RESUMO
The golden Syrian hamster (Mesocricetus auratus) is frequently used as a model to study virulence for several Leptospira species. Onset of an acute lethal infection following inoculation with several pathogenic Leptospira species has been widely adopted for pathogenesis studies. An important exception is the outcome following inoculation of hamsters with live L. borgpetersenii serovar Hardjo, the primary cause of bovine leptospirosis and a cause of human infections. Typically, inoculation of hamsters with L. borgpetersenii serovar Hardjo fails to induce clinical signs of infection. In this study, the authors defined LD(50) and ID(50) for 2 strains of L. borgpetersenii serovar Hardjo: JB197 and 203. Both strains infected hamsters with ID(50) values of approximately 1.5 × 10(2) bacteria yet differed in tissue invasion and interaction with leukocytes, resulting in widely divergent clinical outcomes. Hamsters infected with strain 203 established renal colonization within 4 days postinfection and remained asymptomatic with chronic renal infections similar to cattle infected with serovar Hardjo. In contrast, hamsters infected with strain JB197 developed a rapidly debilitating disease typical of acute leptospirosis common in accidental hosts (eg, humans) with an LD(50) of 3.6 × 10(4) bacteria. Evidence that strain JB197 resides in both extracellular and intracellular environments during hamster infection was obtained. Development of models that result in chronic and acute forms of leptospirosis provides a platform to study L. borgpetersenii pathogenesis and to test vaccines for the prevention of leptospirosis.
Assuntos
Doenças dos Bovinos/microbiologia , Modelos Animais de Doenças , Leptospira/patogenicidade , Leptospirose/patologia , Leucócitos/microbiologia , Mesocricetus , Animais , Bovinos , Doenças dos Bovinos/patologia , Cricetinae , Feminino , Interações Hospedeiro-Patógeno , Humanos , Injeções Intraperitoneais/veterinária , Leptospirose/microbiologia , Dose Letal Mediana , Masculino , Especificidade de Órgãos , VirulênciaRESUMO
Bovine viral diarrhoea viruses (BVDV) are significant pathogens of cattle worldwide. These viruses exist in both non-cytopathic and cytopathic biotypes. Non-cytopathic BVDV can establish persistent lifelong infections in cattle and are a frequent contaminant of biological reagents such as cell cultures and foetal bovine serum. We identified commercially available bovine aortic endothelial cells (BAECs) contaminated with BVDV. In this study, to determine if BVDV alters endothelial gene transcription patterns, serial analysis of gene expression (SAGE) was used to compare gene expression profiles from uninfected and BVDV contaminated BAEC. SAGE is an open ended, quantitative method for characterizing global patterns of transcription. Comparison of expression profiles of BVDV-contaminated and noninfected cells revealed significant increases in the transcription of many genes including P-selectin, tryptophan tRNA synthetase and prostaglandin D2 synthase. These changes were validated by real-time PCR. Additionally, real-time PCR demonstrated that the response to LPS and dsRNA by contaminated cells, as well as cells acutely infected with noncytopathic BVDV, is altered. The altered response may be through the high level of expression of A20 and inhibition of activation of NF-kappaB. BAECs are commonly used as a model to study endothelial cell function in many different systems. As shown here, transcriptional and probable protein changes resulting from BVDV infection significantly alter cellular responses and may have a profound impact on experimental outcome. Transcriptomic analysis provided the initial clues leading to the characterization of this altered function.
Assuntos
Vírus da Diarreia Viral Bovina/fisiologia , Endotélio Vascular/metabolismo , Perfilação da Expressão Gênica , Transcrição Gênica , Animais , Bovinos , Células Cultivadas , Endotélio Vascular/virologia , Reação em Cadeia da PolimeraseRESUMO
The current explosion in new high-throughput technologies arising from microbial and animal genomics studies is enabling the analysis of the genome, transcriptome, and proteome and offers the opportunity to gain a better understanding of the molecular pathways underlying pathogen biology, the host immune system, and host-pathogen interactions. These new tools can be applied to veterinary pathogens to overcome some of the current hurdles in the discovery of highly effective vaccines for farmed livestock and poultry.
Assuntos
Doenças dos Animais/prevenção & controle , Genômica , Proteômica , Vacinas , Animais , Desenho de Fármacos , Análise de Sequência com Séries de Oligonucleotídeos , Vacinas/imunologia , Medicina Veterinária/métodos , Medicina Veterinária/tendênciasRESUMO
Specific mutations in the gene encoding the largest subunit of RNA polymerase II (RpII215) cause a partial transformation of a structure of the third thoracic segment, the capitellum, into the analogous structure of the second thoracic segment, the wing. This mutant phenotype is also caused by genetically reducing the cellular concentration of the transcription factor Ultrabithorax (Ubx). To recover mutations in the 140,000-D second-largest subunit of RNA polymerase II (RpII140) and determine whether any can cause a mutant phenotype similar to Ubx we attempted to identify all recessive-lethal mutable loci in a 340-kilobase deletion including this and other loci. One of the 13 complementation groups in this region encodes RpII140. Three RpII140 alleles cause a transformation of capitellum to wing but unlike RpII215 alleles, only when the concentration of Ubx protein is reduced by mutations in Ubx.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Drosophila , Drosophila/enzimologia , Drosophila/genética , Proteínas de Homeodomínio , RNA Polimerase II/genética , Fatores de Transcrição , Alelos , Animais , Mapeamento Cromossômico , Drosophila/crescimento & desenvolvimento , Genes Letais , Genes Recessivos , Teste de Complementação Genética , Heterozigoto , Mutação , Fenótipo , TemperaturaRESUMO
A lambda gt11 library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (lambda F15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.
Assuntos
Proteínas de Bactérias/biossíntese , Genes Bacterianos , Leptospira/genética , Peptídeo Hidrolases , Sequência de Aminoácidos , Anticorpos Monoclonais , Proteínas de Bactérias/genética , Sequência de Bases , Clonagem Molecular , Primers do DNA , DNA Bacteriano/genética , Escherichia coli/genética , Biblioteca Gênica , Haemophilus influenzae/genética , Leptospira/metabolismo , Dados de Sequência Molecular , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , Homologia de Sequência de AminoácidosRESUMO
The genome structures of several pathogenic spirochetes have recently been determined. The genomes of Borrelia species consist of a linear chromosome of approximately one million base pairs (Mb) and various linear and circular plasmids. Analysis of restriction fragment length polymorphisms and 16S ribosomal RNA sequence data indicate the division of Borrelia burgdorferi into at least three distinct genetic groups. Leptospira interrogans has a circular chromosome 5 Mb in size and a 0.35 Mb extrachromosomal element. Repetitive sequence elements similar to insertion sequences have been identified in the Leptospira interrogans genome. The chromosome of Treponema pallidum subsp. pallidum is circular and has a size of approximately one Mb. Genetic studies conducted to date indicate that B. burgdorferi and L. interrogans have a high degree of genetic diversity, whereas remarkably few genetic differences have been observed among the pathogenic Treponema. Knowledge of the genomic structure of these organisms will serve as a basis for future genetic studies.
Assuntos
Grupo Borrelia Burgdorferi/genética , Leptospira interrogans/genética , Treponema pallidum/genética , Grupo Borrelia Burgdorferi/classificação , DNA Circular/genética , Técnicas In Vitro , Polimorfismo de Fragmento de Restrição , RNA Ribossômico/análise , Mapeamento por RestriçãoRESUMO
A region of the Leptospira interrogans serovar pomona genome encoding DNA replication genes was characterized. This region, designated the ppa-ntrC locus, includes 19 open reading frames and a new insertion sequence, IS1502. Although this locus resembles replication origins from many eubacteria, it lacks several genes common to homologous loci. Some replication-related genes were previously located near rrf, and may have been moved to that location by homologous recombination between short sequence elements common to both loci. Further analysis showed that the ppa-ntrC region has undergone substantial change during spirochete evolution. Transcription analysis using RT-PCR revealed uniquely organized polycistronic mRNAs in the ppa-ntrC locus. The dnaN and recF intergenic region of serovar pomona was different from the homologous sites of 41 L. interrogans serovars by the presence of IS1502. The distribution of IS1502 throughout pathogenic Leptospira species varies. This result suggests that IS1502 may have been recently introduced into Leptospira.
Assuntos
Proteínas de Bactérias/genética , Replicação do DNA , Elementos de DNA Transponíveis , Genes Bacterianos , Leptospira interrogans/genética , Proteínas de Bactérias/metabolismo , Clonagem Molecular , DNA Girase/genética , DNA Bacteriano , Humanos , Leptospira interrogans/metabolismo , Leptospirose/microbiologia , Dados de Sequência Molecular , Fases de Leitura Aberta , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Transcrição GênicaRESUMO
A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and alpha operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-alpha cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution.
Assuntos
Leptospira interrogans/genética , Óperon/genética , Proteínas Ribossômicas/genética , Transcrição Gênica , Proteínas de Bactérias/genética , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Família Multigênica , Análise de Sequência de DNARESUMO
Restriction endonuclease analysis (REA) of genomic DNA can discriminate between many Leptospira interrogans serovars. However, several serovars have similar restriction endonuclease digestion patterns which prohibits accurate identification. This investigation expands previous REA studies of L. interrogans to include serovars in serogroup Tarassovi. Most serovars in this serogroup had characteristic digestion patterns by which they could be identified. However, four of the serovars in this serogroup had similar digestion patterns, thus preventing serovar identification by REA alone. To discriminate between these serovars REA was supplemented with Southern blot analysis. The DNA from each serovar showed similar but unique patterns when hybridized with a probe synthesized from a repetitive sequence element cloned from L. interrogans serovar hardjo type hardjo-bovis. The applicability of this technique to characterize other serogroups was assessed. One hundred sixty six of 190 serovars screened by Southern blot analysis contained sequences which hybridized with the repetitive element probe under conditions of relaxed stringency. These results suggest that Southern blot analysis using this probe will be a valuable supplement for typing L. interrogans.
Assuntos
Sondas de DNA , DNA Bacteriano/análise , Leptospira interrogans/genética , Polimorfismo de Fragmento de Restrição , Animais , Autorradiografia , Southern Blotting , Eletroforese em Gel de Ágar , Hibridização de Ácido Nucleico , Sequências Repetitivas de Ácido Nucleico , Mapeamento por RestriçãoRESUMO
Little is known about the outer membrane structure of Brachyspira hyodysenteriae and Brachyspira pilosicoli or the role of outer membrane proteins (OMPs) in host colonization and the development of disease. The isolation of outer membrane vesicles from B. hyodysenteriae has confirmed that cholesterol is a significant outer membrane constituent and that it may impart unique characteristics to the lipid bilayer structure, including a reduced density. Unique proteins that have been identified in the B. hyodysenteriae outer membrane include the variable surface proteins (Vsp) and lipoproteins such as SmpA and BmpB. While the function of these proteins remains to be determined, there is indirect evidence to suggest that they may be involved in immune evasion. These data may explain the ability of the organism to initiate chronic infection. OMPs may be responsible for the unique attachment of B. pilosicoli to colonic epithelial cells; however, the only B. pilosicoli OMPs that have been identified to date are involved in metabolism. In order to identify further B. pilosicoli OMPs we have isolated membrane vesicle fractions from porcine strain 95-1000 by osmotic lysis and isopycnic centrifugation. The fractions were free of contamination by cytoplasm and flagella and contained outer membrane. Inner membrane contamination was minimal but could not be completely excluded. An abundant 45-kDa, heat-modifiable protein was shown to have significant homology with B. hyodysenteriae Vsp, and monoclonal antibodies were produced that reacted with five B. pilosicoli-specific membrane protein epitopes. The first of these proteins to be characterized is a unique surface-exposed lipoprotein.
Assuntos
Proteínas da Membrana Bacteriana Externa/análise , Brachyspira/química , Lipoproteínas , Lipídeos de Membrana/análise , Infecções por Spirochaetales/veterinária , Animais , Brachyspira/patogenicidade , Brachyspira/ultraestrutura , Brachyspira hyodysenteriae/química , Brachyspira hyodysenteriae/patogenicidade , Membrana Celular/química , Colesterol/análise , Lipopolissacarídeos/análise , Lipídeos de Membrana/classificação , Microscopia Eletrônica , Proteínas de Ligação a RNA/análise , Infecções por Spirochaetales/microbiologiaRESUMO
Nucleic acid hybridization, bacteriologic culture, and a fluorescent antibody test were compared for detection of Leptospira interrogans serovar hardjo type hardjo-bovis in bovine urine. Seventy-five urine samples were collected from pregnant cows challenge exposed with type hardjo-bovis. Twenty samples were collected from steers not exposed to hardjo-bovis. Sediments from each sample were examined, using fluorescent antibodies and a repetitive sequence element nucleic acid probe, to detect the presence of leptospires. Urine samples were processed for bacteriologic culture, using standard techniques. Under laboratory conditions typically used for these techniques, leptospires were detected in 60 of 75 urine samples from challenge exposed cows by nucleic acid hybridization, in 24 samples by fluorescent antibody test, and in 13 samples by bacteriologic culture. Leptospires were not detected in the urine of steers not exposed to hardjo-bovis.
Assuntos
Bacteriúria/veterinária , Doenças dos Bovinos/diagnóstico , Leptospira interrogans/isolamento & purificação , Leptospirose/veterinária , Complicações Infecciosas na Gravidez/veterinária , Animais , Bacteriúria/diagnóstico , Bovinos , Feminino , Imunofluorescência/veterinária , Leptospirose/diagnóstico , Masculino , Hibridização de Ácido Nucleico , Gravidez , Complicações Infecciosas na Gravidez/diagnóstico , Sorotipagem , Vacinação/veterináriaRESUMO
Effectiveness of 2 pentavalent leptospiral vaccines containing Leptospira interrogans serovar hardjo was evaluated for protection of steers from infection with serovar hardjo type hardjo-bovis. The hardjo component of 1 vaccine was prepared from serovar hardjo type hardjoprajitno. The hardjo component of the other vaccine was prepared from serovar hardjo type hardjo-bovis. Two steers were vaccinated once and 4 steers were vaccinated twice with the pentavalent vaccine containing type hardjoprajitno. Four steers were vaccinated once and 4 steers were vaccinated twice with the pentavalent vaccine containing type hardjo-bovis. Four steers were maintained as non-vaccinated controls. Steers given vaccine containing type hardjo-bovis developed higher mean serum microscopic agglutination titers against serovar hardjo than steers given vaccine containing hardjoprajitno. Six months after the first vaccination, all steers were challenge-exposed on 3 occasions by conjunctival instillation of 10(7) serovar hardjo type hardjo-bovis organisms, and on 1 occasion by conjunctival instillation of urine from a steer shedding hardjo-bovis. All control and all vaccinated steers became infected and shed serovar hardjo type hardjo-bovis in the urine. Lesions were detected in kidneys of 3 of 4 nonvaccinated control steers, 5 of 6 steers given hardjoprajitno vaccine, and 6 of 8 steers given hardjo-bovis vaccine. Leptospires were detected in kidneys of 4 of 4 control steers and 13 of 14 vaccinated steers.
Assuntos
Vacinas Bacterianas/imunologia , Doenças dos Bovinos/imunologia , Leptospira interrogans/imunologia , Leptospirose/veterinária , Animais , Formação de Anticorpos , Vacinas Bacterianas/administração & dosagem , Bovinos , Leptospira interrogans/classificação , Leptospirose/imunologia , Masculino , Sorotipagem/veterinária , Especificidade da Espécie , Vacinação/veterináriaRESUMO
Effectiveness of 2 concentrations of a monovalent vaccine containing Leptospira interrogans serovar hardjo type hardjo-bovis was evaluated for protection of heifers from infection with type hardjo-bovis. Nine heifers were given 2 doses of low-dose vaccine (8.32 x 10(8) cells/dose); 9 heifers were given 2 doses of high-dose vaccine (8.32 x 10(9) cells/dose); and 1 steer and 1 heifer were maintained as nonvaccinated controls. Groups of vaccinated cattle were challenge-exposed with serovar hardjo type hardjo-bovis at 7 (n = 6), 11 (n = 6), or 15 (n = 6) weeks after completion of vaccination. All cattle were challenge-exposed by conjunctival instillation of 1 x 10(5) hardjo-bovis cells on 3 consecutive days. Both control and all vaccinated cattle became infected and shed serovar hardjo type hardjo-bovis in their urine. Leptospires were detected in 15 of 16 (94%) urine samples from control cattle and in 124 of 143 (87%) samples from vaccinated cattle. Leptospires were detected in kidneys of 17 of 18 vaccinated cattle and 2 of 2 control cattle and in the uterus or oviducts of 13 of 18 vaccinates and the 1 control heifer.
Assuntos
Vacinas Bacterianas , Doenças dos Bovinos/prevenção & controle , Leptospira interrogans/imunologia , Leptospirose/veterinária , Vacinação/veterinária , Testes de Aglutinação , Animais , Anticorpos Antibacterianos/biossíntese , Bovinos , Tubas Uterinas/microbiologia , Feminino , Imunofluorescência , Técnicas Imunoenzimáticas , Rim/microbiologia , Leptospira interrogans/isolamento & purificação , Leptospirose/prevenção & controle , Masculino , Útero/microbiologiaRESUMO
OBJECTIVE: To compare sensitivity and specificity of various polymerase chain reaction (PCR) assays for detection of Leptospira borgpetersenii serovar hardjo in bovine urine and to compare results of the optimal PCR assay with results of immunofluorescence, nucleic acid hybridization, and bacteriologic culture. ANIMALS: 6 heifers. PROCEDURE: Heifers were exposed to serovar hardjo type hardjo-bovis by conjunctival instillation of 10(6) leptospires on 3 successive days. Urine samples were collected before and after infection. Sensitivity and specificity of 5 PCR assays were compared, to determine the optimal assay for use with bovine urine samples. The optimal PCR assay was then compared with results of bacteriologic culture, nucleic acid hybridization, and immunofluorescence. RESULTS: A PCR assay with the best combination of specificity (100%) and sensitivity (91%) was selected for comparison with the other diagnostic tests. Sensitivity for nucleic acid hybridization was 55%, whereas sensitivity for bacteriologic culture and immunofluorescence was 89 to 93%. CONCLUSIONS AND CLINICAL RELEVANCE: Bacteriologic culture, PCR, and immunofluorescence were sensitive for detection of L borgpetersenii serovar hardjo type hardjo-bovis in urine specimens of cattle, but a single technique was not the most sensitive for each animal tested. Therefore, the use of 2 techniques in combination is warranted for maximal sensitivity for diagnosis.
Assuntos
Doenças dos Bovinos/diagnóstico , Leptospira/isolamento & purificação , Leptospirose/veterinária , Reação em Cadeia da Polimerase/veterinária , Animais , Anticorpos Antibacterianos/química , Técnicas Bacteriológicas , Southern Blotting/veterinária , Bovinos , Doenças dos Bovinos/microbiologia , Primers do DNA/química , DNA Bacteriano/química , DNA Bacteriano/isolamento & purificação , Eletroforese em Gel de Ágar/veterinária , Feminino , Leptospira/genética , Leptospirose/diagnóstico , Microscopia de Fluorescência/veterinária , Hibridização de Ácido Nucleico , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Urina/microbiologiaRESUMO
OBJECTIVE: To evaluate antibiotics for treatment of cattle with leptospirosis caused by Leptospira borgpetersenii serovar hardjo. DESIGN: Randomized controlled trial. ANIMALS: 42 healthy mixed-breed cattle. PROCEDURE: Cattle were inoculated via conjunctival instillation with L. borgpetersenii serovar hardjo. After infection and urinary shedding of L. borgpetersenii were confirmed, cattle were treated with various antibiotics. To determine effectiveness of antibiotic treatment, urinary shedding of L. borgpetersenii was monitored for 4 to 6 weeks after administration of antibiotics, using darkfield microscopic examination, microbial culture, immunofluorescence testing, and a polymerase chain reaction assay. RESULTS: All inoculated cattle developed leptospirosis and shed leptospires in their urine. The following antibiotic treatments resulted in elimination of urinary shedding of leptospires: a single injection of oxytetracycline (20 mg/kg 19 mg/lb] of body weight, IM), tilmicosin (10 mg/kg [4.5 mg/lb], SC), or a combination product that contained dihydrostreptomycin-penicillin G (25 mg/kg [11.4 mg/lb], IM) or multiple injections of ceftiofur sodium (2.2 or 5 mg/kg [1 or 2.3 mg/lb], IM, once daily for 5 days, or 20 mg/kg, IM, once daily for 3 days). CONCLUSIONS AND CLINICAL RELEVANCE: Successful resolution of leptospirosis in cattle by administration of dihydrostreptomycin-penicillin G confirms results obtained by other investigators. Three other antibiotics (oxytetracycline, tilmicosin, and ceftiofur) also were effective for resolving leptospirosis and may be useful substitutes for dihydrostreptomycin, an antibiotic that is no longer available for use in food-producing animals in the United States. Cost, safety, and withdrawal times of these various treatment options need to be considered.
Assuntos
Antibacterianos/uso terapêutico , Bacteriúria/veterinária , Doenças dos Bovinos/tratamento farmacológico , Leptospira/efeitos dos fármacos , Leptospirose/veterinária , Macrolídeos , Tilosina/análogos & derivados , Animais , Antibacterianos/farmacologia , Bacteriúria/tratamento farmacológico , Bacteriúria/microbiologia , Bovinos , Doenças dos Bovinos/microbiologia , Cefalosporinas/farmacologia , Cefalosporinas/uso terapêutico , Sulfato de Di-Hidroestreptomicina/farmacologia , Sulfato de Di-Hidroestreptomicina/uso terapêutico , Quimioterapia Combinada/farmacologia , Quimioterapia Combinada/uso terapêutico , Feminino , Imunofluorescência/veterinária , Leptospira/crescimento & desenvolvimento , Leptospirose/tratamento farmacológico , Leptospirose/microbiologia , Masculino , Oxitetraciclina/farmacologia , Oxitetraciclina/uso terapêutico , Penicilina G/farmacologia , Penicilina G/uso terapêutico , Reação em Cadeia da Polimerase/veterinária , Resultado do Tratamento , Tilosina/farmacologia , Tilosina/uso terapêuticoRESUMO
The size and physical structure of the Leptospira interrogans genome was characterized using contour-clamped homogenous electric field (CHEF) gel electrophoresis. The L. interrogans genome is approximately 4750 kb in size and is composed of two molecular species of DNA: a 4400 kb chromosome; and a 350 kb plasmid, pLIN1. A physical map of the chromosome was constructed with the restriction enzymes NotI and SfiI. A physical map of pLIN1 was constructed with ApaI, NotI, Sse83871, SgrAI, and SmaI. Both the L. interrogans chromosome and pLIN1 are circular.
Assuntos
Mapeamento Cromossômico , DNA Bacteriano/genética , Leptospira interrogans/genética , Plasmídeos , Clonagem Molecular , Enzimas de Restrição do DNA/metabolismo , Genes Bacterianos , Hibridização de Ácido Nucleico , Mapeamento por RestriçãoRESUMO
The nucleotide sequence of IS1533, an insertion sequence-like element cloned from the spirochete Leptospira borgpetersenii, was determined. IS1533 contains imperfect terminal inverted repeats (IVR) of 31 bp flanking a 1402-bp internal sequence. A putative target sequence was identified, and insertion may result in duplication of 2 bp. The internal sequence has a single open reading frame (ORF). IS1533 encodes two proteins (43.5 and 41 kDa) initiating alternatively at either the first or the second AUG codons of the ORF. These proteins are related to a recently recognized family of IS-encoded transposases and bacterial recombinases, all which share a region of homology with the active site of the HIV reverse transcriptase. The IS1533-encoded proteins were expressed in Escherichia coli. Both the 43.5- and 41-kDa proteins bound IS1533 DNA probes in a Southwestern blot assay. These data suggest that one or both proteins function during transposition of IS1533.
Assuntos
Proteínas de Bactérias/genética , Elementos de DNA Transponíveis , Leptospira/genética , Plasmídeos , Sequência de Aminoácidos , Proteínas de Bactérias/biossíntese , Sequência de Bases , Clonagem Molecular , Íntrons , Leptospira/metabolismo , Dados de Sequência Molecular , Mutagênese Insercional , Oligodesoxirribonucleotídeos , Fases de Leitura Aberta , Biossíntese de Proteínas , RNA Mensageiro/biossíntese , RNA Mensageiro/metabolismo , Sequências Repetitivas de Ácido Nucleico , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Regiões Terminadoras Genéticas , Transcrição GênicaRESUMO
Coxiella burnetii was isolated from persistently infected fibroblast host cells by a rapid mechanical lysis technique. Macromolecular synthesis was initiated in these otherwise dormant cells by incubation at pH 4.5. The synthesis of protein proceeded for as long as 24 h. Initiation of protein synthesis in C. burnetii was dependent upon RNA synthesis. Approximately 24 species of polypeptides were synthesized, and some of these appeared to be major synthetic products. Increases in protein biomass of 15 to 30% were calculated to occur during incubation. Inhibition of DNA synthesis affected protein synthesis after 12 h of incubation. The results suggest that although these parasitic bacteria did not grow in the axenic media devised, significant biosynthetic processes occurred.
Assuntos
Proteínas de Bactérias/biossíntese , Coxiella/metabolismo , Aminoácidos/metabolismo , Animais , Proteínas de Bactérias/análise , Linhagem Celular , Coxiella/isolamento & purificação , Cricetinae , DNA Bacteriano/biossíntese , Cinética , Peso Molecular , RNA Bacteriano/biossíntese , Uridina/metabolismoRESUMO
A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restriction enzymes BssHII, EclXI, NotI, SalI, and SmaI. The physical map was used to constructed a linkage map for genes encoding rRNA, flagellum subunit proteins, DNA gyrase, NADH oxidase, and three distinct hemolysins. Several flaB2-related loci, encoding core flagellum subunit proteins, were detected and are dispersed around the chromosome. The rRNA gene organization in S. hyodysenteriae is unusual. S. hyodysenteriae has one gene each for 5S (rrf), 16S (rrs), and 23S (rrl) rRNAs. The rrf and rrl genes are closely linked (within 5 kb), while the rrs gene is about 860 kb from the other two rRNA genes. Using a probe for the S. hyodysenteriae gyrA gene, we identified a possible location for the chromosomal replication origin. The size and genetic organization of the S. hyodysenteriae chromosome are different from those of previously characterized spirochetes.
Assuntos
Brachyspira hyodysenteriae/genética , Mapeamento Cromossômico , Cromossomos Bacterianos , Flagelina , Genes Bacterianos/genética , Proteínas de Bactérias/genética , Sequência de Bases , DNA Topoisomerases Tipo II/genética , DNA Ribossômico/genética , Flagelos , Genoma Bacteriano , Proteínas Hemolisinas/genética , Dados de Sequência Molecular , Complexos Multienzimáticos/genética , NADH NADPH Oxirredutases/genética , Hibridização de Ácido Nucleico , RNA Ribossômico/genética , Mapeamento por RestriçãoRESUMO
Isolates (n = 147) of Leptospira interrogans serovar pomona type kennewicki from cattle, swine, horses, and wildlife were analyzed by DNA restriction endonuclease analysis. Restriction fragment length polymorphisms were identified in DNA digested with HpaII, and the restriction fragment length polymorphisms were correlated with the host animal source of the isolates. These results will be useful in understanding the epidemiology of serovar pomona infections in livestock.