[Cytogenetic studies of chorionic villi tissue of abortion samples]. / Zytogenetische Untersuchungen von Chorionzottengewebe aus Abortmaterial.

PIP: The technique of overnight incubation of chorionic villi tissue from abortion samples makes it possible to prepare a fetal karyogram within a day, in contrast to the 4-6 weeks required for conventional tissue culture techniques. Previously cytogenic analyses could be carried out only in exceptional cases because of the resources required for conventional tissue culture. In 211 cases of spontaneous abortion studied by the overnight culture technique, 65% showed pathological karyotypes. A detailed action plan for obtaining chorionic villi tissue by the gynecologist minimizes the chances that the tissue culture will be contaminated by maternal tissue. The various types of chromosome anomalies which appeared in the short incubation karyograms corresponds roughly with the distribution pattern obtained in other investigations of aborted material. Trisomy karyotypes dominated (57%) in which chromosomes 16 and 22 were most frequently involved. Mosaic and triploid anomalies were the 2nd and 3d most common.^ieng

[Structural changes in the chorionic terminal villi and endometrial decidual tissue in spontaneous abortions]. / Strukturnye izmeneniia terminal'nykh vorsin khoriona i detsidual''noi tkani éndometriia pri samoproizvol'nykh vykidyshakh.

PIP: In order to determine structural changes of the chorionic villi and the deciduous tissue during spontaneous abortions, a morphological study of the mucus of the corpus uteri of 80 women, aged 19-37, was performed. They were divided into 3 groups: 1) induced abortion within 5-12 weeks, 2) spontaneous abortion between 5-8 weeks, 3) abortion occurring despite therapy. The women had begun taking hormonal preparations and vitamins in order to continue pregnancy. A detailed analysis of the structure of the endometrial deciduous tissue revealed 4 types of cells. 1 type was characterized by a stretched form with homogeneous basophilous rounded cytoplasm and a large hyperchromatic center. Another type had a rounded form and an increased center. A 3rd type displayed a polygonal outline and increased cytoplasm. A 4th type consisted of vacuolated cytoplasm and a pyknotic center. These types were assigned respectively to immature, maturing, mature, and dystrophic types. An evaluation of the functional conditions of the chorionic villi and the level of structural maturity of the cells of the deciduous endometrial tissue was performed. During the 1st 5-6 weeks of pregnancy these were either unmatured and or in the process of maturing. After 7-8 weeks the number of mature villi greatly increased and after 9-10 weeks they become very predominant, while the terminal chorionic villi changed hypoplastically and dystrophically. In order to establish the reason for the pathological change of the terminal chorionic villi, a comparison was made of the levels of pronounced hypoplastic, dystrophic and necrotic change of the villi with the level of structural differentiation of the cells of the endometrial deciduous tissue in correlation with the development and ceasing of pregnancy. The hypoplasia of the terminal chorionic villi increased during the course of pregnancy. It was found that hypoplasia of terminal chorionic villi is a characteristic morphological indication of spontaneous abortion.^ieng