Intervention with a combined physical exercise training to reduce oxidative stress of women over 40 years of age.

Exercise training has been shown to be one of the most important lifestyle factor for improving functional performance and health status. Nevertheless, and although some evidence exists about the effects of aerobic training on oxidative stress, there is scarce information concerning the effects of combined exercise training (aerobic and strength training) in oxidative stress. Considering this, the aim of this study was to verify the effects of a combined exercise training in oxidative stress parameters of women over 40 years of age. At baseline, 67 women enrolled in the study and were divided into three groups: younger group (YG, n = 28: 40 to 49 years), middle-aged group (MAG, n = 21: 50 to 59 years) and oldest group (OG, n = 18: above 60 years). These women engaged in a combined exercise training program for 16 weeks, 3 sessions of 60 min per week. At the end of the program, only 31 women (YG: 15; MAG: 8 and OG: 8) were remained in the study and were considered for analysis. Physical assessments (weight, height, body mass index and waist circumference), health and functional parameters (systolic and diastolic blood pressure, fitness tests: supine, latissimus, squat jump, 8 foot up and go test, 30 second chair stand test, and 6 min walk test) and measures of DNA damage (DNA SBs, DNA netFPG), lipid peroxidation (MDA), total antioxidant capacity (TAC) and catalase activity (CAT) were performed before and after the 16-week intervention with combined exercise. The results showed an improvement of overall physical and functional performance as well as a significant decrease in waist perimeter and systolic blood pressure after the exercise program intervention. Regarding the biochemical measures, the exercise training induced a significant decrease in oxidative damage, and a significant increase in the TAC (p < 0.05). The results indicate that combined exercise training induces benefits in functional capacity and reduce damage caused by oxidative stress.

[Leptin: aspects on energetic balance, physical exercise and athletic amenorhea]. / Leptina: aspectos sobre o balanço energético, exercício físico e amenorréia do esforço.

The aim of this manuscript was to review the knowledge about leptin, detailing its relationship with energetic intake and physical activity. Leptin is an adipocyte hormone, recognized mainly for its putative role in control of energy expenditure, food intake, body weight and reproductive function. Leptin has still important peripheral actions, including its role on the ovarian tissue. The intracellular signaling mechanisms are recognized in hypothalamus, but in peripheral tissue are not fully understood. The exercise, when practiced by women, if not appropriately planned according to food intake, can modify the leptin release. When energy imbalances induced by exercise and/or deficient food ingestion occurs, low leptin levels are observed, leading to a reduction in GnRH (gonadotropin-release hormone), in LH (luteinizing hormone) and FSH (follicle-stimulating hormone) in pituitary, and consequently a minor release of ovarian estrogens. This process is named hypothalamic amenorrhea, and has repercussions in the woman's health. In this perspective, it is important to emphasize the need to evaluate the energy expenditure from exercise and to formulate adequate alimentary plans to these individuals.

Effect of food restriction and intense physical training on estrous cyclicity and plasma leptin concentrations in rats.

Intense physical training and dietary energy restriction have been associated with consequences such as nutritional amenorrhea. We investigated the effects of intense physical training, food restriction or the combination of both strategies on estrous cyclicity in female rats, and the relationship between leptin ad these effects. Twenty-seven female Wistar rats were distributed into four groups: SF: sedentary, fed ad libitum; SR: sedentary subjected to 50% food restriction (based on the food intake of their fed counterparts); TF: trained (physical training on a motor treadmill with a gradual increase in speed and time), fed ad libitum; TR: trained with 50% food restriction. We analysed estrous cyclicity, plasma leptin and estradiol as well as chemical composition of the carcass, body weight variation, and weight of ovaries and perirenal adipose tissue. Data demonstrate that physical training alone was not responsible for significant modifications in either carcass chemical composition or reproductive function. Food restriction reduced leptin levels in all animals and interrupted the estrous cyclicity in some animals, but only the combination of food restriction and physical training was capable of interrupting the estrous cyclicity in all animals. Leptin was not directly related to estrous cyclicity. From our findings, it may be concluded that there is an additive or synergistic effect of energy intake restriction and energy expenditure by intense physical training on estrous cyclicity. Leptin appears to be one among others factors related to estrous cycle, but it probably acts indirectly.