RESUMO
Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
Assuntos
Sistema Livre de Células/metabolismo , Bicamadas Lipídicas/química , Proteínas de Membrana Transportadoras/biossíntese , Proteínas de Plantas/biossíntese , Ácidos Bóricos/metabolismo , Sistema Livre de Células/química , Espectroscopia Dielétrica , Ésteres , Hordeum , Concentração de Íons de Hidrogênio , Immunoblotting , Proteínas de Membrana Transportadoras/química , Fosfatidilcolinas/química , Proteínas de Plantas/química , RNA Mensageiro/genética , RNA de Plantas/genética , Ácido Tióctico/químicaRESUMO
The direct interaction of drugs with the cell membrane is often neglected when drug effects are studied. Systematic investigations are hindered by the complexity of the natural membrane and model membrane systems can offer a useful alternative. Here some examples are reviewed of how model membrane architectures including vesicles, Langmuir monolayers and solid supported membranes can be used to investigate the effects of drug molecules on the membrane structure, and how these interactions can translate into effects on embedded membrane proteins.