologia , Linhagem Celular , Durapatita/farmacologia , Medições Luminescentes , Metais Terras Raras/farmacologia , CamundongosRESUMO
In order to investigate the effects of dietary ginger extract (GE) enriched in gingerols on broilers under heat stress (HS) from 21 to 42 days of age, a total of 144 Ross 308 male broilers were randomly allocated to three groups with six replicates of eight broilers per replicate. Broilers in the control group were raised at 22 °C and fed a basal diet, and broilers in the other two groups were raised under cyclic HS (34 °C from 9:00 to 17:00 and at 22 °C for the rest of the time) and fed the basal diet with or without 1000 mg/kg GE. Supplementation of GE improved (P < 0.05) final body weight, average daily gain and feed conversion ratio of broilers under HS, and tended (P < 0.1) to increase breast muscle yield. The alterations of serum total protein, albumin, total cholesterol levels and aspartate aminotransferase activity under HS were reversed (P < 0.05) by GE, which also decreased (P < 0.05) serum triglyceride level and alanine aminotransferase activity. The decreased redness (a* value) and increased drip loss of breast muscle induced by HS were restored (P < 0.05) by GE. Moreover, GE supplementation increased (P < 0.05) total antioxidant capacity and decreased (P < 0.05) malondialdehyde content in liver and breast muscle, and increased (P < 0.05) glutathione peroxidase activity in serum and breast muscle. In conclusion, dietary GE supplementation restored growth performance, serum metabolites and meat quality of broilers under HS possibly by improving antioxidant activity.
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Antioxidantes/farmacologia , Tamanho Corporal/efeitos dos fármacos , Catecóis/farmacologia , Galinhas/fisiologia , Álcoois Graxos/farmacologia , Resposta ao Choque Térmico/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Proteínas Sanguíneas/análise , Galinhas/sangue , Galinhas/crescimento & desenvolvimento , Colesterol/sangue , Zingiber officinale/química , Glutationa Peroxidase/sangue , Glutationa Peroxidase/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Malondialdeído/sangue , Malondialdeído/metabolismo , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Produtos Avícolas/normas , Triglicerídeos/sangueRESUMO
This study was conducted to evaluate the effects of dietary supplementation with yeast extract on growth, body composition, non-specific immunity, and antioxidant status of Chinese mitten crab (Eriocheir sinensis). A total of 432 crabs (initial average weight, 4.62⯱â¯0.11â¯g) were randomly distributed into four treatment groups with six replicates of 18 crabs. The crabs were fed a basal diet or the same diet supplemented with 2.5, 5, and 10â¯g/kg yeast extract for 8 weeks. The results showed that dietary yeast extract inclusion enhanced the edible viscera index (linear, Pâ¯<â¯0.001), edible viscera crude protein (CP) content (linear, Pâ¯=â¯0.025) and serum phenoloxidase (ProPO) activity (quadratic, Pâ¯=â¯0.023) at 56 day, increased the total superoxide dismutase (T-SOD) activity at 28 day (quadratic, Pâ¯=â¯0.037) and catelase (CAT) activity at 56 day (quadratic, Pâ¯=â¯0.034) of edible viscera, and muscular T-SOD activity (quadratic, Pâ¯=â¯0.020) at 56 day in Chinese mitten crab. Compared with the control group, the inclusion of 5â¯g/kg yeast extract in the diet increased the edible viscera index, enhanced the CAT activity of edible viscera at 56 day in Chinese mitten crab (Pâ¯<â¯0.05). Dietary 10â¯g/kg yeast extract inclusion enhanced the edible viscera index at 56 day in Chinese mitten crab than that of the control group (Pâ¯<â¯0.05). These results implied that dietary yeast extract inclusion improved the edible viscera index and crude protein content of edible viscera, enhanced serum immunity, and increased the antioxidant status of edible viscera and muscle in Chinese mitten crab, especially when it is supplemented at 5â¯g/kg yeast extract in the diet.
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Ração Animal/análise , Braquiúros/fisiologia , Suplementos Nutricionais , Saccharomyces cerevisiae , Animais , Antioxidantes/análise , Composição Corporal , Braquiúros/imunologia , Dieta/veterinária , Imunidade InataRESUMO
PURPOSE: The purpose of this study was to describe a new case using implant-retained overdentures in a patient with severe oral lichen planus (OLP) and to perform a literature review to evaluate the effectiveness and safety of implant rehabilitation in OLP patients. MATERIAL AND METHODS: In our patient with erosive OLP, restoration was performed with implant-retained overdentures using the Locator attachment system (Zest Anchors, Escondido, CA). Our case showed favorable restorative results and excellent implant osseointegration with acceptable marginal bone resorption during the follow-up period of 3 years. We performed a literature search of the PubMed (Medline), Embase, and Cochrane databases for articles published between 1990 and 2018 using the key terms "oral lichen planus" and "implant." RESULTS: This search identified 13 publications, including 9 single case reports or case series and 4 small-scale controlled studies. A total of 86 OLP patients and 259 dental implants were analyzed. The survival rate of implants was 95.8% during a follow-up period ranging from 1 to 13 years. CONCLUSION: Dental implants seem to be an acceptable and reliable treatment option in patients with OLP. Nevertheless, clinical information on this topic is still scarce, and more well-designed randomized studies are needed to define the benefits and risks of implant rehabilitation in OLP patients.