Assuntos
Administração de Caso/organização & administração , Infecções por Coronavirus , Transtornos Mentais/psicologia , Pandemias , Pneumonia Viral , Telemedicina , Betacoronavirus , COVID-19 , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/prevenção & controle , Humanos , Transtornos Mentais/terapia , Saúde Mental , Serviços de Saúde Mental/organização & administração , Pandemias/prevenção & controle , Pneumonia Viral/epidemiologia , Pneumonia Viral/prevenção & controle , SARS-CoV-2Assuntos
Humanos , Pneumonia Viral/prevenção & controle , Pneumonia Viral/epidemiologia , Telemedicina , Infecções por Coronavirus/prevenção & controle , Infecções por Coronavirus/epidemiologia , Administração de Caso/organização & administração , Pandemias/prevenção & controle , Transtornos Mentais/psicologia , Saúde Mental , Betacoronavirus , SARS-CoV-2 , COVID-19 , Transtornos Mentais/terapia , Serviços de Saúde Mental/organização & administraçãoRESUMO
Different species of Leishmania can cause a variety of medically important diseases, whose control and treatment are still health problems. Telomere binding proteins (TBPs) have potential as targets for anti-parasitic chemotherapy because of their importance for genome stability and cell viability. Here, we describe LaTBP1 a protein that has a Myb-like DNA-binding domain, a feature shared by most double-stranded telomeric proteins. Binding assays using full-length and truncated LaTBP1 combined with spectroscopy analysis were used to map the boundaries of the Myb-like domain near to the protein only tryptophan residue. The Myb-like domain of LaTBP1 contains a conserved hydrophobic cavity implicated in DNA-binding activity. A hypothetical model helped to visualize that it shares structural homology with domains of other Myb-containing proteins. Competition assays and chromatin immunoprecipitation confirmed the specificity of LaTBP1 for telomeric and GT-rich DNAs, suggesting that LaTBP1 is a new TBP.
Assuntos
Proteínas de Ligação a DNA/química , DNA/química , Leishmania/metabolismo , Proteínas Oncogênicas v-myb/química , Telômero/química , Sequência de Aminoácidos , Animais , Sítios de Ligação , Dados de Sequência Molecular , Ligação Proteica , Estrutura Terciária de ProteínaRESUMO
The involvement of inducible nitric oxide synthase (iNOS), which plays various roles in the progression of autoimmune diseases, was studied in iNOS knockout (KO) mice and wild-type (WT) controls with respect to experimental autoimmune encephalomyelitis (EAE). The iNOS (KO) mice presented a less severe form of the disease than the WT control mice. Although the levels of TNFalpha decreased in the periphery in both groups, an increase in the number of TNFalpha-positive cells was detected in the central nervous system during the acute phase of EAE in the WT mice, but not in the KO mice. These findings suggest that NO and TNFalpha contribute to the pathogenesis of acute EAE.