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1.
Inj Prev ; 27(6): 521-526, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-33443032

RESUMO

OBJECTIVE: This study was designed to reveal the relationship between the use and type of eye protection and the occurrence of work-related corneal and conjunctival foreign body injuries. METHODS: This is a retrospective cohort study of patients with work-related corneal and/or conjunctival foreign body injuries between 1 August 2017 and 31 July 2018. They were all diagnosed and treated at Jia Ding Hospital affiliated to the Shanghai University of Medicine and Health Sciences in Shanghai, China. All patients received a comprehensive eye examination and a face-to-face interview using a structured questionnaire by ophthalmologists. RESULTS: A total of 426 consecutive patients were included in the study. The majority of work-related eye injuries occurred in men (94.17%). Summer was the season that had the highest incidence of eye injuries, especially in July and August (38.03%). There were 290 patients (68.08%) that were injured more than once. The ratio of eye protection use to non-protection was 1:7 at the first time of eye injury. The ratio improved to 1:3 on subsequent injury. A majority of employers (79.11%) provided eye protection to employees. However, 19.95% of the workers were injured despite wearing a pair of protective spectacles. The causes of work-related eye injury were as follows: no eye protections provided (20.89%); unawareness of work safety (30.99%); defect of spectacles (47.18%). CONCLUSIONS: Protection use at work effectively prevents work-related eye injuries. Both employers and employees require improved awareness of workplace hazards and personal protection. Eye protection should be selected appropriately according to the work environment.


Assuntos
Traumatismos Oculares , Corpos Estranhos , China/epidemiologia , Traumatismos Oculares/epidemiologia , Traumatismos Oculares/prevenção & controle , Dispositivos de Proteção dos Olhos , Humanos , Masculino , Estudos Retrospectivos
2.
Molecules ; 26(22)2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34833887

RESUMO

Pyropia haitanensis is an important laver species in China. Its quality traits are closely related to the content of glutamic acid. Glutamate dehydrogenase (GDH) is a crucial enzyme in the glutamic acid metabolism. In this study, two GDH genes from P. haitanensis, PhGDH1 and PhGDH2, were cloned and successfully expressed in Escherichia coli. The in vitro enzyme activity assay demonstrated that the catalytic activity of PhGDHs is mainly in the direction of ammonium assimilation. The measured Km values of PhGDH1 for NADH, (NH4)2SO4, and α-oxoglutarate were 0.12, 4.99, and 0.16 mM, respectively, while the corresponding Km values of PhGDH2 were 0.02, 3.98, and 0.104 mM, respectively. Site-directed mutagenesis results showed that Gly193 and Thr361 were important catalytic residues for PhGDH2. Moreover, expression levels of both PhGDHs were significantly increased under abiotic stresses. These results suggest that PhGDHs can convert α-oxoglutarate to glutamic acid, and enhance the flavor and stress resistance of P. haitanensis.


Assuntos
Glutamato Desidrogenase/metabolismo , Ácido Glutâmico/metabolismo , Rodófitas/metabolismo , Fenômenos Bioquímicos , China , Glutamato Desidrogenase/fisiologia , Mutagênese Sítio-Dirigida , Rodófitas/genética , Estresse Fisiológico/fisiologia
3.
PLoS Pathog ; 13(9): e1006625, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28938017

RESUMO

Enterovirus 71 (EV71) is the major causative agent of hand, foot and mouth disease (HFMD) in children, causing severe clinical outcomes and even death. Here, we report an important role of the highly conserved alanine residue at position 107 in the capsid protein VP1 (VP1A107) in the efficient replication of EV71. Substitutional mutations of VP1A107 significantly diminish viral growth kinetics without significant effect on viral entry, expression of viral genes and viral production. The results of mechanistic studies reveal that VP1A107 regulates the efficient cleavage of the VP0 precursor during EV71 assembly, which is required, in the next round of infection, for the transformation of the mature virion (160S) into an intermediate or A-particle (135S), a key step of virus uncoating. Furthermore, the results of molecular dynamic simulations and hydrogen-bond networks analysis of VP1A107 suggest that flexibility of the VP1 BC loop or the region surrounding the VP1107 residue directly correlates with viral infectivity. It is possible that sufficient flexibility of the region surrounding the VP1107 residue favors VP0 conformational change that is required for the efficient cleavage of VP0 as well as subsequent viral uncoating and viral replication. Taken together, our data reveal the structural role of the highly conserved VP1A107 in regulating EV71 maturation. Characterization of this novel determinant of EV71 virulence would promote the study on pathogenesis of Enteroviruses.


Assuntos
Enterovirus Humano A/fisiologia , Infecções por Enterovirus/virologia , Células Vero/virologia , Replicação Viral/genética , Aminoácidos/genética , Animais , Proteínas do Capsídeo/metabolismo , Chlorocebus aethiops , Mutação de Sentido Incorreto/genética , Internalização do Vírus
4.
J Neurosci ; 33(7): 3178-89, 2013 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-23407971

RESUMO

Although rhodopsin is essential for sensing light for vision, it also mediates light-induced apoptosis of photoreceptors in mouse. RPE65, which catalyzes isomerization of all-trans retinyl fatty acid esters to 11-cis-retinol (11cROL) in the visual cycle, controls the rhodopsin regeneration rate and photoreceptor susceptibility to light-induced degeneration. Mutations in RPE65 have been linked to blindness in affected children. Despite such importance, the mechanism that regulates RPE65 function remains unclear. Through unbiased expression screening of a bovine retinal pigment epithelium (RPE) cDNA library, we have identified elongation of very long-chain fatty acids-like 1 (ELOVL1) and fatty acid transport protein 4 (FATP4), which each have very long-chain fatty acid acyl-CoA synthetase (VLCFA-ACS) activity, as negative regulators of RPE65. We found that the VLCFA derivative lignoceroyl (C24:0)-CoA inhibited synthesis of 11cROL, whereas palmitoyl (C16:0)-CoA promoted synthesis of 11cROL. We further found that competition of FATP4 with RPE65 for the substrate of RPE65 was also involved in the mechanisms by which FATP4 inhibits synthesis of 11cROL. FATP4 was predominantly expressed in RPE, and the FATP4-deficient RPE showed significantly higher isomerase activity. Consistent with these results, the regeneration rate of 11-cis-retinaldehyde and the recovery rate for rod light sensitivity were faster in FATP4-deficient mice than wild-type mice. Moreover, FATP4-deficient mice displayed increased accumulation of the cytotoxic all-trans retinaldehyde and hypersusceptibility to light-induced photoreceptor degeneration. Our findings demonstrate that ELOVL1, FATP4, and their products comprise the regulatory elements of RPE65 and play important roles in protecting photoreceptors from degeneration induced by light damage.


Assuntos
Proteínas de Transporte de Ácido Graxo/farmacologia , Luz , Células Fotorreceptoras Retinianas Cones/efeitos dos fármacos , Degeneração Retiniana/prevenção & controle , Células Fotorreceptoras Retinianas Bastonetes/efeitos dos fármacos , cis-trans-Isomerases/antagonistas & inibidores , Acetiltransferases/farmacologia , Oxirredutases do Álcool/metabolismo , Animais , Western Blotting , Células Cultivadas , Eletrorretinografia , Elongases de Ácidos Graxos , Proteínas de Transporte de Ácido Graxo/genética , Regulação da Expressão Gênica/fisiologia , Biblioteca Gênica , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Elongação Traducional da Cadeia Peptídica , Fenótipo , Reação em Cadeia da Polimerase em Tempo Real , Células Fotorreceptoras Retinianas Cones/efeitos da radiação , Células Fotorreceptoras Retinianas Bastonetes/efeitos da radiação , Retinoides/metabolismo , Transfecção , cis-trans-Isomerases/genética , cis-trans-Isomerases/metabolismo
5.
Mol Vis ; 19: 1747-59, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23922492

RESUMO

PURPOSE: Neuroprotectin D1 (NPD1) attenuates laser-induced choroidal neovascularization (CNV) when administered intraperitoneally. Due to its lipophilicity and low molecular weight, NPD1 is well suited for topical delivery; thus, we investigated the efficacy of topically applied NPD1 in attenuating CNV. We also examined the effect of NPD1 on the recruitment and activation of microglia surrounding CNV lesions. METHODS: Mice were given laser-induced CNV and treated with NPD1 eye drops. CNV was evaluated by fluorescein leakage using a novel image analysis method and by isolectin B4 immunofluorescence of neovasculature. Microglia; recruitment was assessed by quantification. Using form factor, solidity, convexity, and fractal dimension, microglial activation was quantitatively assessed by two-dimensional, and for the first time, three-dimensional morphology. An ImageJ plugin, 3D Shape, was developed to enable this analysis. RESULTS: NPD1 attenuated leakage and neovascularization. The proximity of microglia to CNV lesions was significantly closer with NPD1. Consistent with the cellular ramification, microglia in NPD1-treated eyes were larger and exhibited a lower form factor and higher fractal dimension. CONCLUSIONS: Our data show that NPD1 signaling induces a ramified, non-injury-inducing microglial phenotype coincident with attenuation of CNV. Since microglia are crucial participants in neurodegenerative diseases, the discovery that microglia are potential targets of NPD1 signaling warrants further investigation.


Assuntos
Neovascularização de Coroide/tratamento farmacológico , Ácidos Docosa-Hexaenoicos/uso terapêutico , Microglia/patologia , Administração Tópica , Animais , Forma Celular/efeitos dos fármacos , Neovascularização de Coroide/diagnóstico por imagem , Neovascularização de Coroide/patologia , Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácidos Docosa-Hexaenoicos/farmacologia , Angiofluoresceinografia , Lasers , Masculino , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Microglia/efeitos dos fármacos , Radiografia
6.
Exp Eye Res ; 108: 59-67, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23274584

RESUMO

Our previous study on retinal light exposure suggests the involvement of zinc (Zn(2+)) toxicity in the death of RPE and photoreceptors (LD) which could be attenuated by pyruvate and nicotinamide, perhaps through restoration of NAD(+) levels. In the present study, we examined Zn(2+) toxicity, and the effects of NAD(+) restoration in primary retinal cultures. We then reduced Zn(2+) levels in rodents by reducing Zn(2+) levels in the diet, or by genetics and measured LD. Sprague Dawley albino rats were fed 2, or 61 mg Zn(2+)/kg of diet for 3 weeks, and exposed to 18 kLux of white light for 4 h. We light exposed (70 kLux of white light for 50 h) Zn(2+) transporter 3 knockout (ZnT3-KO, no synaptic Zn(2+)), or RPE65 knockout mice (RPE65-KO, lack rhodopsin cycling), or C57/BI6/J controls and determined light damage and Zn(2+) staining. Retinal Zn(2+) staining was examined at 1 h and 4 h after light exposure. Retinas were examined after 7 d by optical coherence tomography and histology. After LD, rats fed the reduced Zn(2+) diet showed less photoreceptor Zn(2+) staining and degeneration compared to a normal Zn(2+) diet. Similarly, ZnT3-KO and RPE65-KO mice showed less Zn(2+) staining, NAD(+) loss, and RPE or photoreceptor death than C57/BI6/J control mice. Dietary or ZnT3-dependent Zn(2+) stores, and intracellular Zn(2+) release from rhodopsin recycling are suggested to be involved in light-induced retinal degeneration. These results implicate novel rhodopsin-mediated mechanisms and therapeutic targets for LD. Our companion manuscript demonstrates that pharmacologic, circadian, or genetic manipulations which maintain NAD(+) levels reduce LD.


Assuntos
Dieta , Luz/efeitos adversos , Proteínas de Membrana/deficiência , Células Fotorreceptoras de Vertebrados/efeitos dos fármacos , Células Fotorreceptoras de Vertebrados/efeitos da radiação , Degeneração Retiniana/prevenção & controle , Zinco/toxicidade , Animais , Proteínas de Transporte/genética , Proteínas de Transporte de Cátions , Morte Celular/efeitos dos fármacos , Morte Celular/efeitos da radiação , Células Cultivadas , Modelos Animais de Doenças , Proteínas de Membrana/genética , Proteínas de Membrana Transportadoras , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , NAD/metabolismo , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/patologia , Ratos , Ratos Sprague-Dawley , Degeneração Retiniana/etiologia , Degeneração Retiniana/genética , Degeneração Retiniana/metabolismo , Degeneração Retiniana/patologia , Rodopsina/metabolismo , Fatores de Tempo , Tomografia de Coerência Óptica , Zinco/administração & dosagem , Zinco/metabolismo , cis-trans-Isomerases/deficiência , cis-trans-Isomerases/genética
7.
Mitochondrial DNA B Resour ; 8(3): 414-417, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36969326

RESUMO

Leuciscus merzbacheri (Zugmayer, 1912) is a cyprinid fish endemic to China, with a distribution range limited to Xinjiang Province. As a landmark species in the Junggar Basin, L. merzbacheri is of considerable significance regarding our understanding of the adaptive evolution of salt and alkali tolerance. In this study, the complete mitochondrial sequence of L. merzbacheri was obtained for the first time by high-throughput sequencing. The circular mitogenome is 16,609 bp in length and contains the standard 37 genes, including 13 protein-coding, 22 transfer RNA, and 2 ribosomal RNA genes, which is similar to that of other fish. The mitogenome contents of A, T, C, and G were 27.9, 26.3, 27.1, and 18.7%, respectively. Phylogenetically, L. merzbacheri was located on a new branch near the base of the phylogenetic tree, thereby suggesting an early origin.

8.
Ying Yong Sheng Tai Xue Bao ; 34(2): 535-546, 2023 Feb.
Artigo em Zh | MEDLINE | ID: mdl-36803732

RESUMO

The fertilized eggs of cuttlefish are sticky eggs. Cuttlefish parents prefer to lay eggs on the attached substrates, which help increase the number of eggs and the hatching rate of fertilized eggs. If egg-attached substrates are sufficient, cuttlefish spawning will be reduced or even delayed. With the advances in the construction of marine nature reserves and research on artificial enrichment techniques, domestic and international experts have conducted research on different types and configurations of attachment substrates around cuttlefish resource enhancement. Based on the source of the substrates, we classified cuttlefish spawning substrates into two types, natural and artificial. By summarizing the differences, advantages, and disadvantages of the common economic cuttlefish spawning substrates in offshore areas worldwide, we sort out the functions of two different types of attachment bases, and discussed the practical applications of natural and artificial egg-attached substrates in spawning ground restoration and artificial enrichment. We proposed several thoughts on the future research directions of cuttlefish spawning attachment substrates, aiming to provide reasonable suggestions for cuttlefish habitat restoration, cuttlefish breeding and sustainable development of fishery resources.


Assuntos
Decapodiformes , Ecossistema , Animais , Comportamento Animal , Pesqueiros
9.
Genes (Basel) ; 14(7)2023 07 24.
Artigo em Inglês | MEDLINE | ID: mdl-37510412

RESUMO

Larimichthys crocea (also known as the large yellow croaker) is one of the most economically important marine fishes in China, and research on the ecology and genetics of this species is of immense significance. In this study, we performed restriction site-associated DNA sequencing (RAD-seq) of 54 individuals collected from four sites in China to analyze the genetic structure and diversity of large yellow croaker at the genome level. It revealed that the large yellow croaker populations in the Ningde and Zhoushan coastal waters can be clearly distinguished. Different genetic diversity indices were used to analyze the genetic diversity of the large yellow croaker, which showed that there was a differentiation trend between the wild and farmed populations in Ningde. Moreover, we identified genetically differentiated genomic regions between the populations. GO gene enrichment analysis identified genes that are related to fatty acid metabolism and growth. These findings enhance our understanding of genetic differentiation and adaptation to different living environments, providing a theoretical basis for the preservation and restoration of the genetic resources of the large yellow croaker.


Assuntos
Genoma , Perciformes , Animais , Genoma/genética , Perciformes/genética , Peixes/genética , Genômica , Sequência de Bases
10.
Ying Yong Sheng Tai Xue Bao ; 34(1): 242-248, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36799400

RESUMO

Trichiurus japonicus is an economically valuable species in the East China Sea, whose allowable capture size varies with fishing effort in different years. To clarify the optimum capture size for T. japonicus in the East China Sea, we collected samples and data from T. japonicus targeting fishing gears such as trawls, canvas spreader stow nets and longlines from 2016 to 2020. We estimated growth and mortality parameters using the FiSAT II software, and calculated the size limit standards for capture. The results showed that the inflection point of anal length, the critical anal length and the anal length of one-year-old T. japonicus was 382.84, 397.12, and 216.05 mm, respectively. The anal length of maturity was 230.38 mm, and the minimum capture size (anal length) was 219.23 mm. Based on the yield per recruitment analysis using Beverton-Holt model, the current fishery reference points were under overfishing condition (tc=0.38 a, F=2.11), and the suggested optimum capture size was 364.64 mm. There would be a sharp decline of T. japonicus catches in the East China Sea if the suggested minimum capture size was substantially higher, which might be non-practical for local fishermen. Therefore, we suggested 220 mm as the capture size limit, which was close to the anal length of one-year-old fish and the anal length of maturity.


Assuntos
Conservação dos Recursos Naturais , Perciformes , Animais , Pesqueiros , China
11.
ACS Omega ; 7(21): 17921-17928, 2022 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-35664597

RESUMO

To improve the hydrophobicity of precious hardwood, a facile sanding pretreatment and alkyl ketene dimer (AKD) modification were performed. After the AKD modification, the wood was highly hydrophobic, and the contact angle was 143°. The increased hydrophobicity could be attributed to the ester bond formed between the wood hydroxyl groups and AKD, which was confirmed by attenuated total reflectance-Fourier transform infrared spectroscopy. Sanding pretreatment could further greatly increase the wood hydrophobicity and render it superhydrophobic, not only in a cross-section but also in the tangential and radial sections. The changed wood surface roughness could be responsible for the increased hydrophobicity, which was confirmed by characterization with a scanning electron microscope (SEM) and a three-dimensional optical microscope. Apart from the improved hydrophobicity, the AKD-modified wood exhibited excellent water, acid, and toluene resistance. After a 12 h immersion, the contact angle did not change significantly, and the acid immersion contributed to an improvement in the hydrophobicity of the wood. Furthermore, the resultant AKD-modified wood exhibited an excellent self-cleaning effect.

12.
Polymers (Basel) ; 14(22)2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36433171

RESUMO

Due to wood moisture sensitivity, shrinkage cracks tend to present wooden structures. These failures are caused by moisture-related shrinkage behavior. In order to avoid it, it is necessary to have a better understanding of shrinkage behavior. In this respect, studying the dimension changes in wood at different scales is of utmost significance for a better understanding of the shrinkage properties. Herein, the shrinkage behavior of Masson pines (Pinus massoniana) wood was investigated at macroscopic and cellular levels during moisture loss via digital image correlation using VIC-3D and digital microscopic systems, respectively. According to the full-field strain maps, shrinkage strain near the external face was higher than that at the internal face, which increased susceptibility to cracking at the external face of lumber. Additionally, the anisotropic shrinkage of wood was explored. The shrinkage ratio at the end of drying was about 5.5% in the tangential (T) direction and 3.5% in the radial (R) direction. However, at a cellular level, the shrinkage ratios in the T and R directions of earlywood tracheids were 7.13% and 2.46%, whereas the corresponding values for latewood tracheid were 9.27% and 5.52%, respectively. Furthermore, the maximum T/R shrinkage ratio at the macroscopic level (1.7) was found to be similar to the value of latewood tracheid (1.72). The earlywood showed high anisotropic, its T/R shrinkage ratio was 2.75. The macroscopic shrinkage was the result of the interaction of the tracheids of earlywood and latewood and was mainly dominated by latewood tracheids.

13.
Mar Pollut Bull ; 185(Pt A): 114226, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36272319

RESUMO

The spatial-seasonal distributions and variations, correlations with environmental variables and the pollution degrees of dissolved metals in the Zhejiang coastal seawater were investigated. The concentrations of six dissolved metals (i.e. Cu, Pb, Zn, Cd, As and Hg) were in the ranges of 0.10-6.40 (1.6 ± 0.8), 0.16-3.60 (1.2 ± 0.7), 2.50-24.0 (8.5 ± 4.8), 0.011-0.180 (0.07 ± 0.03), 0.85-4.20 (2.1 ± 0.8) and 0.001-0.110 (0.06 ± 0.02) µg/L, throughout the four seasons, respectively. Significant differences in all the dissolved metals were found among seasons, whereas no significant differences were found among stations. The average concentrations of metals were in the following order: Zn > As>Cu > Pb > Cd > Hg. Single metal contamination factor was in the following order: Pb > Hg > Zn > Cu > As>Cd. The pollution level of dissolved metals in the Zhejiang coastal waters (ZCW) was low. Most of the dissolved metals were correlated to temperature, indicating seasonal differences. The redundancy analysis (RDA) indicated that depth, temperature, nitrate and phosphate could best explain the variance pattern of dissolved metals in the ZCW.


Assuntos
Mercúrio , Metais Pesados , Poluentes Químicos da Água , Estações do Ano , Metais Pesados/análise , Monitoramento Ambiental , Poluentes Químicos da Água/análise , Cádmio/análise , Chumbo/análise , Água do Mar/análise , Mercúrio/análise , China
14.
Sci Total Environ ; 807(Pt 1): 150643, 2022 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-34597545

RESUMO

As well-known emergent environmental contaminants, polyhalogenated carbazoles (PHCZs) have recently received increasing attention. In this study, we investigated the concentrations of carbazole (CZ) and PHCZs in 70 marine organisms from the East China Sea (ECS). CZ and 9-11 PHCZs were detected in organisms from the ECS, with concentrations in the range of 0.75-33 ng/g lipid weight, lw and 4.3-113 ng/g lw, respectively. Among the PHCZs, there were 3-4 major components in zooplankton, fish, shrimp, crabs, snails and shellfish, and the sum of these major components accounted for 59% to 67% of ∑PHCZs. The bioaccumulation potentials of 1,3,6,8-tetrachlorocarbazole (1368-CCZ) and 3-chlorocarbazole (3-CCZ) from water were observed. The logarithmic bioaccumulation factor (logBAF) values of the CZ and PHCZs increased significantly with increasing logKOW values (R = 0.449-0.784, p < 0.01). The trophic magnification factor (TMF) values of the CZ, 9 PHCZs and ∑PHCZs were calculated to be 3.32, 1.87-4.06 and 2.36, respectively, indicating the potential biomagnification of the CZ and PHCZs in the zooplankton-shrimp-fish food web. The toxic equivalents (TEQs) of PHCZs in organisms from the ECS were in the range of 0.78-36 pg TEQ/g lw. Overall, for the first time, this study systematically examined the occurrence, bioaccumulation and potential risk of PHCZs in the marine food web of the East China Sea.


Assuntos
Organismos Aquáticos , Poluentes Químicos da Água , Animais , Bioacumulação , Carbazóis , China , Monitoramento Ambiental , Peixes , Cadeia Alimentar , Poluentes Químicos da Água/análise
15.
Ying Yong Sheng Tai Xue Bao ; 33(8): 2244-2250, 2022 Aug.
Artigo em Zh | MEDLINE | ID: mdl-36043833

RESUMO

In order to understand the growth dynamics of Larimichthys polyactis in spring and autumn along the coast of Zhejiang Province, we used the survey data of bottom trawlers in spring (April) and autumn (November) from 2014 to 2019 along the coast of Zhejiang Province to examine the heterogeneity of L. polyactis growth by constructing a generalized linear model (GLM) and nine linear mixed effect models (LMEM). The results showed that body length of L. polyactis ranged from 15 mm to 210 mm, with an average of 124.12 mm. The dominant body length group ranged from 110 mm to 140 mm. The body weight ranged from 0.04 g to 156.2 g, with an average of 33.28 g. The dominant body weight group ranged from 30 to 50 g. According to the AIC minimum criterion, the LMEM model with the random effects of seasons and water zones on growth parameters a and b was the best. The results of cross validation also showed that the prediction of this model was the best. In the optimal model, the fixed value of growth parameter a was 0.61×10-4, which ranged in (0.32-1.91)×10-4 after adding season and water random effect. The fixed value of parameter b was 2.73, and ranged from 2.49 to 2.86 after adding the random effects of season and water area, indicating that small yellow croaker was under negative allometric growth. Season and water zone had significant effects on the relationship between body length and body weight of small yellow croaker. From a seasonal perspective, the growth rate of small yellow croaker in spring was higher than that in autumn. From water zone perspective, the closer the offshore distance, the faster the growth rate of small yellow croaker.


Assuntos
Perciformes , Animais , Peso Corporal , China , Modelos Lineares , Estações do Ano , Água
16.
Exp Eye Res ; 92(2): 155-60, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21035444

RESUMO

Spectral Domain Optical Coherence Tomography (SD-OCT) applied to the mouse retina has been limited due to inherent movement artifacts and lack of resolution. Recently, SD-OCT scans from a commercially available imaging system have yielded retinal thickness values comparable to histology. However, these measurements are based on single point analysis of images. Here we report that using the Spectralis HRA + OCT Spectral Domain OCT and Fluorescein Angiography system (Heidelberg Engineering, Heidelberg, Germany), retinal thickness of linear expanses from SD-OCT data can be accurately assessed. This is possible by the development of a Spectralis-compatible ImageJ plug-in that imports 8-bit SLO and 32-bit OCT B-scan images, retaining scale and segmentation data and enabling analysis and 3D reconstruction. Moreover, mouse retinal layer thickness values obtained with this plug-in exhibit a high correlation to thickness measurements from histology of the same retinas. Thus, use of this ImageJ plug-in results in reliable quantification of long retinal expanses from in vivo SD-OCT images.


Assuntos
Células Fotorreceptoras de Vertebrados/citologia , Epitélio Pigmentado da Retina/citologia , Tomografia de Coerência Óptica , Animais , Antropometria , Biometria/métodos , Angiofluoresceinografia , Masculino , Camundongos , Camundongos Endogâmicos BALB C
17.
Exp Eye Res ; 93(5): 636-48, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21854772

RESUMO

Retinal pathologies common to human eye diseases, including abnormal retinal pigment epithelial (RPE) cells, drusen-like accumulation, photoreceptor atrophy, and choroidal neovascularization, have been reported in the Ccl2/Cx3cr1-deficient mouse. The Ccl2 gene encodes the pro-inflammatory chemokine CCL2 (MCP-1), which is responsible for chemotactic recruitment of monocyte-derived macrophages to sites of inflammation. The Cx3cr1 gene encodes the fractalkine receptor, CX3CR1, and is required for accumulation of monocytes and microglia recruited via CCL2. Chemokine-mediated inflammation is implicated in retinal degenerative diseases such as diabetic retinopathy, age-related macular degeneration, retinitis pigmentosa, and uveoretinitis, and proper chemokine signaling from the RPE, Müller glia, and astrocytes is necessary to regulate leukocyte trafficking. Therefore, this mouse, possessing aberrant chemokine signaling coupled with retinal degenerative pathologies, presents an ideal opportunity to investigate the effect of altered signaling on retinal homeostasis and photoreceptor degeneration. Since this mouse is a recent development, more data covering the onset, location, and progression rate of pathologies is needed. In the present study we establish these parameters and show two photoreceptor cell death processes. Our observations of decreased glutamine synthetase and increased glial fibrillary acidic protein suggest that Müller cells respond very early within regions where lesions are forming. Finally, we suggest that retinal angiomatous proliferation contributes to pathological angiogenesis in this Ccl2/Cx3cr1-deficient mouse.


Assuntos
Quimiocina CCL2/fisiologia , Modelos Animais de Doenças , Células Fotorreceptoras de Vertebrados/patologia , Receptores de Quimiocinas/fisiologia , Degeneração Retiniana/patologia , Tomografia de Coerência Óptica , Animais , Western Blotting , Receptor 1 de Quimiocina CX3C , Progressão da Doença , Angiofluoresceinografia , Proteína Glial Fibrilar Ácida , Gliose/metabolismo , Gliose/patologia , Glutamato-Amônia Ligase/metabolismo , Imageamento Tridimensional , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteínas do Tecido Nervoso/metabolismo , Oftalmoscopia , Degeneração Retiniana/metabolismo
18.
Mar Pollut Bull ; 170: 112670, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34186447

RESUMO

The concentration, constitution, distribution, possible sources, and associated consumption risks of polycyclic aromatic hydrocarbons (PAHs) in four marine fishes in the Zhoushan Archipelago were investigated. The concentrations of PAHs in the edible muscle of these four fishes ranged from 34.7 to 108 ng/g wet weight. Four-ring and six-ring PAH congeners constitute the most and least percentages of the total PAHs, respectively. The highest PAH concentrations were found in Mugil cephalus, followed by Sebastiscus marmoratus, Lateolabrax maculatus, and Collichthys lucidus. The highest PAH concentrations were found at the sampling island Liuheng, followed by Gouqi, Qushan, Dongji, and Zhujiajian. PAH concentrations in wet weight were remarkably different among these four fish species but not among different locations. High-molecular-weight congeners predominated the PAH composition pattern in most of the samples. Results showed that the consumption of M. cephalus might have potential carcinogenic risk. This study provided baseline data on PAH concentrations in seafood and consequent human consumption risk.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , China , Monitoramento Ambiental , Peixes , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Medição de Risco , Poluentes Químicos da Água/análise
19.
Mar Pollut Bull ; 173(Pt B): 113049, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34673427

RESUMO

As new persistent organic pollutants, short chain chlorinated paraffins (SCCPs) have recently received particular attention. However, knowledge on their bioaccumulation and trophic magnification in marine organisms from East China Sea (ECS) is still scare. In this study, we investigated the concentrations of SCCPs in seawater (n = 15) and marine organisms (n = 88) collected from ECS. The total concentrations of SCCPs (∑SCCPs) ranged from 12.5 to 242 ng/L in seawater and from 12.8 to 1819 ng/g wet weight (ww) in organisms. C10-11 SCCPs and Cl5-7 SCCPs were the predominant homologues in all samples, with the mean proportions of 70 ± 6.5% and 80 ± 7.8% in seawater, as well as 52-77% and 61-84% in marine organisms, respectively. The logarithm bioaccumulation factor (log BAF) values of ∑SCCPs were in the range of 2.04-3.79 in zooplankton, fish, shrimp, crab, shellfish, snail, and cephalopod. The log BAF values of SCCP homologues (1.33-4.75) increased significantly with the increase of their logarithm octanol-water partition coefficients (log KOW) values, indicating that hydrophobicity is the major factor controlling the bioaccumulation of SCCPs. The trophic magnification factor (TMF) value of ∑SCCPs was calculated to be 3.98, indicating the potential trophic magnification of SCCPs in this marine food web from ECS. A slightly increasing trend was observed between the TMF values of SCCP homologues and their log KOW values. Overall, for the first time, this study systematically examined the bioaccumulation and trophic magnification of SCCPs in the marine food web from East China Sea.


Assuntos
Hidrocarbonetos Clorados , Parafina , Animais , Organismos Aquáticos , Bioacumulação , China , Monitoramento Ambiental , Cadeia Alimentar , Hidrocarbonetos Clorados/análise , Parafina/análise
20.
Ying Yong Sheng Tai Xue Bao ; 32(4): 1489-1497, 2021 Apr.
Artigo em Zh | MEDLINE | ID: mdl-33899418

RESUMO

To elucidate the characteristics of fishery resources structure in the Dachenyang Spaw-ning Ground Reserve, the index of relative importance (IRI), biodiversity index and canonical correspondence analysis (CCA) were used to explore the relationship between crustaceans community and marine environment based on the bottom trawl survey data collected from April and November in 2018. A total of 38 crustaceans species were recorded, belonged to 25 genera in 14 families. The dominant species were Oratosquilla oratoria, Portunus trituberculatus, Charybdis bimaculata, and Parapenaeopsis hardwickii. The resource density of crustaceans was generally high in depths below 50 m in spring and in depths above 50 m in autumn. The density in the southern area was higher than nouthern area. The Margalef species richness index (D), Shannon diversity index (H) and Pielou evenness index (J) of crustaceans in spring was higher than that in autumn. The H of crustaceans in depths above 50 m was the highest. Based on cluster analysis and non-matrix multidimentional scaling analysis, the crustaceans could be classified into three groups in spring and four groups in autumn. The differences in crustacean community structure in spring were more significant than in autumn. The results of canonical correspondence analysis (CCA) showed that water depth, bottom temperature, salinity and dissolved oxygen were the main environmental factors affecting species composition and spatial structure of crustaceans in the surveyed area.


Assuntos
Biodiversidade , Braquiúros , Animais , China , Ecossistema , Humanos , Estações do Ano , Temperatura
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